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Title: Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system

Changes in frequency and amplitude of rain events, that is, precipitation patterns, result in different water conditions with soil depth, and likely affect plant growth and shape plant and soil microbial activity. Here, we used 18O stable isotope probing (SIP) to investigate bacterial and fungal communities that actively grew or not upon rewetting, at three different depths in soil mesocosms previously subjected to frequent or infrequent watering for 12 weeks (equal total water input). Phylogenetic marker genes for bacteria and fungi were sequenced after rewetting, and plant-soil microbial coupling documented by plant 13C-CO 2 labeling. Soil depth, rather than precipitation pattern, was most influential in shaping microbial response to rewetting, and had differential effects on active and inactive bacterial and fungal communities. After rewetting, active bacterial communities were less rich, more even and phylogenetically related than the inactive, and reactivated throughout the soil profile. Active fungal communities after rewetting were less abundant and rich than the inactive. The coupling between plants and soil microbes decreased under infrequent watering in the top soil layer. In conclusion, we suggest that differences in fungal and bacterial abundance and relative activity could result in large effects on subsequent soil biogeochemical cycling.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [1] ;  [1] ;  [4] ;  [4] ;  [5] ;  [1] ;  [1]
  1. Univ. Bourgogne Franche-Comte, Dijon (France)
  2. Univ. Bourgogne Franche-Comte, Dijon (France); Iowa State Univ., Ames, IA (United States)
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  4. Swiss Federal Research Institute WSL, Birmensdorf (Switzerland)
  5. Univ. Politecnica de Madrid, Madrid (Spain)
Publication Date:
Report Number(s):
LLNL-JRNL-738625
Journal ID: ISSN 1751-7362; 891882
Grant/Contract Number:
AC52-07NA27344
Type:
Accepted Manuscript
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Volume: 12; Journal ID: ISSN 1751-7362
Publisher:
Nature Publishing Group
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
USDOE National Nuclear Security Administration (NNSA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1474362

Engelhardt, Ilonka C., Welty, Amy, Blazewicz, Steven J., Bru, David, Rouard, Nadine, Breuil, Marie -Christine, Gessler, Arthur, Galiano, Lucía, Miranda, José Carlos, Spor, Aymé, and Barnard, Romain L.. Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system. United States: N. p., Web. doi:10.1038/s41396-018-0079-z.
Engelhardt, Ilonka C., Welty, Amy, Blazewicz, Steven J., Bru, David, Rouard, Nadine, Breuil, Marie -Christine, Gessler, Arthur, Galiano, Lucía, Miranda, José Carlos, Spor, Aymé, & Barnard, Romain L.. Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system. United States. doi:10.1038/s41396-018-0079-z.
Engelhardt, Ilonka C., Welty, Amy, Blazewicz, Steven J., Bru, David, Rouard, Nadine, Breuil, Marie -Christine, Gessler, Arthur, Galiano, Lucía, Miranda, José Carlos, Spor, Aymé, and Barnard, Romain L.. 2018. "Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system". United States. doi:10.1038/s41396-018-0079-z.
@article{osti_1474362,
title = {Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system},
author = {Engelhardt, Ilonka C. and Welty, Amy and Blazewicz, Steven J. and Bru, David and Rouard, Nadine and Breuil, Marie -Christine and Gessler, Arthur and Galiano, Lucía and Miranda, José Carlos and Spor, Aymé and Barnard, Romain L.},
abstractNote = {Changes in frequency and amplitude of rain events, that is, precipitation patterns, result in different water conditions with soil depth, and likely affect plant growth and shape plant and soil microbial activity. Here, we used 18O stable isotope probing (SIP) to investigate bacterial and fungal communities that actively grew or not upon rewetting, at three different depths in soil mesocosms previously subjected to frequent or infrequent watering for 12 weeks (equal total water input). Phylogenetic marker genes for bacteria and fungi were sequenced after rewetting, and plant-soil microbial coupling documented by plant 13C-CO2 labeling. Soil depth, rather than precipitation pattern, was most influential in shaping microbial response to rewetting, and had differential effects on active and inactive bacterial and fungal communities. After rewetting, active bacterial communities were less rich, more even and phylogenetically related than the inactive, and reactivated throughout the soil profile. Active fungal communities after rewetting were less abundant and rich than the inactive. The coupling between plants and soil microbes decreased under infrequent watering in the top soil layer. In conclusion, we suggest that differences in fungal and bacterial abundance and relative activity could result in large effects on subsequent soil biogeochemical cycling.},
doi = {10.1038/s41396-018-0079-z},
journal = {The ISME Journal},
number = ,
volume = 12,
place = {United States},
year = {2018},
month = {2}
}

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