Clay Flocculation Effect on Microbial Community Composition in Water and Sediment
Abstract
Clay-based flocculation techniques have been developed to mitigate harmful algal blooms; however, the potential ecological impacts on the microbial community are poorly understood. In this study, chemical measurements were combined with 16S rRNA sequencing to characterize the microbial community response to different flocculation techniques, including controls, clay flocculation, clay flocculation with zeolite, and clay flocculation with O2 added zeolite capping. Sediment bacterial biomass measured by PLFA were not significantly altered by the various flocculation techniques used. However, 16S rRNA sequencing revealed differences in water microbial community structure between treatments with and without zeolite capping. The differences were related to significant reductions of total nitrogen (TN), total phosphorus (TP) and ammonia (NH4+) concentration and increase of nitrate (NO3-) concentration in zeolite and O2 loaded zeolite capping. The relative abundance of ammonia oxidizing bacteria increased four-fold in zeolite capping microcosms, suggesting zeolite promoted absorbed ammonia removal in the benthic zone. Zeolite-capping promoted bacteria nitrogen cycling activities at the water-sediment interface. Potential pathogens that are usually adapted to eutrophic water bodies were reduced after clay flocculation. This study demonstrated clay flocculation did not decrease bacterial populations overall and may reduce regulatory indicators and pathogenic contaminants in water. Zeolite capping may also help preventmore »
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States)
- Chinese Academy of Sciences (CAS), Beijing (China)
- Univ. of Tennessee, Knoxville, TN (United States); Michigan Technological Univ., Houghton, MI (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1474524
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Environmental Science
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: n/a; Journal ID: ISSN 2296-665X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES
Citation Formats
Chen, Chunyi, Pan, Gang, Shi, Wenqing, Xu, Feng, Techtmann, Stephen M., Pfiffner, Susan M., and Hazen, Terry C. Clay Flocculation Effect on Microbial Community Composition in Water and Sediment. United States: N. p., 2018.
Web. doi:10.3389/fenvs.2018.00060.
Chen, Chunyi, Pan, Gang, Shi, Wenqing, Xu, Feng, Techtmann, Stephen M., Pfiffner, Susan M., & Hazen, Terry C. Clay Flocculation Effect on Microbial Community Composition in Water and Sediment. United States. doi:10.3389/fenvs.2018.00060.
Chen, Chunyi, Pan, Gang, Shi, Wenqing, Xu, Feng, Techtmann, Stephen M., Pfiffner, Susan M., and Hazen, Terry C. Mon .
"Clay Flocculation Effect on Microbial Community Composition in Water and Sediment". United States. doi:10.3389/fenvs.2018.00060. https://www.osti.gov/servlets/purl/1474524.
@article{osti_1474524,
title = {Clay Flocculation Effect on Microbial Community Composition in Water and Sediment},
author = {Chen, Chunyi and Pan, Gang and Shi, Wenqing and Xu, Feng and Techtmann, Stephen M. and Pfiffner, Susan M. and Hazen, Terry C.},
abstractNote = {Clay-based flocculation techniques have been developed to mitigate harmful algal blooms; however, the potential ecological impacts on the microbial community are poorly understood. In this study, chemical measurements were combined with 16S rRNA sequencing to characterize the microbial community response to different flocculation techniques, including controls, clay flocculation, clay flocculation with zeolite, and clay flocculation with O2 added zeolite capping. Sediment bacterial biomass measured by PLFA were not significantly altered by the various flocculation techniques used. However, 16S rRNA sequencing revealed differences in water microbial community structure between treatments with and without zeolite capping. The differences were related to significant reductions of total nitrogen (TN), total phosphorus (TP) and ammonia (NH4+) concentration and increase of nitrate (NO3-) concentration in zeolite and O2 loaded zeolite capping. The relative abundance of ammonia oxidizing bacteria increased four-fold in zeolite capping microcosms, suggesting zeolite promoted absorbed ammonia removal in the benthic zone. Zeolite-capping promoted bacteria nitrogen cycling activities at the water-sediment interface. Potential pathogens that are usually adapted to eutrophic water bodies were reduced after clay flocculation. This study demonstrated clay flocculation did not decrease bacterial populations overall and may reduce regulatory indicators and pathogenic contaminants in water. Zeolite capping may also help prevent nutrients from being released back into the water thus preventing additional algal blooms.},
doi = {10.3389/fenvs.2018.00060},
journal = {Frontiers in Environmental Science},
number = n/a,
volume = 6,
place = {United States},
year = {2018},
month = {6}
}
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Works referenced in this record:
Ammonium Transformation in a Nitrogen-Rich Tidal Freshwater Marsh
journal, June 2006
- Gribsholt, Britta; Struyf, Eric; Tramper, Anton
- Biogeochemistry, Vol. 80, Issue 3
Flocculation characteristics of organo-modified clay particles in poly(L-lactide) / montmorillonite hybrid systems
journal, January 2004
- Nam, Pham Hoai; Fujimori, Atsuhiro; Masuko, Toru
- e-Polymers, Vol. 4, Issue 1
Anoxic N2 fluxes from freshwater sediments in the absence of oxidized nitrogen compounds
journal, February 1998
- Van Luijn, Francien; Boers, Paul C. M.; Lijklema, Lambertus
- Water Research, Vol. 32, Issue 2
Nitrobacter and Nitrospira genera as representatives of nitrite-oxidizing bacteria: Detection, quantification and growth along the lower Seine River (France)
journal, December 2005
- Cébron, Aurélie; Garnier, Josette
- Water Research, Vol. 39, Issue 20
Determination of phospholipid fatty acid structures and stable carbon isotope compositions of deep-sea sediments of the Northwest Pacific, ODP site 1179
journal, February 2006
- Mills, Christopher T.; Dias, Robert F.; Graham, Dennis
- Marine Chemistry, Vol. 98, Issue 2-4
Controlling Harmful Algal Blooms Through Clay Flocculation1
journal, March 2004
- Sengco, Mario R.; Anderson, Donald M.
- The Journal of Eukaryotic Microbiology, Vol. 51, Issue 2
Phosphorus partitioning in a shallow lake: implications for water quality management
journal, March 2007
- Spears, B. M.; Carvalho, L.; Paterson, D. M.
- Water and Environment Journal, Vol. 21, Issue 1
Application of a three-dimensional eutrophication model for the Beijing Guanting Reservoir, China
journal, April 2011
- He, Guojian; Fang, Hongwei; Bai, Sen
- Ecological Modelling, Vol. 222, Issue 8
Assessment of the number, biomass, and cell size of bacteria in different soils using the “cascade” filtration method
journal, March 2015
- Polyanskaya, L. M.; Pinchuk, I. P.; Zvyagintsev, D. G.
- Eurasian Soil Science, Vol. 48, Issue 3
Effects of a modified zeolite on P and N processes and fluxes across the lake sediment–water interface using core incubations
journal, January 2010
- Gibbs, Max; Özkundakci, Deniz
- Hydrobiologia, Vol. 661, Issue 1
Active Autotrophic Ammonia-Oxidizing Bacteria in Biofilm Enrichments from Simulated Creek Ecosystems at Two Ammonium Concentrations Respond to Temperature Manipulation
journal, September 2011
- Avrahami, Sharon; Jia, Zhongjun; Neufeld, Josh D.
- Applied and Environmental Microbiology, Vol. 77, Issue 20
A broken biogeochemical cycle
journal, October 2011
- Elser, James; Bennett, Elena
- Nature, Vol. 478, Issue 7367
Assessing Nitrification and Denitrification in the Seine River and Estuary Using Chemical and Isotopic Techniques
journal, May 2006
- Sebilo, Mathieu; Billen, Gilles; Mayer, Bernhard
- Ecosystems, Vol. 9, Issue 4
Harmful algal blooms mitigation using clay/soil/sand modified with xanthan and calcium hydroxide
journal, November 2011
- Chen, Jing; Pan, Gang
- Journal of Applied Phycology, Vol. 24, Issue 5
Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil
journal, April 2011
- Tourna, M.; Stieglmeier, M.; Spang, A.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 20
Anammox bacteria: from discovery to application
journal, April 2008
- Kuenen, J. Gijs
- Nature Reviews Microbiology, Vol. 6, Issue 4
A Universal Method for Flocculating Harmful Algal Blooms in Marine and Fresh Waters Using Modified Sand
journal, April 2013
- Li, Liang; Pan, Gang
- Environmental Science & Technology, Vol. 47, Issue 9
Assessing carbon and nitrogen removal in a novel anoxic–aerobic cyanobacterial–bacterial photobioreactor configuration with enhanced biomass sedimentation
journal, September 2014
- de Godos, I.; Vargas, V. A.; Guzmán, H. O.
- Water Research, Vol. 61
Response of a member of the Verrucomicrobia, among the dominating bacteria in a hypolimnion, to increased phosphorus availability
journal, February 2004
- Lindstrom, E. S.
- Journal of Plankton Research, Vol. 26, Issue 2
Distribution of Typical Freshwater Bacterial Groups Is Associated with pH, Temperature, and Lake Water Retention Time
journal, December 2005
- Lindstrom, E. S.; Kamst-Van Agterveld, M. P.; Zwart, G.
- Applied and Environmental Microbiology, Vol. 71, Issue 12
A Nitrospira metagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria
journal, July 2010
- Lucker, S.; Wagner, M.; Maixner, F.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 30
A Guide to the Natural History of Freshwater Lake Bacteria
journal, March 2011
- Newton, R. J.; Jones, S. E.; Eiler, A.
- Microbiology and Molecular Biology Reviews, Vol. 75, Issue 1
Reducing the Recruitment of Sedimented Algae and Nutrient Release into the Overlying Water Using Modified Soil/Sand Flocculation-Capping in Eutrophic Lakes
journal, April 2012
- Pan, Gang; Dai, Lichun; Li, Liang
- Environmental Science & Technology, Vol. 46, Issue 9
Assignment of the genera Planctomyces and Pirella to a new family Planctomycetaceae fam. nov. and description of the order Planctomycetales ord. nov.
journal, October 1986
- Schlesner, Heinz; Stackebrandt, Erko
- Systematic and Applied Microbiology, Vol. 8, Issue 3
Application of a chitosan flocculant to water treatment
journal, January 2008
- Zeng, Defang; Wu, Juanjuan; Kennedy, John F.
- Carbohydrate Polymers, Vol. 71, Issue 1
Use of Stable-Isotope Probing, Full-Cycle rRNA Analysis, and Fluorescence In Situ Hybridization-Microautoradiography To Study a Methanol-Fed Denitrifying Microbial Community
journal, January 2004
- Ginige, M. P.; Hugenholtz, P.; Daims, H.
- Applied and Environmental Microbiology, Vol. 70, Issue 1
Effects of the novel 'Flock & Lock' lake restoration technique on Daphnia in Lake Rauwbraken (The Netherlands)
journal, August 2010
- van Oosterhout, F.; Lurling, M.
- Journal of Plankton Research, Vol. 33, Issue 2
Comparative sorption kinetic studies of ammonium onto zeolite
journal, May 2006
- Wen, D.; Ho, Y.; Tang, X.
- Journal of Hazardous Materials, Vol. 133, Issue 1-3
Effectiveness and Longevity of Phosphorus Inactivation with Alum
journal, March 1999
- Welch, Eugene B.; Cooke, G. Dennis
- Lake and Reservoir Management, Vol. 15, Issue 1
Simultaneous Removal of Harmful Algal Blooms and Microcystins Using Microorganism- and Chitosan-Modified Local Soil
journal, May 2015
- Li, Hong; Pan, Gang
- Environmental Science & Technology, Vol. 49, Issue 10
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012
- Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
- The ISME Journal, Vol. 6, Issue 8
Determination of the sedimentary microbial biomass by extractible lipid phosphate
journal, January 1979
- White, D. C.; Davis, W. M.; Nickels, J. S.
- Oecologia, Vol. 40, Issue 1
Flavobacterium johnsoniae as a Model Organism for Characterizing Biopolymer Utilization in Oligotrophic Freshwater Environments
journal, July 2011
- Sack, Eveline L. W.; van der Wielen, Paul W. J. J.; van der Kooij, Dick
- Applied and Environmental Microbiology, Vol. 77, Issue 19
A method of linking multivariate community structure to environmental variables
journal, January 1993
- Clarke, Kr; Ainsworth, M.
- Marine Ecology Progress Series, Vol. 92
Shifts in microbial community in response to dissolved oxygen levels in activated sludge
journal, August 2014
- Yadav, Trilok Chandra; Khardenavis, Anshuman A.; Kapley, Atya
- Bioresource Technology, Vol. 165
Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes
journal, March 2007
- Eiler, Alexander; Bertilsson, Stefan
- Environmental Microbiology, Vol. 9, Issue 3
Species divergence and the measurement of microbial diversity
journal, July 2008
- Lozupone, Catherine A.; Knight, Rob
- FEMS Microbiology Reviews, Vol. 32, Issue 4
phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
journal, April 2013
- McMurdie, Paul J.; Holmes, Susan
- PLoS ONE, Vol. 8, Issue 4
Enhanced phosphorus removal from sewage in mesocosm-scale constructed wetland using zeolite as medium and artificial aeration
journal, January 2014
- Vera, I.; Araya, F.; Andrés, E.
- Environmental Technology, Vol. 35, Issue 13
UPARSE: highly accurate OTU sequences from microbial amplicon reads
journal, August 2013
- Edgar, Robert C.
- Nature Methods, Vol. 10, Issue 10
Effect of Nutrient Loading on Bacterioplankton Community Composition in Lake Mesocosms
journal, January 2006
- Haukka, Kaisa; Kolmonen, Eija; Hyder, Rafiqul
- Microbial Ecology, Vol. 51, Issue 2
Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and rivers
journal, July 2009
- Hahn, M. W.; Lang, E.; Brandt, U.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 1