Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization

Varman, Arul M.; Follenfant, Rhiannon; Liu, Fang; Davis, Ryan W.; Lin, Yone K.; Singh, Seema

doi:10.1186/s13068-018-1179-8

Title: Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization

Journal Article · 27 June 2018 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-018-1179-8 · OSTI ID:1473958

Varman, Arul M. ^[1]; Follenfant, Rhiannon ^[2]; Liu, Fang ^[2]; Davis, Ryan W. ^[2]; Lin, Yone K. ^[2]; Singh, Seema ^[3]

Sandia National Lab. (SNL-CA), Livermore, CA (United States); Arizona State Univ., Tempe, AZ (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Joint Bioenergy Institute, Emeryville, CA (United States); Univ. of Minnesota, St. Paul, MN (United States)

Engineering strategies to create promoters that are both higher strength and tunable in the presence of inexpensive compounds are of high importance to develop metabolic engineering technologies that can be commercialized. Lignocellulosic biomass stands out as the most abundant renewable feedstock for the production of biofuels and chemicals. However, lignin a major polymeric component of the biomass is made up of aromatic units and remains as an untapped resource. Novel synthetic biology tools for the expression of heterologous proteins are critical for the effective engineering of a microbe to valorize lignin. Furthermore, this study demonstrates the first successful attempt in the creation of engineered promoters that can be induced by aromatics present in lignocellulosic hydrolysates to increase heterologous protein production.

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Research Organization:: Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1473958

Alternate ID(s):: OSTI ID: 1332660; OSTI ID: 1618726

Report Number(s):: SAND--2018-9919J; 667754

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 11; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization [Supplemental Data] Varman, Arul; Follenfant, Rhiannon; Liu, Fang https://doi.org/10.6084/M9.FIGSHARE.C.4149389 The current record is supplemented by this figure	figure	June 2018
Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization [Supplemental Data] Varman, Arul; Follenfant, Rhiannon; Liu, Fang https://doi.org/10.6084/m9.figshare.c.4149389.v1 The current record is supplemented by this figure	figure	June 2018
MOESM1 of Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization Varman, Arul; Follenfant, Rhiannon; Liu, Fang https://doi.org/10.6084/m9.figshare.6722135.v1 The current record is supplemented by this figure	figure	June 2018
MOESM1 of Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization Varman, Arul; Follenfant, Rhiannon; Liu, Fang https://doi.org/10.6084/m9.figshare.6722135 The current record is supplemented by this figure	figure	June 2018