Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes
Abstract
In this paper, information about the distributions of cholesterol and sphingolipids within the plasma membranes of mammalian cells provides insight into the roles of these molecules in membrane function. In this report, high-resolution secondary ion mass spectrometry was used to image the distributions of metabolically incorporated rare isotope-labeled sphingolipids and cholesterol on the surfaces of nonpolarized epithelial cells. Sphingolipid domains that were not enriched with cholesterol were detected in the plasma membranes of subconfluent Madin-Darby canine kidney cells. Surprisingly, cholesterol-enriched sphingolipid patches were observed on the substrate adjacent to these cells. Based on the shapes of these cholesterol-enriched sphingolipid patches on the substrate and their proximity to cellular projections, we hypothesize that they are deposits of membranous particles released by the cell.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1567872
- Alternate Identifier(s):
- OSTI ID: 1476856
- Report Number(s):
- LLNL-JRNL-745101
Journal ID: ISSN 0005-2736; S0005273618301263; PII: S0005273618301263
- Grant/Contract Number:
- 08-LW-015; AC52-07NA27344
- Resource Type:
- Published Article
- Journal Name:
- Biochimica et Biophysica Acta. Biomembranes
- Additional Journal Information:
- Journal Name: Biochimica et Biophysica Acta. Biomembranes Journal Volume: 1860 Journal Issue: 10; Journal ID: ISSN 0005-2736
- Publisher:
- Elsevier
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Sphingolipids; Cholesterol; Madin-Darby canine kidney cells; Lipid domains; Secondary ion mass spectrometry imaging; Stable isotope labeling
Citation Formats
Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L. Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes. Netherlands: N. p., 2018.
Web. doi:10.1016/j.bbamem.2018.04.008.
Yeager, Ashley N., Weber, Peter K., & Kraft, Mary L. Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes. Netherlands. https://doi.org/10.1016/j.bbamem.2018.04.008
Yeager, Ashley N., Weber, Peter K., and Kraft, Mary L. Mon .
"Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes". Netherlands. https://doi.org/10.1016/j.bbamem.2018.04.008.
@article{osti_1567872,
title = {Cholesterol is enriched in the sphingolipid patches on the substrate near nonpolarized MDCK cells, but not in the sphingolipid domains in their plasma membranes},
author = {Yeager, Ashley N. and Weber, Peter K. and Kraft, Mary L.},
abstractNote = {In this paper, information about the distributions of cholesterol and sphingolipids within the plasma membranes of mammalian cells provides insight into the roles of these molecules in membrane function. In this report, high-resolution secondary ion mass spectrometry was used to image the distributions of metabolically incorporated rare isotope-labeled sphingolipids and cholesterol on the surfaces of nonpolarized epithelial cells. Sphingolipid domains that were not enriched with cholesterol were detected in the plasma membranes of subconfluent Madin-Darby canine kidney cells. Surprisingly, cholesterol-enriched sphingolipid patches were observed on the substrate adjacent to these cells. Based on the shapes of these cholesterol-enriched sphingolipid patches on the substrate and their proximity to cellular projections, we hypothesize that they are deposits of membranous particles released by the cell.},
doi = {10.1016/j.bbamem.2018.04.008},
journal = {Biochimica et Biophysica Acta. Biomembranes},
number = 10,
volume = 1860,
place = {Netherlands},
year = {Mon Oct 01 00:00:00 EDT 2018},
month = {Mon Oct 01 00:00:00 EDT 2018}
}
https://doi.org/10.1016/j.bbamem.2018.04.008
Web of Science
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