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Title: Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization

Engineering strategies to create promoters that are both higher strength and tunable in the presence of inexpensive compounds are of high importance to develop metabolic engineering technologies that can be commercialized. Lignocellulosic biomass stands out as the most abundant renewable feedstock for the production of biofuels and chemicals. However, lignin a major polymeric component of the biomass is made up of aromatic units and remains as an untapped resource. Novel synthetic biology tools for the expression of heterologous proteins are critical for the effective engineering of a microbe to valorize lignin. Furthermore, this study demonstrates the first successful attempt in the creation of engineered promoters that can be induced by aromatics present in lignocellulosic hydrolysates to increase heterologous protein production.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [3]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Arizona State Univ., Tempe, AZ (United States)
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States); Joint Bioenergy Institute, Emeryville, CA (United States); Univ. of Minnesota, St. Paul, MN (United States)
Publication Date:
Report Number(s):
SAND-2018-9919J
Journal ID: ISSN 1754-6834; 667754
Grant/Contract Number:
AC04-94AL85000
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Lignin valorization; Promoter engineering; Biochemicals; Hybrid promoters; Phenolic inducers
OSTI Identifier:
1473958

Varman, Arul M., Follenfant, Rhiannon, Liu, Fang, Davis, Ryan W., Lin, Yone K., and Singh, Seema. Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization. United States: N. p., Web. doi:10.1186/s13068-018-1179-8.
Varman, Arul M., Follenfant, Rhiannon, Liu, Fang, Davis, Ryan W., Lin, Yone K., & Singh, Seema. Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization. United States. doi:10.1186/s13068-018-1179-8.
Varman, Arul M., Follenfant, Rhiannon, Liu, Fang, Davis, Ryan W., Lin, Yone K., and Singh, Seema. 2018. "Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization". United States. doi:10.1186/s13068-018-1179-8. https://www.osti.gov/servlets/purl/1473958.
@article{osti_1473958,
title = {Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization},
author = {Varman, Arul M. and Follenfant, Rhiannon and Liu, Fang and Davis, Ryan W. and Lin, Yone K. and Singh, Seema},
abstractNote = {Engineering strategies to create promoters that are both higher strength and tunable in the presence of inexpensive compounds are of high importance to develop metabolic engineering technologies that can be commercialized. Lignocellulosic biomass stands out as the most abundant renewable feedstock for the production of biofuels and chemicals. However, lignin a major polymeric component of the biomass is made up of aromatic units and remains as an untapped resource. Novel synthetic biology tools for the expression of heterologous proteins are critical for the effective engineering of a microbe to valorize lignin. Furthermore, this study demonstrates the first successful attempt in the creation of engineered promoters that can be induced by aromatics present in lignocellulosic hydrolysates to increase heterologous protein production.},
doi = {10.1186/s13068-018-1179-8},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {United States},
year = {2018},
month = {6}
}

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