Clay Flocculation Effect on Microbial Community Composition in Water and Sediment

doi:10.3389/fenvs.2018.00060

Title: Clay Flocculation Effect on Microbial Community Composition in Water and Sediment

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Abstract

Clay-based flocculation techniques have been developed to mitigate harmful algal blooms; however, the potential ecological impacts on the microbial community are poorly understood. In this study, chemical measurements were combined with 16S rRNA sequencing to characterize the microbial community response to different flocculation techniques, including controls, clay flocculation, clay flocculation with zeolite, and clay flocculation with O ₂ added zeolite capping. Sediment bacterial biomass measured by PLFA were not significantly altered by the various flocculation techniques used. However, 16S rRNA sequencing revealed differences in water microbial community structure between treatments with and without zeolite capping. The differences were related to significant reductions of total nitrogen (TN), total phosphorus (TP) and ammonia (NH ₄₊) concentration and increase of nitrate (NO _3-) concentration in zeolite and O ₂ loaded zeolite capping. The relative abundance of ammonia oxidizing bacteria increased four-fold in zeolite capping microcosms, suggesting zeolite promoted absorbed ammonia removal in the benthic zone. Zeolite-capping promoted bacteria nitrogen cycling activities at the water-sediment interface. Potential pathogens that are usually adapted to eutrophic water bodies were reduced after clay flocculation. This study demonstrated clay flocculation did not decrease bacterial populations overall and may reduce regulatory indicators and pathogenic contaminants in water. Zeolite cappingmore »« less

Authors:

Chen, Chunyi ^[1]; Pan, Gang ^[2]; Shi, Wenqing ^[2]; Xu, Feng ^[2]; Techtmann, Stephen M. ^[3]; Pfiffner, Susan M. ^[4]; Hazen, Terry C. ^[4]

Univ. of Tennessee, Knoxville, TN (United States)
Chinese Academy of Sciences (CAS), Beijing (China)
Univ. of Tennessee, Knoxville, TN (United States); Michigan Technological Univ., Houghton, MI (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:: 2018-06-25

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1474524

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Frontiers in Environmental Science

Additional Journal Information:: Journal Volume: 6; Journal Issue: n/a; Journal ID: ISSN 2296-665X

Publisher:: Frontiers Research Foundation

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES

Citation Formats


                    Chen, Chunyi, Pan, Gang, Shi, Wenqing, Xu, Feng, Techtmann, Stephen M., Pfiffner, Susan M., and Hazen, Terry C. Clay Flocculation Effect on Microbial Community Composition in Water and Sediment.  United States: N. p., 2018. 
        Web.  doi:10.3389/fenvs.2018.00060.

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                    Chen, Chunyi, Pan, Gang, Shi, Wenqing, Xu, Feng, Techtmann, Stephen M., Pfiffner, Susan M., & Hazen, Terry C. Clay Flocculation Effect on Microbial Community Composition in Water and Sediment.  United States.  doi:10.3389/fenvs.2018.00060.

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                    Chen, Chunyi, Pan, Gang, Shi, Wenqing, Xu, Feng, Techtmann, Stephen M., Pfiffner, Susan M., and Hazen, Terry C. Mon .  
        "Clay Flocculation Effect on Microbial Community Composition in Water and Sediment".  United States.  doi:10.3389/fenvs.2018.00060.  https://www.osti.gov/servlets/purl/1474524.

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@article{osti_1474524,

  title        = {Clay Flocculation Effect on Microbial Community Composition in Water and Sediment},

  author       = {Chen, Chunyi and Pan, Gang and Shi, Wenqing and Xu, Feng and Techtmann, Stephen M. and Pfiffner, Susan M. and Hazen, Terry C.},

  abstractNote = {Clay-based flocculation techniques have been developed to mitigate harmful algal blooms; however, the potential ecological impacts on the microbial community are poorly understood. In this study, chemical measurements were combined with 16S rRNA sequencing to characterize the microbial community response to different flocculation techniques, including controls, clay flocculation, clay flocculation with zeolite, and clay flocculation with O2 added zeolite capping. Sediment bacterial biomass measured by PLFA were not significantly altered by the various flocculation techniques used. However, 16S rRNA sequencing revealed differences in water microbial community structure between treatments with and without zeolite capping. The differences were related to significant reductions of total nitrogen (TN), total phosphorus (TP) and ammonia (NH4+) concentration and increase of nitrate (NO3-) concentration in zeolite and O2 loaded zeolite capping. The relative abundance of ammonia oxidizing bacteria increased four-fold in zeolite capping microcosms, suggesting zeolite promoted absorbed ammonia removal in the benthic zone. Zeolite-capping promoted bacteria nitrogen cycling activities at the water-sediment interface. Potential pathogens that are usually adapted to eutrophic water bodies were reduced after clay flocculation. This study demonstrated clay flocculation did not decrease bacterial populations overall and may reduce regulatory indicators and pathogenic contaminants in water. Zeolite capping may also help prevent nutrients from being released back into the water thus preventing additional algal blooms.},

  doi          = {10.3389/fenvs.2018.00060},

  journal      = {Frontiers in Environmental Science},

  number       = n/a,

  volume       = 6,

  place        = {United States},

  year         = {2018},

  month        = {6}

}

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DOI: 10.3389/fenvs.2018.00060

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