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Title: Trehalose 6-phosphate positively regulates fatty acid synthesis by stabilizing WRINKLED1

Abstract

WRINKLED1 (WRI1), the transcriptional activator of fatty acid synthesis, was recently identified as a target of KIN10, a catalytic α-subunit of the SUCROSE-NON-FERMENTING-1-RELATED PROTEIN KINASE-1 (SnRK1). For this work, we tested the hypothesis that trehalose 6-phosphate (T6P), a signal of cellular sucrose status, can regulate FA synthesis by inhibiting SnRK1. Incubation of Brassica napus suspension cells in medium containing T6P, or overexpression of the Escherichia coli T6P synthase, OtsA, in tobacco, significantly increased T6P levels, WRI1 levels and FA synthesis rates. T6P directly bound to purified recombinant KIN10 with an equilibrium dissociation constant ( Kd) of 32 ± 6 μM using microscale thermophoresis. GEMINIVIRUS REP-INTERACTING KINASE1 (GRIK1) bound to KIN10 ( Kd 19 ± 3 μM) and activated it by phosphorylation. In the presence of T6P the GRIK1-KIN10 association was weakened by more than threefold ( Kd 67.7 ± 9.8 μM) which reduced both the phosphorylation of KIN10 and its activity. T6P-dependent inhibition of SnRK1 activity is reduced in extracts of individual Arabidopsis grik1 and grik2 mutants relative to wild type, while SnRK1 activity in grik1grik2 extracts is enhanced by T6P. These results indicate the T6P sensitivity of SnRK1 in vivo is GRIK1/GRIK2-dependent. A mechanistic model is proposed that linksmore » sugar signaling and FA homeostasis.« less

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Biology
  2. Max Planck Inst. of Molecular Plant Physiology, Potsdam (Germany)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1472222
Alternate Identifier(s):
OSTI ID: 1467850
Report Number(s):
BNL-209015-2018-JAAM
Journal ID: ISSN 1040-4651
Grant/Contract Number:  
SC0012704
Resource Type:
Published Article
Journal Name:
Plant Cell
Additional Journal Information:
Journal Volume: 30; Journal Issue: 10; Journal ID: ISSN 1040-4651
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhai, Zhiyang, Keereetaweep, Jantana, Liu, Hui, Feil, Regina, Lunn, John E., and Shanklin, John. Trehalose 6-phosphate positively regulates fatty acid synthesis by stabilizing WRINKLED1. United States: N. p., 2018. Web. doi:10.1105/tpc.18.00521.
Zhai, Zhiyang, Keereetaweep, Jantana, Liu, Hui, Feil, Regina, Lunn, John E., & Shanklin, John. Trehalose 6-phosphate positively regulates fatty acid synthesis by stabilizing WRINKLED1. United States. doi:10.1105/tpc.18.00521.
Zhai, Zhiyang, Keereetaweep, Jantana, Liu, Hui, Feil, Regina, Lunn, John E., and Shanklin, John. Mon . "Trehalose 6-phosphate positively regulates fatty acid synthesis by stabilizing WRINKLED1". United States. doi:10.1105/tpc.18.00521.
@article{osti_1472222,
title = {Trehalose 6-phosphate positively regulates fatty acid synthesis by stabilizing WRINKLED1},
author = {Zhai, Zhiyang and Keereetaweep, Jantana and Liu, Hui and Feil, Regina and Lunn, John E. and Shanklin, John},
abstractNote = {WRINKLED1 (WRI1), the transcriptional activator of fatty acid synthesis, was recently identified as a target of KIN10, a catalytic α-subunit of the SUCROSE-NON-FERMENTING-1-RELATED PROTEIN KINASE-1 (SnRK1). For this work, we tested the hypothesis that trehalose 6-phosphate (T6P), a signal of cellular sucrose status, can regulate FA synthesis by inhibiting SnRK1. Incubation of Brassica napus suspension cells in medium containing T6P, or overexpression of the Escherichia coli T6P synthase, OtsA, in tobacco, significantly increased T6P levels, WRI1 levels and FA synthesis rates. T6P directly bound to purified recombinant KIN10 with an equilibrium dissociation constant (Kd) of 32 ± 6 μM using microscale thermophoresis. GEMINIVIRUS REP-INTERACTING KINASE1 (GRIK1) bound to KIN10 (Kd 19 ± 3 μM) and activated it by phosphorylation. In the presence of T6P the GRIK1-KIN10 association was weakened by more than threefold (Kd 67.7 ± 9.8 μM) which reduced both the phosphorylation of KIN10 and its activity. T6P-dependent inhibition of SnRK1 activity is reduced in extracts of individual Arabidopsis grik1 and grik2 mutants relative to wild type, while SnRK1 activity in grik1grik2 extracts is enhanced by T6P. These results indicate the T6P sensitivity of SnRK1 in vivo is GRIK1/GRIK2-dependent. A mechanistic model is proposed that links sugar signaling and FA homeostasis.},
doi = {10.1105/tpc.18.00521},
journal = {Plant Cell},
number = 10,
volume = 30,
place = {United States},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1105/tpc.18.00521

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Cited by: 13 works
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