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Title: Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ

Abstract

Bacteria capable of reduction of nitrous oxide (N2O) to N2 separate into clade I and clade II organisms on the basis of nos operon structures and nosZ sequence features. In this paper, to explore the possible ecological consequences of distinct nos clusters, the growth of bacterial isolates with either clade I (Pseudomonas stutzeri strain DCP-Ps1, Shewanella loihica strain PV-4) or clade II (Dechloromonas aromatica strain RCB, Anaeromyxobacter dehalogenans strain 2CP-C) nosZ with N2O was examined. Growth curves did not reveal trends distinguishing the clade I and clade II organisms tested; however, the growth yields of clade II organisms exceeded those of clade I organisms by 1.5- to 1.8-fold. Further, whole-cell half-saturation constants (Ks s) for N2O distinguished clade I from clade II organisms. The apparent Ks values of 0.324 ± 0.078 μM for D. aromatica and 1.34 ± 0.35 μM for A. dehalogenans were significantly lower than the values measured for P. stutzeri (35.5 ± 9.3 μM) and S. loihica (7.07 ± 1.13 μM). Genome sequencing demonstrated that Dechloromonas denitrificans possessed a clade II nosZ gene, and a measured Ks of 1.01 ± 0.18 μM for N2O was consistent with the values determined for the other clade II organisms tested.more » Finally, these observations provide a plausible mechanistic basis for why the relative activity of bacteria with clade I nos operons compared to that of bacteria with clade II nos operons may control N2O emissions and determine a soil's N2O sink capacity.« less

Authors:
 [1];  [2];  [3];  [4];  [5]
  1. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology. Dept. of Microbiology; Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea, Republic of). Dept. of Civil and Environmental Engineering
  2. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). UT-ORNL Joint Inst. for Biological Sciences (JIBS). Biosciences Division
  3. Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea, Republic of). Dept. of Civil and Environmental Engineering
  4. Univ. of Illinois, Urbana, IL (United States). Dept. of Geology
  5. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology. Dept. of Microbiology. Dept. of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). UT-ORNL Joint Inst. for Biological Sciences (JIBS). Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States); Korea Advanced Inst. of Science and Technology (KAIST), Daejeon (Korea, Republic of)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Research Foundation of Korea (NRF)
OSTI Identifier:
1474731
Grant/Contract Number:  
AC05-00OR22725; SC0006662; 2014R1A1A2058543
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 82; Journal Issue: 13; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Yoon, Sukhwan, Nissen, Silke, Park, Doyoung, Sanford, Robert A., and Löffler, Frank E. Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ. United States: N. p., 2016. Web. doi:10.1128/AEM.00409-16.
Yoon, Sukhwan, Nissen, Silke, Park, Doyoung, Sanford, Robert A., & Löffler, Frank E. Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ. United States. doi:10.1128/AEM.00409-16.
Yoon, Sukhwan, Nissen, Silke, Park, Doyoung, Sanford, Robert A., and Löffler, Frank E. Fri . "Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ". United States. doi:10.1128/AEM.00409-16. https://www.osti.gov/servlets/purl/1474731.
@article{osti_1474731,
title = {Nitrous Oxide Reduction Kinetics Distinguish Bacteria Harboring Clade I NosZ from Those Harboring Clade II NosZ},
author = {Yoon, Sukhwan and Nissen, Silke and Park, Doyoung and Sanford, Robert A. and Löffler, Frank E.},
abstractNote = {Bacteria capable of reduction of nitrous oxide (N2O) to N2 separate into clade I and clade II organisms on the basis of nos operon structures and nosZ sequence features. In this paper, to explore the possible ecological consequences of distinct nos clusters, the growth of bacterial isolates with either clade I (Pseudomonas stutzeri strain DCP-Ps1, Shewanella loihica strain PV-4) or clade II (Dechloromonas aromatica strain RCB, Anaeromyxobacter dehalogenans strain 2CP-C) nosZ with N2O was examined. Growth curves did not reveal trends distinguishing the clade I and clade II organisms tested; however, the growth yields of clade II organisms exceeded those of clade I organisms by 1.5- to 1.8-fold. Further, whole-cell half-saturation constants (Ks s) for N2O distinguished clade I from clade II organisms. The apparent Ks values of 0.324 ± 0.078 μM for D. aromatica and 1.34 ± 0.35 μM for A. dehalogenans were significantly lower than the values measured for P. stutzeri (35.5 ± 9.3 μM) and S. loihica (7.07 ± 1.13 μM). Genome sequencing demonstrated that Dechloromonas denitrificans possessed a clade II nosZ gene, and a measured Ks of 1.01 ± 0.18 μM for N2O was consistent with the values determined for the other clade II organisms tested. Finally, these observations provide a plausible mechanistic basis for why the relative activity of bacteria with clade I nos operons compared to that of bacteria with clade II nos operons may control N2O emissions and determine a soil's N2O sink capacity.},
doi = {10.1128/AEM.00409-16},
journal = {Applied and Environmental Microbiology},
number = 13,
volume = 82,
place = {United States},
year = {2016},
month = {4}
}

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