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Title: Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili

Abstract

Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR-1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well-characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose-binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose-coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals.

Authors:
ORCiD logo [1];  [2];  [3];  [1];  [1];  [4];  [1]
  1. VTT Technical Research Centre of Finland Ltd, Espoo Finland
  2. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing MI USA, The Molecular Foundry, Lawrence Berkeley National Laboratory, Molecular Biophysics and Integrated Bioimaging Division, Synthetic Biology Institute, Berkeley CA USA
  3. VTT Technical Research Centre of Finland Ltd, Espoo Finland, Current affiliation: Solar Foods Ltd, Helsinki Finland
  4. The Molecular Foundry, Lawrence Berkeley National Laboratory, Molecular Biophysics and Integrated Bioimaging Division, Synthetic Biology Institute, Berkeley CA USA
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); Office of Naval Research (ONR) (United States); The Finnish Funding Agency for Technology and Innovation (Tekes) (Finland); Academy of Finland
OSTI Identifier:
1471141
Alternate Identifier(s):
OSTI ID: 1472156; OSTI ID: 1483273
Grant/Contract Number:  
287011; AC02-05CH11231; N000141310551; 2299/31/2012; 40044/13; 277121; 272569
Resource Type:
Published Article
Journal Name:
Microbial Biotechnology (Online)
Additional Journal Information:
Journal Name: Microbial Biotechnology (Online) Journal Volume: 11 Journal Issue: 6; Journal ID: ISSN 1751-7915
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lienemann, Michael, TerAvest, Michaela A., Pitkänen, Juha-Pekka, Stuns, Ingmar, Penttilä, Merja, Ajo-Franklin, Caroline M., and Jäntti, Jussi. Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili. United Kingdom: N. p., 2018. Web. doi:10.1111/1751-7915.13309.
Lienemann, Michael, TerAvest, Michaela A., Pitkänen, Juha-Pekka, Stuns, Ingmar, Penttilä, Merja, Ajo-Franklin, Caroline M., & Jäntti, Jussi. Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili. United Kingdom. doi:10.1111/1751-7915.13309.
Lienemann, Michael, TerAvest, Michaela A., Pitkänen, Juha-Pekka, Stuns, Ingmar, Penttilä, Merja, Ajo-Franklin, Caroline M., and Jäntti, Jussi. Mon . "Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili". United Kingdom. doi:10.1111/1751-7915.13309.
@article{osti_1471141,
title = {Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili},
author = {Lienemann, Michael and TerAvest, Michaela A. and Pitkänen, Juha-Pekka and Stuns, Ingmar and Penttilä, Merja and Ajo-Franklin, Caroline M. and Jäntti, Jussi},
abstractNote = {Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR-1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well-characterized genetic tools. However, means to specifically immobilize this organism at solid substrates as electroactive biofilms have not been tested previously. Here, we show that mannose-binding Fim pili can be produced in exoelectrogenic E. coli and can be used to selectively attach cells to a mannose-coated material. Importantly, cells expressing fim genes retained current production by the heterologous Mtr electron conduit. Our results demonstrate the versatility of the exoelectrogenic E. coli system and motivate future work that aims to produce patterned biofilms for bioelectronic devices that can respond to various biochemical signals.},
doi = {10.1111/1751-7915.13309},
journal = {Microbial Biotechnology (Online)},
number = 6,
volume = 11,
place = {United Kingdom},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/1751-7915.13309

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Cited by: 1 work
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