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Title: Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog

Abstract

The objective of this study was to characterize metabolically active, aerobic methanotrophs in an ombrotrophic peatland in the Marcell Experimental Forest, in Minnesota. Methanotrophs were investigated in the field and in laboratory incubations using DNA-stable isotope probing (SIP), expression studies on particulate methane monooxygenase ( pmoA) genes, and amplicon sequencing of 16S rRNA genes. Potential rates of oxidation ranged from 14 to 17 μmol of CH 4 g dry weight soil –1 day –1. Within DNA-SIP incubations, the relative abundance of methanotrophs increased from 4% in situ to 25 to 36% after 8 to 14 days. Phylogenetic analysis of the 13C-enriched DNA fractions revealed that the active methanotrophs were dominated by the genera Methylocystis (type II; Alphaproteobacteria), Methylomonas, and Methylovulum (both, type I; Gammaproteobacteria). In field samples, a transcript-to-gene ratio of 1 to 2 was observed for pmoA in surface peat layers, which attenuated rapidly with depth, indicating that the highest methane consumption was associated with a depth of 0 to 10 cm. Metagenomes and sequencing of cDNA pmoA amplicons from field samples confirmed that the dominant active methanotrophs were Methylocystis and Methylomonas. Although type II methanotrophs have long been shown to mediate methane consumption in peatlands, our results indicatemore » that members of the genera Methylomonas and Methylovulum (type I) can significantly contribute to aerobic methane oxidation in these ecosystems.« less

Authors:
 [1];  [1];  [2];  [3];  [4];  [1]
  1. Georgia Inst. of Technology, Atlanta, GA (United States). School of Biology
  2. Univ. of Western Australia, Perth, WA (Australia). School of Earth and Environment
  3. Florida State Univ., Tallahassee, FL (United States). Earth, Ocean, and Atmospheric Science
  4. Univ. of East Anglia, Norwich Research Park, Norwich (United Kingdom). School of Environmental Sciences
Publication Date:
Research Org.:
Georgia Inst. of Technology, Atlanta, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1470731
Grant/Contract Number:  
SC0007144
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 82; Journal Issue: 8; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Esson, Kaitlin C., Lin, Xueju, Kumaresan, Deepak, Chanton, Jeffrey P., Murrell, J. Colin, and Kostka, Joel E. Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog. United States: N. p., 2016. Web. doi:10.1128/AEM.03640-15.
Esson, Kaitlin C., Lin, Xueju, Kumaresan, Deepak, Chanton, Jeffrey P., Murrell, J. Colin, & Kostka, Joel E. Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog. United States. doi:10.1128/AEM.03640-15.
Esson, Kaitlin C., Lin, Xueju, Kumaresan, Deepak, Chanton, Jeffrey P., Murrell, J. Colin, and Kostka, Joel E. Fri . "Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog". United States. doi:10.1128/AEM.03640-15. https://www.osti.gov/servlets/purl/1470731.
@article{osti_1470731,
title = {Alpha- and Gammaproteobacterial Methanotrophs Codominate the Active Methane-Oxidizing Communities in an Acidic Boreal Peat Bog},
author = {Esson, Kaitlin C. and Lin, Xueju and Kumaresan, Deepak and Chanton, Jeffrey P. and Murrell, J. Colin and Kostka, Joel E.},
abstractNote = {The objective of this study was to characterize metabolically active, aerobic methanotrophs in an ombrotrophic peatland in the Marcell Experimental Forest, in Minnesota. Methanotrophs were investigated in the field and in laboratory incubations using DNA-stable isotope probing (SIP), expression studies on particulate methane monooxygenase (pmoA) genes, and amplicon sequencing of 16S rRNA genes. Potential rates of oxidation ranged from 14 to 17 μmol of CH4 g dry weight soil–1 day–1. Within DNA-SIP incubations, the relative abundance of methanotrophs increased from 4% in situ to 25 to 36% after 8 to 14 days. Phylogenetic analysis of the 13C-enriched DNA fractions revealed that the active methanotrophs were dominated by the genera Methylocystis (type II; Alphaproteobacteria), Methylomonas, and Methylovulum (both, type I; Gammaproteobacteria). In field samples, a transcript-to-gene ratio of 1 to 2 was observed for pmoA in surface peat layers, which attenuated rapidly with depth, indicating that the highest methane consumption was associated with a depth of 0 to 10 cm. Metagenomes and sequencing of cDNA pmoA amplicons from field samples confirmed that the dominant active methanotrophs were Methylocystis and Methylomonas. Although type II methanotrophs have long been shown to mediate methane consumption in peatlands, our results indicate that members of the genera Methylomonas and Methylovulum (type I) can significantly contribute to aerobic methane oxidation in these ecosystems.},
doi = {10.1128/AEM.03640-15},
journal = {Applied and Environmental Microbiology},
number = 8,
volume = 82,
place = {United States},
year = {2016},
month = {2}
}

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