skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Comparative studies of glycolytic pathways and channeling under in vitro and in vivo modes

 [1];  [2];  [1];  [3];  [3]; ORCiD logo [2]; ORCiD logo [1]
  1. Dept. of Energy, Environmental and Chemical Engineering, Washington University, St. Louis MO 63130
  2. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071 China
  3. Lawrence Berkeley National Laboratory, Emeryville CA 64608
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
Grant/Contract Number:  
DESC0018324; AC02-05CH11231
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
AIChE Journal
Additional Journal Information:
Journal Name: AIChE Journal Journal Volume: 65 Journal Issue: 2; Journal ID: ISSN 0001-1541
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United States

Citation Formats

Abernathy, Mary H., Zhang, Yuchen, Hollinshead, Whitney D., Wang, George, Baidoo, Edward E. K., Liu, Tiangang, and Tang, Yinjie J. Comparative studies of glycolytic pathways and channeling under in vitro and in vivo modes. United States: N. p., 2018. Web. doi:10.1002/aic.16367.
Abernathy, Mary H., Zhang, Yuchen, Hollinshead, Whitney D., Wang, George, Baidoo, Edward E. K., Liu, Tiangang, & Tang, Yinjie J. Comparative studies of glycolytic pathways and channeling under in vitro and in vivo modes. United States. doi:10.1002/aic.16367.
Abernathy, Mary H., Zhang, Yuchen, Hollinshead, Whitney D., Wang, George, Baidoo, Edward E. K., Liu, Tiangang, and Tang, Yinjie J. Fri . "Comparative studies of glycolytic pathways and channeling under in vitro and in vivo modes". United States. doi:10.1002/aic.16367.
title = {Comparative studies of glycolytic pathways and channeling under in vitro and in vivo modes},
author = {Abernathy, Mary H. and Zhang, Yuchen and Hollinshead, Whitney D. and Wang, George and Baidoo, Edward E. K. and Liu, Tiangang and Tang, Yinjie J.},
abstractNote = {},
doi = {10.1002/aic.16367},
journal = {AIChE Journal},
number = 2,
volume = 65,
place = {United States},
year = {2018},
month = {9}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/aic.16367

Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

High-throughput metabolic flux analysis based on gas chromatography–mass spectrometry derived 13C constraints
journal, February 2004

Direct Evidence for Metabolon Formation and Substrate Channeling in Recombinant TCA Cycle Enzymes
journal, August 2016

Channeling in native microbial pathways: Implications and challenges for metabolic engineering
journal, November 2017

Effect of a pyruvate kinase ( pykF -gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli
journal, June 2004

Metabolic engineering and adaptive evolution of Escherichia coli KO11 for ethanol production through the Entner-Doudoroff and the pentose phosphate pathways : Engineering of E-D and PP pathways in KO11 pgi- knockout
journal, November 2016

  • Huerta-Beristain, Gerardo; Cabrera-Ruiz, Rosina; Hernandez-Chavez, Georgina
  • Journal of Chemical Technology & Biotechnology, Vol. 92, Issue 5
  • DOI: 10.1002/jctb.5138

Combination of Entner-Doudoroff Pathway with MEP Increases Isoprene Production in Engineered Escherichia coli
journal, December 2013

Glycolytic Enzymes Associate Dynamically with Mitochondria in Response to Respiratory Demand and Support Substrate Channeling
journal, November 2007

  • Graham, James W. A.; Williams, Thomas C. R.; Morgan, Megan
  • The Plant Cell, Vol. 19, Issue 11
  • DOI: 10.1105/tpc.107.053371

Possible Pitfalls of Flux Calculations Based on 13C-Labeling
journal, April 2001

  • van Winden, Wouter; Verheijen, Peter; Heijnen, Sef
  • Metabolic Engineering, Vol. 3, Issue 2
  • DOI: 10.1006/mben.2000.0174

eQuilibrator--the biochemical thermodynamics calculator
journal, November 2011

  • Flamholz, A.; Noor, E.; Bar-Even, A.
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr874

Modulation of the Major Paths of Carbon in Photorespiratory Mutants of Synechocystis
journal, January 2011

The role of dynamic enzyme assemblies and substrate channelling in metabolic regulation
journal, May 2018

Large-scale in vivo flux analysis shows rigidity and suboptimal performance of Bacillus subtilis metabolism
journal, May 2005

  • Fischer, Eliane; Sauer, Uwe
  • Nature Genetics, Vol. 37, Issue 6
  • DOI: 10.1038/ng1555

In Vitro Reconstitution and Optimization of the Entire Pathway to Convert Glucose into Fatty Acid
journal, January 2017

Interaction between phosphofructokinase and aldolase from Saccharomyces cerevisiae studied by aqueous two-phase partitioning
journal, February 2001

  • Matic, Sandra; Widell, Susanne; Åkerlund, Hans-Erik
  • Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 751, Issue 2
  • DOI: 10.1016/S0378-4347(00)00492-8

Characterization of physiological responses to 22 gene knockouts in Escherichia coli central carbon metabolism
journal, September 2016

Substrate-driven chemotactic assembly in an enzyme cascade
journal, December 2017

  • Zhao, Xi; Palacci, Henri; Yadav, Vinita
  • Nature Chemistry, Vol. 10, Issue 3
  • DOI: 10.1038/nchem.2905

Global metabolic regulation analysis for Escherichia coli K12 based on protein expression by 2-dimensional electrophoresis and enzyme activity measurement
journal, January 2003

A genome-scale Escherichia coli kinetic metabolic model k-ecoli457 satisfying flux data for multiple mutant strains
journal, December 2016

  • Khodayari, Ali; Maranas, Costas D.
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms13806

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins
journal, November 2017

  • Harmon, Tyler S.; Holehouse, Alex S.; Rosen, Michael K.
  • eLife, Vol. 6
  • DOI: 10.7554/eLife.30294

Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene
journal, November 2010

Glycolytic strategy as a tradeoff between energy yield and protein cost
journal, April 2013

  • Flamholz, A.; Noor, E.; Bar-Even, A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 24
  • DOI: 10.1073/pnas.1215283110

Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis
journal, November 2011

  • Young, Jamey D.; Shastri, Avantika A.; Stephanopoulos, Gregory
  • Metabolic Engineering, Vol. 13, Issue 6
  • DOI: 10.1016/j.ymben.2011.08.002

Krebs Cycle Metabolon: Structural Evidence of Substrate Channeling Revealed by Cross-Linking and Mass Spectrometry
journal, December 2014

  • Wu, Fei; Minteer, Shelley
  • Angewandte Chemie International Edition, Vol. 54, Issue 6
  • DOI: 10.1002/anie.201409336

Allosteric and non-allosteric E. coli phosphofructokinases: effects on growth
journal, April 1978

  • Robinson, John P.; Fraenkel, D. G.
  • Biochemical and Biophysical Research Communications, Vol. 81, Issue 3
  • DOI: 10.1016/0006-291X(78)91430-4

Improved Production of l-Threonine in Escherichia coli by Use of a DNA Scaffold System
journal, November 2012

  • Lee, Jun Hyoung; Jung, Suk-Chae; Bui, Le Minh
  • Applied and Environmental Microbiology, Vol. 79, Issue 3
  • DOI: 10.1128/AEM.02578-12

A Map of Protein-Metabolite Interactions Reveals Principles of Chemical Communication
journal, January 2018

Isotopically nonstationary 13 C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation
journal, November 2014

  • Ma, Fangfang; Jazmin, Lara J.; Young, Jamey D.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 47
  • DOI: 10.1073/pnas.1319485111

Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells
journal, July 2010

Global Rebalancing of Cellular Resources by Pleiotropic Point Mutations Illustrates a Multi-scale Mechanism of Adaptive Evolution
journal, April 2016

Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants
journal, October 2016

  • Hollinshead, Whitney D.; Rodriguez, Sarah; Martin, Hector Garcia
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0630-y

WUFlux: an open-source platform for 13C metabolic flux analysis of bacterial metabolism
journal, November 2016

Substrate channeling and enzyme complexes for biotechnological applications
journal, November 2011

Synthetic protein scaffolds provide modular control over metabolic flux
journal, August 2009

  • Dueber, John E.; Wu, Gabriel C.; Malmirchegini, G. Reza
  • Nature Biotechnology, Vol. 27, Issue 8, p. 753-759
  • DOI: 10.1038/nbt.1557

Genome-Scale Architecture of Small Molecule Regulatory Networks and the Fundamental Trade-Off between Regulation and Enzymatic Activity
journal, September 2017

Biomolecular condensates: organizers of cellular biochemistry
journal, February 2017

  • Banani, Salman F.; Lee, Hyun O.; Hyman, Anthony A.
  • Nature Reviews Molecular Cell Biology, Vol. 18, Issue 5
  • DOI: 10.1038/nrm.2017.7

Pathway engineering for the production of aromatic compounds in Escherichia coli
journal, May 1996

  • Floras, Noemí; Xiao, Jimmy; Berry, Alan
  • Nature Biotechnology, Vol. 14, Issue 5
  • DOI: 10.1038/nbt0596-620

New tools for mass isotopomer data evaluation in13C flux analysis: Mass isotope correction, data consistency checking, and precursor relationships
journal, January 2004

  • Wahl, S. Aljoscha; Dauner, Michael; Wiechert, Wolfgang
  • Biotechnology and Bioengineering, Vol. 85, Issue 3
  • DOI: 10.1002/bit.10909

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
journal, June 2009

  • Bennett, Bryson D.; Kimball, Elizabeth H.; Gao, Melissa
  • Nature Chemical Biology, Vol. 5, Issue 8
  • DOI: 10.1038/nchembio.186

Substrate channelling as an approach to cascade reactions
journal, March 2016

  • Wheeldon, Ian; Minteer, Shelley D.; Banta, Scott
  • Nature Chemistry, Vol. 8, Issue 4
  • DOI: 10.1038/nchem.2459