3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells
Abstract
The insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway of an inducible plant secretory system. The leaf glands were induced with bovine serum albumin to secrete proteases that were characterized via zymogram activity gels over a 6-day period. The accompanying morphological changes of the endoplasmic reticulum (ER) and Golgi were analyzed using 3D electron tomography of glands preserved by high-pressure freezing/freeze substitution methods. Secretion of multiple cysteine and aspartic proteases occurred biphasically. The majority of the Golgi was organized in clusters consisting of 3–6 stacks surrounded by a cage-like system of ER cisternae. In these clusters, all Golgi stacks were oriented with their cis-most C1 cisterna facing an ER export site. The C1 Golgi cisternae varied in size and shape consistent with the hypothesis that they form de novo. Following induction, the number of ER-bound polysomes doubled, but no increase in COPII vesicles was observed. Golgi changes included a reduction in the number of cisternae per stack and a doubling of cisternal volume without increased surface area. Polysaccharide molecules that form the sticky slime cause swelling of the trans and trans Golgi network (TGN) cisternae. Peeling of the trans-most cisternae gives rise to free TGNmore »
- Authors:
-
[1];
- Univ. of Colorado, Boulder, CO (United States). Molecular, Cellular & Developmental Biology
- National Univ. of La Plata (Argentina). Inst. of Plant Physiology (INFIVE)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Exxel Pharma, Inc., Aurora, CO (United States)
- Wilson Sonsini Goodrich & Rosati, San Francisco, CA (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
- Sponsoring Org.:
- USDOE; National Inst. of Health (NIH) (United States)
- OSTI Identifier:
- 1475519
- Report Number(s):
- NREL/JA-2700-72519
Journal ID: ISSN 2241-5793
- Grant/Contract Number:
- AC36-08GO28308; GM-61306
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Biological Research-Thessaloniki (Online)
- Additional Journal Information:
- Journal Name: Journal of Biological Research-Thessaloniki (Online); Journal Volume: 25; Journal ID: ISSN 2241-5793
- Publisher:
- BioMed Central Ltd (BMC)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Venus flytrap; Golgi; trans Golgi network; endoplasmic reticulum; transmission electron microscopy; electron tomography
Citation Formats
Gergely, Zachary R., Martinez, Dana E., Donohoe, Bryon S., Mogelsvang, Soren, Herder, Rachel, and Staehelin, L. Andrew. 3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells. United States: N. p., 2018.
Web. doi:10.1186/s40709-018-0086-2.
Gergely, Zachary R., Martinez, Dana E., Donohoe, Bryon S., Mogelsvang, Soren, Herder, Rachel, & Staehelin, L. Andrew. 3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells. United States. https://doi.org/10.1186/s40709-018-0086-2
Gergely, Zachary R., Martinez, Dana E., Donohoe, Bryon S., Mogelsvang, Soren, Herder, Rachel, and Staehelin, L. Andrew. Wed .
"3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells". United States. https://doi.org/10.1186/s40709-018-0086-2. https://www.osti.gov/servlets/purl/1475519.
@article{osti_1475519,
title = {3D electron tomographic and biochemical analysis of ER, Golgi and trans Golgi network membrane systems in stimulated Venus flytrap (Dionaea muscipula) glandular cells},
author = {Gergely, Zachary R. and Martinez, Dana E. and Donohoe, Bryon S. and Mogelsvang, Soren and Herder, Rachel and Staehelin, L. Andrew},
abstractNote = {The insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway of an inducible plant secretory system. The leaf glands were induced with bovine serum albumin to secrete proteases that were characterized via zymogram activity gels over a 6-day period. The accompanying morphological changes of the endoplasmic reticulum (ER) and Golgi were analyzed using 3D electron tomography of glands preserved by high-pressure freezing/freeze substitution methods. Secretion of multiple cysteine and aspartic proteases occurred biphasically. The majority of the Golgi was organized in clusters consisting of 3–6 stacks surrounded by a cage-like system of ER cisternae. In these clusters, all Golgi stacks were oriented with their cis-most C1 cisterna facing an ER export site. The C1 Golgi cisternae varied in size and shape consistent with the hypothesis that they form de novo. Following induction, the number of ER-bound polysomes doubled, but no increase in COPII vesicles was observed. Golgi changes included a reduction in the number of cisternae per stack and a doubling of cisternal volume without increased surface area. Polysaccharide molecules that form the sticky slime cause swelling of the trans and trans Golgi network (TGN) cisternae. Peeling of the trans-most cisternae gives rise to free TGN cisternae. One day after gland stimulation, the free TGNs were frequently associated with loose groups of oriented actin-like filaments which were not seen in any other samples. These findings suggest that the secretory apparatus of resting gland cells is “overbuilt” to enable the cells to rapidly up-regulate lytic enzyme production and secretion in response to prey trapping.},
doi = {10.1186/s40709-018-0086-2},
journal = {Journal of Biological Research-Thessaloniki (Online)},
number = ,
volume = 25,
place = {United States},
year = {2018},
month = {8}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 8 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Membrane Traffic Within the Golgi Apparatus
journal, November 2009
- Glick, Benjamin S.; Nakano, Akihiko
- Annual Review of Cell and Developmental Biology, Vol. 25, Issue 1
Mechano-Stimulation Triggers Turgor Changes Associated with Trap Closure in the Darwin Plant Dionaea muscipula
journal, April 2014
- Escalante-Pérez, María; Scherzer, Sönke; Al-Rasheid, Khaled A. S.
- Molecular Plant, Vol. 7, Issue 4
The Venus Flytrap Dionaea muscipula Counts Prey-Induced Action Potentials to Induce Sodium Uptake
journal, February 2016
- Böhm, Jennifer; Scherzer, Sönke; Krol, Elzbieta
- Current Biology, Vol. 26, Issue 3
Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage
journal, June 2008
- Young, Robin E.; McFarlane, Heather E.; Hahn, Michael G.
- The Plant Cell, Vol. 20, Issue 6
The Secretory Cycle of Dionaea Muscipula Ellis iv. the Enzymology of the Secretion
journal, December 1980
- Robins, R. J.; Juniper, B. E.
- New Phytologist, Vol. 86, Issue 4
INF2-Mediated Severing through Actin Filament Encirclement and Disruption
journal, January 2014
- Gurel, Pinar S.; Ge, Peng; Grintsevich, Elena E.
- Current Biology, Vol. 24, Issue 2
Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat
journal, December 2004
- Contreras, Inmaculada; Yang, Yaodong; Robinson, David G.
- Plant and Cell Physiology, Vol. 45, Issue 12
Endocytic and Secretory Traffic in Arabidopsis Merge in the Trans-Golgi Network/Early Endosome, an Independent and Highly Dynamic Organelle
journal, April 2010
- Viotti, Corrado; Bubeck, Julia; Stierhof, York-Dieter
- The Plant Cell, Vol. 22, Issue 4
Acid phosphatase localization in the digestive glands of Dionaea muscipula Ellis flytraps.
journal, April 1985
- Henry, Y.; Steer, M. W.
- Journal of Histochemistry & Cytochemistry, Vol. 33, Issue 4
A distinct class of vesicles derived from the trans -Golgi mediates secretion of xylogalacturonan in the root border cell
journal, October 2017
- Wang, Pengfei; Chen, Xinshi; Goldbeck, Cameron
- The Plant Journal, Vol. 92, Issue 4
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography
journal, August 2008
- Staehelin, L. Andrew; Kang, Byung-Ho
- Plant Physiology, Vol. 147, Issue 4
Isolation of Intact Protein Storage Vacuoles from Barley Aleurone (Identification of Aspartic and Cysteine Proteases)
journal, February 1996
- Bethke, P. C.; Hillmer, S.; Jones, R. L.
- Plant Physiology, Vol. 110, Issue 2
Dynamics of COPII Vesicles and the Golgi Apparatus in Cultured Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with Endoplasmic Reticulum Exit Sites
journal, April 2005
- Yang, Yao-dong; Elamawi, Rabab; Bubeck, Julia
- The Plant Cell, Vol. 17, Issue 5
Zur Feinstruktur der Drüsen vonDrosophyllum lusitanicum
journal, April 1960
- Schnepe, Eberhard
- Planta, Vol. 54, Issue 6
Navigating the plant cell: intracellular transport logistics in the green kingdom
journal, October 2015
- Geitmann, Anja; Nebenführ, Andreas
- Molecular Biology of the Cell, Vol. 26, Issue 19
Three-Dimensional Analysis of Syncytial-Type Cell Plates during Endosperm Cellularization Visualized by High Resolution Electron Tomography
journal, September 2001
- Otegui, Marisa S.; Mastronarde, David N.; Kang, Byung-Ho
- The Plant Cell, Vol. 13, Issue 9
Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals
journal, February 2002
- Wagstaff, Carol; Leverentz, Michael K.; Griffiths, Gareth
- Journal of Experimental Botany, Vol. 53, Issue 367
Electron Tomography of RabA4b- and PI-4Kβ1-Labeled Trans Golgi Network Compartments in Arabidopsis
journal, January 2011
- Kang, Byung-Ho; Nielsen, Erik; Preuss, Mary Lai
- Traffic, Vol. 12, Issue 3
Fine Structure Changes During Function of the Digestive Gland of Venus'S-Flytrap
journal, January 1969
- Schwab, D. W.; Simmons, E.; Scala, J.
- American Journal of Botany, Vol. 56, Issue 1
The ER/Golgi Interface – Is There Anything in-between?
journal, January 2012
- Hawes, Chris
- Frontiers in Plant Science, Vol. 3
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells
journal, June 2004
- daSilva, Luis L. P.; Snapp, Erik L.; Denecke, Jürgen
- The Plant Cell, Vol. 16, Issue 7
How the Venus flytrap snaps
journal, January 2005
- Forterre, Yoël; Skotheim, Jan M.; Dumais, Jacques
- Nature, Vol. 433, Issue 7024
Protein secretion in plants
journal, April 1989
- Jones, Russell L.; Robinson, David G.
- New Phytologist, Vol. 111, Issue 4
The Secretory Cycle of Dionaea Muscipula Ellis. iii. the Mechanism of Release of Digestive Secretion
journal, November 1980
- Robins, R. J.; Juniper, B. E.
- New Phytologist, Vol. 86, Issue 3
Identification and characterization of COPIa- and COPIb-type vesicle classes associated with plant and algal Golgi
journal, December 2006
- Donohoe, B. S.; Kang, B. -H.; Staehelin, L. A.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 1
Aspartic proteinases are expressed in pitchers of the carnivorous plant Nepenthes alata Blanco
journal, March 2002
- An, Chung-Il; Fukusaki, Ei-ichiro; Kobayashi, Akio
- Planta, Vol. 214, Issue 5
Cell biology of the plant Golgi apparatus
journal, November 2004
- Hawes, Chris
- New Phytologist, Vol. 165, Issue 1
Localization of Golgi-resident glycosyltransferases
journal, September 2009
- Tu, Linna; Banfield, David Karl
- Cellular and Molecular Life Sciences, Vol. 67, Issue 1
Cis -Golgi Cisternal Assembly and Biosynthetic Activation Occur Sequentially in Plants and Algae : Cis-Golgi Cisternal Assembly and Biosynthetic Activation in Plants and Algae
journal, February 2013
- Donohoe, Bryon S.; Kang, Byung-Ho; Gerl, Mathias J.
- Traffic, Vol. 14, Issue 5
New views of cells in 3D: an introduction to electron tomography
journal, January 2005
- McIntosh, Richard; Nicastro, Daniela; Mastronarde, David
- Trends in Cell Biology, Vol. 15, Issue 1, p. 43-51
cis -Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells
journal, August 2012
- Ito, Yoko; Uemura, Tomohiro; Shoda, Keiko
- Molecular Biology of the Cell, Vol. 23, Issue 16
The Plant Golgi Apparatus: Structure, Functional Organization and Trafficking Mechanisms
journal, June 1995
- Staehelin, L. A.; Moore, I.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 46, Issue 1
A three-stage model of Golgi structure and function
journal, July 2013
- Day, Kasey J.; Staehelin, L. Andrew; Glick, Benjamin S.
- Histochemistry and Cell Biology, Vol. 140, Issue 3
Golgi compartmentation and identity
journal, August 2014
- Papanikou, Effrosyni; Glick, Benjamin S.
- Current Opinion in Cell Biology, Vol. 29
Venus flytrap carnivorous lifestyle builds on herbivore defense strategies
journal, May 2016
- Bemm, Felix; Becker, Dirk; Larisch, Christina
- Genome Research, Vol. 26, Issue 6
Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell
journal, March 1999
- Ladinsky, Mark S.; Mastronarde, David N.; McIntosh, J. Richard
- The Journal of Cell Biology, Vol. 144, Issue 6
Organization of the ER–Golgi interface for membrane traffic control
journal, May 2013
- Brandizzi, Federica; Barlowe, Charles
- Nature Reviews Molecular Cell Biology, Vol. 14, Issue 6
Dynamic Behavior of the trans-Golgi Network in Root Tissues of Arabidopsis Revealed by Super-Resolution Live Imaging
journal, February 2014
- Uemura, Tomohiro; Suda, Yasuyuki; Ueda, Takashi
- Plant and Cell Physiology, Vol. 55, Issue 4
Quantitative Zusammenhänge zwischen der Sekretion des Fangschleimes und den Golgi-Strukturen bei Drosophyllum lusitanicum
journal, September 1961
- Schnepf, Eberhard
- Zeitschrift für Naturforschung B, Vol. 16, Issue 9
Vesicles versus Tubes: Is Endoplasmic Reticulum-Golgi Transport in Plants Fundamentally Different from Other Eukaryotes?
journal, April 2015
- Robinson, David G.; Brandizzi, Federica; Hawes, Chris
- Plant Physiology, Vol. 168, Issue 2
The fine Structure of the Digestive Gland of Venus'S Flytrap
journal, July 1968
- Scala, J.; Schwab, D.; Simmons, E.
- American Journal of Botany, Vol. 55, Issue 6Part1
The Secretory Cycle of Dionaea Muscipula Ellis. i. the fine Structure and the Effect of Stimulation on the fine Structure of the Digestive Gland Cells
journal, November 1980
- Robins, R. J.; Juniper, B. E.
- New Phytologist, Vol. 86, Issue 3
Quite a few reasons for calling carnivores ‘the most wonderful plants in the world’
journal, September 2011
- Król, Elżbieta; Płachno, Bartosz J.; Adamec, Lubomír
- Annals of Botany, Vol. 109, Issue 1
ER-to-Golgi transport by COPII vesicles in Arabidopsis involves a ribosome-excluding scaffold that is transferred with the vesicles to the Golgi matrix
journal, September 2008
- Kang, Byung-Ho; Staehelin, L. Andrew
- Protoplasma, Vol. 234, Issue 1-4
Isolation and Proteomic Characterization of the Arabidopsis Golgi Defines Functional and Novel Components Involved in Plant Cell Wall Biosynthesis
journal, March 2012
- Parsons, Harriet T.; Christiansen, Katy; Knierim, Bernhard
- Plant Physiology, Vol. 159, Issue 1
Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System
journal, December 1999
- Nebenführ, Andreas; Gallagher, Larry A.; Dunahay, Terri G.
- Plant Physiology, Vol. 121, Issue 4
A cut above the rest: the regulatory function of plant proteases
journal, October 2004
- Schaller, Andreas
- Planta, Vol. 220, Issue 2
A Mobile Secretory Vesicle Cluster Involved in Mass Transport from the Golgi to the Plant Cell Exterior
journal, April 2009
- Toyooka, Kiminori; Goto, Yumi; Asatsuma, Satoru
- The Plant Cell, Vol. 21, Issue 4
De Novo Formation of Plant Endoplasmic Reticulum Export Sites Is Membrane Cargo Induced and Signal Mediated
journal, February 2007
- Hanton, Sally L.; Chatre, Laurent; Renna, Luciana
- Plant Physiology, Vol. 143, Issue 4
Insect haptoelectrical stimulation of Venus flytrap triggers exocytosis in gland cells
journal, April 2017
- Scherzer, Sönke; Shabala, Lana; Hedrich, Benjamin
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 18
Electron tomography of ER, Golgi and related membrane systems
journal, June 2006
- Donohoe, Bryon S.; Mogelsvang, Soren; Staehelin, L. Andrew
- Methods, Vol. 39, Issue 2
The Secretory Cycle of Dionaea Muscipula Ellis.. ii Storage and Synthesis of the Secretory Proteins
journal, November 1980
- Robins, R. J.; Juniper, B. E.
- New Phytologist, Vol. 86, Issue 3
The Fine Structure of the Digestive Gland of Venus's Flytrap
journal, July 1968
- Scala, J.; Schwab, D.; Simmons, E.
- American Journal of Botany, Vol. 55, Issue 6
Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases
journal, September 2004
- Athauda, S. P. B.; Matsumoto, K.; Rajapakshe, S.
- Biochemical Journal, Vol. 382, Issue 3
Gibberellic acid and abscisic acid modulate protein synthesis and mRNA levels in barley aleurone layers
journal, January 1982
- Higgins, Thomas J. V.; Jacobsen, John V.; Zwar, John A.
- Plant Molecular Biology, Vol. 1, Issue 3
Structural organization of ultrarapidly frozen barley aleurone cells actively involved in protein secretion
journal, January 1985
- Fernandez, D. E.; Staehelin, L. A.
- Planta, Vol. 165, Issue 4
Macromolecular differentiation of Golgi stacks in root tips ofArabidopsis andNicotiana seedlings as visualized in high pressure frozen and freeze-substituted samples
journal, February 1990
- Staehelin, L. A.; Giddings, T. H.; Kiss, J. Z.
- Protoplasma, Vol. 157, Issue 1-3
Golgi apparatus and slime secretion in plants: the early implications and recent models of membrane traffic
journal, March 1993
- Schnepf, E.
- Protoplasma, Vol. 172, Issue 1
Localization of Golgi-resident glycosyltransferases
journal, September 2009
- Tu, Linna; Banfield, David Karl
- Cellular and Molecular Life Sciences, Vol. 67, Issue 1
A cut above the rest: the regulatory function of plant proteases
journal, October 2004
- Schaller, Andreas
- Planta, Vol. 220, Issue 2
Aspartic proteinases are expressed in pitchers of the carnivorous plant Nepenthes alata Blanco
journal, March 2002
- An, Chung-Il; Fukusaki, Ei-ichiro; Kobayashi, Akio
- Planta, Vol. 214, Issue 5
ER-to-Golgi transport by COPII vesicles in Arabidopsis involves a ribosome-excluding scaffold that is transferred with the vesicles to the Golgi matrix
journal, September 2008
- Kang, Byung-Ho; Staehelin, L. Andrew
- Protoplasma, Vol. 234, Issue 1-4
Golgi compartmentation and identity
journal, August 2014
- Papanikou, Effrosyni; Glick, Benjamin S.
- Current Opinion in Cell Biology, Vol. 29
INF2-Mediated Severing through Actin Filament Encirclement and Disruption
journal, January 2014
- Gurel, Pinar S.; Ge, Peng; Grintsevich, Elena E.
- Current Biology, Vol. 24, Issue 2
The Venus Flytrap Dionaea muscipula Counts Prey-Induced Action Potentials to Induce Sodium Uptake
journal, February 2016
- Böhm, Jennifer; Scherzer, Sönke; Krol, Elzbieta
- Current Biology, Vol. 26, Issue 3
New views of cells in 3D: an introduction to electron tomography
journal, January 2005
- McIntosh, Richard; Nicastro, Daniela; Mastronarde, David
- Trends in Cell Biology, Vol. 15, Issue 1, p. 43-51
Electron tomography of ER, Golgi and related membrane systems
journal, June 2006
- Donohoe, Bryon S.; Mogelsvang, Soren; Staehelin, L. Andrew
- Methods, Vol. 39, Issue 2
How the Venus flytrap snaps
journal, January 2005
- Forterre, Yoël; Skotheim, Jan M.; Dumais, Jacques
- Nature, Vol. 433, Issue 7024
Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases
journal, June 2004
- Athauda, Senarath B. P.; Matsumoto, Koji; Rajapakshe, Sanath
- Biochemical Journal, Vol. 381, Issue 1
Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network †
journal, August 1998
- Boevink, Petra; Oparka, Karl; Cruz, Simon Santa
- The Plant Journal, Vol. 15, Issue 3
Identification and characterization of COPIa- and COPIb-type vesicle classes associated with plant and algal Golgi
journal, December 2006
- Donohoe, B. S.; Kang, B. -H.; Staehelin, L. A.
- Proceedings of the National Academy of Sciences, Vol. 104, Issue 1
Insect haptoelectrical stimulation of Venus flytrap triggers exocytosis in gland cells
journal, April 2017
- Scherzer, Sönke; Shabala, Lana; Hedrich, Benjamin
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 18
The Protein Composition of the Digestive Fluid from the Venus Flytrap Sheds Light on Prey Digestion Mechanisms
journal, August 2012
- Schulze, Waltraud X.; Sanggaard, Kristian W.; Kreuzer, Ines
- Molecular & Cellular Proteomics, Vol. 11, Issue 11
Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell
journal, March 1999
- Ladinsky, Mark S.; Mastronarde, David N.; McIntosh, J. Richard
- The Journal of Cell Biology, Vol. 144, Issue 6
cis -Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells
journal, August 2012
- Ito, Yoko; Uemura, Tomohiro; Shoda, Keiko
- Molecular Biology of the Cell, Vol. 23, Issue 16
Navigating the plant cell: intracellular transport logistics in the green kingdom
journal, October 2015
- Geitmann, Anja; Nebenführ, Andreas
- Molecular Biology of the Cell, Vol. 26, Issue 19
Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals
journal, February 2002
- Wagstaff, Carol; Leverentz, Michael K.; Griffiths, Gareth
- Journal of Experimental Botany, Vol. 53, Issue 367
Mechano-Stimulation Triggers Turgor Changes Associated with Trap Closure in the Darwin Plant Dionaea muscipula
journal, April 2014
- Escalante-Pérez, María; Scherzer, Sönke; Al-Rasheid, Khaled A. S.
- Molecular Plant, Vol. 7, Issue 4
Dynamic Behavior of the trans-Golgi Network in Root Tissues of Arabidopsis Revealed by Super-Resolution Live Imaging
journal, February 2014
- Uemura, Tomohiro; Suda, Yasuyuki; Ueda, Takashi
- Plant and Cell Physiology, Vol. 55, Issue 4
Venus flytrap carnivorous lifestyle builds on herbivore defense strategies
journal, May 2016
- Bemm, Felix; Becker, Dirk; Larisch, Christina
- Genome Research, Vol. 26, Issue 6
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and of Golgi Membranes as Determined by Electron Tomography
journal, August 2008
- Staehelin, L. Andrew; Kang, Byung-Ho
- Plant Physiology, Vol. 147, Issue 4
Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System
journal, December 1999
- Nebenführ, Andreas; Gallagher, Larry A.; Dunahay, Terri G.
- Plant Physiology, Vol. 121, Issue 4
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells
journal, June 2004
- daSilva, Luis L. P.; Snapp, Erik L.; Denecke, Jürgen
- The Plant Cell, Vol. 16, Issue 7
Dynamics of COPII Vesicles and the Golgi Apparatus in Cultured Nicotiana tabacum BY-2 Cells Provides Evidence for Transient Association of Golgi Stacks with Endoplasmic Reticulum Exit Sites
journal, April 2005
- Yang, Yao-dong; Elamawi, Rabab; Bubeck, Julia
- The Plant Cell, Vol. 17, Issue 5
Analysis of the Golgi Apparatus in Arabidopsis Seed Coat Cells during Polarized Secretion of Pectin-Rich Mucilage
journal, June 2008
- Young, Robin E.; McFarlane, Heather E.; Hahn, Michael G.
- The Plant Cell, Vol. 20, Issue 6
A Mobile Secretory Vesicle Cluster Involved in Mass Transport from the Golgi to the Plant Cell Exterior
journal, April 2009
- Toyooka, Kiminori; Goto, Yumi; Asatsuma, Satoru
- The Plant Cell, Vol. 21, Issue 4
The Secretory Cycle of Dionaea Muscipula Ellis. i. the fine Structure and the Effect of Stimulation on the fine Structure of the Digestive Gland Cells
journal, November 1980
- Robins, R. J.; Juniper, B. E.
- New Phytologist, Vol. 86, Issue 3
The Secretory Cycle of Dionaea Muscipula Ellis. iii. the Mechanism of Release of Digestive Secretion
journal, November 1980
- Robins, R. J.; Juniper, B. E.
- New Phytologist, Vol. 86, Issue 3
Cell biology of the plant Golgi apparatus
journal, November 2004
- Hawes, Chris
- New Phytologist, Vol. 165, Issue 1
Electron Tomography of RabA4b- and PI-4Kβ1-Labeled Trans Golgi Network Compartments in Arabidopsis
journal, January 2011
- Kang, Byung-Ho; Nielsen, Erik; Preuss, Mary Lai
- Traffic, Vol. 12, Issue 3
A distinct class of vesicles derived from the trans -Golgi mediates secretion of xylogalacturonan in the root border cell
journal, October 2017
- Wang, Pengfei; Chen, Xinshi; Goldbeck, Cameron
- The Plant Journal, Vol. 92, Issue 4
Membrane Traffic Within the Golgi Apparatus
journal, November 2009
- Glick, Benjamin S.; Nakano, Akihiko
- Annual Review of Cell and Developmental Biology, Vol. 25, Issue 1
Acid phosphatase localization in the digestive glands of Dionaea muscipula Ellis flytraps.
journal, April 1985
- Henry, Y.; Steer, M. W.
- Journal of Histochemistry & Cytochemistry, Vol. 33, Issue 4
Quantitative Zusammenhänge zwischen der Sekretion des Fangschleimes und den Golgi-Strukturen bei Drosophyllum lusitanicum
journal, September 1961
- Schnepf, Eberhard
- Zeitschrift für Naturforschung B, Vol. 16, Issue 9
The Fine Structure of the Digestive Gland of Venus's Flytrap
journal, July 1968
- Scala, J.; Schwab, D.; Simmons, E.
- American Journal of Botany, Vol. 55, Issue 6
The ER/Golgi Interface – Is There Anything in-between?
journal, January 2012
- Hawes, Chris
- Frontiers in Plant Science, Vol. 3