Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser

Abstract

Ultrafast isomerization of retinal is the primary step in photoresponsive biological functions including vision in humans and ion transport across bacterial membranes. In this study, we used an x-ray laser to study the subpicosecond structural dynamics of retinal isomerization in the light-driven proton pump bacteriorhodopsin. A series of structural snapshots with near-atomic spatial resolution and temporal resolution in the femtosecond regime show how the excited all-trans retinal samples conformational states within the protein binding pocket before passing through a twisted geometry and emerging in the 13-cis conformation. In conclusion, our findings suggest ultrafast collective motions of aspartic acid residues and functional water molecules in the proximity of the retinal Schiff base as a key facet of this stereoselective and efficient photochemical reaction.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [3];  [4]; ORCiD logo [1];  [5]; ORCiD logo [6];  [1];  [1]; ORCiD logo [7];  [8];  [8]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [3];  [1];  [1];  [9];  [1] more »; ORCiD logo [5]; ORCiD logo [10];  [10]; ORCiD logo [11];  [1]; ORCiD logo [9]; ORCiD logo [9];  [3]; ORCiD logo [6]; ORCiD logo [12]; ORCiD logo [8]; ORCiD logo [1] « less
+ Show Author Affiliations
  1. Paul Scherrer Institut (Switzerland). Division of Biology and Chemistry–Laboratory for Biomolecular Research
  2. Paul Scherrer Institut (Switzerland). Division of Biology and Chemistry–Laboratory for Biomolecular Research and Photon Science Division–Swiss Light Source
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  4. Paul Scherrer Institut (Switzerland). Science IT
  5. Paul Scherrer Institut (Switzerland). SwissFEL
  6. The Hebrew University of Jerusalem (Israel). Fritz Haber Center for Molecular Dynamics, Institute of Chemistry
  7. Paul Scherrer Institut (Switzerland). SwissFEL and Photon Science Division–Swiss Light Source
  8. University of Gothenburg (Sweden). Department of Chemistry and Molecular Biology
  9. RIKEN SPring-8 Center (Japan); Kyoto University (Japan). Department of Cell Biology, Graduate School of Medicine
  10. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany).Center for Free-Electron Laser Science (CFEL)
  11. Arizona State Univ., Tempe, AZ (United States). Department of Physics
  12. Paul Scherrer Institut (Switzerland). Division of Biology and Chemistry–Laboratory for Biomolecular Research ; ETH Zürich (Switzerland). Department of Biology
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1470908
Grant/Contract Number:  
AC02-76SF00515; 637295; 678169; FP7/2007-2013; FP7-PEOPLE-2011-ITN 317079; 310030_153145; PZ00P3_174169; KAW 2014.0275; SRL10-0036; 2015–00560; 349-2011-6485; 31003A_159558; 142-004
Resource Type:
Accepted Manuscript
Journal Name:
Science
Additional Journal Information:
Journal Volume: 361; Journal Issue: 6398; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Nogly, Przemyslaw, Weinert, Tobias, James, Daniel, Carbajo, Sergio, Ozerov, Dmitry, Furrer, Antonia, Gashi, Dardan, Borin, Veniamin, Skopintsev, Petr, Jaeger, Kathrin, Nass, Karol, Båth, Petra, Bosman, Robert, Koglin, Jason, Seaberg, Matthew, Lane, Thomas, Kekilli, Demet, Brünle, Steffen, Tanaka, Tomoyuki, Wu, Wenting, Milne, Christopher, White, Thomas, Barty, Anton, Weierstall, Uwe, Panneels, Valerie, Nango, Eriko, Iwata, So, Hunter, Mark, Schapiro, Igor, Schertler, Gebhard, Neutze, Richard, and Standfuss, Jörg. Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser. United States: N. p., 2018. Web. doi:10.1126/science.aat0094.
Copy to clipboard
Nogly, Przemyslaw, Weinert, Tobias, James, Daniel, Carbajo, Sergio, Ozerov, Dmitry, Furrer, Antonia, Gashi, Dardan, Borin, Veniamin, Skopintsev, Petr, Jaeger, Kathrin, Nass, Karol, Båth, Petra, Bosman, Robert, Koglin, Jason, Seaberg, Matthew, Lane, Thomas, Kekilli, Demet, Brünle, Steffen, Tanaka, Tomoyuki, Wu, Wenting, Milne, Christopher, White, Thomas, Barty, Anton, Weierstall, Uwe, Panneels, Valerie, Nango, Eriko, Iwata, So, Hunter, Mark, Schapiro, Igor, Schertler, Gebhard, Neutze, Richard, & Standfuss, Jörg. Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser. United States. https://doi.org/10.1126/science.aat0094
Copy to clipboard
Nogly, Przemyslaw, Weinert, Tobias, James, Daniel, Carbajo, Sergio, Ozerov, Dmitry, Furrer, Antonia, Gashi, Dardan, Borin, Veniamin, Skopintsev, Petr, Jaeger, Kathrin, Nass, Karol, Båth, Petra, Bosman, Robert, Koglin, Jason, Seaberg, Matthew, Lane, Thomas, Kekilli, Demet, Brünle, Steffen, Tanaka, Tomoyuki, Wu, Wenting, Milne, Christopher, White, Thomas, Barty, Anton, Weierstall, Uwe, Panneels, Valerie, Nango, Eriko, Iwata, So, Hunter, Mark, Schapiro, Igor, Schertler, Gebhard, Neutze, Richard, and Standfuss, Jörg. Fri . "Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser". United States. https://doi.org/10.1126/science.aat0094. https://www.osti.gov/servlets/purl/1470908.
Copy to clipboard
@article{osti_1470908,
title = {Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser},
author = {Nogly, Przemyslaw and Weinert, Tobias and James, Daniel and Carbajo, Sergio and Ozerov, Dmitry and Furrer, Antonia and Gashi, Dardan and Borin, Veniamin and Skopintsev, Petr and Jaeger, Kathrin and Nass, Karol and Båth, Petra and Bosman, Robert and Koglin, Jason and Seaberg, Matthew and Lane, Thomas and Kekilli, Demet and Brünle, Steffen and Tanaka, Tomoyuki and Wu, Wenting and Milne, Christopher and White, Thomas and Barty, Anton and Weierstall, Uwe and Panneels, Valerie and Nango, Eriko and Iwata, So and Hunter, Mark and Schapiro, Igor and Schertler, Gebhard and Neutze, Richard and Standfuss, Jörg},
abstractNote = {Ultrafast isomerization of retinal is the primary step in photoresponsive biological functions including vision in humans and ion transport across bacterial membranes. In this study, we used an x-ray laser to study the subpicosecond structural dynamics of retinal isomerization in the light-driven proton pump bacteriorhodopsin. A series of structural snapshots with near-atomic spatial resolution and temporal resolution in the femtosecond regime show how the excited all-trans retinal samples conformational states within the protein binding pocket before passing through a twisted geometry and emerging in the 13-cis conformation. In conclusion, our findings suggest ultrafast collective motions of aspartic acid residues and functional water molecules in the proximity of the retinal Schiff base as a key facet of this stereoselective and efficient photochemical reaction.},
doi = {10.1126/science.aat0094},
journal = {Science},
number = 6398,
volume = 361,
place = {United States},
year = {2018},
month = {7}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1126/science.aat0094
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 40 works
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Bacteriorhodopsin: Would the real structural intermediates please stand up?
journal, March 2015

  • Wickstrand, Cecilia; Dods, Robert; Royant, Antoine
  • Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1850, Issue 3
  • DOI: 10.1016/j.bbagen.2014.05.021

Cheetah : software for high-throughput reduction and analysis of serial femtosecond X-ray diffraction data
journal, May 2014

  • Barty, Anton; Kirian, Richard A.; Maia, Filipe R. N. C.
  • Journal of Applied Crystallography, Vol. 47, Issue 3
  • DOI: 10.1107/S1600576714007626

CrystFEL : a software suite for snapshot serial crystallography
journal, March 2012

  • White, Thomas A.; Kirian, Richard A.; Martin, Andrew V.
  • Journal of Applied Crystallography, Vol. 45, Issue 2
  • DOI: 10.1107/S0021889812002312

The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS)
journal, March 2010

Time-resolved pump-probe experiments at the LCLS
journal, January 2010

  • Glownia, James M.; Cryan, J.; Andreasson, J.
  • Optics Express, Vol. 18, Issue 17
  • DOI: 10.1364/OE.18.017620

Time-resolved structural studies at synchrotrons and X-ray free electron lasers: opportunities and challenges
journal, October 2012

Molecular Dynamics Simulation of the Excited-State Dynamics of Bacteriorhodopsin
journal, December 1997

Insights into excited-state and isomerization dynamics of bacteriorhodopsin from ultrafast transient UV absorption
journal, March 2006

  • Schenkl, S.; van Mourik, F.; Friedman, N.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 11
  • DOI: 10.1073/pnas.0506303103

PHENIX : building new software for automated crystallographic structure determination
journal, October 2002

  • Adams, Paul D.; Grosse-Kunstleve, Ralf W.; Hung, Li-Wei
  • Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 11
  • DOI: 10.1107/S0907444902016657

Breaking the indexing ambiguity in serial crystallography
journal, December 2013

  • Brehm, Wolfgang; Diederichs, Kay
  • Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 1
  • DOI: 10.1107/S1399004713025431

The Coherent X-ray Imaging instrument at the Linac Coherent Light Source
journal, April 2015

  • Liang, Mengning; Williams, Garth J.; Messerschmidt, Marc
  • Journal of Synchrotron Radiation, Vol. 22, Issue 3
  • DOI: 10.1107/S160057751500449X

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
text, January 2016

Crystallographic Structure of the K Intermediate of Bacteriorhodopsin: Conservation of Free Energy after Photoisomerization of the Retinal
journal, August 2002

UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004

  • Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
  • Journal of Computational Chemistry, Vol. 25, Issue 13
  • DOI: 10.1002/jcc.20084

Ultrafast anisotropic protein quake propagation after CO photodissociation in myoglobin
journal, September 2016

  • Brinkmann, Levin U. L.; Hub, Jochen S.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 38
  • DOI: 10.1073/pnas.1603539113

Excited-state dynamics of bacteriorhodopsin probed by broadband femtosecond fluorescence spectroscopy
journal, January 2005

  • Schmidt, B.; Sobotta, C.; Heinz, B.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1706, Issue 1-2
  • DOI: 10.1016/j.bbabio.2004.10.008

Coot model-building tools for molecular graphics
journal, November 2004

  • Emsley, Paul; Cowtan, Kevin
  • Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
  • DOI: 10.1107/S0907444904019158

Primary photochemical event in bacteriorhodopsin: study with artificial pigments
journal, February 1985

  • Sheves, Mordechai; Friedman, Noga; Albeck, Amnon
  • Biochemistry, Vol. 24, Issue 5
  • DOI: 10.1021/bi00326a031

Vibrational Spectrum of the J-625 Intermediate in the Room Temperature Bacteriorhodopsin Photocycle
journal, May 2000

  • Atkinson, G. H.; Ujj, L.; Zhou, Yidong
  • The Journal of Physical Chemistry A, Vol. 104, Issue 18
  • DOI: 10.1021/jp9918306

Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
journal, August 2014

  • Arnlund, David; Johansson, Linda C.; Wickstrand, Cecilia
  • Nature Methods, Vol. 11, Issue 9
  • DOI: 10.1038/nmeth.3067

Computational evidence in favor of a two-state, two-mode model of the retinal chromophore photoisomerization
journal, August 2000

  • Gonzalez-Luque, R.; Garavelli, M.; Bernardi, F.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 17
  • DOI: 10.1073/pnas.97.17.9379

Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein
journal, May 2016

Picosecond time-resolved resonance Raman spectroscopy of the initial trans to cis isomerization in the bacteriorhodopsin photocycle
journal, March 1989

Spectral encoding of x-ray/optical relative delay
journal, January 2011

  • Bionta, Mina R.; Lemke, H. T.; Cryan, J. P.
  • Optics Express, Vol. 19, Issue 22
  • DOI: 10.1364/OE.19.021855

Protein states and proteinquakes.
journal, August 1985

  • Ansari, A.; Berendzen, J.; Bowne, S. F.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 15
  • DOI: 10.1073/pnas.82.15.5000

Femtosecond Infrared Spectroscopy of Bacteriorhodopsin Chromophore Isomerization
journal, August 2002

Achieving few-femtosecond time-sorting at hard X-ray free-electron lasers
journal, February 2013

Coherent Control of Retinal Isomerization in Bacteriorhodopsin
journal, September 2006

Optical laser systems at the Linac Coherent Light Source
journal, April 2015

  • Minitti, Michael P.; Robinson, Joseph S.; Coffee, Ryan N.
  • Journal of Synchrotron Radiation, Vol. 22, Issue 3
  • DOI: 10.1107/S1600577515006244

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
journal, August 2016

  • Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms12314

Comparative investigation of the photoisomerization of the protonated and unprotonated n-butylamine Schiff bases of 9-cis-, 11-cis-, 13-cis-, and all-trans-retinals
journal, March 1986

  • Freedman, Kenn A.; Becker, Ralph S.
  • Journal of the American Chemical Society, Vol. 108, Issue 6
  • DOI: 10.1021/ja00266a020

Linac Coherent Light Source data analysis using psana
journal, March 2016

  • Damiani, D.; Dubrovin, M.; Gaponenko, I.
  • Journal of Applied Crystallography, Vol. 49, Issue 2
  • DOI: 10.1107/S1600576716004349

Microbial and Animal Rhodopsins: Structures, Functions, and Molecular Mechanisms
journal, September 2013

  • Ernst, Oliver P.; Lodowski, David T.; Elstner, Marcus
  • Chemical Reviews, Vol. 114, Issue 1
  • DOI: 10.1021/cr4003769

Bicycle-pedal model for the first step in the vision process
journal, April 1976

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
journal, December 2014

Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography
journal, September 2017

  • Coquelle, Nicolas; Sliwa, Michel; Woodhouse, Joyce
  • Nature Chemistry, Vol. 10, Issue 1
  • DOI: 10.1038/nchem.2853

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
journal, September 2017

Direct observation of the femtosecond excited-state cis-trans isomerization in bacteriorhodopsin
journal, May 1988

Lipidic cubic phases: A novel concept for the crystallization of membrane proteins
journal, December 1996

  • Landau, E. M.; Rosenbusch, J. P.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 25
  • DOI: 10.1073/pnas.93.25.14532

Aborted double bicycle-pedal isomerization with hydrogen bond breaking is the primary event of bacteriorhodopsin proton pumping
journal, November 2010

  • Altoe, P.; Cembran, A.; Olivucci, M.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 47
  • DOI: 10.1073/pnas.1007000107

PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
journal, December 2019

Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons
text, January 2017

First lasing and operation of an ångstrom-wavelength free-electron laser
journal, August 2010

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
journal, February 2014

  • Weierstall, Uwe; James, Daniel; Wang, Chong
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms4309

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Ultrafast myoglobin structural dynamics observed with an X-ray free-electron laser
journal, April 2015

  • Levantino, Matteo; Schirò, Giorgio; Lemke, Henrik Till
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms7772

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
journal, March 2015

Femtosecond spectroscopy of the photoisomerisation of the protonated Schiff base of all-trans retinal
journal, December 1996

Nature of the Surface Crossing Process in Bacteriorhodopsin:  Computer Simulations of the Quantum Dynamics of the Primary Photochemical Event
journal, October 2001

  • Warshel, A.; Chu, Z. T.
  • The Journal of Physical Chemistry B, Vol. 105, Issue 40
  • DOI: 10.1021/jp010704a

Optogenetic Tools for Subcellular Applications in Neuroscience
journal, November 2017

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
text, January 2016

  • Nogly, Przemyslaw; Panneels, Valerie; Nelson, Garrett
  • Deutsches Elektronen-Synchrotron, DESY, Hamburg
  • DOI: 10.3204/pubdb-2016-03927

Specific Damage Induced by X-ray Radiation and Structural Changes in the Primary Photoreaction of Bacteriorhodopsin
journal, November 2002

A three-dimensional movie of structural changes in bacteriorhodopsin
journal, December 2016

  • Nango, Eriko; Royant, Antoine; Kubo, Minoru
  • Science, Vol. 354, Issue 6319, p. 1552-1557
  • DOI: 10.1126/science.aah3497

High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle
journal, October 1999

  • Edman, Karl; Nollert, Peter; Royant, Antoine
  • Nature, Vol. 401, Issue 6755
  • DOI: 10.1038/44623

Femtosecond Time-Resolved Stimulated Raman Reveals the Birth of Bacteriorhodopsin’s J and K Intermediates
journal, June 2009

  • Shim, Sangdeok; Dasgupta, Jyotishman; Mathies, Richard A.
  • Journal of the American Chemical Society, Vol. 131, Issue 22
  • DOI: 10.1021/ja809137x

Control of retinal isomerization in bacteriorhodopsin in the high-intensity regime
journal, June 2009

  • Florean, A. C.; Cardoza, D.; White, J. L.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 27
  • DOI: 10.1073/pnas.0904589106

Real-time spectroscopy of transition states in bacteriorhodopsin during retinal isomerization
journal, November 2001

  • Kobayashi, Takayoshi; Saito, Takashi; Ohtani, Hiroyuki
  • Nature, Vol. 414, Issue 6863
  • DOI: 10.1038/35107042

Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation
journal, September 2015

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
text, January 2015

    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments
    journal, October 2019

    • Andersson, Rebecka; Safari, Cecilia; Båth, Petra
    • Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10
    • DOI: 10.1107/s2059798319012695

    Molecular mechanism for thermal denaturation of thermophilic rhodopsin
    journal, January 2019

    • Misra, Ramprasad; Hirshfeld, Amiram; Sheves, Mordechai
    • Chemical Science, Vol. 10, Issue 31
    • DOI: 10.1039/c9sc00855a

    Coherent phenomena in photosynthetic light harvesting: part one—theory and spectroscopy
    journal, September 2018

    • Rathbone, Harry W.; Davis, Jeffery A.; Michie, Katharine A.
    • Biophysical Reviews, Vol. 10, Issue 5
    • DOI: 10.1007/s12551-018-0451-2

    Collective exchange processes reveal an active site proton cage in bacteriorhodopsin
    journal, January 2020

    • Friedrich, Daniel; Brünig, Florian N.; Nieuwkoop, Andrew J.
    • Communications Biology, Vol. 3, Issue 1
    • DOI: 10.1038/s42003-019-0733-7

    Biological single-particle imaging using XFELs – towards the next resolution revolution
    text, January 2018

    Time-resolved serial femtosecond crystallography at the European XFEL
    text, January 2019

    • Pandey, Suraj; Bean, Richard; Sato, Tokushi
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2019-04293

    Non-cryogenic structure of a chloride pump provides crucial clues to temperature-dependent channel transport efficiency
    journal, November 2018

    • Yun, Ji-Hye; Li, Xuanxuan; Park, Jae-Hyun
    • Journal of Biological Chemistry, Vol. 294, Issue 3
    • DOI: 10.1074/jbc.ra118.004038

    Inference-assisted intelligent crystallography based on preliminary data
    journal, August 2019

    • Hoshino, Manabu; Nakanishi-Ohno, Yoshinori; Hashizume, Daisuke
    • Scientific Reports, Vol. 9, Issue 1
    • DOI: 10.1038/s41598-019-48362-3

    Recent innovations in membrane-protein structural biology
    journal, January 2019

    The expanding toolkit for structural biology: synchrotrons, X-ray lasers and cryoEM
    journal, March 2019

    A unified formulation of the constant temperature molecular dynamics methods
    journal, July 1984

    • Nosé, Shuichi
    • The Journal of Chemical Physics, Vol. 81, Issue 1
    • DOI: 10.1063/1.447334

    Ultrafast photoisomerisation of an isolated retinoid
    journal, January 2019

    • Bull, James N.; West, Christopher W.; Anstöter, Cate S.
    • Physical Chemistry Chemical Physics, Vol. 21, Issue 20
    • DOI: 10.1039/c9cp01624d

    Viscosity-adjustable grease matrices for serial nanocrystallography
    journal, January 2020

    An outlook on using serial femtosecond crystallography in drug discovery
    journal, May 2019

    Pumping mechanism of NM-R3, a light-driven bacterial chloride importer in the rhodopsin family
    journal, February 2020

    Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography
    journal, July 2019

    Acid–base equilibrium of the chromophore counterion results in distinct photoisomerization reactivity in the primary event of proteorhodopsin
    journal, January 2019

    • Chang, Chun-Fu; Kuramochi, Hikaru; Singh, Manish
    • Physical Chemistry Chemical Physics, Vol. 21, Issue 46
    • DOI: 10.1039/c9cp04991f

    ClickX : a visualization-based program for preprocessing of serial crystallography data
    journal, May 2019

    • Li, Xuanxuan; Li, Chufeng; Liu, Haiguang
    • Journal of Applied Crystallography, Vol. 52, Issue 3
    • DOI: 10.1107/s1600576719005363

    Temperature-jump solution X-ray scattering reveals distinct motions in a dynamic enzyme
    journal, September 2019

    • Thompson, Michael C.; Barad, Benjamin A.; Wolff, Alexander M.
    • Nature Chemistry, Vol. 11, Issue 11
    • DOI: 10.1038/s41557-019-0329-3

    Strategies for sample delivery for femtosecond crystallography
    text, January 2019

    Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy
    journal, February 2020

    • Woodhouse, Joyce; Nass Kovacs, Gabriela; Coquelle, Nicolas
    • Nature Communications, Vol. 11, Issue 1
    • DOI: 10.1038/s41467-020-14537-0

    The cryo-EM method microcrystal electron diffraction (MicroED)
    journal, April 2019

    Strategies for sample delivery for femtosecond crystallography
    journal, February 2019

    • Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E.
    • Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2
    • DOI: 10.1107/s2059798318017953

    Time-resolved serial femtosecond crystallography at the European XFEL
    journal, November 2019

    Ultracompact 3D microfluidics for time-resolved structural biology
    journal, January 2020

    Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
    journal, July 2019

    • Nass Kovacs, Gabriela; Colletier, Jacques-Philippe; Grünbein, Marie Luise
    • Nature Communications, Vol. 10, Issue 1
    • DOI: 10.1038/s41467-019-10758-0

    Ultracompact 3D microfluidics for time-resolved structural biology
    text, January 2020

    • Knoška, Juraj; Adriano, Luigi; Awel, Salah
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00687

    Collective exchange processes reveal an active site proton cage in bacteriorhodopsin
    text, January 2020

    • Friedrich, Daniel; Brünig, Florian N.; Nieuwkoop, Andrew J.
    • Freie Universität Berlin
    • DOI: 10.17169/refubium-26480

    Ultracompact 3D microfluidics for time-resolved structural biology
    text, January 2020

    • Knoska, Juraj; Adriano, Luigi; Awel, Salah
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2020-00623

    Catalytically important damage-free structures of a copper nitrite reductase obtained by femtosecond X-ray laser and room-temperature neutron crystallography
    text, January 2019

    Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin
    text, January 2019

      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections:

      Other research related to this record: