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Title: Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Background: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. Results: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. Conclusion: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.
Authors:
 [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [2] ;  [4] ;  [1] ;  [1] ;  [2] ;  [5] ;  [2] ;  [5] ;  [6] ;  [3] ;  [1] ;  [7] ;  [2] ;  [2] ;  [4] more »;  [7] ;  [1] « less
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Davis, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  4. Univ. of Oklahoma, Norman, OK (United States)
  5. Univ. of California, Davis, CA (United States)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Davis, CA (United States); Jawaharlal Nehru Univ., New Delhi (India)
  7. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
BMC Biotechnology (Online)
Additional Journal Information:
Journal Name: BMC Biotechnology (Online); Journal Volume: 18; Journal Issue: 1; Journal ID: ISSN 1472-6750
Publisher:
BioMed Central
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1477379

Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., and Ronald, Pamela C.. Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification. United States: N. p., Web. doi:10.1186/s12896-018-0464-8.
Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., & Ronald, Pamela C.. Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification. United States. doi:10.1186/s12896-018-0464-8.
Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., and Ronald, Pamela C.. 2018. "Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification". United States. doi:10.1186/s12896-018-0464-8. https://www.osti.gov/servlets/purl/1477379.
@article{osti_1477379,
title = {Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification},
author = {Li, Guotian and Jones, Kyle C. and Eudes, Aymerick and Pidatala, Venkataramana R. and Sun, Jian and Xu, Feng and Zhang, Chengcheng and Wei, Tong and Jain, Rashmi and Birdseye, Devon and Canlas, Patrick E. and Baidoo, Edward E. K. and Duong, Phat Q. and Sharma, Manoj K. and Singh, Seema and Ruan, Deling and Keasling, Jay D. and Mortimer, Jenny C. and Loqué, Dominique and Bartley, Laura E. and Scheller, Henrik V. and Ronald, Pamela C.},
abstractNote = {Background: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. Results: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. Conclusion: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.},
doi = {10.1186/s12896-018-0464-8},
journal = {BMC Biotechnology (Online)},
number = 1,
volume = 18,
place = {United States},
year = {2018},
month = {9}
}

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