skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methanobactin and the Link between Copper and Bacterial Methane Oxidation

Abstract

Methanobactins (mbs) are low-molecular-mass (<1,200 Da) copper-binding peptides, or chalkophores, produced by many methane-oxidizing bacteria (methanotrophs). These molecules exhibit similarities to certain iron-binding siderophores but are expressed and secreted in response to copper limitation. Structurally, mbs are characterized by a pair of heterocyclic rings with associated thioamide groups that form the copper coordination site. One of the rings is always an oxazolone and the second ring an oxazolone, an imidazolone, or a pyrazinedione moiety. The mb molecule originates from a peptide precursor that undergoes a series of posttranslational modifications, including (i) ring formation, (ii) cleavage of a leader peptide sequence, and (iii) in some cases, addition of a sulfate group. Functionally, mbs represent the extracellular component of a copper acquisition system. Consistent with this role in copper acquisition, mbs have a high affinity for copper ions. Following binding, mbs rapidly reduce Cu2+ to Cu1+. In addition to binding copper, mbs will bind most transition metals and near-transition metals and protect the host methanotroph as well as other bacteria from toxic metals. Several other physiological functions have been assigned to mbs, based primarily on their redox and metal-binding properties. Here, we examine the current state of knowledge of this novel typemore » of metal-binding peptide. We also explore its potential applications, how mbs may alter the bioavailability of multiple metals, and the many roles mbs may play in the physiology of methanotrophs.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6]
  1. Iowa State Univ., Ames, IA (United States). Roy J. Carver Dept. of Biochemistry, Biophysics and Molecular Biology
  2. Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Civil and Environmental Engineering
  3. Univ. of East Anglia, Norwich (United Kingdom). Earth and Life Systems Alliance and School of Environmental Sciences
  4. Univ. of Wisconsin, Eau Claire, WI (United States). Dept. of Chemistry
  5. Newcastle Univ., Newcastle upon Tyne (United Kingdom). Inst. for Cell and Molecular Biosciences
  6. Univ. of Strasbourg, UMR 7156 UNISTRA-CNRS, (France). Dept. of Microbiology, Genomics and the Environment
Publication Date:
Research Org.:
Univ. of Michigan, Ann Arbor, MI (United States).
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23; National Science Foundation (NSF); Natural Environment Research Council (NERC); Biotechnology and Biological Sciences Research Council (BBSRC)
OSTI Identifier:
1470738
Grant/Contract Number:  
SC0006630; CHE10112271; NE/F00608X; BB/K008439/1
Resource Type:
Accepted Manuscript
Journal Name:
Microbiology and Molecular Biology Reviews
Additional Journal Information:
Journal Volume: 80; Journal Issue: 2; Journal ID: ISSN 1092-2172
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

DiSpirito, Alan A., Semrau, Jeremy D., Murrell, J. Colin, Gallagher, Warren H., Dennison, Christopher, and Vuilleumier, Stéphane. Methanobactin and the Link between Copper and Bacterial Methane Oxidation. United States: N. p., 2016. Web. doi:10.1128/MMBR.00058-15.
DiSpirito, Alan A., Semrau, Jeremy D., Murrell, J. Colin, Gallagher, Warren H., Dennison, Christopher, & Vuilleumier, Stéphane. Methanobactin and the Link between Copper and Bacterial Methane Oxidation. United States. doi:10.1128/MMBR.00058-15.
DiSpirito, Alan A., Semrau, Jeremy D., Murrell, J. Colin, Gallagher, Warren H., Dennison, Christopher, and Vuilleumier, Stéphane. Wed . "Methanobactin and the Link between Copper and Bacterial Methane Oxidation". United States. doi:10.1128/MMBR.00058-15. https://www.osti.gov/servlets/purl/1470738.
@article{osti_1470738,
title = {Methanobactin and the Link between Copper and Bacterial Methane Oxidation},
author = {DiSpirito, Alan A. and Semrau, Jeremy D. and Murrell, J. Colin and Gallagher, Warren H. and Dennison, Christopher and Vuilleumier, Stéphane},
abstractNote = {Methanobactins (mbs) are low-molecular-mass (<1,200 Da) copper-binding peptides, or chalkophores, produced by many methane-oxidizing bacteria (methanotrophs). These molecules exhibit similarities to certain iron-binding siderophores but are expressed and secreted in response to copper limitation. Structurally, mbs are characterized by a pair of heterocyclic rings with associated thioamide groups that form the copper coordination site. One of the rings is always an oxazolone and the second ring an oxazolone, an imidazolone, or a pyrazinedione moiety. The mb molecule originates from a peptide precursor that undergoes a series of posttranslational modifications, including (i) ring formation, (ii) cleavage of a leader peptide sequence, and (iii) in some cases, addition of a sulfate group. Functionally, mbs represent the extracellular component of a copper acquisition system. Consistent with this role in copper acquisition, mbs have a high affinity for copper ions. Following binding, mbs rapidly reduce Cu2+ to Cu1+. In addition to binding copper, mbs will bind most transition metals and near-transition metals and protect the host methanotroph as well as other bacteria from toxic metals. Several other physiological functions have been assigned to mbs, based primarily on their redox and metal-binding properties. Here, we examine the current state of knowledge of this novel type of metal-binding peptide. We also explore its potential applications, how mbs may alter the bioavailability of multiple metals, and the many roles mbs may play in the physiology of methanotrophs.},
doi = {10.1128/MMBR.00058-15},
journal = {Microbiology and Molecular Biology Reviews},
number = 2,
volume = 80,
place = {United States},
year = {2016},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 30 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Effects of O2 and CH4 on presence and activity of the indigenous methanotrophic community in rice field soil
journal, December 2000


Methanotrophs and copper
journal, July 2010


Soil Methane and Carbon Dioxide Fluxes from Cropland and Riparian Buffers in Different Hydrogeomorphic Settings
journal, July 2015

  • Jacinthe, P. A.; Vidon, P.; Fisher, K.
  • Journal of Environmental Quality, Vol. 44, Issue 4
  • DOI: 10.2134/jeq2015.01.0014

Biosynthesis of secondary metabolites in methanotrophs: Biochemical and genetic aspects (Review)
journal, March 2015

  • Khmelenina, V. N.; Rozova, O. N.; But, S. Yu.
  • Applied Biochemistry and Microbiology, Vol. 51, Issue 2
  • DOI: 10.1134/S0003683815020088

Methane as a Resource: Can the Methanotrophs Add Value?
journal, March 2015

  • Strong, P. J.; Xie, S.; Clarke, W. P.
  • Environmental Science & Technology, Vol. 49, Issue 7
  • DOI: 10.1021/es504242n

Studies on some methane-utilizing bacteria
journal, January 1958

  • Leadbetter, Edward R.; Foster, J. W.
  • Archiv f�r Mikrobiologie, Vol. 30, Issue 1
  • DOI: 10.1007/BF00509229

Enrichment, Isolation and Some Properties of Methane-utilizing Bacteria
journal, May 1970

  • Whittenbury, R.; Phillips, K. C.; Wilkinson, J. F.
  • Journal of General Microbiology, Vol. 61, Issue 2
  • DOI: 10.1099/00221287-61-2-205

Methylomarinum vadi gen. nov., sp. nov., a methanotroph isolated from two distinct marine environments
journal, June 2012

  • Hirayama, H.; Fuse, H.; Abe, M.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 63, Issue Pt 3
  • DOI: 10.1099/ijs.0.040568-0

Methylomarinovum caldicuralii gen. nov., sp. nov., a moderately thermophilic methanotroph isolated from a shallow submarine hydrothermal system, and proposal of the family Methylothermaceae fam. nov.
journal, December 2013

  • Hirayama, H.; Abe, M.; Miyazaki, M.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 64, Issue Pt 3
  • DOI: 10.1099/ijs.0.058172-0

Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the 'deep sea-1' clade of marine methanotrophs
journal, October 2014

  • Tavormina, P. L.; Hatzenpichler, R.; McGlynn, S.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 65, Issue Pt 1
  • DOI: 10.1099/ijs.0.062927-0

Methyloparacoccus murrellii gen. nov., sp. nov., a methanotroph isolated from pond water
journal, March 2014

  • Hoefman, S.; van der Ha, D.; Iguchi, H.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 64, Issue Pt 6
  • DOI: 10.1099/ijs.0.057760-0

Draft Genome Sequence of the Methanotrophic Gammaproteobacterium Methyloglobulus morosus DSM 22980 Strain KoM1
journal, October 2013


Methyloferula stellata gen. nov., sp. nov., an acidophilic, obligately methanotrophic bacterium that possesses only a soluble methane monooxygenase
journal, November 2010

  • Vorobev, A. V.; Baani, M.; Doronina, N. V.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 61, Issue 10
  • DOI: 10.1099/ijs.0.028118-0

Acetate utilization as a survival strategy of peat-inhabiting Methylocystis spp.: Acetate utilization by Methylocystis spp.
journal, February 2011


Methylocella Species Are Facultatively Methanotrophic
journal, June 2005


Methylocapsa aurea sp. nov., a facultative methanotroph possessing a particulate methane monooxygenase, and emended description of the genus Methylocapsa
journal, January 2010

  • Dunfield, P. F.; Belova, S. E.; Vorob'ev, A. V.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 11
  • DOI: 10.1099/ijs.0.020149-0

Facultative methanotrophy: false leads, true results, and suggestions for future research: Facultative methanotrophy
journal, June 2011


The genomic landscape of the verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV
journal, January 2014


Nitrite-driven anaerobic methane oxidation by oxygenic bacteria
journal, March 2010

  • Ettwig, Katharina F.; Butler, Margaret K.; Le Paslier, Denis
  • Nature, Vol. 464, Issue 7288
  • DOI: 10.1038/nature08883

The Leeuwenhoek Lecture 2000 The natural and unnatural history of methane-oxidizing bacteria
journal, June 2005

  • Dalton, Howard
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 360, Issue 1458
  • DOI: 10.1098/rstb.2005.1657

Molecular genetics of methane oxidation
journal, December 1994


Regulation of expression of methane monooxygenases by copper ions
journal, May 2000


The Biochemistry of Methane Oxidation
journal, June 2007


Evolution of the soluble diiron monooxygenases
journal, October 2003


Moessbauer, EPR, and ENDOR studies of the hydroxylase and reductase components of methane monooxygenase from Methylosinus trichosporium OB3b
journal, May 1993

  • Fox, Brian G.; Hendrich, Michael P.; Surerus, Kristene K.
  • Journal of the American Chemical Society, Vol. 115, Issue 9
  • DOI: 10.1021/ja00062a039

Dioxygen Activation by Enzymes Containing Binuclear Non-Heme Iron Clusters
journal, January 1996

  • Wallar, Bradley J.; Lipscomb, John D.
  • Chemical Reviews, Vol. 96, Issue 7
  • DOI: 10.1021/cr9500489

Crystallization and preliminary X-ray analysis of the methane monooxygenase hydroxylase protein from Methylococcus capsulatus (Bath)
journal, September 1992

  • Rosenzweig, Amy C.; Frederick, Christin A.; Lippard, Stephen J.
  • Journal of Molecular Biology, Vol. 227, Issue 2
  • DOI: 10.1016/0022-2836(92)90913-5

Product Bound Structures of the Soluble Methane Monooxygenase Hydroxylase from Methylococcus capsulatus (Bath):  Protein Motion in the α-Subunit
journal, April 2005

  • Sazinsky, Matthew H.; Lippard, Stephen J.
  • Journal of the American Chemical Society, Vol. 127, Issue 16
  • DOI: 10.1021/ja044099b

Structure of the soluble methane monooxygenase regulatory protein B
journal, July 1999

  • Walters, K. J.; Gassner, G. T.; Lippard, S. J.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 14
  • DOI: 10.1073/pnas.96.14.7877

Solution Structure of Component B from Methane Monooxygenase Derived through Heteronuclear NMR and Molecular Modeling ,
journal, May 1999

  • Chang, Shou-Lin; Wallar, Bradley J.; Lipscomb, John D.
  • Biochemistry, Vol. 38, Issue 18
  • DOI: 10.1021/bi982992f

NMR Structure of the Flavin Domain from Soluble Methane Monooxygenase Reductase from Methylococcus capsulatus (Bath) † , ‡
journal, September 2004

  • Chatwood, Lisa L.; Müller, Jens; Gross, John D.
  • Biochemistry, Vol. 43, Issue 38
  • DOI: 10.1021/bi049066n

Methane Monooxygenase Hydroxylase and B Component Interactions
journal, March 2006

  • Zhang, Jingyan; Wallar, Bradley J.; Popescu, Codrina V.
  • Biochemistry, Vol. 45, Issue 9
  • DOI: 10.1021/bi052256t

A transient intermediate of the methane monooxygenase catalytic cycle containing an FeIVFeIV cluster
journal, July 1993

  • Lee, Sang Kyu; Fox, Brian G.; Froland, Wayne A.
  • Journal of the American Chemical Society, Vol. 115, Issue 14
  • DOI: 10.1021/ja00067a086

Characterization of a Diiron(III) Peroxide Intermediate in the Reaction Cycle of Methane Monooxygenase Hydroxylase from Methylococcus capsulatus (Bath)
journal, May 1995

  • Liu, Katherine E.; Valentine, Ann M.; Qiu, Di
  • Journal of the American Chemical Society, Vol. 117, Issue 17
  • DOI: 10.1021/ja00122a032

Spectroscopic detection of intermediates in the reaction of dioxygen with the reduced methane monooxygenase/hydroxylase from Methylococcus capsulatus (Bath)
journal, August 1994

  • Liu, Katherine E.; Wang, Danli; Huynh, Boi Hanh
  • Journal of the American Chemical Society, Vol. 116, Issue 16
  • DOI: 10.1021/ja00095a083

Structure of the key species in the enzymatic oxidation of methane to methanol
journal, January 2015

  • Banerjee, Rahul; Proshlyakov, Yegor; Lipscomb, John D.
  • Nature, Vol. 518, Issue 7539
  • DOI: 10.1038/nature14160

Biochemistry of the Soluble Methane Monooxygenase
journal, October 1994


Quantum chemical studies of methane monooxygenase: comparision with P450
journal, April 2002

  • Guallar, Victor; Gherman, Benjamin F.; Lippard, Stephen J.
  • Current Opinion in Chemical Biology, Vol. 6, Issue 2
  • DOI: 10.1016/S1367-5931(02)00310-1

The membrane-associated form of methane mono-oxygenase from Methylococcus capsulatus (Bath) is a copper/iron protein
journal, January 2003

  • Basu, Piku; Katterle, Bettina; Andersson, K. Kristoffer
  • Biochemical Journal, Vol. 369, Issue 2
  • DOI: 10.1042/bj20020823

The Membrane-Associated Methane Monooxygenase (pMMO) and pMMO-NADH:Quinone Oxidoreductase Complex from Methylococcus capsulatus Bath
journal, September 2003


Characterization of the Particulate Methane Monooxygenase Metal Centers in Multiple Redox States by X-ray Absorption Spectroscopy
journal, October 2006

  • Lieberman, Raquel L.; Kondapalli, Kalyan C.; Shrestha, Deepak B.
  • Inorganic Chemistry, Vol. 45, Issue 20
  • DOI: 10.1021/ic060739v

Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane
journal, January 2005

  • Lieberman, Raquel L.; Rosenzweig, Amy C.
  • Nature, Vol. 434, Issue 7030
  • DOI: 10.1038/nature03311

Purified particulate methane monooxygenase from Methylococcus capsulatus (Bath) is a dimer with both mononuclear copper and a copper-containing cluster
journal, March 2003

  • Lieberman, R. L.; Shrestha, D. B.; Doan, P. E.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 7
  • DOI: 10.1073/pnas.0536703100

Mössbauer Studies of the Membrane-Associated Methane Monooxygenase from Methylococcus capsulatus Bath:  Evidence for a Diiron Center
journal, December 2007

  • Martinho, Marlène; Choi, Dong W.; DiSpirito, Alan A.
  • Journal of the American Chemical Society, Vol. 129, Issue 51
  • DOI: 10.1021/ja077682b

The Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) Is a Novel Copper-containing Three-subunit Enzyme : ISOLATION AND CHARACTERIZATION
journal, April 1998

  • Nguyen, Hiep-Hoa T.; Elliott, Sean J.; Yip, John Hon-Kay
  • Journal of Biological Chemistry, Vol. 273, Issue 14
  • DOI: 10.1074/jbc.273.14.7957

Purification and properties of particulate methane monooxygenase from Methylosinus trichosporium OB3b
journal, June 1998

  • Takeguchi, Masayuki; Miyakawa, Kyoko; Okura, Ichiro
  • Journal of Molecular Catalysis A: Chemical, Vol. 132, Issue 2-3
  • DOI: 10.1016/S1381-1169(97)00275-6

Membrane-associated methane monooxygenase from Methylococcus capsulatus (Bath).
journal, February 1996


The Metal Centers of Particulate Methane Monooxygenase from Methylosinus trichosporium OB3b
journal, July 2008

  • Hakemian, Amanda S.; Kondapalli, Kalyan C.; Telser, Joshua
  • Biochemistry, Vol. 47, Issue 26
  • DOI: 10.1021/bi800598h

Identification of the Valence and Coordination Environment of the Particulate Methane Monooxygenase Copper Centers by Advanced EPR Characterization
journal, August 2014

  • Culpepper, Megen A.; Cutsail III, George E.; Gunderson, William A.
  • Journal of the American Chemical Society, Vol. 136, Issue 33
  • DOI: 10.1021/ja5053126

Isolation of Intracytoplasmic Membrane from the Methanotrophic Bacterium Methylomicrobium album BG8
journal, February 2000

  • Brantner, Christine A.; Buchholz, Lorie A.; Remsen, Charles C.
  • Current Microbiology, Vol. 40, Issue 2
  • DOI: 10.1007/s002849910026

Particulate methane monooxygenase genes in methanotrophs.
journal, January 1995


Expression of Individual Copies of Methylococcus capsulatus Bath Particulate Methane Monooxygenase Genes
journal, March 2001


Three-Dimensional Structure Determination of a Protein Supercomplex That Oxidizes Methane to Formaldehyde in Methylococcus capsulatus (Bath)
journal, October 2006

  • Myronova, Natalia; Kitmitto, Ashraf; Collins, Richard F.
  • Biochemistry, Vol. 45, Issue 39
  • DOI: 10.1021/bi061294p

Structure and Protein–Protein Interactions of Methanol Dehydrogenase from Methylococcus capsulatus (Bath)
journal, September 2014

  • Culpepper, Megen A.; Rosenzweig, Amy C.
  • Biochemistry, Vol. 53, Issue 39
  • DOI: 10.1021/bi500850j

Electron transfer during the oxidation of ammonia by the chemolithotrophic bacterium Nitrosomonas europaea
journal, August 2000

  • Whittaker, Mark; Bergmann, David; Arciero, David
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1459, Issue 2-3
  • DOI: 10.1016/S0005-2728(00)00171-7

Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol
journal, September 2003

  • Dunfield, P. F.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 53, Issue 5
  • DOI: 10.1099/ijs.0.02481-0

Methanol oxidation genes in the marine methanotroph Methylomonas sp. strain A4.
journal, January 1993


The structure of bacterial quinoprotein dehydrogenases
journal, January 1992


Molecular cloning of the methanol dehydrogenase structural gene fromMethylosinus trichosporium OB3b
journal, March 1990

  • Al-Taho, N. M.; Cornish, A.; Warner, P. J.
  • Current Microbiology, Vol. 20, Issue 3
  • DOI: 10.1007/BF02091990

Rare earth metals are essential for methanotrophic life in volcanic mudpots: Rare earth metals essential for methanotrophic life
journal, September 2013

  • Pol, Arjan; Barends, Thomas R. M.; Dietl, Andreas
  • Environmental Microbiology, Vol. 16, Issue 1
  • DOI: 10.1111/1462-2920.12249

Cerium Regulates Expression of Alternative Methanol Dehydrogenases in Methylosinus trichosporium OB3b
journal, August 2015

  • Farhan Ul Haque, Muhammad; Kalidass, Bhagyalakshmi; Bandow, Nathan
  • Applied and Environmental Microbiology, Vol. 81, Issue 21
  • DOI: 10.1128/AEM.02542-15

Membrane-Associated Quinoprotein Formaldehyde Dehydrogenase from Methylococcus capsulatus Bath
journal, December 2001


Cofactor-dependent pathways of formaldehyde oxidation in methylotrophic bacteria
journal, October 2002


Purification and Properties of an NAD(P)+-linked Formaldehyde Dehydrogenase from Methylococcus capsulatus (Bath)
journal, July 1978


Sequence of the gene for a NAD(P)-dependent formaldehyde dehydrogenase (class III alcohol dehydrogenase) from a marine methanotroph Methylobacter marinus A45
journal, September 1994


Formate dehydrogenase from the methane oxidizer Methylosinus trichosporium OB3b.
journal, January 1990


Properties and partial purification of the methane-oxidising enzyme system from Methylosinus trichosporium
journal, October 1975


Methane oxidation by cell-free extracts of Methylococcus capsulatus
journal, November 1970


A comparison of the substrate and electron-donor specificities of the methane mono-oxygenases from three strains of methane-oxidizing bacteria
journal, January 1979

  • Stirling, D. I.; Colby, J.; Dalton, H.
  • Biochemical Journal, Vol. 177, Issue 1
  • DOI: 10.1042/bj1770361

Copper ions as inhibitors of protein C of soluble methane monooxygenase of Methylococcus capsulatus (Bath)
journal, November 1985


Ultrastructure of intracytoplasmic membranes of Methanomonas margaritae cells grown under different conditions
journal, January 1980

  • Takeda, Kiyoshi; Tanaka, Kenji
  • Antonie van Leeuwenhoek, Vol. 46, Issue 1
  • DOI: 10.1007/BF00422225

Copper-dependent reciprocal transcriptional regulation of methane monooxygenase genes in Methylococcus capsulatus and Methylosinus trichosporium
journal, July 1997


rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b
journal, July 2003

  • Stafford, Graham P.; Scanlan, Julie; McDonald, Ian R.
  • Microbiology, Vol. 149, Issue 7
  • DOI: 10.1099/mic.0.26060-0

Quantitative Proteomic Analysis of Metabolic Regulation by Copper Ions in Methylococcus capsulatus (Bath)
journal, September 2004

  • Kao, Wei-Chun; Chen, Yet-Ran; Yi, Eugene C.
  • Journal of Biological Chemistry, Vol. 279, Issue 49
  • DOI: 10.1074/jbc.M408013200

Distribution and Selection of Poly-3-Hydroxybutyrate Production Capacity in Methanotrophic Proteobacteria
journal, May 2011

  • Pieja, Allison J.; Rostkowski, Katherine H.; Criddle, Craig S.
  • Microbial Ecology, Vol. 62, Issue 3
  • DOI: 10.1007/s00248-011-9873-0

The copper responding surfaceome of Methylococccus capsulatus Bath
journal, August 2011


CorA Is a Copper Repressible Surface-Associated Copper(I)-Binding Protein Produced in Methylomicrobium album BG8
journal, February 2014


A four-helix bundle stores copper for methane oxidation
journal, August 2015

  • Vita, Nicolas; Platsaki, Semeli; Baslé, Arnaud
  • Nature, Vol. 525, Issue 7567
  • DOI: 10.1038/nature14854

Spectral and thermodynamic properties of methanobactin from γ-proteobacterial methane oxidizing bacteria: A case for copper competition on a molecular level
journal, December 2010


Variations in methanobactin structure influences copper utilization by methane-oxidizing bacteria
journal, May 2012

  • El Ghazouani, A.; Basle, A.; Gray, J.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 22
  • DOI: 10.1073/pnas.1112921109

Methanobactin, a Copper-Acquisition Compound from Methane-Oxidizing Bacteria
journal, September 2004


Copper-Binding Properties and Structures of Methanobactins from Methylosinus trichosporium OB3b
journal, February 2011

  • El Ghazouani, Abdelnasser; Baslé, Arnaud; Firbank, Susan J.
  • Inorganic Chemistry, Vol. 50, Issue 4
  • DOI: 10.1021/ic101965j

Spectral and copper binding properties of methanobactin from the facultative methanotroph Methylocystis strain SB2
journal, May 2012


Siderophores: Structure and Function of Microbial Iron Transport Compounds
journal, November 1995


Draft Genome Sequence of Methylomicrobium buryatense Strain 5G, a Haloalkaline-Tolerant Methanotrophic Bacterium
journal, June 2013


Spectral, Kinetic, and Thermodynamic Properties of Cu(I) and Cu(II) Binding by Methanobactin from Methylosinus trichosporium OB3b
journal, February 2006

  • Choi, Dong W.; Zea, Corbin J.; Do, Young S.
  • Biochemistry, Vol. 45, Issue 5
  • DOI: 10.1021/bi051815t

The siderophore yersiniabactin binds copper to protect pathogens during infection
journal, July 2012

  • Chaturvedi, Kaveri S.; Hung, Chia S.; Crowley, Jan R.
  • Nature Chemical Biology, Vol. 8, Issue 8
  • DOI: 10.1038/nchembio.1020

Linkage between Catecholate Siderophores and the Multicopper Oxidase CueO in Escherichia coli
journal, August 2004


An assay for screening microbial cultures for chalkophore production
journal, April 2010


NMR, Mass Spectrometry and Chemical Evidence Reveal a Different Chemical Structure for Methanobactin That Contains Oxazolone Rings
journal, September 2008

  • Behling, Lee A.; Hartsel, Scott C.; Lewis, David E.
  • Journal of the American Chemical Society, Vol. 130, Issue 38
  • DOI: 10.1021/ja804747d

Purification and Physical−Chemical Properties of Methanobactin:  A Chalkophore from Methylosinus trichosporium OB3b
journal, April 2005

  • Kim, Hyung J.; Galeva, Nadezhda; Larive, Cynthia K.
  • Biochemistry, Vol. 44, Issue 13
  • DOI: 10.1021/bi047367r

Thioamides in Nature: In Search of Secondary Metabolites in Anaerobic Microorganisms
journal, May 2010


Mercury binding by methanobactin from Methylocystis strain SB2
journal, December 2014


Excitonic connectivity between photosystem II units: what is it, and how to measure it?
journal, June 2013


Circular Dichroism of Carotenoids in Bacterial Light-Harvesting Complexes: Experiments and Modeling
journal, November 2004


Absorption and CD Spectroscopy and Modeling of Various LH2 Complexes from Purple Bacteria
journal, April 2002


Formation of a Dinuclear Copper(I) Complex from the Clostridium -Derived Antibiotic Closthioamide
journal, August 2013

  • Kloss, Florian; Pidot, Sacha; Goerls, Helmar
  • Angewandte Chemie International Edition, Vol. 52, Issue 41
  • DOI: 10.1002/anie.201304714

A genomic approach to the cryptic secondary metabolome of the anaerobic world
journal, January 2013

  • Letzel, Anne-Catrin; Pidot, Sacha J.; Hertweck, Christian
  • Nat. Prod. Rep., Vol. 30, Issue 3
  • DOI: 10.1039/C2NP20103H

Closthioamide: An Unprecedented Polythioamide Antibiotic from the Strictly Anaerobic Bacterium Clostridium cellulolyticum
journal, February 2010

  • Lincke, Thorger; Behnken, Swantje; Ishida, Keishi
  • Angewandte Chemie International Edition, Vol. 49, Issue 11
  • DOI: 10.1002/anie.200906114

Is coproporphyrin III a copper-acquisition compound in Paracoccus denitrificans?
journal, March 2011

  • Anttila, Jani; Heinonen, Petri; Nenonen, Timo
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1807, Issue 3
  • DOI: 10.1016/j.bbabio.2010.12.014

From Peptide Precursors to Oxazole and Thiazole-Containing Peptide Antibiotics: Microcin B17 Synthase
journal, November 1996


Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature
journal, January 2013

  • Arnison, Paul G.; Bibb, Mervyn J.; Bierbaum, Gabriele
  • Nat. Prod. Rep., Vol. 30, Issue 1
  • DOI: 10.1039/C2NP20085F

Thiazole/oxazole-modified microcins: complex natural products from ribosomal templates
journal, June 2011

  • Melby, Joel O.; Nard, Nathan J.; Mitchell, Douglas A.
  • Current Opinion in Chemical Biology, Vol. 15, Issue 3
  • DOI: 10.1016/j.cbpa.2011.02.027

A New 1-Hydroxy-2,6-pyrazinedione Associated with Hypovirulent Isolates of Sclerotinia minor
journal, February 2003

  • Savard, M. E.; Melzer, M. S.; Boland, G. J.
  • Journal of Natural Products, Vol. 66, Issue 2
  • DOI: 10.1021/np020445w

A novel antiviral agent which inhibits the endonuclease of influenza viruses.
journal, May 1996

  • Tomassini, J. E.; Davies, M. E.; Hastings, J. C.
  • Antimicrobial Agents and Chemotherapy, Vol. 40, Issue 5
  • DOI: 10.1128/AAC.40.5.1189

Thioviridamide, a Novel Apoptosis Inducer in Transformed Cells from Streptomyces olivoviridis
journal, January 2006

  • Hayakawa, Yoichi; Sasaki, Kahori; Adachi, Hisashi
  • The Journal of Antibiotics, Vol. 59, Issue 1
  • DOI: 10.1038/ja.2006.1

Structure of Thioviridamide, a Novel Apoptosis Inducer from Streptomyces olivoviridis
journal, January 2006

  • Hayakawa, Yoichi; Sasaki, Kahori; Nagai, Koji
  • The Journal of Antibiotics, Vol. 59, Issue 1
  • DOI: 10.1038/ja.2006.2

Copper complexation of methanobactin isolated from Methylosinus trichosporium OB3b: pH-dependent speciation and modeling
journal, November 2012


Robust Affinity Standards for Cu(I) Biochemistry
journal, November 2013

  • Bagchi, Pritha; Morgan, M. Thomas; Bacsa, John
  • Journal of the American Chemical Society, Vol. 135, Issue 49
  • DOI: 10.1021/ja408827d

Thermodynamics of copper and zinc distribution in the cyanobacterium Synechocystis PCC 6803
journal, July 2011

  • Badarau, Adriana; Dennison, Christopher
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 32
  • DOI: 10.1073/pnas.1101448108

Copper Trafficking Mechanism of CXXC-Containing Domains: Insight from the pH-Dependence of Their Cu(I) Affinities
journal, March 2011

  • Badarau, Adriana; Dennison, Christopher
  • Journal of the American Chemical Society, Vol. 133, Issue 9
  • DOI: 10.1021/ja1091547

Stimulation of Methanotroph Activity by Cu-Substituted Borosilicate Glass
journal, January 2011

  • Kulczycki, Ezra; Fowle, David A.; Kenward, Paul A.
  • Geomicrobiology Journal, Vol. 28, Issue 1
  • DOI: 10.1080/01490451003614971

Metal speciation and microbial growth--the hard (and soft) facts
journal, April 1991


A New Method to Measure Effective Soil Solution Concentration Predicts Copper Availability to Plants
journal, June 2001

  • Zhang, Hao; Zhao, Fang-Jie; Sun, Bo
  • Environmental Science & Technology, Vol. 35, Issue 12
  • DOI: 10.1021/es000268q

Fractionation of Cu, Pb and Zn in certified reference soils SRM 2710 and SRM 2711 using the optimized BCR sequential extraction procedure
journal, October 2003


Metal-Macrofauna Interactions Determine Microbial Community Structure and Function in Copper Contaminated Sediments
journal, May 2013


Solid-Solution Partitioning of Metals in Contaminated Soils:  Dependence on pH, Total Metal Burden, and Organic Matter
journal, April 2000

  • Sauvé, Sébastien; Hendershot, William; Allen, Herbert E.
  • Environmental Science & Technology, Vol. 34, Issue 7
  • DOI: 10.1021/es9907764

Detoxification of Mercury by Methanobactin from Methylosinus trichosporium OB3b
journal, July 2013

  • Vorobev, Alexey; Jagadevan, Sheeja; Baral, Bipin S.
  • Applied and Environmental Microbiology, Vol. 79, Issue 19
  • DOI: 10.1128/AEM.01673-13

The Copper Chelator Methanobactin from Methylosinus t richosporium OB3b Binds Copper(I)
journal, December 2005

  • Hakemian, Amanda S.; Tinberg, Christine E.; Kondapalli, Kalyan C.
  • Journal of the American Chemical Society, Vol. 127, Issue 49
  • DOI: 10.1021/ja0558140

Genome mining for methanobactins
journal, February 2013


Methanobactin and MmoD work in concert to act as the ‘copper-switch’ in methanotrophs: Copper-switch in methanotrophy
journal, May 2013


Catalytic promiscuity of a bacterial α- N -methyltransferase
journal, July 2012


Synthesis and Spectral and Chemical Properties of the Yellow Fluorescent Protein zFP538 Chromophore
journal, August 2009

  • Yampolsky, Ilia V.; Balashova, Tamara A.; Lukyanov, Konstantin A.
  • Biochemistry, Vol. 48, Issue 33
  • DOI: 10.1021/bi900719x

Dual Pathways for Copper Uptake by Methanotrophic Bacteria
journal, September 2011

  • Balasubramanian, Ramakrishnan; Kenney, Grace E.; Rosenzweig, Amy C.
  • Journal of Biological Chemistry, Vol. 286, Issue 43
  • DOI: 10.1074/jbc.M111.284984

Methane monooxygenase gene expression mediated by methanobactin in the presence of mineral copper sources
journal, July 2007

  • Knapp, C. W.; Fowle, D. A.; Kulczycki, E.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 29
  • DOI: 10.1073/pnas.0702879104

Effect of copper on membrane lipids and on methane monooxygenase activity of Methylococcus capsulatus (Bath)
journal, June 1993

  • Peltola, Petri; Priha, Petri; Laakso, Simo
  • Archives of Microbiology, Vol. 159, Issue 6
  • DOI: 10.1007/BF00249029

The biology of oxygen radicals
journal, September 1978


The Haber–Weiss reaction and mechanisms of toxicity
journal, August 2000


Oxidase, superoxide dismutase, and hydrogen peroxide reductase activities of methanobactin from types I and II methanotrophs
journal, August 2008


Copper Chelators: Chemical Properties and Bio-medical Applications
journal, June 2014


Gold Nanoparticles in Chemical and Biological Sensing
journal, February 2012

  • Saha, Krishnendu; Agasti, Sarit S.; Kim, Chaekyu
  • Chemical Reviews, Vol. 112, Issue 5
  • DOI: 10.1021/cr2001178

Alkanethiolate Gold Cluster Molecules with Core Diameters from 1.5 to 5.2 nm:  Core and Monolayer Properties as a Function of Core Size
journal, January 1998

  • Hostetler, Michael J.; Wingate, Julia E.; Zhong, Chuan-Jian
  • Langmuir, Vol. 14, Issue 1
  • DOI: 10.1021/la970588w

4-Hydroxythiophenol-Protected Gold Nanoclusters in Aqueous Media
journal, October 1999


Arenethiolate Monolayer-Protected Gold Clusters
journal, February 1999


Colorimetric detection of melamine based on methanobactin-mediated synthesis of gold nanoparticles
journal, May 2015


Methanobactin-Mediated Synthesis of Gold Nanoparticles Supported over Al2O3 toward an Efficient Catalyst for Glucose Oxidation
journal, November 2014

  • Xin, Jia-Ying; Lin, Kai; Wang, Yan
  • International Journal of Molecular Sciences, Vol. 15, Issue 12
  • DOI: 10.3390/ijms151221603

Production of nanoparticles using organisms
journal, October 2009

  • Korbekandi, Hassan; Iravani, Siavash; Abbasi, Sajjad
  • Critical Reviews in Biotechnology, Vol. 29, Issue 4, p. 279-306
  • DOI: 10.3109/07388550903062462

Surface-Enhanced Raman Spectroscopy (SERS) Cellular Imaging of Intracellulary Biosynthesized Gold Nanoparticles
journal, May 2014

  • Lahr, Rebecca Halvorson; Vikesland, Peter J.
  • ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 7
  • DOI: 10.1021/sc500105n

Wilson's disease
journal, February 2007


Wilson's disease
journal, October 2011


Molecular pathogenesis of Wilson and Menkes disease: correlation of mutations with molecular defects and disease phenotypes
journal, August 2007


Liver mitochondrial membrane crosslinking and destruction in a rat model of Wilson disease
journal, April 2011

  • Zischka, Hans; Lichtmannegger, Josef; Schmitt, Sabine
  • Journal of Clinical Investigation, Vol. 121, Issue 4
  • DOI: 10.1172/JCI45401

Wilson’s disease: Etiology, diagnosis, and treatment
journal, September 2014


The neurotoxicity of iron, copper and manganese in Parkinson's and Wilson's diseases
journal, July 2015

  • Dusek, Petr; Roos, Per M.; Litwin, Tomasz
  • Journal of Trace Elements in Medicine and Biology, Vol. 31
  • DOI: 10.1016/j.jtemb.2014.05.007

Metabolic fate of the insoluble copper/tetrathiomolybdate complex formed in the liver of LEC rats with excess tetrathiomolybdate
journal, January 2000


Mechanisms of selective copper removal by tetrathiomolybdate from metallothionein in LEC rats
journal, January 1996


The biogenic methanobactin is an effective chelator for copper in a rat model for Wilson disease
journal, January 2011

  • Summer, Karl H.; Lichtmannegger, Josef; Bandow, Nathan
  • Journal of Trace Elements in Medicine and Biology, Vol. 25, Issue 1
  • DOI: 10.1016/j.jtemb.2010.12.002

Targeting copper to treat breast cancer
journal, July 2015


Copper is required for oncogenic BRAF signalling and tumorigenesis
journal, April 2014

  • Brady, Donita C.; Crowe, Matthew S.; Turski, Michelle L.
  • Nature, Vol. 509, Issue 7501
  • DOI: 10.1038/nature13180

Methylomagnum ishizawai gen. nov., sp. nov., a mesophilic type I methanotroph isolated from rice rhizosphere
journal, October 2015

  • Khalifa, Ashraf; Dianou, Dayéri; Ueno, Chihoko
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 65, Issue 10
  • DOI: 10.1099/ijsem.0.000451

Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b
journal, January 2015

  • Farhan Ul-Haque, Muhammad; Kalidass, Bhagyalakshmi; Vorobev, Alexey
  • Applied and Environmental Microbiology, Vol. 81, Issue 7
  • DOI: 10.1128/AEM.03981-14

    Works referencing / citing this record:

    Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps
    journal, October 2019

    • Farhan Ul Haque, Muhammad; Crombie, Andrew T.; Murrell, J. Colin
    • Microbiome, Vol. 7, Issue 1
    • DOI: 10.1186/s40168-019-0741-3

    Rare Earth Elements Alter Redox Balance in Methylomicrobium alcaliphilum 20ZR
    journal, November 2018

    • Akberdin, Ilya R.; Collins, David A.; Hamilton, Richard
    • Frontiers in Microbiology, Vol. 9
    • DOI: 10.3389/fmicb.2018.02735

    Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps
    journal, October 2019

    • Farhan Ul Haque, Muhammad; Crombie, Andrew T.; Murrell, J. Colin
    • Microbiome, Vol. 7, Issue 1
    • DOI: 10.1186/s40168-019-0741-3