skip to main content

DOE PAGESDOE PAGES

Title: Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll $$\mathcal{f}$$

Far-Red Light (FRL) acclimation is a process that has been observed in cyanobacteria and algae that can grow solely on light above 700 nm. The acclimation to FRL results in rearrangement and synthesis of new pigments and pigment-protein complexes. Here, cyanobacteria containing chlorophyll $$\mathcal{f}$$, Synechococcus sp. PCC 7335 and Halomicronema hongdechloris, were imaged as live cells with confocal microscopy. H. hongdechloris was further studied with hyperspectral confocal fluorescence microscopy (HCFM) and freeze-substituted thin-section transmission electron microscopy (TEM). Under FRL, phycocyanin-containing complexes and chlorophyll-containing complexes were determined to be physically separated and the synthesis of red-form phycobilisome and Chl $$\mathcal{f}$$ was increased. The timing of these responses was observed. The heterogeneity and eco-physiological response of the cells was noted. Additionally, a gliding motility for H. hongdechloris is reported.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [2] ; ORCiD logo [3] ; ORCiD logo [4] ; ORCiD logo [1]
  1. Washington Univ., St. Louis, MO (United States). Dept. of Chemistry and Biology
  2. Donald Danforth Plant Science Center, St. Louis, MO (United States)
  3. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Bioenergy and Defense Technologies Dept.
  4. Univ. of Sydney, NSW (Australia). School of Life and Environmental Sciences
Publication Date:
Report Number(s):
SAND2018-9774J
Journal ID: ISSN 0166-8595; 667651
Grant/Contract Number:
AC04-94AL85000; SC0001035; NA0003525
Type:
Accepted Manuscript
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 134; Journal Issue: 2; Journal ID: ISSN 0166-8595
Publisher:
Springer
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Washington Univ., St. Louis, MO (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE National Nuclear Security Administration (NNSA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; Far-red light acclimation; Cyanobacteria; Chlorophyll f; Phycobilisome
OSTI Identifier:
1472251

Majumder, Erica L. -W., Wolf, Benjamin M., Liu, Haijun, Berg, R. Howard, Timlin, Jerilyn A., Chen, Min, and Blankenship, Robert E.. Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll $\mathcal{f}$. United States: N. p., Web. doi:10.1007/s11120-017-0428-1.
Majumder, Erica L. -W., Wolf, Benjamin M., Liu, Haijun, Berg, R. Howard, Timlin, Jerilyn A., Chen, Min, & Blankenship, Robert E.. Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll $\mathcal{f}$. United States. doi:10.1007/s11120-017-0428-1.
Majumder, Erica L. -W., Wolf, Benjamin M., Liu, Haijun, Berg, R. Howard, Timlin, Jerilyn A., Chen, Min, and Blankenship, Robert E.. 2017. "Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll $\mathcal{f}$". United States. doi:10.1007/s11120-017-0428-1. https://www.osti.gov/servlets/purl/1472251.
@article{osti_1472251,
title = {Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll $\mathcal{f}$},
author = {Majumder, Erica L. -W. and Wolf, Benjamin M. and Liu, Haijun and Berg, R. Howard and Timlin, Jerilyn A. and Chen, Min and Blankenship, Robert E.},
abstractNote = {Far-Red Light (FRL) acclimation is a process that has been observed in cyanobacteria and algae that can grow solely on light above 700 nm. The acclimation to FRL results in rearrangement and synthesis of new pigments and pigment-protein complexes. Here, cyanobacteria containing chlorophyll $\mathcal{f}$,Synechococcus sp. PCC 7335 and Halomicronema hongdechloris, were imaged as live cells with confocal microscopy. H. hongdechloris was further studied with hyperspectral confocal fluorescence microscopy (HCFM) and freeze-substituted thin-section transmission electron microscopy (TEM). Under FRL, phycocyanin-containing complexes and chlorophyll-containing complexes were determined to be physically separated and the synthesis of red-form phycobilisome and Chl $\mathcal{f}$ was increased. The timing of these responses was observed. The heterogeneity and eco-physiological response of the cells was noted. Additionally, a gliding motility for H. hongdechloris is reported.},
doi = {10.1007/s11120-017-0428-1},
journal = {Photosynthesis Research},
number = 2,
volume = 134,
place = {United States},
year = {2017},
month = {9}
}

Works referenced in this record:

Preprocessing strategies to improve MCR analyses of hyperspectral images
journal, August 2012
  • Jones, Howland D.T.; Haaland, David M.; Sinclair, Michael B.
  • Chemometrics and Intelligent Laboratory Systems, Vol. 117, p. 149-158
  • DOI: 10.1016/j.chemolab.2012.01.011

In vivo hyperspectral confocal fluorescence imaging to determine pigment localization and distribution in cyanobacterial cells
journal, March 2008
  • Vermaas, W. F. J.; Timlin, J. A.; Jones, H. D. T.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 10, p. 4050-4055
  • DOI: 10.1073/pnas.0708090105

Hyperspectral confocal microscope
journal, January 2006
  • Sinclair, Michael B.; Haaland, David M.; Timlin, Jerilyn A.
  • Applied Optics, Vol. 45, Issue 24, p. 6283-6291
  • DOI: 10.1364/AO.45.006283

Advances in ultrarapid freezing for the preservation of cellular ultrastructure
journal, January 1986
  • Gilkey, John C.; Staehelin, L. Andrew
  • Journal of Electron Microscopy Technique, Vol. 3, Issue 2, p. 177-210
  • DOI: 10.1002/jemt.1060030206