Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system
Abstract
Changes in frequency and amplitude of rain events, that is, precipitation patterns, result in different water conditions with soil depth, and likely affect plant growth and shape plant and soil microbial activity. Here, we used 18O stable isotope probing (SIP) to investigate bacterial and fungal communities that actively grew or not upon rewetting, at three different depths in soil mesocosms previously subjected to frequent or infrequent watering for 12 weeks (equal total water input). Phylogenetic marker genes for bacteria and fungi were sequenced after rewetting, and plant-soil microbial coupling documented by plant 13C-CO2 labeling. Soil depth, rather than precipitation pattern, was most influential in shaping microbial response to rewetting, and had differential effects on active and inactive bacterial and fungal communities. After rewetting, active bacterial communities were less rich, more even and phylogenetically related than the inactive, and reactivated throughout the soil profile. Active fungal communities after rewetting were less abundant and rich than the inactive. The coupling between plants and soil microbes decreased under infrequent watering in the top soil layer. In conclusion, we suggest that differences in fungal and bacterial abundance and relative activity could result in large effects on subsequent soil biogeochemical cycling.
- Authors:
-
- Univ. Bourgogne Franche-Comte, Dijon (France)
- Univ. Bourgogne Franche-Comte, Dijon (France); Iowa State Univ., Ames, IA (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Swiss Federal Research Institute WSL, Birmensdorf (Switzerland)
- Univ. Politecnica de Madrid, Madrid (Spain)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1474362
- Report Number(s):
- LLNL-JRNL-738625
Journal ID: ISSN 1751-7362; 891882
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Volume: 12; Journal ID: ISSN 1751-7362
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Engelhardt, Ilonka C., Welty, Amy, Blazewicz, Steven J., Bru, David, Rouard, Nadine, Breuil, Marie -Christine, Gessler, Arthur, Galiano, Lucía, Miranda, José Carlos, Spor, Aymé, and Barnard, Romain L. Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system. United States: N. p., 2018.
Web. doi:10.1038/s41396-018-0079-z.
Engelhardt, Ilonka C., Welty, Amy, Blazewicz, Steven J., Bru, David, Rouard, Nadine, Breuil, Marie -Christine, Gessler, Arthur, Galiano, Lucía, Miranda, José Carlos, Spor, Aymé, & Barnard, Romain L. Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system. United States. https://doi.org/10.1038/s41396-018-0079-z
Engelhardt, Ilonka C., Welty, Amy, Blazewicz, Steven J., Bru, David, Rouard, Nadine, Breuil, Marie -Christine, Gessler, Arthur, Galiano, Lucía, Miranda, José Carlos, Spor, Aymé, and Barnard, Romain L. Fri .
"Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system". United States. https://doi.org/10.1038/s41396-018-0079-z. https://www.osti.gov/servlets/purl/1474362.
@article{osti_1474362,
title = {Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system},
author = {Engelhardt, Ilonka C. and Welty, Amy and Blazewicz, Steven J. and Bru, David and Rouard, Nadine and Breuil, Marie -Christine and Gessler, Arthur and Galiano, Lucía and Miranda, José Carlos and Spor, Aymé and Barnard, Romain L.},
abstractNote = {Changes in frequency and amplitude of rain events, that is, precipitation patterns, result in different water conditions with soil depth, and likely affect plant growth and shape plant and soil microbial activity. Here, we used 18O stable isotope probing (SIP) to investigate bacterial and fungal communities that actively grew or not upon rewetting, at three different depths in soil mesocosms previously subjected to frequent or infrequent watering for 12 weeks (equal total water input). Phylogenetic marker genes for bacteria and fungi were sequenced after rewetting, and plant-soil microbial coupling documented by plant 13C-CO2 labeling. Soil depth, rather than precipitation pattern, was most influential in shaping microbial response to rewetting, and had differential effects on active and inactive bacterial and fungal communities. After rewetting, active bacterial communities were less rich, more even and phylogenetically related than the inactive, and reactivated throughout the soil profile. Active fungal communities after rewetting were less abundant and rich than the inactive. The coupling between plants and soil microbes decreased under infrequent watering in the top soil layer. In conclusion, we suggest that differences in fungal and bacterial abundance and relative activity could result in large effects on subsequent soil biogeochemical cycling.},
doi = {10.1038/s41396-018-0079-z},
journal = {The ISME Journal},
number = ,
volume = 12,
place = {United States},
year = {2018},
month = {2}
}
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Figures / Tables:
Fig. 1: Dynamics of soil water content in the experimental treatments (infrequent and frequent water input, dotted and full curves, respectively) over the duration of the experiment. Lines and shaded polygons around them indicate mean ± standard error (n=5).
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Works referencing / citing this record:
Soil microbial moisture dependences and responses to drying–rewetting: The legacy of 18 years drought
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Rapid Shifts in Bacterial Community Assembly under Static and Dynamic Hydration Conditions in Porous Media
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Contrasting Biogeographic Patterns of Bacterial and Archaeal Diversity in the Top- and Subsoils of Temperate Grasslands
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Rapid Shifts in Bacterial Community Assembly under Static and Dynamic Hydration Conditions in Porous Media
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