Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids
Abstract
Contents The use of artificial insemination in cattle breeding has evolved to global extent, and insemination doses are often shipped via air transport which requires strict radiation‐based examinations. For the determination of effect of non‐ionizing radiation (NIR), to which are beings frequently exposed due to protection of airport or cultural event security, freshly ejaculated and cryopreserved bovine spermatozoa were used as experimental model. Following radiation with hand‐held metal detector in various exposition times (0, 10 s, 15, 30 and 60 min—groups FR, FR10, FR15, FR30 and FR60) the spermatozoa underwent motility and DNA fragmentation analyses. Study on cryoconserved semen treated with NIR was performed in time intervals 0, 10 s, 1 and 5 min (insemination doses radiated before cryoconservation—CB, CB10, CB1, CB5; samples radiated after freezing—CA, CA10, CA1 and CA5). Fresh semen and insemination doses radiated after cryoconservation showed significantly lower total and progressive motility. No effect on motility parameters was detected in semen extended with cryopreservative medium and radiated prior to freezing. Surprisingly, NIR showed a potential to stimulate spermatozoa velocity; however, the effect was modulated throughout the post‐thawing incubation. Based on the DNA fragmentation assay, sperm DNA stayed intact. Present study underlines the potential harm of NIR, which is frequently used inmore »
- Authors:
-
- Department of Animal Physiology, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic
- Department of Biochemistry and Biotechnology, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic
- Department of Hygiene and Food Safety, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic
- Slovak Biological Services Banska Bystrica Slovak Republic
- Institute of Biology Pedagogical University of Krakow Krakow Poland
- Department of Animal Physiology, Faculty of Biotechnology and Food Sciences Slovak University of Agriculture in Nitra Nitra Slovak Republic, Institute of Biology Pedagogical University of Krakow Krakow Poland
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1475073
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Reproduction in Domestic Animals
- Additional Journal Information:
- Journal Name: Reproduction in Domestic Animals Journal Volume: 54 Journal Issue: 2; Journal ID: ISSN 0936-6768
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Citation Formats
Tirpak, Filip, Slanina, Tomas, Tomka, Marian, Zidek, Radoslav, Halo, Jr, Marko, Ivanic, Peter, Gren, Agnieszka, Formicki, Grzegorz, Stachanczyk, Katarzyna, Lukac, Norbert, and Massanyi, Peter. Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids. Country unknown/Code not available: N. p., 2018.
Web. doi:10.1111/rda.13338.
Tirpak, Filip, Slanina, Tomas, Tomka, Marian, Zidek, Radoslav, Halo, Jr, Marko, Ivanic, Peter, Gren, Agnieszka, Formicki, Grzegorz, Stachanczyk, Katarzyna, Lukac, Norbert, & Massanyi, Peter. Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids. Country unknown/Code not available. https://doi.org/10.1111/rda.13338
Tirpak, Filip, Slanina, Tomas, Tomka, Marian, Zidek, Radoslav, Halo, Jr, Marko, Ivanic, Peter, Gren, Agnieszka, Formicki, Grzegorz, Stachanczyk, Katarzyna, Lukac, Norbert, and Massanyi, Peter. Sat .
"Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids". Country unknown/Code not available. https://doi.org/10.1111/rda.13338.
@article{osti_1475073,
title = {Exposure to non‐ionizing electromagnetic radiation of public risk prevention instruments threatens the quality of spermatozoids},
author = {Tirpak, Filip and Slanina, Tomas and Tomka, Marian and Zidek, Radoslav and Halo, Jr, Marko and Ivanic, Peter and Gren, Agnieszka and Formicki, Grzegorz and Stachanczyk, Katarzyna and Lukac, Norbert and Massanyi, Peter},
abstractNote = {Contents The use of artificial insemination in cattle breeding has evolved to global extent, and insemination doses are often shipped via air transport which requires strict radiation‐based examinations. For the determination of effect of non‐ionizing radiation (NIR), to which are beings frequently exposed due to protection of airport or cultural event security, freshly ejaculated and cryopreserved bovine spermatozoa were used as experimental model. Following radiation with hand‐held metal detector in various exposition times (0, 10 s, 15, 30 and 60 min—groups FR, FR10, FR15, FR30 and FR60) the spermatozoa underwent motility and DNA fragmentation analyses. Study on cryoconserved semen treated with NIR was performed in time intervals 0, 10 s, 1 and 5 min (insemination doses radiated before cryoconservation—CB, CB10, CB1, CB5; samples radiated after freezing—CA, CA10, CA1 and CA5). Fresh semen and insemination doses radiated after cryoconservation showed significantly lower total and progressive motility. No effect on motility parameters was detected in semen extended with cryopreservative medium and radiated prior to freezing. Surprisingly, NIR showed a potential to stimulate spermatozoa velocity; however, the effect was modulated throughout the post‐thawing incubation. Based on the DNA fragmentation assay, sperm DNA stayed intact. Present study underlines the potential harm of NIR, which is frequently used in everyday life, with overall adverse impact on human and animal reproduction. Current study also points out on interesting short‐term spermatozoa stimulation induced by NIR.},
doi = {10.1111/rda.13338},
journal = {Reproduction in Domestic Animals},
number = 2,
volume = 54,
place = {Country unknown/Code not available},
year = {Sat Sep 29 00:00:00 EDT 2018},
month = {Sat Sep 29 00:00:00 EDT 2018}
}
https://doi.org/10.1111/rda.13338
Web of Science
Works referenced in this record:
Non-ionizing radiation (NIR): Evaluating safety
journal, March 2017
- Blank, Martin
- Environmental Pollution, Vol. 222
Effects of airport screening X-irradiation on bovine sperm chromatin integrity and embryo development
journal, January 2010
- Hendricks, K. E. M.; Penfold, L. M.; Evenson, D. P.
- Theriogenology, Vol. 73, Issue 2
Caffeine strongly improves motility parameters of turkey spermatozoa with no effect on cell viability
journal, March 2018
- Slanina, Tomáš; Miškeje, Michal; Tirpák, Filip
- Acta Veterinaria Hungarica, Vol. 66, Issue 1
Effects of 50Hz extremely low frequency magnetic field on the morphology and function of boar spermatozoa capacitated in vitro
journal, March 2007
- Bernabò, N.; Tettamanti, E.; Pistilli, M. G.
- Theriogenology, Vol. 67, Issue 4
DNA fragmentation of human sperm can be detected by ligation-mediated real-time polymerase chain reaction
journal, December 2013
- Lim, Jung Jin; Lee, Jin Il; Kim, Dong Hwan
- Fertility and Sterility, Vol. 100, Issue 6
Exposure to electromagnetic fields (non-ionizing radiation) and its relationship with childhood leukemia: A systematic review
journal, July 2010
- Calvente, I.; Fernandez, M. F.; Villalba, J.
- Science of The Total Environment, Vol. 408, Issue 16
When theory and observation collide: Can non-ionizing radiation cause cancer?
journal, February 2017
- Havas, Magda
- Environmental Pollution, Vol. 221
A preliminary study of oscillating electromagnetic field effects on human spermatozoon motility
journal, January 2006
- Iorio, R.; Scrimaglio, R.; Rantucci, E.
- Bioelectromagnetics, Vol. 28, Issue 1
The Effect of HT-2 Toxin on Ovarian Steroidogenesis and Its Response to IGF-I, Leptin and Ghrelin in Rabbits
journal, August 2017
- Kolesarova, A.; Maruniakova, N.; Kadasi, A.
- Physiological Research
Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study
journal, October 2009
- Agarwal, Ashok; Desai, Nisarg R.; Makker, Kartikeya
- Fertility and Sterility, Vol. 92, Issue 4
No induction of chromosome aberrations in human spermatozoa exposed to extremely low frequency electromagnetic fields
journal, May 1998
- Tateno, Hiroyuki; Iijima, Sumio; Nakanishi, Yoshifumi
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 414, Issue 1-3
Biological and health effects of radiofrequency fields: Good study design and quality publications
journal, November 2016
- Vijayalaxmi,
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 810
Assessment of a potential preventive ability of amygdalin in mycotoxin-induced ovarian toxicity
journal, April 2015
- Halenar, Marek; Medvedova, Marina; Maruniakova, Nora
- Journal of Environmental Science and Health, Part B, Vol. 50, Issue 6
Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline
journal, June 2005
- Aitken, R. J.; Bennetts, L. E.; Sawyer, D.
- International Journal of Andrology, Vol. 28, Issue 3
Analysis of DNA Fragmentation Using Agarose Gel Electrophoresis
journal, January 2006
- Kasibhatla, Shailaja; Amarante-Mendes, Gustavo P.; Finucane, Deborah
- Cold Spring Harbor Protocols, Vol. 2006, Issue 1
Conservation Science in a Terrorist age: the Impact of Airport Security Screening on the Viability and dna Integrity of Frozen Felid Spermatozoa
journal, September 2006
- Gloor, Kayleen T.; Winget, Doug; Swanson, William F.
- Journal of Zoo and Wildlife Medicine, Vol. 37, Issue 3
In vitro effect of 4-nonylphenol on human chorionic gonadotropin (hCG) stimulated hormone secretion, cell viability and reactive oxygen species generation in mice Leydig cells
journal, March 2017
- Jambor, Tomáš; Tvrdá, Eva; Tušimová, Eva
- Environmental Pollution, Vol. 222
Best Biomarker and Bioindicator for Marine Environmental Pollution
journal, January 2012
- Tosti, Elisabetta
- Journal of Marine Science: Research & Development, Vol. 02, Issue 02
Involvement of mitochondrial activity in mediating ELF-EMF stimulatory effect on human sperm motility
journal, August 2010
- Iorio, Roberto; Delle Monache, Simona; Bennato, Francesca
- Bioelectromagnetics, Vol. 32, Issue 1
Adaptive response in mammalian cells exposed to non-ionizing radiofrequency fields: A review and gaps in knowledge
journal, April 2014
- Vijayalaxmi, ; Cao, Yi; Scarfi, Maria Rosaria
- Mutation Research/Reviews in Mutation Research, Vol. 760
Electromagnetic acceleration of electron transfer reactions
journal, January 2001
- Blank, Martin; Soo, Lily
- Journal of Cellular Biochemistry, Vol. 81, Issue 2
The link between radiofrequencies emitted from wireless technologies and oxidative stress
journal, September 2016
- Dasdag, Suleyman; Akdag, Mehmet Zulkuf
- Journal of Chemical Neuroanatomy, Vol. 75
Influence of a 50 Hz extra low frequency electromagnetic field on spermatozoa motility and fertilization rates in rabbits
journal, August 2009
- Roychoudhury, Shubhadeep; Jedlicka, Jaroslav; Parkanyi, Vladimir
- Journal of Environmental Science and Health, Part A, Vol. 44, Issue 10
In vitro copper inhibition of the rabbit spermatozoa motility
journal, April 2008
- Roychoudhury, Shubhadeep; Massanyi, Peter
- Journal of Environmental Science and Health, Part A, Vol. 43, Issue 6
Effect of long-term exposure of 2.4 GHz radiofrequency radiation emitted from Wi-Fi equipment on testes functions
journal, November 2013
- Dasdag, Suleyman; Taş, Muzaffer; Akdag, Mehmet Zulkuf
- Electromagnetic Biology and Medicine, Vol. 34, Issue 1
Effect of electromagnetic field exposure on the reproductive system
journal, January 2012
- Gye, Myung Chan; Park, Chan Jin
- Clinical and Experimental Reproductive Medicine, Vol. 39, Issue 1
Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility [Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility]
journal, March 2018
- Slanina, T.; Miškeje, M.; Tirpák, F.
- Czech Journal of Animal Science, Vol. 63, Issue No. 4
Changes in Cellular Proteins due to Environmental Non-Ionizing Radiation. i. Heat-Shock Proteins
journal, January 2001
- Kwee, S.; Raskmark, P.; Velizarov, S.
- Electro- and Magnetobiology, Vol. 20, Issue 2