Title: Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids

Abstract

Contents The use of artificial insemination in cattle breeding has evolved to global extent, and insemination doses are often shipped via air transport which requires strict radiation‐based examinations. For the determination of effect of non‐ionizing radiation (NIR), to which are beings frequently exposed due to protection of airport or cultural event security, freshly ejaculated and cryopreserved bovine spermatozoa were used as experimental model. Following radiation with hand‐held metal detector in various exposition times (0, 10 s, 15, 30 and 60 min—groups FR, FR10, FR15, FR30 and FR60) the spermatozoa underwent motility and DNA fragmentation analyses. Study on cryoconserved semen treated with NIR was performed in time intervals 0, 10 s, 1 and 5 min (insemination doses radiated before cryoconservation—CB, CB10, CB1, CB5; samples radiated after freezing—CA, CA10, CA1 and CA5). Fresh semen and insemination doses radiated after cryoconservation showed significantly lower total and progressive motility. No effect on motility parameters was detected in semen extended with cryopreservative medium and radiated prior to freezing. Surprisingly, NIR showed a potential to stimulate spermatozoa velocity; however, the effect was modulated throughout the post‐thawing incubation. Based on the DNA fragmentation assay, sperm DNA stayed intact. Present study underlines the potential harm of NIR, which is frequently used inmore » everyday life, with overall adverse impact on human and animal reproduction. Current study also points out on interesting short‐term spermatozoa stimulation induced by NIR.« less

Authors:

Tirpak, Filip  ^[1];

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• Search DOE PAGES for ORCID "0000-0001-9293-9055"

Slanina, Tomas  ^[1];

• Search DOE PAGES for author "Slanina, Tomas"
• Search DOE PAGES for ORCID "0000-0001-8524-2620"

Tomka, Marian ^[2]; Zidek, Radoslav ^[3]; Halo, Jr, Marko ^[1]; Ivanic, Peter ^[4]; Gren, Agnieszka ^[5]; Formicki, Grzegorz ^[5]; Stachanczyk, Katarzyna ^[5]; Lukac, Norbert ^[1]; Massanyi, Peter ^[6]

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+ Show Author Affiliations

1. Department of Animal Physiology, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic
2. Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic
3. Department of Hygiene and Food Safety, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic
4. Slovak Biological Services Banska Bystrica Slovak Republic
5. Institute of Biology Pedagogical University of Krakow Krakow Poland
6. Department of Animal Physiology, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic, Institute of Biology Pedagogical University of Krakow Krakow Poland

Publication Date:

Sat Sep 29 00:00:00 EDT 2018

Sponsoring Org.:

USDOE

OSTI Identifier:

1475073

Resource Type:

Publisher's Accepted Manuscript

Journal Name:

Reproduction in Domestic Animals

Additional Journal Information:

Journal Name: Reproduction in Domestic Animals Journal Volume: 54 Journal Issue: 2; Journal ID: ISSN 0936-6768

Publisher:

Wiley-Blackwell

Country of Publication:

Country unknown/Code not available

Language:

English