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Title: Electroporation-Based Genetic Manipulation in Type I Methanotrophs

Abstract

Methane is becoming a major candidate for a prominent carbon feedstock in the future, and the bioconversion of methane into valuable products has drawn increasing attention. In order to facilitate the use of methanotrophic organisms as industrial strains and accelerate our ability to metabolically engineer methanotrophs, simple and rapid genetic tools are needed. Electroporation is one such enabling tool, but to date it has not been successful in a group of methanotrophs of interest for the production of chemicals and fuels, the gammaproteobacterial (type I) methanotrophs. In this study, we developed electroporation techniques with a high transformation efficiency for three different type I methanotrophs: Methylomicrobium buryatense 5GB1C, Methylomonas sp. strain LW13, and Methylobacter tundripaludum 21/22. We further developed this technique in M. buryatense, a haloalkaliphilic aerobic methanotroph that demonstrates robust growth with a high carbon conversion efficiency and is well suited for industrial use for the bioconversion of methane. On the basis of the high transformation efficiency of M. buryatense, gene knockouts or integration of a foreign fragment into the chromosome can be easily achieved by direct electroporation of PCR-generated deletion or integration constructs. Moreover, site-specific recombination (FLP-FRT [FLP recombination target] recombination) and sacB counterselection systems were employed to performmore » marker-free manipulation, and two new antibiotics, zeocin and hygromycin, were validated to be antibiotic markers in this strain. Together, these tools facilitate the rapid genetic manipulation of M. buryatense and other type I methanotrophs, promoting the ability to perform fundamental research and industrial process development with these strains.« less

Authors:
 [1];  [2];  [2];  [2];  [3]
  1. Univ. of Washington, Seattle, WA (United States). Dept. of Chemical Engineering; Nanjing Agricultural Univ. (China). College of Life Sciences and Dept. of Microbiology
  2. Univ. of Washington, Seattle, WA (United States). Dept. of Chemical Engineering
  3. Univ. of Washington, Seattle, WA (United States). Dept. of Chemical Engineering and Dept. of Microbiology
Publication Date:
Research Org.:
Univ. of Washington, Seattle, WA (United States)
Sponsoring Org.:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); National Basic Research Program of China; China Scholarship Council (CSC)
OSTI Identifier:
1470733
Grant/Contract Number:  
AR0000350; 2015CB150505; 201306855020
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 82; Journal Issue: 7; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Yan, Xin, Chu, Frances, Puri, Aaron W., Fu, Yanfen, and Lidstrom, Mary E. Electroporation-Based Genetic Manipulation in Type I Methanotrophs. United States: N. p., 2016. Web. doi:10.1128/AEM.03724-15.
Yan, Xin, Chu, Frances, Puri, Aaron W., Fu, Yanfen, & Lidstrom, Mary E. Electroporation-Based Genetic Manipulation in Type I Methanotrophs. United States. doi:10.1128/AEM.03724-15.
Yan, Xin, Chu, Frances, Puri, Aaron W., Fu, Yanfen, and Lidstrom, Mary E. Fri . "Electroporation-Based Genetic Manipulation in Type I Methanotrophs". United States. doi:10.1128/AEM.03724-15. https://www.osti.gov/servlets/purl/1470733.
@article{osti_1470733,
title = {Electroporation-Based Genetic Manipulation in Type I Methanotrophs},
author = {Yan, Xin and Chu, Frances and Puri, Aaron W. and Fu, Yanfen and Lidstrom, Mary E.},
abstractNote = {Methane is becoming a major candidate for a prominent carbon feedstock in the future, and the bioconversion of methane into valuable products has drawn increasing attention. In order to facilitate the use of methanotrophic organisms as industrial strains and accelerate our ability to metabolically engineer methanotrophs, simple and rapid genetic tools are needed. Electroporation is one such enabling tool, but to date it has not been successful in a group of methanotrophs of interest for the production of chemicals and fuels, the gammaproteobacterial (type I) methanotrophs. In this study, we developed electroporation techniques with a high transformation efficiency for three different type I methanotrophs: Methylomicrobium buryatense 5GB1C, Methylomonas sp. strain LW13, and Methylobacter tundripaludum 21/22. We further developed this technique in M. buryatense, a haloalkaliphilic aerobic methanotroph that demonstrates robust growth with a high carbon conversion efficiency and is well suited for industrial use for the bioconversion of methane. On the basis of the high transformation efficiency of M. buryatense, gene knockouts or integration of a foreign fragment into the chromosome can be easily achieved by direct electroporation of PCR-generated deletion or integration constructs. Moreover, site-specific recombination (FLP-FRT [FLP recombination target] recombination) and sacB counterselection systems were employed to perform marker-free manipulation, and two new antibiotics, zeocin and hygromycin, were validated to be antibiotic markers in this strain. Together, these tools facilitate the rapid genetic manipulation of M. buryatense and other type I methanotrophs, promoting the ability to perform fundamental research and industrial process development with these strains.},
doi = {10.1128/AEM.03724-15},
journal = {Applied and Environmental Microbiology},
number = 7,
volume = 82,
place = {United States},
year = {2016},
month = {1}
}

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