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Title: Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells

Lipid-coated mesoporous silica nanoparticles (LC-MSNs) have recently emerged as a next-generation cargo delivery nanosystem combining the unique attributes of both the organic and inorganic components. The high surface area biodegradable inorganic mesoporous silica core can accommodate multiple classes of bio-relevant cargos in large amounts, while the supported lipid bilayer coating retains the cargo and increases the stability of the nanocarrier in bio-relevant media which should promote greater bio-accumulation of LC-MSNs in cancer sites. In this paper, we report on the optimization of various sol–gel synthesis (pH, stirring speed) and post-synthesis (hydrothermal treatment) procedures to enlarge the MSN pore size and tune the surface chemistry so as to enable loading and delivery of large biomolecules. Finally, the proof of concept of the dual cargo-loaded nanocarrier has been demonstrated in immortalized cervical cancer HeLa cells using MSNs of various fine-tuned pore sizes.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2]
  1. Univ. of New Mexico, Albuquerque, NM (United States). Chemical and Biological Engineering
  2. Univ. of New Mexico, Albuquerque, NM (United States). Chemical and Biological Engineering. Center for Micro-Engineered Materials. Advanced Materials Lab.; Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Report Number(s):
SAND-2018-10253J
Journal ID: ISSN 0928-0707; 667988
Grant/Contract Number:
NA0003525
Type:
Accepted Manuscript
Journal Name:
Journal of Sol-Gel Science and Technology
Additional Journal Information:
Journal Name: Journal of Sol-Gel Science and Technology; Journal ID: ISSN 0928-0707
Publisher:
Springer
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
Sponsoring Org:
USDOE National Nuclear Security Administration (NNSA); SNL Laboratory Directed Research and Development (LDRD) Program; Leukemia & Lymphoma Society (LLS) (United States)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; mesoporous silica nanoparticles; large pore; sol–gel; supported lipid bilayer; drug delivery; biomedical
OSTI Identifier:
1474081

Noureddine, Achraf, Hjelvik, Elizabeth A., Croissant, Jonas G., Durfee, Paul N., Agola, Jacob O., and Brinker, C. Jeffrey. Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells. United States: N. p., Web. doi:10.1007/s10971-018-4772-1.
Noureddine, Achraf, Hjelvik, Elizabeth A., Croissant, Jonas G., Durfee, Paul N., Agola, Jacob O., & Brinker, C. Jeffrey. Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells. United States. doi:10.1007/s10971-018-4772-1.
Noureddine, Achraf, Hjelvik, Elizabeth A., Croissant, Jonas G., Durfee, Paul N., Agola, Jacob O., and Brinker, C. Jeffrey. 2018. "Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells". United States. doi:10.1007/s10971-018-4772-1.
@article{osti_1474081,
title = {Engineering of large-pore lipid-coated mesoporous silica nanoparticles for dual cargo delivery to cancer cells},
author = {Noureddine, Achraf and Hjelvik, Elizabeth A. and Croissant, Jonas G. and Durfee, Paul N. and Agola, Jacob O. and Brinker, C. Jeffrey},
abstractNote = {Lipid-coated mesoporous silica nanoparticles (LC-MSNs) have recently emerged as a next-generation cargo delivery nanosystem combining the unique attributes of both the organic and inorganic components. The high surface area biodegradable inorganic mesoporous silica core can accommodate multiple classes of bio-relevant cargos in large amounts, while the supported lipid bilayer coating retains the cargo and increases the stability of the nanocarrier in bio-relevant media which should promote greater bio-accumulation of LC-MSNs in cancer sites. In this paper, we report on the optimization of various sol–gel synthesis (pH, stirring speed) and post-synthesis (hydrothermal treatment) procedures to enlarge the MSN pore size and tune the surface chemistry so as to enable loading and delivery of large biomolecules. Finally, the proof of concept of the dual cargo-loaded nanocarrier has been demonstrated in immortalized cervical cancer HeLa cells using MSNs of various fine-tuned pore sizes.},
doi = {10.1007/s10971-018-4772-1},
journal = {Journal of Sol-Gel Science and Technology},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {8}
}

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