Response of Aquatic Bacterial Communities to Hydraulic Fracturing in Northwestern Pennsylvania: A Five-Year Study

Ulrich, Nikea; Kirchner, Veronica; Drucker, Rebecca; Wright, Justin R.; McLimans, Christopher J.; Hazen, Terry C.; Campa, Maria F.; Grant, Christopher J.; Lamendella, Regina

doi:10.1038/s41598-018-23679-7

Title: Response of Aquatic Bacterial Communities to Hydraulic Fracturing in Northwestern Pennsylvania: A Five-Year Study

Journal Article · 08 April 2018 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-018-23679-7 · OSTI ID:1471862

Ulrich, Nikea ^[1]; Kirchner, Veronica ^[1];

^[1]; Wright, Justin R. ^[2]; McLimans, Christopher J. ^[1];

^[3]; Campa, Maria F. ^[3]; Grant, Christopher J. ^[1]; Lamendella, Regina ^[1]

Juniata College, Hungtingdon, PA (United States). Department of Biology
Wright Labs LLC, Huntingdon, PA (United States)
Univ. of Tennessee, Knoxville, TN (United States). Department of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division

Horizontal drilling and hydraulic fracturing extraction procedures have become increasingly present in Pennsylvania where the Marcellus Shale play is largely located. The potential for long-term environmental impacts to nearby headwater stream ecosystems and aquatic bacterial assemblages is still incompletely understood. Here, we perform high-throughput sequencing of the 16 S rRNA gene to characterize the bacterial community structure of water, sediment, and other environmental samples (n = 189) from 31 headwater stream sites exhibiting different histories of fracking activity in northwestern Pennsylvania over five years (2012–2016). Stream pH was identified as a main driver of bacterial changes within the streams and fracking activity acted as an environmental selector for certain members at lower taxonomic levels within stream sediment. Methanotrophic and methanogenic bacteria (i.e. Methylocystaceae, Beijerinckiaceae, and Methanobacterium) were significantly enriched in sites exhibiting Marcellus shale activity (MSA+) compared to MSA- streams. This study highlighted potential sentinel taxa associated with nascent Marcellus shale activity and some of these taxa remained as stable biomarkers across this five-year study. Identifying the presence and functionality of specific microbial consortia within fracking-impacted streams will provide a clearer understanding of the natural microbial community’s response to fracking and inform in situ remediation strategies.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231; AC05-00OR22725

OSTI ID:: 1471862

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 8; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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The role of natural gas as a primary fuel in the near future, including comparisons of acquisition, transmission and waste handling costs of as with competitive alternatives Liang, Fang-Yu; Ryvak, Marta; Sayeed, Sara Chemistry Central Journal, Vol. 6, Issue S1 https://doi.org/10.1186/1752-153x-6-s1-s4	journal	April 2012
EMPeror: a tool for visualizing high-throughput microbial community data Vázquez-Baeza, Yoshiki; Pirrung, Meg; Gonzalez, Antonio GigaScience, Vol. 2, Issue 1 https://doi.org/10.1186/2047-217X-2-16	journal	November 2013
Metagenomic biomarker discovery and explanation Segata, Nicola; Izard, Jacques; Waldron, Levi Genome Biology, Vol. 12, Issue 6 https://doi.org/10.1186/gb-2011-12-6-r60	journal	January 2011
Do biofilm communities respond to the chemical signatures of fracking? A test involving streams in North-central Arkansas Johnson, Wilson H.; Douglas, Marlis R.; Lewis, Jeffrey A. BMC Microbiology, Vol. 17, Issue 1 https://doi.org/10.1186/s12866-017-0926-5	journal	February 2017
Succession of endophytic fungi and arbuscular mycorrhizal fungi associated with the growth of plant and their correlation with secondary metabolites in the roots of plants Dang, Hanli; Zhang, Tao; Wang, Zhongke BMC Plant Biology, Vol. 21, Issue 1 https://doi.org/10.1186/s12870-021-02942-6	journal	April 2021
CoNet app: inference of biological association networks using Cytoscape Faust, Karoline; Raes, Jeroen F1000Research, Vol. 5 https://doi.org/10.12688/f1000research.9050.1	journal	January 2016
CoNet app: inference of biological association networks using Cytoscape Faust, Karoline; Raes, Jeroen F1000Research, Vol. 5 https://doi.org/10.12688/f1000research.9050.2	journal	January 2016
phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data McMurdie, Paul J.; Holmes, Susan PLoS ONE, Vol. 8, Issue 4 https://doi.org/10.1371/journal.pone.0061217	journal	April 2013
Stream Vulnerability to Widespread and Emergent Stressors: A Focus on Unconventional Oil and Gas Entrekin, Sally A.; Maloney, Kelly O.; Kapo, Katherine E. PLOS ONE, Vol. 10, Issue 9 https://doi.org/10.1371/journal.pone.0137416	journal	September 2015
Rapid expansion of natural gas development poses a threat to surface waters Entrekin, Sally; Evans-White, Michelle; Johnson, Brent Frontiers in Ecology and the Environment, Vol. 9, Issue 9 https://doi.org/10.1890/110053	journal	October 2011
The ecology of methane in streams and rivers: patterns, controls, and global significance Stanley, Emily H.; Casson, Nora J.; Christel, Samuel T. Ecological Monographs, Vol. 86, Issue 2 https://doi.org/10.1890/15-1027	journal	May 2016
Hydraulic Fracturing and Organic Compounds - Uses, Disposal and Challenges Carter, Kimberly E.; Hammack, Richard W.; Hakala, J. Alexandra SPE Eastern Regional Meeting https://doi.org/10.2118/165692-MS	conference	August 2013
Hydraulic Fracturing and Organic Compounds - Uses, Disposal and Challenges Carter, Kimberly E.; Hammack, Richard W.; Hakala, J. Alexandra SPE Eastern Regional Meeting https://doi.org/10.2118/165692-ms	conference	August 2013
Wastewater Management and Marcellus Shale Gas Development: Trends, Drivers, and Planning Implications Rahm, Brian G.; Bates, Josephine; Bertoia, Lara R. SSRN Electronic Journal https://doi.org/10.2139/ssrn.2176530	journal	January 2012
Rhodovastum atsumiense gen. nov., sp. nov., a phototrophic alphaproteobacterium isolated from paddy soil Okamura, Keiko; Hisada, Takayoshi; Kanbe, Toshio The Journal of General and Applied Microbiology, Vol. 55, Issue 1 https://doi.org/10.2323/jgam.55.43	journal	January 2009
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Assessing impacts of unconventional natural gas extraction on microbial communities in headwater stream ecosystems in Northwestern Pennsylvania Trexler, Ryan; Solomon, Caroline; Brislawn, Colin J. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00522	journal	November 2014
Landscape Disturbance from Unconventional and Conventional Oil and Gas Development in the Marcellus Shale Region of Pennsylvania, USA Slonecker, E.; Milheim, Lesley Environments, Vol. 2, Issue 4 https://doi.org/10.3390/environments2020200	journal	June 2015
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Estimation of regional air-quality damages from Marcellus Shale natural gas extraction in Pennsylvania Litovitz, Aviva; Curtright, Aimee; Abramzon, Shmuel Institute of Physics (IOP) https://doi.org/10.5446/39480	audiovisual	January 2013

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Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction Campa, Maria Fernanda; Techtmann, Stephen M.; Ladd, Mallory P. Applied and Environmental Microbiology, Vol. 85, Issue 21 https://doi.org/10.1128/aem.01336-19	journal	August 2019
Unconventional Oil and Gas Energy Systems: An Unidentified Hotspot of Antimicrobial Resistance? Campa, Maria Fernanda; Wolfe, Amy K.; Techtmann, Stephen M. Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02392	journal	October 2019
Shale gas development has limited effects on stream biology and geochemistry in a gradient-based, multiparameter study in Pennsylvania Mumford, Adam C.; Maloney, Kelly O.; Akob, Denise M. Proceedings of the National Academy of Sciences, Vol. 117, Issue 7 https://doi.org/10.1073/pnas.1911458117	journal	February 2020
Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction Campa, Maria Fernanda; Techtmann, Stephen M.; Ladd, Mallory P. Applied and Environmental Microbiology, Vol. 85, Issue 21 https://doi.org/10.1128/aem.01336-19	journal	August 2019

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