Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A

He, Qiaoning; Yang, Yongfu; Yang, Shihui; Donohoe, Bryon S.; Van Wychen, Stefanie; Zhang, Min; Himmel, Michael E.; Knoshaug, Eric P.

doi:10.1186/s13068-018-1256-z

Title: Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A

Abstract

Here, the model yeast, Saccharomyces cerevisiae, is not known to be oleaginous. However, an industrial wild-type strain, D5A, was shown to accumulate over 20% storage lipids from glucose when growth is nitrogen-limited compared to no more than 7% lipid accumulation without nitrogen stress. To elucidate the mechanisms of S. cerevisiae D5A oleaginicity, we compared physiological and metabolic changes; as well as the transcriptional profiles of the oleaginous industrial strain, D5A, and a non-oleaginous laboratory strain, BY4741, under normal and nitrogen-limited conditions using analytic techniques and next-generation sequencing-based RNA-Seq transcriptomics. Transcriptional levels for genes associated with fatty acid biosynthesis, nitrogen metabolism, amino acid catabolism, as well as the pentose phosphate pathway and ethanol oxidation in central carbon (C) metabolism, were up-regulated in D5A during nitrogen deprivation. Despite increased carbon flux to lipids, most gene-encoding enzymes involved in triacylglycerol (TAG) assembly were expressed at similar levels regardless of the varying nitrogen concentrations in the growth media and strain backgrounds. Phospholipid turnover also contributed to TAG accumulation through increased precursor production with the down-regulation of subsequent phospholipid synthesis steps. Our results also demonstrated that nitrogen assimilation via the glutamate-glutamine pathway and amino acid metabolism, as well as the fluxes of carbon and reductantsmore »« less

Authors:: He, Qiaoning; Yang, Yongfu; Yang, Shihui; Donohoe, Bryon S.; Van Wychen, Stefanie; Zhang, Min; Himmel, Michael E.; Knoshaug, Eric P.

Publication Date:: Mon Sep 24 00:00:00 EDT 2018

Research Org.:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

OSTI Identifier:: 1618737

Alternate Identifier(s):: OSTI ID: 1476705

Report Number(s):: NREL/JA-5100-71803
Journal ID: ISSN 1754-6834; 258; PII: 1256

Grant/Contract Number:: AC36-08GO28308; 2018ACA149

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 09 BIOMASS FUELS; oleaginous yeast; Saccharomyces cerevisiae; transcriptomics; RNA-Seq; lipid accumulation; triacylglycerol (TAG); nitrogen assimilation

Citation Formats


                    He, Qiaoning, Yang, Yongfu, Yang, Shihui, Donohoe, Bryon S., Van Wychen, Stefanie, Zhang, Min, Himmel, Michael E., and Knoshaug, Eric P. Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A.  Netherlands: N. p., 2018. 
Web.  doi:10.1186/s13068-018-1256-z.

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                    He, Qiaoning, Yang, Yongfu, Yang, Shihui, Donohoe, Bryon S., Van Wychen, Stefanie, Zhang, Min, Himmel, Michael E., & Knoshaug, Eric P. Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A.  Netherlands.  https://doi.org/10.1186/s13068-018-1256-z

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                    He, Qiaoning, Yang, Yongfu, Yang, Shihui, Donohoe, Bryon S., Van Wychen, Stefanie, Zhang, Min, Himmel, Michael E., and Knoshaug, Eric P. Mon .  
"Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A".  Netherlands.  https://doi.org/10.1186/s13068-018-1256-z.

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@article{osti_1618737,

  title        = {Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A},

  author       = {He, Qiaoning and Yang, Yongfu and Yang, Shihui and Donohoe, Bryon S. and Van Wychen, Stefanie and Zhang, Min and Himmel, Michael E. and Knoshaug, Eric P.},

  abstractNote = {Here, the model yeast, Saccharomyces cerevisiae, is not known to be oleaginous. However, an industrial wild-type strain, D5A, was shown to accumulate over 20% storage lipids from glucose when growth is nitrogen-limited compared to no more than 7% lipid accumulation without nitrogen stress. To elucidate the mechanisms of S. cerevisiae D5A oleaginicity, we compared physiological and metabolic changes; as well as the transcriptional profiles of the oleaginous industrial strain, D5A, and a non-oleaginous laboratory strain, BY4741, under normal and nitrogen-limited conditions using analytic techniques and next-generation sequencing-based RNA-Seq transcriptomics. Transcriptional levels for genes associated with fatty acid biosynthesis, nitrogen metabolism, amino acid catabolism, as well as the pentose phosphate pathway and ethanol oxidation in central carbon (C) metabolism, were up-regulated in D5A during nitrogen deprivation. Despite increased carbon flux to lipids, most gene-encoding enzymes involved in triacylglycerol (TAG) assembly were expressed at similar levels regardless of the varying nitrogen concentrations in the growth media and strain backgrounds. Phospholipid turnover also contributed to TAG accumulation through increased precursor production with the down-regulation of subsequent phospholipid synthesis steps. Our results also demonstrated that nitrogen assimilation via the glutamate-glutamine pathway and amino acid metabolism, as well as the fluxes of carbon and reductants from central C metabolism, are integral to the general oleaginicity of D5A, which resulted in the enhanced lipid storage during nitrogen deprivation. This work demonstrated the disequilibrium and rebalance of carbon and nitrogen contribution to the accumulation of lipids in the oleaginous yeast S. cerevisiae D5A. Rather than TAG assembly from acyl groups, the major switches for the enhanced lipid accumulation of D5A (i.e., fatty acid biosynthesis) are the increases of cytosolic pools of acetyl-CoA and NADPH, as well as alternative nitrogen assimilation.},

  doi          = {10.1186/s13068-018-1256-z},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 11,

  place        = {Netherlands},

  year         = {Mon Sep 24 00:00:00 EDT 2018},

  month        = {Mon Sep 24 00:00:00 EDT 2018}

}

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https://doi.org/10.18331/BRJ2018.5.2.3
Comparison of nitrogen depletion and repletion on lipid production in yeast and fungal species

Journal Article Yang, Shihui ; Wang, Wei ; Wei, Hui ; ... - Energies

Although it is well known that low nitrogen stimulates lipid accumulation, especially for algae and some oleaginous yeast, few studies have been conducted in fungal species, especially on the impact of different nitrogen deficiency strategies. In this study, we use two promising consolidated bioprocessing (CBP) candidates to examine the impact of two nitrogen deficiency strategies on lipid production, which are the extensively investigated oleaginous yeast Yarrowia lipolytica, and the commercial cellulase producer Trichoderma reesei. We first utilized bioinformatics approaches to reconstruct the fatty acid metabolic pathway and demonstrated the presence of a triacylglycerol (TAG) biosynthesis pathway in Trichoderma reesei. Wemore »« less
Cited by 9
https://doi.org/10.3390/en9090685

Full Text Available
Characterization of key triacylglycerol biosynthesis processes in rhodococci

Journal Article Amara, Sawsan ; Seghezzi, Nicolas ; Otani, Hiroshi ; ... - Scientific Reports

In this study, oleaginous microorganisms have considerable potential for biofuel and commodity chemical production. Under nitrogen-limitation, Rhodococcus jostii RHA1 grown on benzoate, an analog of lignin depolymerization products, accumulated triacylglycerols (TAGs) to 55% of its dry weight during transition to stationary phase, with the predominant fatty acids being C16:0 and C17:0. Transcriptomic analyses of RHA1 grown under conditions of N-limitation and N-excess revealed 1,826 dysregulated genes. Genes whose transcripts were more abundant under N-limitation included those involved in ammonium assimilation, benzoate catabolism, fatty acid biosynthesis and the methylmalonyl-CoA pathway. Of the 16 atf genes potentially encoding diacylglycerol O-acyltransferases, atf8 transcriptsmore »« less
Cited by 46
https://doi.org/10.1038/srep24985

Full Text Available

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MOESM1 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
figure, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 PPTX file (755.4 kB)
DOI: 10.6084/m9.figshare.7126256.v1
This figure is a supplement to the current record

MOESM2 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (10.91 kB)
DOI: 10.6084/m9.figshare.7126262
This dataset is a supplement to the current record

MOESM2 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (10.91 kB)
DOI: 10.6084/m9.figshare.7126262.v1
This dataset is a supplement to the current record

MOESM3 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (1.2 MB)
DOI: 10.6084/m9.figshare.7126268
This dataset is a supplement to the current record

MOESM3 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (1.2 MB)
DOI: 10.6084/m9.figshare.7126268.v1
This dataset is a supplement to the current record

MOESM4 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (1.44 MB)
DOI: 10.6084/m9.figshare.7126274
This dataset is a supplement to the current record

MOESM4 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (1.44 MB)
DOI: 10.6084/m9.figshare.7126274.v1
This dataset is a supplement to the current record

MOESM5 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (1.02 MB)
DOI: 10.6084/m9.figshare.7126277
This dataset is a supplement to the current record

MOESM5 of Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A
dataset, September 2018

Donohoe, Bryon; Wychen, Stefanie; Zhang, Min
figshare-Supplementary information for journal article at DOI: 10.1186/s13068-018-1256-z, 1 XLSX file (1.02 MB)
DOI: 10.6084/m9.figshare.7126277.v1
This dataset is a supplement to the current record