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Title: Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles

Abstract

The development of dental adhesive resins with long-lasting antibacterial properties is a possible solution to overcome the problem of secondary caries in modern adhesive dentistry.Objectives: (i) Synthesis and characterization of nitrogen-doped titanium dioxide nanoparticles (N_TiO 2), (ii) topographical, compositional and wettability characterization of thin-films (unaltered and experimental) and, (iii) antibacterial efficacy of N_TiO 2-containing dental adhesives against Streptococcus mutans biofilms.Materials and methods: Nanoparticles were synthesized and characterized using different techniques. Specimens (diameter = 12 mm, thickness ≅ 15 μm) of OptiBond Solo Plus (Kerr Corp., USA) and experimental adhesives [50, 67 and 80% (v/v)] were fabricated, photopolymerized (1000 mW/cm 2, 1 min) and UV-sterilized (254 nm, 800,000 μJ/cm 2) for microscopy, spectroscopy, wettability and antibacterial testing. Wettability was assessed with a contact angle goniometer by dispensing water droplets (2 μL) onto four random locations of each specimen (16 drops/group). Drop profiles were recorded (1 min, 25 frames/s, 37 °C) and contact angles were calculated at time = 0 s (θ INITIAL) and time = 59 s (θ FINAL). Antibacterial testing was performed by growing S. mutans (UA159-ldh, JM10) biofilms for either 3 or 24 h (anaerobic conditions, 37 °C) with or without continuous light irradiation (410 ± 10 nm, 3more » h = 38.75 J/cm 2, 24 h = 310.07 J/cm 2) against the surfaces of sterile specimens.Results: N_TiO 2 was successfully prepared using solvothermal methods. Doped-nanoparticles displayed higher light absorption levels when compared to undoped titania. Experimental adhesives demonstrated superior antibacterial efficacy in dark conditions.Conclusions: The findings presented herein suggest that N_TiO 2 is a feasible antibacterial agent against cariogenic biofilms.« less

Authors:
ORCiD logo [1];  [1];  [2];  [2];  [2];  [3];  [1]
  1. Univ. of Oklahoma Health Sciences Center, Oklahoma City, OK (United States)
  2. Univ. of Oklahoma, Norman, OK (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1470843
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems
Additional Journal Information:
Journal Volume: 93; Journal Issue: C; Journal ID: ISSN 0928-4931
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Esteban Florez, Fernando Luis, Hiers, Rochelle Denise, Larson, Preston, Johnson, Matthew, O'Rear, Edgar, Rondinone, Adam J., and Khajotia, Sharukh Soli. Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles. United States: N. p., 2018. Web. doi:10.1016/j.msec.2018.08.060.
Esteban Florez, Fernando Luis, Hiers, Rochelle Denise, Larson, Preston, Johnson, Matthew, O'Rear, Edgar, Rondinone, Adam J., & Khajotia, Sharukh Soli. Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles. United States. doi:10.1016/j.msec.2018.08.060.
Esteban Florez, Fernando Luis, Hiers, Rochelle Denise, Larson, Preston, Johnson, Matthew, O'Rear, Edgar, Rondinone, Adam J., and Khajotia, Sharukh Soli. Sat . "Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles". United States. doi:10.1016/j.msec.2018.08.060. https://www.osti.gov/servlets/purl/1470843.
@article{osti_1470843,
title = {Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles},
author = {Esteban Florez, Fernando Luis and Hiers, Rochelle Denise and Larson, Preston and Johnson, Matthew and O'Rear, Edgar and Rondinone, Adam J. and Khajotia, Sharukh Soli},
abstractNote = {The development of dental adhesive resins with long-lasting antibacterial properties is a possible solution to overcome the problem of secondary caries in modern adhesive dentistry.Objectives: (i) Synthesis and characterization of nitrogen-doped titanium dioxide nanoparticles (N_TiO2), (ii) topographical, compositional and wettability characterization of thin-films (unaltered and experimental) and, (iii) antibacterial efficacy of N_TiO2-containing dental adhesives against Streptococcus mutans biofilms.Materials and methods: Nanoparticles were synthesized and characterized using different techniques. Specimens (diameter = 12 mm, thickness ≅ 15 μm) of OptiBond Solo Plus (Kerr Corp., USA) and experimental adhesives [50, 67 and 80% (v/v)] were fabricated, photopolymerized (1000 mW/cm2, 1 min) and UV-sterilized (254 nm, 800,000 μJ/cm2) for microscopy, spectroscopy, wettability and antibacterial testing. Wettability was assessed with a contact angle goniometer by dispensing water droplets (2 μL) onto four random locations of each specimen (16 drops/group). Drop profiles were recorded (1 min, 25 frames/s, 37 °C) and contact angles were calculated at time = 0 s (θINITIAL) and time = 59 s (θFINAL). Antibacterial testing was performed by growing S. mutans (UA159-ldh, JM10) biofilms for either 3 or 24 h (anaerobic conditions, 37 °C) with or without continuous light irradiation (410 ± 10 nm, 3 h = 38.75 J/cm2, 24 h = 310.07 J/cm2) against the surfaces of sterile specimens.Results: N_TiO2 was successfully prepared using solvothermal methods. Doped-nanoparticles displayed higher light absorption levels when compared to undoped titania. Experimental adhesives demonstrated superior antibacterial efficacy in dark conditions.Conclusions: The findings presented herein suggest that N_TiO2 is a feasible antibacterial agent against cariogenic biofilms.},
doi = {10.1016/j.msec.2018.08.060},
journal = {Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems},
number = C,
volume = 93,
place = {United States},
year = {2018},
month = {9}
}

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