Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters

Fernandes-Cunha, Gabriella M.; McKinlay, Colin J.; Vargas, Jessica R.; Jessen, Henning J.; Waymouth, Robert M.; Wender, Paul A.

doi:10.1021/acscentsci.8b00470

Title: Delivery of Inorganic Polyphosphate into Cells Using Amphipathic Oligocarbonate Transporters

Journal Article · 26 September 2018 · ACS Central Science

DOI: https://doi.org/10.1021/acscentsci.8b00470 · OSTI ID:1474016

^[1]; McKinlay, Colin J. ^[1]; Vargas, Jessica R. ^[1];

^[2];

^[1];

^[1]

Stanford Univ., Stanford, CA (United States)
Albert-Ludwigs Univ., Freiburg (Germany)

Inorganic polyphosphate (polyP) is an often-overlooked biopolymer of phosphate residues present in living cells. PolyP is associated with many essential biological roles. Despite interest in polyP’s function, most studies have been limited to extracellular or isolated protein experiments, as polyanionic polyP does not traverse the nonpolar membrane of cells. To address this problem, we developed a robust, readily employed method for polyP delivery using guanidinium-rich oligocarbonate transporters that electrostatically complex polyPs of multiple lengths, forming discrete nanoparticles that are resistant to phosphatase degradation and that readily enter multiple cell types. Fluorescently labeled polyPs have been monitored over time for subcellular localization and release from the transporter, with control over release rates achieved by modulating the transporter identity and the charge ratio of the electrostatic complexes. Furthermore, this general approach to polyP delivery enables the study of intracellular polyP signaling in a variety of applications.

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Research Organization:: Leland Stanford Junior Univ., Palo Alto, CA (United States); Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0018168

OSTI ID:: 1474016

Alternate ID(s):: OSTI ID: 1483936; OSTI ID: 1508740

Journal Information:: ACS Central Science, Vol. 4, Issue 10; ISSN 2374-7943

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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Inorganic polyphosphate stimulates mammalian TOR, a kinase involved in the proliferation of mammary cancer cells Wang, L.; Fraley, C. D.; Faridi, J. Proceedings of the National Academy of Sciences, Vol. 100, Issue 20 https://doi.org/10.1073/pnas.1534805100	journal	September 2003
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Two new protocols to enhance the production and isolation of human induced pluripotent stem cell lines Dick, Emily; Matsa, Elena; Bispham, Jayson Stem Cell Research, Vol. 6, Issue 2 https://doi.org/10.1016/j.scr.2010.10.002	journal	March 2011
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Targeted polyphosphatase expression alters mitochondrial metabolism and inhibits calcium-dependent cell death Abramov, A. Y.; Fraley, C.; Diao, C. T. Proceedings of the National Academy of Sciences, Vol. 104, Issue 46 https://doi.org/10.1073/pnas.0708959104	journal	November 2007
Designed guanidinium-rich amphipathic oligocarbonate molecular transporters complex, deliver and release siRNA in cells Geihe, E. I.; Cooley, C. B.; Simon, J. R. Proceedings of the National Academy of Sciences, Vol. 109, Issue 33 https://doi.org/10.1073/pnas.1211361109	journal	July 2012
Inorganic polyphosphate stimulates mammalian TOR, a kinase involved in the proliferation of mammary cancer cells Wang, L.; Fraley, C. D.; Faridi, J. Proceedings of the National Academy of Sciences, Vol. 100, Issue 20 https://doi.org/10.1073/pnas.1534805100	journal	September 2003
Charge-altering releasable transporters (CARTs) for the delivery and release of mRNA in living animals McKinlay, Colin J.; Vargas, Jessica R.; Blake, Timothy R. Proceedings of the National Academy of Sciences, Vol. 114, Issue 4 https://doi.org/10.1073/pnas.1614193114	journal	January 2017
Enhanced mRNA delivery into lymphocytes enabled by lipid-varied libraries of charge-altering releasable transporters McKinlay, Colin J.; Benner, Nancy L.; Haabeth, Ole A. Proceedings of the National Academy of Sciences, Vol. 115, Issue 26 https://doi.org/10.1073/pnas.1805358115	journal	June 2018
The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: Peptoid molecular transporters Wender, P. A.; Mitchell, D. J.; Pattabiraman, K. Proceedings of the National Academy of Sciences, Vol. 97, Issue 24, p. 13003-13008 https://doi.org/10.1073/pnas.97.24.13003	journal	November 2000
Inorganic polyphosphate is a potent activator of the mitochondrial permeability transition pore in cardiac myocytes Seidlmayer, Lea K.; Gomez-Garcia, Maria R.; Blatter, Lothar A. Journal of General Physiology, Vol. 139, Issue 5 https://doi.org/10.1085/jgp.201210788	journal	April 2012
Drug evaluation in cardiomyocytes derived from human induced pluripotent stem cells carrying a long QT syndrome type 2 mutation Matsa, Elena; Rajamohan, Divya; Dick, Emily European Heart Journal, Vol. 32, Issue 8 https://doi.org/10.1093/eurheartj/ehr073	journal	March 2011
Inorganic polyphosphate elicits pro-inflammatory responses through activation of the mammalian target of rapamycin complexes 1 and 2 in vascular endothelial cells Hassanian, S. M.; Dinarvand, P.; Smith, S. A. Journal of Thrombosis and Haemostasis, Vol. 13, Issue 5 https://doi.org/10.1111/jth.12899	journal	April 2015
Inorganic Polyphosphate: A Molecule of Many Functions Kornberg, Arthur; Rao, Narayana N.; Ault-Riché, Dana Annual Review of Biochemistry, Vol. 68, Issue 1 https://doi.org/10.1146/annurev.biochem.68.1.89	journal	June 1999
Induction of Calcification in MC3T3-E1 Cells by Inorganic Polyphosphate Kawazoe, Y.; Shiba, T.; Nakamura, R. Journal of Dental Research, Vol. 83, Issue 8 https://doi.org/10.1177/154405910408300806	journal	August 2004
Polyphosphate: an ancient molecule that links platelets, coagulation, and inflammation Morrissey, James H.; Choi, Sharon H.; Smith, Stephanie A. Blood, Vol. 119, Issue 25 https://doi.org/10.1182/blood-2012-03-306605	journal	June 2012
Amorphous, Smart, and Bioinspired Polyphosphate Nano/Microparticles: A Biomaterial for Regeneration and Repair of Osteo-Articular Impairments In-Situ Müller, Werner; Neufurth, Meik; Wang, Shunfeng International Journal of Molecular Sciences, Vol. 19, Issue 2 https://doi.org/10.3390/ijms19020427	journal	January 2018

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