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Title: New Structural Biology Facilities at NSLS-II

Abstract

The National Synchrotron Light Source II (NSLS-II), a Department of Energy (DOE) Office of Science User Facility located at Brookhaven National Laboratory offers an advanced suite of beamlines dedicated to structural biology research for investigating problems ranging from the atomic to the cellular scale. The beamlines are now available for general users, while adjacent wet and dry laboratories will become available soon. These beamlines offer X-ray diffraction, scattering, footprinting, spectroscopy, and imaging techniques that capitalize on NSLS-II's brightness.

Authors:
 [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOD; National Institutes of Health (NIH); National Science Foundation (NSF); New York Structural Biology Center (NYSBC), New York City, NY (United States); Stony Brook Univ., NY (United States); Case Western Reserve Univ., Cleveland, OH (United States); Arizona State Univ., Mesa, AZ (United States)
OSTI Identifier:
1476289
Report Number(s):
BNL-209123-2018-JAAM
Journal ID: ISSN 0894-0886
Grant/Contract Number:  
SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
Synchrotron Radiation News
Additional Journal Information:
Journal Volume: 31; Journal Issue: 5; Journal ID: ISSN 0894-0886
Publisher:
Taylor & Francis
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 43 PARTICLE ACCELERATORS

Citation Formats

Berman, Lonny. New Structural Biology Facilities at NSLS-II. United States: N. p., 2018. Web. doi:10.1080/08940886.2018.1506245.
Berman, Lonny. New Structural Biology Facilities at NSLS-II. United States. doi:10.1080/08940886.2018.1506245.
Berman, Lonny. Tue . "New Structural Biology Facilities at NSLS-II". United States. doi:10.1080/08940886.2018.1506245. https://www.osti.gov/servlets/purl/1476289.
@article{osti_1476289,
title = {New Structural Biology Facilities at NSLS-II},
author = {Berman, Lonny},
abstractNote = {The National Synchrotron Light Source II (NSLS-II), a Department of Energy (DOE) Office of Science User Facility located at Brookhaven National Laboratory offers an advanced suite of beamlines dedicated to structural biology research for investigating problems ranging from the atomic to the cellular scale. The beamlines are now available for general users, while adjacent wet and dry laboratories will become available soon. These beamlines offer X-ray diffraction, scattering, footprinting, spectroscopy, and imaging techniques that capitalize on NSLS-II's brightness.},
doi = {10.1080/08940886.2018.1506245},
journal = {Synchrotron Radiation News},
number = 5,
volume = 31,
place = {United States},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Figures / Tables:

Figure 1 Figure 1: Manipulation of microcrystals for micro-crystallography. (a) Patterned well mount. Inset: A high-resolution image of microwells to show their shape and dimensions. For better visualization, the 2 μm holes are highlighted by white circles. (b) A generic procedure for harvesting and cooling microcrystals. A micropipette is used to aspiratemore » microcrystals and the microcrystal droplet is deposited onto the top side of the well mount; solvent is then removed from the bottom side by using a fiber paper and the well mount is plunged into liquid nitrogen for cooling. (c) The 1.0 x 1.5 μm FMX beam profile measured at 12.6 kev. (d) A light microscope view of a well mount loaded with microcrystals in an orientation ready for raster scanning. (e) A raster-scan heat map of diffraction intensity from microcrystals on a well mount. Reproduced with permission courtesy of G. Guo et al., IUCrJ .1, 238-246 (2018).« less

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