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Title: Probing the remarkable thermal kinetics of visual rhodopsin with E181Q and S186A mutants

Abstract

We recently reported a very unusual temperature dependence of the rate of thermal reaction of wild type bovine rhodopsin: the Arrhenius plot exhibits a sharp “elbow” at 47 °C and, in the upper temperature range, an unexpectedly large activation energy (114 ± 8 kcal/mol) and an enormous prefactor (1072±5 s-1). In this report, we present new measurements and a theoretical model that establish convincingly that this behavior results from a collective, entropy-driven breakup of the rigid hydrogen bonding networks (HBNs) that hinder the reaction at lower temperatures. For E181Q and S186A, two rhodopsin mutants that disrupt the HBNs near the binding pocket of the 11-cis retinyl chromophore, we observe significant decreases in the activation energy (~90 kcal/mol) and prefactor (~1060 s-1), consistent with the conclusion that the reaction rate is enhanced by breakup of the HBN. Finally, the results provide insights into the molecular mechanism of dim-light vision and eye diseases caused by inherited mutations in the rhodopsin gene that perturb the HBNs.

Authors:
 [1]; ORCiD logo [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
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  1. Yale Univ., New Haven, CT (United States). Dept. of Chemistry
Publication Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
OSTI Identifier:
1474078
Alternate Identifier(s):
OSTI ID: 1365428
Grant/Contract Number:  
FG02-05ER15677; MCB-0955407; CHE 1465108
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Chemical Physics
Additional Journal Information:
Journal Volume: 146; Journal Issue: 21; Journal ID: ISSN 0021-9606
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Guo, Ying, Hendrickson, Heidi P., Videla, Pablo E., Chen, Ya-Na, Ho, Junming, Sekharan, Sivakumar, Batista, Victor S., Tully, John C., and Yan, Elsa C. Y. Probing the remarkable thermal kinetics of visual rhodopsin with E181Q and S186A mutants. United States: N. p., 2017. Web. doi:10.1063/1.4984818.
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Guo, Ying, Hendrickson, Heidi P., Videla, Pablo E., Chen, Ya-Na, Ho, Junming, Sekharan, Sivakumar, Batista, Victor S., Tully, John C., & Yan, Elsa C. Y. Probing the remarkable thermal kinetics of visual rhodopsin with E181Q and S186A mutants. United States. https://doi.org/10.1063/1.4984818
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Guo, Ying, Hendrickson, Heidi P., Videla, Pablo E., Chen, Ya-Na, Ho, Junming, Sekharan, Sivakumar, Batista, Victor S., Tully, John C., and Yan, Elsa C. Y. Wed . "Probing the remarkable thermal kinetics of visual rhodopsin with E181Q and S186A mutants". United States. https://doi.org/10.1063/1.4984818. https://www.osti.gov/servlets/purl/1474078.
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@article{osti_1474078,
title = {Probing the remarkable thermal kinetics of visual rhodopsin with E181Q and S186A mutants},
author = {Guo, Ying and Hendrickson, Heidi P. and Videla, Pablo E. and Chen, Ya-Na and Ho, Junming and Sekharan, Sivakumar and Batista, Victor S. and Tully, John C. and Yan, Elsa C. Y.},
abstractNote = {We recently reported a very unusual temperature dependence of the rate of thermal reaction of wild type bovine rhodopsin: the Arrhenius plot exhibits a sharp “elbow” at 47 °C and, in the upper temperature range, an unexpectedly large activation energy (114 ± 8 kcal/mol) and an enormous prefactor (1072±5 s-1). In this report, we present new measurements and a theoretical model that establish convincingly that this behavior results from a collective, entropy-driven breakup of the rigid hydrogen bonding networks (HBNs) that hinder the reaction at lower temperatures. For E181Q and S186A, two rhodopsin mutants that disrupt the HBNs near the binding pocket of the 11-cis retinyl chromophore, we observe significant decreases in the activation energy (~90 kcal/mol) and prefactor (~1060 s-1), consistent with the conclusion that the reaction rate is enhanced by breakup of the HBN. Finally, the results provide insights into the molecular mechanism of dim-light vision and eye diseases caused by inherited mutations in the rhodopsin gene that perturb the HBNs.},
doi = {10.1063/1.4984818},
journal = {Journal of Chemical Physics},
number = 21,
volume = 146,
place = {United States},
year = {Wed Jun 07 00:00:00 EDT 2017},
month = {Wed Jun 07 00:00:00 EDT 2017}
}
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    Works referencing / citing this record:

    The counterion–retinylidene Schiff base interaction of an invertebrate rhodopsin rearranges upon light activation
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