skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization

Abstract

Agave has recently shown its potential as a bioenergy feedstock with promising features such as higher biomass productivity than leading bioenergy feedstock while at the same time being drought-resistant with low water requirements and high sugar to ethanol conversion using ionic liquid (IL) pretreatment. Here, IL pretreatment was studied to develop the first direct side-by-side comparative recalcitrance assessment of the agro-industrial residues from five Agave species [ Agave americana (AME), A. angustifolia (ANG), A. fourcroydes (FOU), A. salmiana (SAL), and A. tequilana (TEQ)] using compositional analysis, X-ray diffraction, and the lignin syringyl/guaiacyl subunit ratio (S/G) by pyrolysis molecular beam mass spectrometry (PyMBMS). Prominent calcium oxalate peaks were found only in unpretreated AME, SAL, and TEQ. The S/G ratios of all five unpretreated Agave species were between 1.27 and 1.57 while the IL-pretreated samples were from 1.39 to 1.72. The highest overall sugar production was obtained with IL-pretreated FOU with 492 mg glucose/g biomass and 157 mg xylose/g biomass at 120°C and 3 h using 1-ethyl-3-methylimidazolium acetate ([C 2C 1Im][OAc]). An estimated theoretical ethanol yield from the studied agro-industrial residues from the five Agave species was in the range of 1060 to 5800 L ethanol/ha/year. These comparison results demonstrate the potentialmore » of the Agave spp. as a suitable biofuel feedstock which can be employed within a biorefinery scheme.« less

Authors:
 [1];  [1];  [2];  [3];  [4]
  1. Autonomous Univ. of Nayarit, Tepic (Mexico). Dept. of Chemical Engineering
  2. Autonomous Univ. of Nayarit, Tepic (Mexico). Food Technology Unit
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Contributing Org.:
Novozymes A/S, Copenhagen (Denmark); Bioagaves de la Costa, Tepic (Mexico); Destilería Leyros, Tequila (Mexico); Mezcal Koch, Oaxaca (Mexico); Hacienda Aké, Tixkokob (Mexico)
OSTI Identifier:
1474460
Report Number(s):
NREL/JA-2700-71742
Journal ID: ISSN 1939-1234
Grant/Contract Number:  
AC05-00OR22725; AC36-08-GO28308
Resource Type:
Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 11; Journal Issue: 3; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Agave; Biofuels; Characterization; Crystallinity; S/G; Saccharification

Citation Formats

Pérez-Pimienta, José A., Mojica-Álvarez, Reyna M., Sánchez-Herrera, Leticia M., Mittal, Ashutosh, and Sykes, Robert W. Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization. United States: N. p., 2018. Web. doi:10.1007/s12155-018-9920-5.
Pérez-Pimienta, José A., Mojica-Álvarez, Reyna M., Sánchez-Herrera, Leticia M., Mittal, Ashutosh, & Sykes, Robert W. Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization. United States. doi:10.1007/s12155-018-9920-5.
Pérez-Pimienta, José A., Mojica-Álvarez, Reyna M., Sánchez-Herrera, Leticia M., Mittal, Ashutosh, and Sykes, Robert W. Tue . "Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization". United States. doi:10.1007/s12155-018-9920-5. https://www.osti.gov/servlets/purl/1474460.
@article{osti_1474460,
title = {Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization},
author = {Pérez-Pimienta, José A. and Mojica-Álvarez, Reyna M. and Sánchez-Herrera, Leticia M. and Mittal, Ashutosh and Sykes, Robert W.},
abstractNote = {Agave has recently shown its potential as a bioenergy feedstock with promising features such as higher biomass productivity than leading bioenergy feedstock while at the same time being drought-resistant with low water requirements and high sugar to ethanol conversion using ionic liquid (IL) pretreatment. Here, IL pretreatment was studied to develop the first direct side-by-side comparative recalcitrance assessment of the agro-industrial residues from five Agave species [Agave americana (AME), A. angustifolia (ANG), A. fourcroydes (FOU), A. salmiana (SAL), and A. tequilana (TEQ)] using compositional analysis, X-ray diffraction, and the lignin syringyl/guaiacyl subunit ratio (S/G) by pyrolysis molecular beam mass spectrometry (PyMBMS). Prominent calcium oxalate peaks were found only in unpretreated AME, SAL, and TEQ. The S/G ratios of all five unpretreated Agave species were between 1.27 and 1.57 while the IL-pretreated samples were from 1.39 to 1.72. The highest overall sugar production was obtained with IL-pretreated FOU with 492 mg glucose/g biomass and 157 mg xylose/g biomass at 120°C and 3 h using 1-ethyl-3-methylimidazolium acetate ([C2C1Im][OAc]). An estimated theoretical ethanol yield from the studied agro-industrial residues from the five Agave species was in the range of 1060 to 5800 L ethanol/ha/year. These comparison results demonstrate the potential of the Agave spp. as a suitable biofuel feedstock which can be employed within a biorefinery scheme.},
doi = {10.1007/s12155-018-9920-5},
journal = {BioEnergy Research},
number = 3,
volume = 11,
place = {United States},
year = {2018},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Biomass characterization of Agave and Opuntia as potential biofuel feedstocks
journal, May 2015


Sustainable bioethanol production combining biorefinery principles using combined raw materials from wheat undersown with clover-grass
journal, March 2008

  • Thomsen, Mette Hedegaard; Haugaard-Nielsen, Henrik
  • Journal of Industrial Microbiology & Biotechnology, Vol. 35, Issue 5
  • DOI: 10.1007/s10295-008-0334-9

Feedstocks for Lignocellulosic Biofuels
journal, August 2010

  • Somerville, Cris; Youngs, Heather; Taylor, Caroline
  • Science, Vol. 329, Issue 5993, p. 790-792
  • DOI: 10.1126/science.1189268

High annual productivity of certain agaves and cacti under cultivation
journal, April 1992


Techno-economic analysis of a lignocellulosic ethanol biorefinery with ionic liquid pre-treatment
journal, June 2011

  • Klein-Marcuschamer, Daniel; Simmons, Blake A.; Blanch, Harvey W.
  • Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 5
  • DOI: 10.1002/bbb.303

Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover
journal, July 2011


Environmental responses and productivity of the CAM plant, Agave tequilana
journal, April 1987


Wood Chemical Composition in Species of Cactaceae: The Relationship between Lignification and Stem Morphology
journal, April 2015


ORIGINAL RESEARCH: Lignocellulose recalcitrance screening by integrated high-throughput hydrothermal pretreatment and enzymatic saccharification
journal, April 2010

  • Selig, Michael J.; Tucker, Melvin P.; Sykes, Robert W.
  • Industrial Biotechnology, Vol. 6, Issue 2
  • DOI: 10.1089/ind.2010.0009

Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose
journal, August 2014

  • Socha, A. M.; Parthasarathi, R.; Shi, J.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 35
  • DOI: 10.1073/pnas.1405685111

Development of Agave as a dedicated biomass source: production of biofuels from whole plants
journal, May 2015

  • Mielenz, Jonathan R.; Rodriguez, Miguel; Thompson, Olivia A.
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0261-8

Par, Water, and Temperature Limitations on the Productivity of Cultivated Agave fourcroydes (Henequen)
journal, April 1985

  • Nobel, P. S.
  • The Journal of Applied Ecology, Vol. 22, Issue 1
  • DOI: 10.2307/2403334

Biotechnological revalorization of Tequila waste and by-product streams for cleaner production – A review from bio-refinery perspective
journal, January 2018

  • Alemán-Nava, Gibrán S.; Gatti, Ilaria Alessandra; Parra-Saldivar, Roberto
  • Journal of Cleaner Production, Vol. 172
  • DOI: 10.1016/j.jclepro.2017.07.134

Pretreatment of Agave americana stalk for enzymatic saccharification
journal, December 2012


Recent innovations in analytical methods for the qualitative and quantitative assessment of lignin
journal, September 2015

  • Lupoi, Jason S.; Singh, Seema; Parthasarathi, Ramakrishnan
  • Renewable and Sustainable Energy Reviews, Vol. 49
  • DOI: 10.1016/j.rser.2015.04.091

Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder
journal, September 2014


Life cycle energy and greenhouse gas analysis for agave-derived bioethanol
journal, January 2011

  • Yan, Xiaoyu; Tan, Daniel K. Y.; Inderwildi, Oliver R.
  • Energy & Environmental Science, Vol. 4, Issue 9
  • DOI: 10.1039/c1ee01107c

The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials
journal, January 2016


Tequila Production
journal, January 1995


Enzymatic hydrolysis at high-solids loadings for the conversion of agave bagasse to fuel ethanol
journal, January 2014


Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments
journal, July 2016


Ternary ionic liquid–water pretreatment systems of an agave bagasse and municipal solid waste blend
journal, March 2017

  • Perez-Pimienta, Jose A.; Sathitsuksanoh, Noppadon; Thompson, Vicki S.
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0758-4

UV–vis absorption spectroscopy and multivariate analysis as a method to discriminate tequila
journal, January 2007

  • Barbosa-García, O.; Ramos-Ortíz, G.; Maldonado, J. L.
  • Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 66, Issue 1
  • DOI: 10.1016/j.saa.2006.02.033

Molecular characterization of the pyrolysis of biomass
journal, March 1987


The global potential for Agave as a biofuel feedstock: AGAVE AS A BIOFUEL FEEDSTOCK
journal, November 2010


Understanding the chemical transformations of lignin during ionic liquid pretreatment
journal, January 2014

  • Wen, Jia-Long; Yuan, Tong-Qi; Sun, Shao-Long
  • Green Chem., Vol. 16, Issue 1
  • DOI: 10.1039/C3GC41752B

Sequential enzymatic saccharification and fermentation of ionic liquid and organosolv pretreated agave bagasse for ethanol production
journal, February 2017


Pretreatment of Lignocellulosic Biomass with Ionic Liquids and Ionic Liquid-Based Solvent Systems
journal, March 2017


Autohydrolysis pretreatment assessment in ethanol production from agave bagasse
journal, October 2017

  • Rios-González, Leopoldo J.; Morales-Martínez, Thelma K.; Rodríguez-Flores, María F.
  • Bioresource Technology, Vol. 242
  • DOI: 10.1016/j.biortech.2017.03.039

Long-term variability in sugarcane bagasse feedstock compositional methods: sources and magnitude of analytical variability
journal, October 2016

  • Templeton, David W.; Sluiter, Justin B.; Sluiter, Amie
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0621-z

Characterization of agave bagasse as a function of ionic liquid pretreatment
journal, April 2015


Efficient chemical and enzymatic saccharification of the lignocellulosic residue from Agave tequilana bagasse to produce ethanol by Pichia caribbica
journal, November 2010

  • Saucedo-Luna, Jaime; Castro-Montoya, Agustin Jaime; Martinez-Pacheco, Mauro Manuel
  • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 6
  • DOI: 10.1007/s10295-010-0853-z

Comparison of the impact of ionic liquid pretreatment on recalcitrance of agave bagasse and switchgrass
journal, January 2013


Isolation and Characterization of Cellulose Nanocrystals from Agave angustifolia Fibre
journal, January 2013


Technical and economical evaluation of bioethanol production from lignocellulosic residues in Mexico: Case of sugarcane and blue agave bagasses
journal, March 2016

  • Barrera, Iliana; Amezcua-Allieri, Myriam A.; Estupiñan, Lorena
  • Chemical Engineering Research and Design, Vol. 107
  • DOI: 10.1016/j.cherd.2015.10.015

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


The world availability of non-wood lignocellulosic biomass for the production of cellulosic ethanol and potential pretreatments for the enhancement of enzymatic saccharification
journal, July 2016

  • Tye, Ying Ying; Lee, Keat Teong; Wan Abdullah, Wan Nadiah
  • Renewable and Sustainable Energy Reviews, Vol. 60
  • DOI: 10.1016/j.rser.2016.01.072

Fractional pretreatment of raw and calcium oxalate-extracted agave bagasse using ionic liquid and alkaline hydrogen peroxide
journal, August 2016


An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer
journal, October 1959


Hydraulic Conductivity, Xylem Cavitation, and Water Potential for Succulent Leaves of Agave deserti and Agave tequilana
journal, July 2001

  • Linton, Matthew J.; Nobel, Park S.
  • International Journal of Plant Sciences, Vol. 162, Issue 4
  • DOI: 10.1086/320782

Physical and Chemical Characterization of Agave tequilana Bagasse Pretreated with the Ionic Liquid 1-Ethyl-3-Methylimidazolium Acetate
journal, November 2017

  • Equihua-Sánchez, Mintzirani; Barahona-Pérez, Luis F.
  • Waste and Biomass Valorization, Vol. 10, Issue 5
  • DOI: 10.1007/s12649-017-0150-4

Contribution of agro-waste material main components (hemicelluloses, cellulose, and lignin) to the removal of chromium (III) from aqueous solution
journal, June 2009

  • Garcia-Reyes, Refugio Bernardo; Rangel-Mendez, Jose Rene
  • Journal of Chemical Technology & Biotechnology, Vol. 84, Issue 10
  • DOI: 10.1002/jctb.2215

Granola bars prepared with Agave tequilana ingredients: Chemical composition and in vitro starch hydrolysis
journal, May 2014

  • Zamora-Gasga, Victor M.; Bello-Pérez, Luis A.; Ortíz-Basurto, Rosa I.
  • LWT - Food Science and Technology, Vol. 56, Issue 2
  • DOI: 10.1016/j.lwt.2013.12.016

Lignin-first biomass fractionation: the advent of active stabilisation strategies
journal, January 2017

  • Renders, T.; Van den Bosch, S.; Koelewijn, S. -F.
  • Energy & Environmental Science, Vol. 10, Issue 7
  • DOI: 10.1039/C7EE01298E

Monitoring and Analyzing Process Streams Towards Understanding Ionic Liquid Pretreatment of Switchgrass (Panicum virgatum L.)
journal, April 2010


Cellulose solvent-based biomass pretreatment breaks highly ordered hydrogen bonds in cellulose fibers of switchgrass
journal, November 2010

  • Sathitsuksanoh, Noppadon; Zhu, Zhiguang; Wi, Sungsool
  • Biotechnology and Bioengineering, Vol. 108, Issue 3
  • DOI: 10.1002/bit.22964

Two High-Throughput Techniques for Determining Wood Properties as Part of a Molecular Genetics Analysis of Hybrid Poplar and Loblolly Pine
journal, January 1999

  • Tuskan, Gerald; West, Darrell; Bradshaw, Harvy D.
  • Applied Biochemistry and Biotechnology, Vol. 77, Issue 1-3
  • DOI: 10.1385/ABAB:77:1-3:55

Efficient dehydration and recovery of ionic liquid after lignocellulosic processing using pervaporation
journal, June 2017

  • Sun, Jian; Shi, Jian; Murthy Konda, N. V. S. N.
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0842-9

Agave proves to be a low recalcitrant lignocellulosic feedstock for biofuels production on semi-arid lands
journal, January 2014

  • Li, Hongjia; Pattathil, Sivakumar; Foston, Marcus B.
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/1754-6834-7-50

Chemical composition and characterization of cellulose for Agave as a fast-growing, drought-tolerant biofuels feedstock
journal, January 2012

  • Li, Hongjia; Foston, Marcus B.; Kumar, Rajeev
  • RSC Advances, Vol. 2, Issue 11
  • DOI: 10.1039/c2ra20557b

Recent developments in Agave performance as a drought‐tolerant biofuel feedstock: agronomics, characterization, and biorefining
journal, May 2017

  • Pérez‐Pimienta, José A.; López‐Ortega, Mónica G.; Sanchez, Arturo
  • Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 4
  • DOI: 10.1002/bbb.1776