Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater
Abstract
Oil biodegradation as a weathering process has been extensively investigated over the years, especially after the Deepwater Horizon blowout. In this study, we performed microcosm experiments at 5 °C with chemically dispersed oil in non-amended seawater. We link biodegradation processes with microbial community and metagenome dynamics and explain the succession based on substrate specialization. Reconstructed genomes and 16S rRNA gene analysis revealed that Bermanella and Zhongshania were the main contributors to initial n-alkane breakdown, while subsequent abundances of Colwellia and microorganisms closely related to Porticoccaceae were involved in secondary n-alkane breakdown and beta-oxidation. Cycloclasticus, Porticoccaceae and Spongiiabcteraceae were associated with degradation of mono- and poly-cyclic aromatics. Successional pattern of genes coding for hydrocarbon degrading enzymes at metagenome level, and reconstructed genomic content, revealed a high differentiation of bacteria involved in hydrocarbon biodegradation. A cooperation among oil degrading microorganisms is thus needed for the complete substrate transformation.
- Authors:
-
[1];
- Norwegian Univ. of Science and Technology, Trondheim (Norway)
- SINTEF OCEAN, Trondheim (Norway)
- Univ. of Tennessee, Knoxville, TN (United States)
- Michigan Technological Univ., Houghton, MI (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1476403
- Alternate Identifier(s):
- OSTI ID: 1479397
- Grant/Contract Number:
- AC05-00OR22725; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Marine Pollution Bulletin
- Additional Journal Information:
- Journal Volume: 129; Journal Issue: 1; Journal ID: ISSN 0025-326X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Ribicic, Deni, Netzer, Roman, Hazen, Terry C., Techtmann, Stephen M., Drabløs, Finn, and Brakstad, Odd Gunnar. Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater. United States: N. p., 2018.
Web. doi:10.1016/j.marpolbul.2018.02.034.
Ribicic, Deni, Netzer, Roman, Hazen, Terry C., Techtmann, Stephen M., Drabløs, Finn, & Brakstad, Odd Gunnar. Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater. United States. https://doi.org/10.1016/j.marpolbul.2018.02.034
Ribicic, Deni, Netzer, Roman, Hazen, Terry C., Techtmann, Stephen M., Drabløs, Finn, and Brakstad, Odd Gunnar. Fri .
"Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater". United States. https://doi.org/10.1016/j.marpolbul.2018.02.034. https://www.osti.gov/servlets/purl/1476403.
@article{osti_1476403,
title = {Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater},
author = {Ribicic, Deni and Netzer, Roman and Hazen, Terry C. and Techtmann, Stephen M. and Drabløs, Finn and Brakstad, Odd Gunnar},
abstractNote = {Oil biodegradation as a weathering process has been extensively investigated over the years, especially after the Deepwater Horizon blowout. In this study, we performed microcosm experiments at 5 °C with chemically dispersed oil in non-amended seawater. We link biodegradation processes with microbial community and metagenome dynamics and explain the succession based on substrate specialization. Reconstructed genomes and 16S rRNA gene analysis revealed that Bermanella and Zhongshania were the main contributors to initial n-alkane breakdown, while subsequent abundances of Colwellia and microorganisms closely related to Porticoccaceae were involved in secondary n-alkane breakdown and beta-oxidation. Cycloclasticus, Porticoccaceae and Spongiiabcteraceae were associated with degradation of mono- and poly-cyclic aromatics. Successional pattern of genes coding for hydrocarbon degrading enzymes at metagenome level, and reconstructed genomic content, revealed a high differentiation of bacteria involved in hydrocarbon biodegradation. A cooperation among oil degrading microorganisms is thus needed for the complete substrate transformation.},
doi = {10.1016/j.marpolbul.2018.02.034},
journal = {Marine Pollution Bulletin},
number = 1,
volume = 129,
place = {United States},
year = {Fri Mar 23 00:00:00 EDT 2018},
month = {Fri Mar 23 00:00:00 EDT 2018}
}
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