Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae

Chen, Gao; Shouakar-Stash, Orfan; Phillips, Elizabeth; Justicia-Leon, Shandra D.; Gilevska, Tetyana; Sherwood Lollar, Barbara; Mack, E. Erin; Seger, Edward S.; Löffler, Frank E.

doi:10.1021/acs.est.8b01583

Title: Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae

Journal Article · 05 July 2018 · Environmental Science and Technology

DOI: https://doi.org/10.1021/acs.est.8b01583 · OSTI ID:1474553

Chen, Gao ^[1]; Shouakar-Stash, Orfan ^[2]; Phillips, Elizabeth ^[3]; Justicia-Leon, Shandra D. ^[4]; Gilevska, Tetyana ^[3]; Sherwood Lollar, Barbara ^[3]; Mack, E. Erin ^[5]; Seger, Edward S. ^[6];

^[7]

Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, and Dept. of Microbiology
Isotope Tracer Technologies Inc. (IT2), Waterloo, Ontario (Canada); Univ. of Waterloo, Waterloo, Ontario (Canada). Dept. of Earth and Environmental Sciences; Univ. of Guelph, Guelph, Ontario (Canada). School of Engineering,
Univ. of Toronto, Toronto, Ontario (Canada). Dept. of Earth Sciences
Georgia Inst. of Technology, Atlanta, GA (United States). School of Biology
DuPont de Nemours and Company, Wilmington, DE (United States). DuPont Corporate Remediation Group
The Chemours Company, Wilmington, DE (United States)
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, and Dept. of Microbiology; Univ. of TN and Oak Ridge National Lab., Oak Ridge, TN (United States). Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division; University of Tennessee, Knoxville, TN (United States). Dept. of Biosystems Engineering and Soil Science

Dichloromethane (DCM) is a probable human carcinogen and frequent groundwater contaminant and contributes to stratospheric ozone layer depletion. DCM is degraded by aerobes harboring glutathione-dependent DCM dehalogenases; however, DCM contamination occurs in oxygen-deprived environments, and much less is known about anaerobic DCM metabolism. Some members of the Peptococcaceae family convert DCM to environmentally benign products including acetate, formate, hydrogen (H₂), and inorganic chloride under strictly anoxic conditions. The current study applied stable carbon and chlorine isotope fractionation measurements to the axenic culture Dehalobacterium formicoaceticum and to the consortium RM comprising DCM degrader Candidatus Dichloromethanomonas elyunquensis. Degradation-associated carbon and chlorine isotope enrichment factors (ε_C and ε_Cl) of -42.4 ± 0.7‰ and -5.3 ± 0.1‰, respectively, were measured in D. formicoaceticum cultures. A similar ε_Cl of -5.2 ± 0.1‰, but a substantially lower ε_C of -18.3 ± 0.2‰, were determined for Ca. Dichloromethanomonas elyunquensis. The ε_C and εCl values resulted in distinctly different dual element C–Cl isotope correlations (Λ^C/Cl = Δδ¹³C/Δδ³⁷Cl) of 7.89 ± 0.12 and 3.40 ± 0.03 for D. formicoaceticum and Ca. Dichloromethanomonas elyunquensis, respectively. The distinct Λ^C/Cl values obtained for the two cultures imply mechanistically distinct C–Cl bond cleavage reactions, suggesting that members of Peptococcaceae employ different pathways to metabolize DCM. These findings emphasize the utility of dual carbon–chlorine isotope analysis to pinpoint DCM degradation mechanisms and to provide an additional line of evidence that detoxification is occurring at DCM-contaminated sites.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1474553

Journal Information:: Environmental Science and Technology, Vol. 52, Issue 15; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 22 works

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References (65)

Stable Carbon Isotope Enrichment Factors for cis -1,2-Dichloroethene and Vinyl Chloride Reductive Dechlorination by Dehalococcoides Fletcher, Kelly E.; Nijenhuis, Ivonne; Richnow, Hans-Hermann Environmental Science & Technology, Vol. 45, Issue 7 https://doi.org/10.1021/es103728q	journal	April 2011
A review of bacterial methyl halide degradation: biochemistry, genetics and molecular ecology McDonald, I. R.; Warner, K. L.; McAnulla, C. Environmental Microbiology, Vol. 4, Issue 4 https://doi.org/10.1046/j.1462-2920.2002.00290.x	journal	April 2002
Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results: Guidelines and recommended terms for expressing stable isotope results Coplen, Tyler B. Rapid Communications in Mass Spectrometry, Vol. 25, Issue 17 https://doi.org/10.1002/rcm.5129	journal	August 2011
Substrate Hydrophobicity and Cell Composition Influence the Extent of Rate Limitation and Masking of Isotope Fractionation during Microbial Reductive Dehalogenation of Chlorinated Ethenes Renpenning, Julian; Rapp, Insa; Nijenhuis, Ivonne Environmental Science & Technology, Vol. 49, Issue 7 https://doi.org/10.1021/es506108j	journal	March 2015
Bacterial growth with chlorinated methanes. Leisinger, T.; Braus-Stromeyer, S. A. Environmental Health Perspectives, Vol. 103, Issue suppl 5 https://doi.org/10.1289/ehp.95103s433	journal	June 1995
Extending Limits of Chlorine Kinetic Isotope Effects Świderek, Katarzyna; Paneth, Piotr The Journal of Organic Chemistry, Vol. 77, Issue 11 https://doi.org/10.1021/jo300682f	journal	May 2012
Influence of Mass-Transfer Limitations on Carbon Isotope Fractionation during Microbial Dechlorination of Trichloroethene Aeppli, Christoph; Berg, Michael; Cirpka, Olaf A. Environmental Science & Technology, Vol. 43, Issue 23 https://doi.org/10.1021/es901481b	journal	October 2009
Influence of mass transfer on stable isotope fractionation Thullner, Martin; Fischer, Anko; Richnow, Hans-Hermann Applied Microbiology and Biotechnology, Vol. 97, Issue 2 https://doi.org/10.1007/s00253-012-4537-7	journal	November 2012
Large Carbon Isotope Fractionation during Biodegradation of Chloroform by Dehalobacter Cultures Chan, Calvin C. H.; Mundle, Scott O. C.; Eckert, Thomas Environmental Science & Technology https://doi.org/10.1021/es3010317	journal	August 2012
Carbon and Chlorine Isotope Fractionation Patterns Associated with Different Engineered Chloroform Transformation Reactions Torrentó, Clara; Palau, Jordi; Rodríguez-Fernández, Diana Environmental Science & Technology, Vol. 51, Issue 11 https://doi.org/10.1021/acs.est.7b00679	journal	May 2017
Hydrogen Isotope Fractionation As a Tool to Identify Aerobic and Anaerobic PAH Biodegradation Kümmel, Steffen; Starke, Robert; Chen, Gao Environmental Science & Technology, Vol. 50, Issue 6 https://doi.org/10.1021/acs.est.5b04819	journal	February 2016
Reductive Outer-Sphere Single Electron Transfer Is an Exception Rather than the Rule in Natural and Engineered Chlorinated Ethene Dehalogenation Heckel, Benjamin; Cretnik, Stefan; Kliegman, Sarah Environmental Science & Technology, Vol. 51, Issue 17 https://doi.org/10.1021/acs.est.7b01447	journal	August 2017
Measurement of Henry's law constants for C1 and C2 chlorinated hydrocarbons Gossett, James M. Environmental Science & Technology, Vol. 21, Issue 2 https://doi.org/10.1021/es00156a012	journal	February 1987
Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria Miller, L. G.; Kalin, R. M.; McCauley, S. E. Proceedings of the National Academy of Sciences, Vol. 98, Issue 10 https://doi.org/10.1073/pnas.101129798	journal	May 2001
Compound-specific chlorine isotope ratios of TCE, PCE and DCE isomers by direct injection using CF-IRMS Shouakar-Stash, Orfan; Drimmie, Robert J.; Zhang, Min Applied Geochemistry, Vol. 21, Issue 5 https://doi.org/10.1016/j.apgeochem.2006.02.006	journal	May 2006
Stable isotope fractionation to investigate natural transformation mechanisms of organic contaminants: principles, prospects and limitations Elsner, Martin Journal of Environmental Monitoring, Vol. 12, Issue 11 https://doi.org/10.1039/c0em00277a	journal	January 2010
Isolation and characterization of Dehalobacterium formicoaceticum gen. nov. sp. nov., a strictly anaerobic bacterium utilizing dichloromethane as source of carbon and energy Mägli, Andreas; Wendt, Matthias; Leisinger, T. Archives of Microbiology, Vol. 166, Issue 2 https://doi.org/10.1007/s002030050362	journal	August 1996
Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria Nadalig, Thierry; Greule, Markus; Bringel, Françoise MicrobiologyOpen, Vol. 2, Issue 6 https://doi.org/10.1002/mbo3.124	journal	September 2013
‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family Kleindienst, Sara; Higgins, Steven A.; Tsementzi, Despina Systematic and Applied Microbiology, Vol. 40, Issue 3 https://doi.org/10.1016/j.syapm.2016.12.001	journal	April 2017
New Evaluation Scheme for Two-Dimensional Isotope Analysis to Decipher Biodegradation Processes: Application to Groundwater Contamination by MTBE Zwank, Luc; Berg, Michael; Elsner, Martin Environmental Science & Technology, Vol. 39, Issue 4 https://doi.org/10.1021/es049650j	journal	February 2005
Carbon and nitrogen isotope analysis of atrazine and desethylatrazine at sub-microgram per liter concentrations in groundwater Schreglmann, Kathrin; Hoeche, Martina; Steinbeiss, Sibylle Analytical and Bioanalytical Chemistry, Vol. 405, Issue 9 https://doi.org/10.1007/s00216-012-6616-0	journal	December 2012
Relative Contributions of Dehalobacter and Zerovalent Iron in the Degradation of Chlorinated Methanes Lee, Matthew; Wells, Eliza; Wong, Yie Kuan Environmental Science & Technology, Vol. 49, Issue 7 https://doi.org/10.1021/es5052364	journal	March 2015
Mutualistic interaction between dichloromethane- and chloromethane-degrading bacteria in an anaerobic mixed culture: Mutualistic degradation of chlorinated methanes Chen, Gao; Kleindienst, Sara; Griffiths, Daniel R. Environmental Microbiology, Vol. 19, Issue 11 https://doi.org/10.1111/1462-2920.13945	journal	October 2017
A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane Vannelli, T.; Messmer, M.; Studer, A. Proceedings of the National Academy of Sciences, Vol. 96, Issue 8 https://doi.org/10.1073/pnas.96.8.4615	journal	April 1999
Headspace Analysis: A New Application for Isotopic Characterization of Dissolved Organic Contaminants Slater, Gregory F.; Dempster, Helen S.; Sherwood Lollar, Barbara Environmental Science & Technology, Vol. 33, Issue 1 https://doi.org/10.1021/es9803254	journal	January 1999
Dichloromethane-degrading bacteria in the genomic age Muller, Emilie E. L.; Bringel, Françoise; Vuilleumier, Stéphane Research in Microbiology, Vol. 162, Issue 9 https://doi.org/10.1016/j.resmic.2011.01.008	journal	November 2011
Evaluating Pesticide Degradation in the Environment: Blind Spots and Emerging Opportunities Fenner, Kathrin; Canonica, Silvio; Wackett, Lawrence P. Science, Vol. 341, Issue 6147 https://doi.org/10.1126/science.1236281	journal	August 2013
The increasing threat to stratospheric ozone from dichloromethane Hossaini, Ryan; Chipperfield, Martyn P.; Montzka, Stephen A. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15962	journal	June 2017
Determination of Compound-Specific Carbon Isotope Ratios of Chlorinated Methanes, Ethanes, and Ethenes in Aqueous Samples Hunkeler, Daniel; Aravena, Ramon Environmental Science & Technology, Vol. 34, Issue 13 https://doi.org/10.1021/es991178s	journal	July 2000
The Expression of Isotope Effects on Enzyme-Catalyzed Reactions Northrop, D. B. Annual Review of Biochemistry, Vol. 50, Issue 1 https://doi.org/10.1146/annurev.bi.50.070181.000535	journal	June 1981
A New Concept Linking Observable Stable Isotope Fractionation to Transformation Pathways of Organic Pollutants Elsner, Martin; Zwank, Luc; Hunkeler, Daniel Environmental Science & Technology, Vol. 39, Issue 18 https://doi.org/10.1021/es0504587	journal	September 2005
Stable Carbon Isotope Evidence for Intrinsic Bioremediation of Tetrachloroethene and Trichloroethene at Area 6, Dover Air Force Base Sherwood Lollar, B.; Slater, G. F.; Sleep, B. Environmental Science & Technology, Vol. 35, Issue 2 https://doi.org/10.1021/es001227x	journal	January 2001
Stable Carbon Isotope Fractionation of 1,2-Dichloropropane during Dichloroelimination by Dehalococcoides Populations Fletcher, Kelly E.; Löffler, Frank E.; Richnow, Hans-Hermann Environmental Science & Technology, Vol. 43, Issue 18 https://doi.org/10.1021/es900365x	journal	August 2009
Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane Heraty, L. J.; Fuller, M. E.; Huang, L. Organic Geochemistry, Vol. 30, Issue 8 https://doi.org/10.1016/S0146-6380(99)00062-5	journal	August 1999
Rayleigh equation for evolution of stable isotope ratios in contaminant decay chains Höhener, Patrick; Atteia, Olivier Geochimica et Cosmochimica Acta, Vol. 126 https://doi.org/10.1016/j.gca.2013.10.036	journal	February 2014
Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications. Fetzner, S.; Lingens, F. Microbiological Reviews, Vol. 58, Issue 4 https://doi.org/10.1128/MMBR.58.4.641-685.1994	journal	January 1994
Carbon and hydrogen stable isotope fractionation associated with the anaerobic degradation of propane and butane by marine sulfate-reducing bacteria: Degradation of gaseous alkanes - isotope fractionation Jaekel, Ulrike; Vogt, Carsten; Fischer, Anko Environmental Microbiology, Vol. 16, Issue 1 https://doi.org/10.1111/1462-2920.12251	journal	September 2013
New guidelines for reporting stable hydrogen, carbon, and oxygen isotope-ratio data Coplen, Tyler B. Geochimica et Cosmochimica Acta, Vol. 60, Issue 17 https://doi.org/10.1016/0016-7037(96)00263-3	journal	September 1996
A New Interpretation of Chlorine Leaving Group Kinetic Isotope Effects; A Theoretical Approach Dybała-Defratyka, Agnieszka; Rostkowski, Michał; Matsson, Olle The Journal of Organic Chemistry, Vol. 69, Issue 15 https://doi.org/10.1021/jo049327z	journal	July 2004
Complete chloroform dechlorination by organochlorine respiration and fermentation: Microbial chloroform metabolism Lee, Matthew; Low, Adrian; Zemb, Olivier Environmental Microbiology, Vol. 14, Issue 4 https://doi.org/10.1111/j.1462-2920.2011.02656.x	journal	November 2011
Isotopic Evidence Suggests Different Initial Reaction Mechanisms for Anaerobic Benzene Biodegradation Mancini, Silvia A.; Devine, Cheryl E.; Elsner, Martin Environmental Science & Technology, Vol. 42, Issue 22 https://doi.org/10.1021/es801107g	journal	November 2008
Pressure-monitored headspace analysis combined with compound-specific isotope analysis to measure isotope fractionation in gas-producing reactions: Pressure-monitored headspace analysis and CSIA to measure ε values Mundle, Scott O. C.; Vandersteen, Adelle A.; Lacrampe-Couloume, Georges Rapid Communications in Mass Spectrometry, Vol. 27, Issue 15 https://doi.org/10.1002/rcm.6625	journal	June 2013
Dichloromethane biodegradation in multi-contaminated groundwater: Insights from biomolecular and compound-specific isotope analyses Hermon, L.; Denonfoux, J.; Hellal, J. Water Research, Vol. 142 https://doi.org/10.1016/j.watres.2018.05.057	journal	October 2018
Impact of Bioavailability Restrictions on Microbially Induced Stable Isotope Fractionation. 2. Experimental Evidence Kampara, Makeba; Thullner, Martin; Richnow, Hans H. Environmental Science & Technology, Vol. 42, Issue 17 https://doi.org/10.1021/es702781x	journal	July 2008
C and Cl Isotope Fractionation of 1,2-Dichloroethane Displays Unique δ ¹³ C/δ ³⁷ Cl Patterns for Pathway Identification and Reveals Surprising C–Cl Bond Involvement in Microbial Oxidation Palau, Jordi; Cretnik, Stefan; Shouakar-Stash, Orfan Environmental Science & Technology, Vol. 48, Issue 16 https://doi.org/10.1021/es5031917	journal	July 2014
Factors controlling the carbon isotope fractionation of tetra- and trichloroethene during reductive dechlorination by Sulfurospirillum ssp. and Desulfitobacterium sp. strain PCE-S: Carbon isotope fractionation during reductive dechlorination Cichocka, Danuta; Siegert, Michael; Imfeld, GwenaÃ«l FEMS Microbiology Ecology, Vol. 62, Issue 1 https://doi.org/10.1111/j.1574-6941.2007.00367.x	journal	October 2007
Molecular and carbon isotopic characterization of an anaerobic stable enrichment culture containing Dehalobacterium sp. during dichloromethane fermentation Trueba-Santiso, Alba; Parladé, Eloi; Rosell, Mònica Science of The Total Environment, Vol. 581-582 https://doi.org/10.1016/j.scitotenv.2016.12.174	journal	March 2017
Diffusion Sampler for Compound Specific Carbon Isotope Analysis of Dissolved Hydrocarbon Contaminants Passeport, Elodie; Landis, Richard; Mundle, Scott O. C. Environmental Science & Technology, Vol. 48, Issue 16 https://doi.org/10.1021/es501632g	journal	August 2014
Impact of Bioavailability Restrictions on Microbially Induced Stable Isotope Fractionation. 1. Theoretical Calculation Thullner, Martin; Kampara, Makeba; Richnow, Hans H. Environmental Science & Technology, Vol. 42, Issue 17 https://doi.org/10.1021/es702782c	journal	July 2008
Chlorinated Solvents in Groundwater of the United States Moran, Michael J.; Zogorski, John S.; Squillace, Paul J. Environmental Science & Technology, Vol. 41, Issue 1 https://doi.org/10.1021/es061553y	journal	January 2007
Chloroform respiration to dichloromethane by a Dehalobacter population: Growth-linked reductive dechlorination of chloroform Grostern, Ariel; Duhamel, Melanie; Dworatzek, Sandra Environmental Microbiology, Vol. 12, Issue 4 https://doi.org/10.1111/j.1462-2920.2009.02150.x	journal	January 2010
Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium Chen, Gao; Murdoch, Robert W.; Mack, E. Erin Genome Announcements, Vol. 5, Issue 37 https://doi.org/10.1128/genomeA.00897-17	journal	September 2017
Evaluation of Isotopic Enrichment Factors for the Biodegradation of Chlorinated Ethenes Using a Parameter Estimation Model: Toward an Improved Quantification of Biodegradation Morrill, Penny L.; Sleep, Brent E.; Slater, Gregory F. Environmental Science & Technology, Vol. 40, Issue 12 https://doi.org/10.1021/es051513e	journal	May 2006
Natural chlorine isotope variations Kaufmann, Ronald; Long, Austin; Bentley, Harold Nature, Vol. 309, Issue 5966 https://doi.org/10.1038/309338a0	journal	May 1984
The elusive roles of bacterial glutathione S- transferases: new lessons from genomes Pagni, S. Vuilleumier, M. Applied Microbiology and Biotechnology, Vol. 58, Issue 2 https://doi.org/10.1007/s00253-001-0836-0	journal	January 2002
Monitoring Microbial Dechlorination of Tetrachloroethene (PCE) in Groundwater Using Compound-Specific Stable Carbon Isotope Ratios: Microcosm and Field Studies Hunkeler, D.; Aravena, R.; Butler, B. J. Environmental Science & Technology, Vol. 33, Issue 16 https://doi.org/10.1021/es981282u	journal	August 1999
Insights into Enzyme Kinetics of Chloroethane Biodegradation Using Compound Specific Stable Isotopes Lollar, Barbara Sherwood; Hirschorn, Sarah; Mundle, Scott O. C. Environmental Science & Technology, Vol. 44, Issue 19 https://doi.org/10.1021/es101330r	journal	October 2010
A D esulfitobacterium sp. strain PR reductively dechlorinates both 1,1,1-trichloroethane and chloroform : Strain PR dechlorinates TCA and chloroform Ding, Chang; Zhao, Siyan; He, Jianzhong Environmental Microbiology, Vol. 16, Issue 11 https://doi.org/10.1111/1462-2920.12387	journal	February 2014
Stable carbon isotope fractionation during degradation of dichloromethane by methylotrophic bacteria Nikolausz, Marcell; Nijenhuis, Ivonne; Ziller, Katja Environmental Microbiology, Vol. 8, Issue 1 https://doi.org/10.1111/j.1462-2920.2005.00878.x	journal	January 2006
Impact of cell density on microbially induced stable isotope fractionation Kampara, Makeba; Thullner, Martin; Harms, Hauke Applied Microbiology and Biotechnology, Vol. 81, Issue 5 https://doi.org/10.1007/s00253-008-1755-0	journal	January 2009
Analysis of Compound-Specific Chlorine Stable Isotopes of Vinyl Chloride by Continuous Flow–Isotope Ratio Mass Spectrometry (FC–IRMS) Shouakar-Stash, Orfan; Frape, S. K.; Aravena, R. Environmental Forensics, Vol. 10, Issue 4 https://doi.org/10.1080/15275920903347628	journal	December 2009
Metabolism of Dichloromethane by the Strict Anaerobe Dehalobacterium formicoaceticum Mägli, Andreas; Messmer, Michael; Leisinger, Thomas Applied and Environmental Microbiology, Vol. 64, Issue 2 https://doi.org/10.1128/AEM.64.2.646-650.1998	journal	January 1998
Volatile organic compounds in the nation's ground water and drinking-water supply wells Zogorski, John S.; Carter, Janet M.; Ivahnenko, Tamara USGS https://doi.org/10.3133/cir1292	report	January 2006
Dichloromethane dehalogenase of Hyphomicrobium sp. strain DM2. Kohler-Staub, D.; Leisinger, T. Journal of Bacteriology, Vol. 162, Issue 2 https://doi.org/10.1128/jb.162.2.676-681.1985	journal	January 1985
Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications Fetzner, S.; Lingens, F. Microbiological Reviews, Vol. 58, Issue 4 https://doi.org/10.1128/mr.58.4.641-685.1994	journal	December 1994

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