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Title: A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b

Abstract

Methanobactin, a small modified polypeptide synthesized by methanotrophs for copper uptake, has been found to be chromosomally encoded. The gene encoding the polypeptide precursor of methanobactin, mbnA, is part of a gene cluster that also includes several genes encoding proteins of unknown function (but speculated to be involved in methanobactin formation) as well as mbnT, which encodes a TonB-dependent transporter hypothesized to be responsible for methanobactin uptake. In order to determine if mbnT is truly responsible for methanobactin uptake, a knockout was constructed in Methylosinus trichosporium OB3b using marker exchange mutagenesis. The resulting M. trichosporium mbnT::Gmr mutant was found to be able to produce methanobactin but was unable to internalize it. Further, if this mutant was grown in the presence of copper and exogenous methanobactin, copper uptake was significantly reduced. Expression of mmoX and pmoA, encoding polypeptides of the soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO), respectively, also changed significantly when methanobactin was added, which indicates that the mutant was unable to collect copper under these conditions. Copper uptake and gene expression, however, were not affected in wild-type M. trichosporium OB3b, indicating that the TonB-dependent transporter encoded by mbnT is responsible for methanobactin uptake and that methanobactin ismore » a key mechanism used by methanotrophs for copper uptake. When the mbnT::Gmr mutant was grown under a range of copper concentrations in the absence of methanobactin, however, the phenotype of the mutant was indistinguishable from that of wild-type M. trichosporium OB3b, indicating that this methanotroph has multiple mechanisms for copper uptake.« less

Authors:
 [1];  [1];  [2];  [2];  [2];  [3];  [3];  [4];  [2];  [1]
  1. Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Civil and Environmental Engineering
  2. Iowa State Univ., Ames, IA (United States). Roy J. Carver Dept. of Biochemistry, Biophysics and Molecular Biology
  3. Helmholtz-Center Munich (HZM) (Germany). German Research Center for Environmental Health (HMGU) and Inst. of Molecular Immunology and Monoclonal Antibody Core Facility
  4. Helmholtz-Center Munich (HZM), Neuherberg (Germany). German Research Center for Environmental Health (HMGU) and Inst. of Molecular Toxicology and Pharmacology
Publication Date:
Research Org.:
Univ. of Michigan, Ann Arbor, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1470736
Grant/Contract Number:  
SC0006630
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 82; Journal Issue: 6; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Gu, Wenyu, Farhan Ul Haque, Muhammad, Baral, Bipin S., Turpin, Erick A., Bandow, Nathan L., Kremmer, Elisabeth, Flatley, Andrew, Zischka, Hans, DiSpirito, Alan A., and Semrau, Jeremy D. A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b. United States: N. p., 2016. Web. doi:10.1128/AEM.03884-15.
Gu, Wenyu, Farhan Ul Haque, Muhammad, Baral, Bipin S., Turpin, Erick A., Bandow, Nathan L., Kremmer, Elisabeth, Flatley, Andrew, Zischka, Hans, DiSpirito, Alan A., & Semrau, Jeremy D. A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b. United States. https://doi.org/10.1128/AEM.03884-15
Gu, Wenyu, Farhan Ul Haque, Muhammad, Baral, Bipin S., Turpin, Erick A., Bandow, Nathan L., Kremmer, Elisabeth, Flatley, Andrew, Zischka, Hans, DiSpirito, Alan A., and Semrau, Jeremy D. Mon . "A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b". United States. https://doi.org/10.1128/AEM.03884-15. https://www.osti.gov/servlets/purl/1470736.
@article{osti_1470736,
title = {A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b},
author = {Gu, Wenyu and Farhan Ul Haque, Muhammad and Baral, Bipin S. and Turpin, Erick A. and Bandow, Nathan L. and Kremmer, Elisabeth and Flatley, Andrew and Zischka, Hans and DiSpirito, Alan A. and Semrau, Jeremy D.},
abstractNote = {Methanobactin, a small modified polypeptide synthesized by methanotrophs for copper uptake, has been found to be chromosomally encoded. The gene encoding the polypeptide precursor of methanobactin, mbnA, is part of a gene cluster that also includes several genes encoding proteins of unknown function (but speculated to be involved in methanobactin formation) as well as mbnT, which encodes a TonB-dependent transporter hypothesized to be responsible for methanobactin uptake. In order to determine if mbnT is truly responsible for methanobactin uptake, a knockout was constructed in Methylosinus trichosporium OB3b using marker exchange mutagenesis. The resulting M. trichosporium mbnT::Gmr mutant was found to be able to produce methanobactin but was unable to internalize it. Further, if this mutant was grown in the presence of copper and exogenous methanobactin, copper uptake was significantly reduced. Expression of mmoX and pmoA, encoding polypeptides of the soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO), respectively, also changed significantly when methanobactin was added, which indicates that the mutant was unable to collect copper under these conditions. Copper uptake and gene expression, however, were not affected in wild-type M. trichosporium OB3b, indicating that the TonB-dependent transporter encoded by mbnT is responsible for methanobactin uptake and that methanobactin is a key mechanism used by methanotrophs for copper uptake. When the mbnT::Gmr mutant was grown under a range of copper concentrations in the absence of methanobactin, however, the phenotype of the mutant was indistinguishable from that of wild-type M. trichosporium OB3b, indicating that this methanotroph has multiple mechanisms for copper uptake.},
doi = {10.1128/AEM.03884-15},
journal = {Applied and Environmental Microbiology},
number = 6,
volume = 82,
place = {United States},
year = {2016},
month = {3}
}

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