Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction

Conway, Jonathan M.; McKinley, Bennett S.; Seals, Nathaniel L.; Hernandez, Diana H.; Khatibi, Piyum A.; Poudel, Suresh; Giannone, Richard J.; Hettich, Robert L.; Williams-Rhaesa, Amanda M.; Lipscomb, Gina L.; Adams, Michael W. W.; Kelly, Robert M.

doi:10.1128/AEM.01828-17

Title: Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction

Journal Article · 06 October 2017 · Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/AEM.01828-17 · OSTI ID:1474620

Conway, Jonathan M. ^[1]; McKinley, Bennett S. ^[1]; Seals, Nathaniel L. ^[1]; Hernandez, Diana H. ^[1]; Khatibi, Piyum A. ^[1]; Poudel, Suresh ^[2];

^[2];

^[2]; Williams-Rhaesa, Amanda M. ^[3]; Lipscomb, Gina L. ^[3]; Adams, Michael W. W. ^[3]; Kelly, Robert M. ^[1]

North Carolina State Univ., Raleigh, NC (United States). Department of Chemical and Biomolecular Engineering
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Univ. of Georgia, Athens, GA (United States). Department of Biochemistry and Molecular Biology

We present that the ability to hydrolyze microcrystalline cellulose is an uncommon feature in the microbial world, but it can be exploited for conversion of lignocellulosic feedstocks into biobased fuels and chemicals. Understanding the physiological and biochemical mechanisms by which microorganisms deconstruct cellulosic material is key to achieving this objective. The glucan degradation locus (GDL) in the genomes of extremely thermophilic Caldicellulosiruptor species encodes polysaccharide lyases (PLs), unique cellulose binding proteins (tapirins), and putative posttranslational modifying enzymes, in addition to multidomain, multifunctional glycoside hydrolases (GHs), thereby representing an alternative paradigm for plant biomass degradation compared to fungal or cellulosomal systems. To examine the individual and collective in vivoroles of the glycolytic enzymes, the six GH genes in the GDL of Caldicellulosiruptor bescii were systematically deleted, and the extents to which the resulting mutant strains could solubilize microcrystalline cellulose (Avicel) and plant biomass (switchgrass or poplar) were examined. Three of the GDL enzymes, Athe_1867 (CelA) (GH9-CBM3-CBM3-CBM3-GH48), Athe_1859 (GH5-CBM3-CBM3-GH44), and Athe_1857 (GH10-CBM3-CBM3-GH48), acted synergisticallyin vivoand accounted for 92% of naked microcrystalline cellulose (Avicel) degradation. However, the relative importance of the GDL GHs varied for the plant biomass substrates tested. Furthermore, mixed cultures of mutant strains showed that switchgrass solubilization depended on the secretome-bound enzymes collectively produced by the culture, not on the specific strain from which they came. Lastly, these results demonstrate that certain GDL GHs are primarily responsible for the degradation of microcrystalline cellulose-containing substrates by C. bescii and provide new insights into the workings of a novel microbial mechanism for lignocellulose utilization.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1474620

Journal Information:: Applied and Environmental Microbiology, Vol. 83, Issue 24; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 28 works

Citation information provided by
Web of Science

References (60)

The catalytic mechanism and unique low pH optimum of Caldicellulosiruptor bescii family 3 pectate lyase Alahuhta, Markus; Taylor, Larry E.; Brunecky, Roman Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 9 https://doi.org/10.1107/S1399004715013760	journal	August 2015
Extracellular Secretion of Noncatalytic Plant Cell Wall-Binding Proteins by the Cellulolytic Thermophile Caldicellulosiruptor bescii Yokoyama, H.; Yamashita, T.; Morioka, R. Journal of Bacteriology, Vol. 196, Issue 21 https://doi.org/10.1128/JB.01897-14	journal	August 2014
S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus Ozdemir, Inci; Blumer-Schuette, Sara E.; Kelly, Robert M. Applied and Environmental Microbiology, Vol. 78, Issue 3 https://doi.org/10.1128/AEM.07031-11	journal	December 2011
A Highly Thermostable Kanamycin Resistance Marker Expands the Tool Kit for Genetic Manipulation of Caldicellulosiruptor bescii Lipscomb, Gina L.; Conway, Jonathan M.; Blumer-Schuette, Sara E. Applied and Environmental Microbiology, Vol. 82, Issue 14 https://doi.org/10.1128/AEM.00570-16	journal	May 2016
Classification of 'Anaerocellum thermophilum' strain DSM 6725 as Caldicellulosiruptor bescii sp. nov. Yang, S. -J.; Kataeva, I.; Wiegel, J. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 9 https://doi.org/10.1099/ijs.0.017731-0	journal	October 2009
Natural genetic variability reduces recalcitrance in poplar Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0521-2	journal	May 2016
The cellulolytic system of Clostridium cellulolyticum Bélaich, Jean-pierre; Tardif, Chantal; Bélaich, Anne Journal of Biotechnology, Vol. 57, Issue 1-3 https://doi.org/10.1016/S0168-1656(97)00085-0	journal	September 1997
Degradation of microcrystalline cellulose and non-pretreated plant biomass by a cell-free extracellular cellulase/hemicellulase system from the extreme thermophilic bacterium Caldicellulosiruptor bescii Kanafusa-Shinkai, Sumiyo; Wakayama, Jun'ichi; Tsukamoto, Kazumi Journal of Bioscience and Bioengineering, Vol. 115, Issue 1 https://doi.org/10.1016/j.jbiosc.2012.07.019	journal	January 2013
Protein O-mannosylation: Conserved from bacteria to humans Lommel, M.; Strahl, S. Glycobiology, Vol. 19, Issue 8 https://doi.org/10.1093/glycob/cwp066	journal	May 2009
PAV1, the First Virus-Like Particle Isolated from a Hyperthermophilic Euryarchaeote, "Pyrococcus abyssi" Geslin, C.; Le Romancer, M.; Erauso, G. Journal of Bacteriology, Vol. 185, Issue 13 https://doi.org/10.1128/JB.185.13.3888-3894.2003	journal	July 2003
Real-Time DNA Sequencing from Single Polymerase Molecules Eid, John; Fehr, Adrian; Gray, Jeremy Science, Vol. 323, Issue 5910, p. 133-138 https://doi.org/10.1126/science.1162986	journal	January 2009
Deletion of a gene cluster encoding pectin degrading enzymes in Caldicellulosiruptor bescii reveals an important role for pectin in plant biomass recalcitrance Chung, Daehwan; Pattathil, Sivakumar; Biswal, Ajaya K. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0147-1	journal	October 2014
Pour some sugar on it: the expanding world of bacterial protein O -linked glycosylation : Protein Iwashkiw, Jeremy A.; Vozza, Nicolas F.; Kinsella, Rachel L. Molecular Microbiology, Vol. 89, Issue 1 https://doi.org/10.1111/mmi.12265	journal	June 2013
Discrete and Structurally Unique Proteins (Tāpirins) Mediate Attachment of Extremely Thermophilic Caldicellulosiruptor Species to Cellulose Blumer-Schuette, Sara E.; Alahuhta, Markus; Conway, Jonathan M. Journal of Biological Chemistry, Vol. 290, Issue 17 https://doi.org/10.1074/jbc.M115.641480	journal	February 2015
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/β-Glucanase That Can Degrade Crystalline Cellulose Xue, Xianli; Wang, Rong; Tu, Tao Applied and Environmental Microbiology, Vol. 81, Issue 11 https://doi.org/10.1128/AEM.00432-15	journal	March 2015
Cellulase and hemicellulase genes of Clostridium thermocellum from five independent collections contain few overlaps and are widely scattered across the chromosome Guglielmi, GÃ©rard; BÃ©guin, Pierre FEMS Microbiology Letters, Vol. 161, Issue 1 https://doi.org/10.1111/j.1574-6968.1998.tb12950.x	journal	April 1998
Hemicelluloses for fuel ethanol: A review Gírio, F. M.; Fonseca, C.; Carvalheiro, F. Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2010.01.088	journal	July 2010
Multidomain, Surface Layer-associated Glycoside Hydrolases Contribute to Plant Polysaccharide Degradation by Caldicellulosiruptor Species Conway, Jonathan M.; Pierce, William S.; Le, Jaycee H. Journal of Biological Chemistry, Vol. 291, Issue 13 https://doi.org/10.1074/jbc.M115.707810	journal	January 2016
Thermophilic lignocellulose deconstruction Blumer-Schuette, Sara E.; Brown, Steven D.; Sander, Kyle B. FEMS Microbiology Reviews, Vol. 38, Issue 3 https://doi.org/10.1111/1574-6976.12044	journal	May 2014
Clostridium cellulolyticum : model organism of mesophilic cellulolytic clostridia Desvaux, Mickaël FEMS Microbiology Reviews, Vol. 29, Issue 4 https://doi.org/10.1016/j.femsre.2004.11.003	journal	September 2005
Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases Lipscomb, Gina L.; Stirrett, Karen; Schut, Gerrit J. Applied and Environmental Microbiology, Vol. 77, Issue 7, p. 2232-2238 https://doi.org/10.1128/AEM.02624-10	journal	February 2011
Population genomics of Populus trichocarpa identifies signatures of selection and adaptive trait associations Evans, Luke M.; Slavov, Gancho T.; Rodgers-Melnick, Eli Nature Genetics, Vol. 46, Issue 10 https://doi.org/10.1038/ng.3075	journal	August 2014
moFF: a robust and automated approach to extract peptide ion intensities Argentini, Andrea; Goeminne, Ludger J. E.; Verheggen, Kenneth Nature Methods, Vol. 13, Issue 12 https://doi.org/10.1038/nmeth.4075	journal	November 2016
Complete Genome Sequences of Caldicellulosiruptor sp. Strain Rt8.B8, Caldicellulosiruptor sp. Strain Wai35.B1, and “ Thermoanaerobacter cellulolyticus ” Lee, Laura L.; Izquierdo, Javier A.; Blumer-Schuette, Sara E. Genome Announcements, Vol. 3, Issue 3 https://doi.org/10.1128/genomeA.00440-15	journal	May 2015
Comparison of three directly coupled HPLC MS/MS strategies for identification of proteins from complex mixtures: single-dimension LC-MS/MS, 2-phase MudPIT, and 3-phase MudPIT McDonald, W. Hayes; Ohi, Ryoma; Miyamoto, David T. International Journal of Mass Spectrometry, Vol. 219, Issue 1 https://doi.org/10.1016/S1387-3806(02)00563-8	journal	August 2002
Efficient Degradation of Lignocellulosic Plant Biomass, without Pretreatment, by the Thermophilic Anaerobe "Anaerocellum thermophilum" DSM 6725 Yang, Sung-Jae; Kataeva, Irina; Hamilton-Brehm, Scott D. Applied and Environmental Microbiology, Vol. 75, Issue 14, p. 4762-4769 https://doi.org/10.1128/AEM.00236-09	journal	May 2009
Use of Label-Free Quantitative Proteomics To Distinguish the Secreted Cellulolytic Systems of Caldicellulosiruptor bescii and Caldicellulosiruptor obsidiansis Lochner, Adriane; Giannone, Richard J.; Rodriguez, Miguel Applied and Environmental Microbiology, Vol. 77, Issue 12 https://doi.org/10.1128/AEM.02811-10	journal	April 2011
Phylogenetic, Microbiological, and Glycoside Hydrolase Diversities within the Extremely Thermophilic, Plant Biomass-Degrading Genus Caldicellulosiruptor Blumer-Schuette, S. E.; Lewis, D. L.; Kelly, R. M. Applied and Environmental Microbiology, Vol. 76, Issue 24, p. 8084-8092 https://doi.org/10.1128/AEM.01400-10	journal	October 2010
Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria Bergquist, Peter L.; Gibbs, Moreland D.; Morris, Daniel D. FEMS Microbiology Ecology, Vol. 28, Issue 2, p. 99-110 https://doi.org/10.1111/j.1574-6941.1999.tb00565.x	journal	February 1999
Identification of the C-Terminal GH5 Domain from CbCel9B/Man5A as the First Glycoside Hydrolase with Thermal Activation Property from a Multimodular Bifunctional Enzyme Wang, Rong; Gong, Li; Xue, Xianli PLOS ONE, Vol. 11, Issue 6 https://doi.org/10.1371/journal.pone.0156802	journal	June 2016
Faster SEQUEST Searching for Peptide Identification from Tandem Mass Spectra Diament, Benjamin J.; Noble, William Stafford Journal of Proteome Research, Vol. 10, Issue 9 https://doi.org/10.1021/pr101196n	journal	September 2011
Methylation by a Unique α-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725 Chung, Daehwan; Farkas, Joel; Huddleston, Jennifer R. PLoS ONE, Vol. 7, Issue 8 https://doi.org/10.1371/journal.pone.0043844	journal	August 2012
Genome Sequence of the Anaerobic, Thermophilic, and Cellulolytic Bacterium "Anaerocellum thermophilum" DSM 6725 Kataeva, I. A.; Yang, S. -J.; Dam, P. Journal of Bacteriology, Vol. 191, Issue 11 https://doi.org/10.1128/JB.00256-09	journal	April 2009
Semi-supervised learning for peptide identification from shotgun proteomics datasets Käll, Lukas; Canterbury, Jesse D.; Weston, Jason Nature Methods, Vol. 4, Issue 11 https://doi.org/10.1038/nmeth1113	journal	October 2007
Genome Stability in Engineered Strains of the Extremely Thermophilic Lignocellulose-Degrading Bacterium Caldicellulosiruptor bescii Williams-Rhaesa, Amanda M.; Poole, Farris L.; Dinsmore, Jessica T. Applied and Environmental Microbiology, Vol. 83, Issue 14 https://doi.org/10.1128/AEM.00444-17	journal	May 2017
Glycoside hydrolase inventory drives plant polysaccharide deconstruction by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus VanFossen, Amy L.; Ozdemir, Inci; Zelin, Samantha L. Biotechnology and Bioengineering, Vol. 108, Issue 7 https://doi.org/10.1002/bit.23093	journal	March 2011
Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization Zurawski, Jeffrey V.; Conway, Jonathan M.; Lee, Laura L. Applied and Environmental Microbiology, Vol. 81, Issue 20 https://doi.org/10.1128/AEM.01622-15	journal	August 2015
Deletion of Caldicellulosiruptor besciiCelA reveals its crucial role in the deconstruction of lignocellulosic biomass Young, Jenna; Chung, Daehwan; Bomble, Yannick J. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0142-6	journal	October 2014
The MPB83 Antigen from Mycobacterium bovis Contains O -Linked Mannose and (1 → 3)-Mannobiose Moieties Michell, Stephen L.; Whelan, Adam O.; Wheeler, Paul R. Journal of Biological Chemistry, Vol. 278, Issue 18 https://doi.org/10.1074/jbc.M207959200	journal	January 2003
An Intracellular Peptidyl-Prolyl cis / trans Isomerase Is Required for Folding and Activity of the Staphylococcus aureus Secreted Virulence Factor Nuclease Wiemels, Richard E.; Cech, Stephanie M.; Meyer, Nikki M. Journal of Bacteriology, Vol. 199, Issue 1 https://doi.org/10.1128/JB.00453-16	journal	October 2016
Large-scale analysis of the yeast proteome by multidimensional protein identification technology Washburn, Michael P.; Wolters, Dirk; Yates, John R. Nature Biotechnology, Vol. 19, Issue 3 https://doi.org/10.1038/85686	journal	March 2001
Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA Brunecky, R.; Alahuhta, M.; Xu, Q. Science, Vol. 342, Issue 6165, p. 1513-1516 https://doi.org/10.1126/science.1244273	journal	December 2013
Enzymatic Assembly of Overlapping DNA Fragments Gibson, Daniel G. Methods in Enzymology https://doi.org/10.1016/B978-0-12-385120-8.00015-2	book	May 2011
Rapid, accurate, computational discovery of Rho-independent transcription terminators illuminates their relationship to DNA uptake Kingsford, Carleton L.; Ayanbule, Kunmi; Salzberg, Steven L. Genome Biology, Vol. 8, Issue 2 https://doi.org/10.1186/gb-2007-8-2-r22	journal	January 2007
Construction of a Stable Replicating Shuttle Vector for Caldicellulosiruptor Species: Use for Extending Genetic Methodologies to Other Members of This Genus Chung, Daehwan; Cha, Minseok; Farkas, Joel PLoS ONE, Vol. 8, Issue 5, Article No. e62881 https://doi.org/10.1371/journal.pone.0062881	journal	May 2013
High-resolution genetic mapping of allelic variants associated with cell wall chemistry in Populus Muchero, Wellington; Guo, Jianjun; DiFazio, Stephen P. BMC Genomics, Vol. 16, Issue 1 https://doi.org/10.1186/s12864-015-1215-z	journal	January 2015
Molecular and Biochemical Analyses of CbCel9A/Cel48A, a Highly Secreted Multi-Modular Cellulase by Caldicellulosiruptor bescii during Growth on Crystalline Cellulose Yi, Zhuolin; Su, Xiaoyun; Revindran, Vanessa PLoS ONE, Vol. 8, Issue 12 https://doi.org/10.1371/journal.pone.0084172	journal	December 2013
Biochemical and Mutational Analyses of a Multidomain Cellulase/Mannanase from Caldicellulosiruptor bescii Su, Xiaoyun; Mackie, Roderick I.; Cann, Isaac K. O. Applied and Environmental Microbiology, Vol. 78, Issue 7 https://doi.org/10.1128/AEM.06814-11	journal	January 2012
Homologous Expression of the Caldicellulosiruptor bescii CelA Reveals that the Extracellular Protein Is Glycosylated Chung, Daehwan; Young, Jenna; Bomble, Yannick J. PLOS ONE, Vol. 10, Issue 3 https://doi.org/10.1371/journal.pone.0119508	journal	March 2015
Definition of the full extent of glycosylation of the 45-kilodalton glycoprotein of Mycobacterium tuberculosis. Dobos, K. M.; Khoo, K. H.; Swiderek, K. M. Journal of Bacteriology, Vol. 178, Issue 9 https://doi.org/10.1128/jb.178.9.2498-2506.1996	journal	May 1996
Effect of Prolyl Isomerase on the Folding Reactions of Staphylococcal Nuclease ^† Veeraraghavan, Sudha; Nall, Barry T.; Fink, Anthony L. Biochemistry, Vol. 36, Issue 49 https://doi.org/10.1021/bi971357r	journal	December 1997
Multidomain and Multifunctional Glycosyl Hydrolases from the Extreme Thermophile Caldicellulosiruptor Isolate Tok7B.1 Gibbs, Moreland D.; Reeves, Rosalind A.; Farrington, G. King Current Microbiology, Vol. 40, Issue 5, p. 333-340 https://doi.org/10.1007/s002849910066	journal	May 2000
Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains Zverlov, V.; Mahr, S.; Riedel, K. Microbiology, Vol. 144, Issue 2, p. 457-465 https://doi.org/10.1099/00221287-144-2-457	journal	February 1998
The genome portal of the Department of Energy Joint Genome Institute: 2014 updates Nordberg, Henrik; Cantor, Michael; Dusheyko, Serge Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1069	journal	November 2013
Overcoming restriction as a barrier to DNA transformation in Caldicellulosiruptor species results in efficient marker replacement Chung, Daehwan; Farkas, Joel; Westpheling, Janet Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-82	journal	January 2013
Glycoside Hydrolase Gene Cluster of Acidothermus cellulolyticus Ding, Shi-You; Adney, William S.; Vinzant, Todd B. Applications of Enzymes to Lignocellulosics https://doi.org/10.1021/bk-2003-0855.ch020	book	August 2003
Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii Farkas, Joel; Chung, Daehwan; Cha, Minseok Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 1, p. 41-49 https://doi.org/10.1007/s10295-012-1202-1	journal	November 2012
Molecular and Biochemical Analyses of the GH44 Module of CbMan5B/Cel44A, a Bifunctional Enzyme from the Hyperthermophilic Bacterium Caldicellulosiruptor bescii Ye, Libin; Su, Xiaoyun; Schmitz, George E. Applied and Environmental Microbiology, Vol. 78, Issue 19 https://doi.org/10.1128/AEM.02009-12	journal	July 2012
Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass Cha, Minseok; Chung, Daehwan; Elkins, James G. Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-85	journal	January 2013
Detecting protein and post-translational modifications in single cells with iDentification and qUantification sEparaTion (DUET) Zhang, Yandong; Sohn, Changho; Lee, Seoyeon Communications Biology, Vol. 3, Issue 1 https://doi.org/10.1038/s42003-020-01132-8	journal	August 2020

Cited By (7)

Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species Conway, Jonathan M.; Crosby, James R.; Hren, Andrew P. AIChE Journal, Vol. 64, Issue 12 https://doi.org/10.1002/aic.16354	journal	August 2018
Parsing in vivo and in vitro contributions to microcrystalline cellulose hydrolysis by multidomain glycoside hydrolases in the Caldicellulosiruptor bescii secretome Conway, Jonathan M.; Crosby, James R.; McKinley, Bennett S. Biotechnology and Bioengineering, Vol. 115, Issue 10 https://doi.org/10.1002/bit.26773	journal	July 2018
Thermoanaerobacter species differ in their potential to reduce organic acids to their corresponding alcohols Hitschler, Lisa; Kuntz, Michelle; Langschied, Felix Applied Microbiology and Biotechnology, Vol. 102, Issue 19 https://doi.org/10.1007/s00253-018-9210-3	journal	July 2018
Genomic and physiological analyses reveal that extremely thermophilic Caldicellulosiruptor changbaiensis deploys uncommon cellulose attachment mechanisms Khan, Asma M. A. M.; Mendoza, Carl; Hauk, Valerie J. Journal of Industrial Microbiology & Biotechnology, Vol. 46, Issue 9-10 https://doi.org/10.1007/s10295-019-02222-1	journal	August 2019
The diversity and specificity of the extracellular proteome in the cellulolytic bacterium Caldicellulosiruptor bescii is driven by the nature of the cellulosic growth substrate Poudel, Suresh; Giannone, Richard J.; Basen, Mirko Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1076-1	journal	March 2018
Deletion of a single glycosyltransferase in Caldicellulosiruptor bescii eliminates protein glycosylation and growth on crystalline cellulose Russell, Jordan; Kim, Sun-Ki; Duma, Justin Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1266-x	journal	September 2018
The GH10 and GH48 dual-functional catalytic domains from a multimodular glycoside hydrolase synergize in hydrolyzing both cellulose and xylan Chu, Yindi; Hao, Zhenzhen; Wang, Kaikai Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1617-2	journal	December 2019

Similar Records

Genus-wide assessment of lignocellulose utilization in the extremely thermophilic Caldicellulosiruptor by genomic, pan-genomic and metagenomic analysis

Journal Article · 2018 · Applied and Environmental Microbiology · OSTI ID:1432145

Lee, Laura L.; Blumer-Schuette, Sara E.; Izquierdo, Javier A.; +12 more

Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization

Journal Article · 2015 · Applied and Environmental Microbiology · OSTI ID:1335326

Zurawski, Jeffrey V.; Conway, Jonathan M.; Lee, Laura; +6 more

Deletion of Caldicellulosiruptor bescii CelA reveals its crucial role in the deconstruction of lignocellulosic biomass

Program Document · 2014 · Biotechnology for Biofuels · OSTI ID:1163406

Young, Jenna; Chung, Daehwan; Bomble, Yannick J.; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES

Title: Functional Analysis of the Glucan Degradation Locus in Caldicellulosiruptor bescii Reveals Essential Roles of Component Glycoside Hydrolases in Plant Biomass Deconstruction

Citation Formats

References (60)

Cited By (7)

Similar Records

Related Subjects