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Title: Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum

Abstract

This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities. Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared. The EPSs from both strains mainly contained polysaccharide (41–64%, w/w, dry basis) and protein (25–40%, w/w, dry basis), which was shown by the morphology study with an AFM. The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose. Glucose and arabinose took up to 82–90% (w/w, dry basis) of the total polysaccharide. The structure and functional groups of EPSs were determined by FTIR and NMR. The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose. The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549. The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1]; ORCiD logo [3];  [1];  [1]; ORCiD logo [1]
  1. Clemson Univ., SC (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1474532
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Carbohydrate Polymers
Additional Journal Information:
Journal Volume: 195; Journal Issue: C; Journal ID: ISSN 0144-8617
Publisher:
Applied Science Direct
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Xiao, Rui, Yang, Xi, Li, Mi, Li, Xiang, Wei, Yanzhang, Cao, Min, Ragauskas, Arthur, Thies, Mark, Ding, Junhuan, and Zheng, Yi. Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum. United States: N. p., 2018. Web. https://doi.org/10.1016/j.carbpol.2018.04.126.
Xiao, Rui, Yang, Xi, Li, Mi, Li, Xiang, Wei, Yanzhang, Cao, Min, Ragauskas, Arthur, Thies, Mark, Ding, Junhuan, & Zheng, Yi. Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum. United States. https://doi.org/10.1016/j.carbpol.2018.04.126
Xiao, Rui, Yang, Xi, Li, Mi, Li, Xiang, Wei, Yanzhang, Cao, Min, Ragauskas, Arthur, Thies, Mark, Ding, Junhuan, and Zheng, Yi. Tue . "Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum". United States. https://doi.org/10.1016/j.carbpol.2018.04.126. https://www.osti.gov/servlets/purl/1474532.
@article{osti_1474532,
title = {Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum},
author = {Xiao, Rui and Yang, Xi and Li, Mi and Li, Xiang and Wei, Yanzhang and Cao, Min and Ragauskas, Arthur and Thies, Mark and Ding, Junhuan and Zheng, Yi},
abstractNote = {This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities. Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared. The EPSs from both strains mainly contained polysaccharide (41–64%, w/w, dry basis) and protein (25–40%, w/w, dry basis), which was shown by the morphology study with an AFM. The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose. Glucose and arabinose took up to 82–90% (w/w, dry basis) of the total polysaccharide. The structure and functional groups of EPSs were determined by FTIR and NMR. The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose. The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549. The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.},
doi = {10.1016/j.carbpol.2018.04.126},
journal = {Carbohydrate Polymers},
number = C,
volume = 195,
place = {United States},
year = {2018},
month = {5}
}

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