Antimicrobial Properties of 2D MnO2 and MoS2 Nanomaterials Vertically Aligned on Graphene Materials and Ti3C2 MXene
Abstract
Two-dimensional (2D) nanomaterials have attracted considerable attention in biomedical and environmental applications due to their antimicrobial activity. In the interest of investigating the primary antimicrobial mode-of-action of 2D nanomaterials, we studied the antimicrobial properties of MnO2 and MoS2, toward Gram-positive and Gram-negative bacteria. Bacillus subtilis and Escherichia coli bacteria were treated individually with 100 μg/mL of randomly oriented and vertically aligned nanomaterials for ~3 h in the dark. The vertically aligned 2D MnO2 and MoS2 were grown on 2D sheets of graphene oxide, reduced graphene oxide, and Ti3C2 MXene. Measurements to determine the viability of bacteria in the presence of the 2D nanomaterials performed by using two complementary techniques, flow cytometry, and fluorescence imaging showed that, while MnO2 and MoS2 nanosheets show different antibacterial activities, in both cases, Gram-positive bacteria show a higher loss in membrane integrity. Scanning electron microscopy images suggest that the 2D nanomaterials, which have a detrimental effect on bacteria viability, compromise the cell wall, leading to significant morphological changes. Here, we propose that the peptidoglycan mesh (PM) in the bacterial wall is likely the primary target of the 2D nanomaterials. Vertically aligned 2D MnO2 nanosheets showed the highest antimicrobial activity, suggesting that the edges of themore »
- Authors:
-
[1];
[1];
[1];
[3];
[3];
[1]
- Temple Univ., Philadelphia, PA (United States). Dept. of Chemistry, and Center for Computational Design of Functional Layered Materials (CCDM)
- Temple Univ., Philadelphia, PA (United States). Dept. of Chemistry
- Drexel Univ., Philadelphia, PA (United States). Dept. of Materials Science and Engineering, and A.J. Drexel Nanomaterials Inst.
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for the Computational Design of Functional Layered Materials (CCDM)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1470630
- Grant/Contract Number:
- SC0012575
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Langmuir
- Additional Journal Information:
- Journal Volume: 34; Journal Issue: 24; Related Information: CCDM partners with Temple University (lead); Brookhaven National Laboratory; Drexel University; Duke University; North Carolina State University; Northeastern University; Princeton University; Rice University; University of Pennsylvania; Journal ID: ISSN 0743-7463
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; catalysis (heterogeneous); solar (photovoltaic); energy storage (including batteries and capacitors); hydrogen and fuel cells; defects; mechanical behavior; materials and chemistry by design; synthesis (novel materials); 2D nanomaterials; antimicrobial; birnessite; mode-of-action; nano-knife; cell wall; peptidoglycan mesh
Citation Formats
Alimohammadi, Farbod, Sharifian Gh., Mohammad, Attanayake, Nuwan H., Thenuwara, Akila C., Gogotsi, Yury, Anasori, Babak, and Strongin, Daniel R. Antimicrobial Properties of 2D MnO2 and MoS2 Nanomaterials Vertically Aligned on Graphene Materials and Ti3C2 MXene. United States: N. p., 2018.
Web. doi:10.1021/acs.langmuir.8b00262.
Alimohammadi, Farbod, Sharifian Gh., Mohammad, Attanayake, Nuwan H., Thenuwara, Akila C., Gogotsi, Yury, Anasori, Babak, & Strongin, Daniel R. Antimicrobial Properties of 2D MnO2 and MoS2 Nanomaterials Vertically Aligned on Graphene Materials and Ti3C2 MXene. United States. https://doi.org/10.1021/acs.langmuir.8b00262
Alimohammadi, Farbod, Sharifian Gh., Mohammad, Attanayake, Nuwan H., Thenuwara, Akila C., Gogotsi, Yury, Anasori, Babak, and Strongin, Daniel R. Mon .
"Antimicrobial Properties of 2D MnO2 and MoS2 Nanomaterials Vertically Aligned on Graphene Materials and Ti3C2 MXene". United States. https://doi.org/10.1021/acs.langmuir.8b00262. https://www.osti.gov/servlets/purl/1470630.
@article{osti_1470630,
title = {Antimicrobial Properties of 2D MnO2 and MoS2 Nanomaterials Vertically Aligned on Graphene Materials and Ti3C2 MXene},
author = {Alimohammadi, Farbod and Sharifian Gh., Mohammad and Attanayake, Nuwan H. and Thenuwara, Akila C. and Gogotsi, Yury and Anasori, Babak and Strongin, Daniel R.},
abstractNote = {Two-dimensional (2D) nanomaterials have attracted considerable attention in biomedical and environmental applications due to their antimicrobial activity. In the interest of investigating the primary antimicrobial mode-of-action of 2D nanomaterials, we studied the antimicrobial properties of MnO2 and MoS2, toward Gram-positive and Gram-negative bacteria. Bacillus subtilis and Escherichia coli bacteria were treated individually with 100 μg/mL of randomly oriented and vertically aligned nanomaterials for ~3 h in the dark. The vertically aligned 2D MnO2 and MoS2 were grown on 2D sheets of graphene oxide, reduced graphene oxide, and Ti3C2 MXene. Measurements to determine the viability of bacteria in the presence of the 2D nanomaterials performed by using two complementary techniques, flow cytometry, and fluorescence imaging showed that, while MnO2 and MoS2 nanosheets show different antibacterial activities, in both cases, Gram-positive bacteria show a higher loss in membrane integrity. Scanning electron microscopy images suggest that the 2D nanomaterials, which have a detrimental effect on bacteria viability, compromise the cell wall, leading to significant morphological changes. Here, we propose that the peptidoglycan mesh (PM) in the bacterial wall is likely the primary target of the 2D nanomaterials. Vertically aligned 2D MnO2 nanosheets showed the highest antimicrobial activity, suggesting that the edges of the nanosheets were likely compromising the cell walls upon contact.},
doi = {10.1021/acs.langmuir.8b00262},
journal = {Langmuir},
number = 24,
volume = 34,
place = {United States},
year = {2018},
month = {5}
}
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Works referenced in this record:
Bacterial resistance to silver in wound care
journal, May 2005
- Percival, S. L.; Bowler, P. G.; Russell, D.
- Journal of Hospital Infection, Vol. 60, Issue 1
Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds
journal, May 2006
- Silver, Simon; Phung, Le T.; Silver, Gregg
- Journal of Industrial Microbiology & Biotechnology, Vol. 33, Issue 7
Excess Mortality Associated with Antimicrobial Drug-Resistant Salmonella Typhimurium
journal, May 2002
- Helms, Morten; Vastrup, Pernille; Gerner-Smidt, Peter
- Emerging Infectious Diseases, Vol. 8, Issue 5
In situ synthesis of nano silver on cotton using Tollens’ reagent
journal, January 2012
- Montazer, Majid; Alimohammadi, Farbod; Shamei, Ali
- Carbohydrate Polymers, Vol. 87, Issue 2
A Novel Nanocomposite with Superior Antibacterial Activity: A Silver-Based Metal Organic Framework Embellished with Graphene Oxide
journal, April 2018
- Firouzjaei, Mostafa Dadashi; Shamsabadi, Ahmad Arabi; Sharifian Gh., Mohammad
- Advanced Materials Interfaces, Vol. 5, Issue 11
The controversial antibacterial activity of graphene-based materials
journal, August 2016
- Hegab, Hanaa M.; ElMekawy, Ahmed; Zou, Linda
- Carbon, Vol. 105
Antibacterial applications of graphene-based nanomaterials: Recent achievements and challenges
journal, October 2016
- Ji, Haiwei; Sun, Hanjun; Qu, Xiaogang
- Advanced Drug Delivery Reviews, Vol. 105
Graphene-Based Antibacterial Paper
journal, July 2010
- Hu, Wenbing; Peng, Cheng; Luo, Weijie
- ACS Nano, Vol. 4, Issue 7
Structural destabilization induced by lithium intercalation in MoS 2 and related compounds
journal, January 1983
- Py, M. A.; Haering, R. R.
- Canadian Journal of Physics, Vol. 61, Issue 1
Rapid water disinfection using vertically aligned MoS2 nanofilms and visible light
journal, August 2016
- Liu, Chong; Kong, Desheng; Hsu, Po-Chun
- Nature Nanotechnology, Vol. 11, Issue 12
Antibacterial Activities of Graphene Oxide–Molybdenum Disulfide Nanocomposite Films
journal, February 2017
- Kim, Tae In; Kwon, Buki; Yoon, Jonghee
- ACS Applied Materials & Interfaces, Vol. 9, Issue 9
Oxidative transformation of levofloxacin by δ-MnO2: Products, pathways and toxicity assessment
journal, January 2015
- Li, Yuan; Wei, Dongbin; Du, Yuguo
- Chemosphere, Vol. 119
Evaluation of Antimicrobial Activity of Green-Synthesized Manganese Oxide Nanoparticles and Comparative Studies with Curcuminaniline Functionalized Nanoform
journal, March 2017
- M., Muhamed Haneefa; M., Jayandran; V., Balasubramanian
- Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, Issue 3
Preferential killing of bacterial cells by hybrid carbon nanotube-MnO2 nanocomposite synthesized by novel microwave assisted processing
journal, December 2017
- Sivaraj, D.; Vijayalakshmi, K.
- Materials Science and Engineering: C, Vol. 81
Plant extract-mediated biogenic synthesis of silver, manganese dioxide, silver-doped manganese dioxide nanoparticles and their antibacterial activity against food- and water-borne pathogens
journal, February 2016
- Krishnaraj, Chandran; Ji, Byoung-Jun; Harper, Stacey L.
- Bioprocess and Biosystems Engineering, Vol. 39, Issue 5
Restricted uptake of ethidium bromide and propidium diiodide by denatured closed circular DNA in buoyant cesium chloride
journal, June 1974
- Grossman, Lawrence I.; Watson, Robert; Vinograd, Jerome
- Journal of Molecular Biology, Vol. 86, Issue 2
Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies
journal, June 1981
- Yeh, Chang-Jing G.; Hsi, Bae-Li; Page Faulk, W.
- Journal of Immunological Methods, Vol. 43, Issue 3
Fundamental aspects of the interaction of propidium diiodide with nuclei acids studied in a model system of polyacrylamide films.
journal, August 1981
- Tas, J.; Westerneng, G.
- Journal of Histochemistry & Cytochemistry, Vol. 29, Issue 8
The use of an Ethidium Analogue in the Dye-Buoyant Density Procedure for the Isolation of Closed Circular dna: the Variation of the Superhelix Density of Mitochondrial dna
journal, March 1969
- Hudson, B.; Upholt, W. B.; Devinny, J.
- Proceedings of the National Academy of Sciences, Vol. 62, Issue 3
Assessment ofE. coli andSalmonella viability and starvation by confocal laser microscopy and flow cytometry using rhodamine 123, DiBAC4(3), propidium iodide, and CTC
journal, December 1997
- L�pez-Amor�s, R.; Castel, S.; Comas-Riu, J.
- Cytometry, Vol. 29, Issue 4
Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol
journal, July 1995
- L�pez-Amor�s, R.; Comas, J.; Vives-Rego, J.
- Applied and Environmental Microbiology, Vol. 61, Issue 7
Development of a robust flow cytometric assay for determining numbers of viable bacteria
journal, July 1995
- Jepras, R. I.; Carter, J.; Pearson, S. C.
- Applied and Environmental Microbiology, Vol. 61, Issue 7
Escherichia coli survival in groundwater and effluent measured using a combination of propidium iodide and the green fluorescent protein
journal, July 2002
- Banning, N.; Toze, S.; Mee, B. J.
- Journal of Applied Microbiology, Vol. 93, Issue 1
Distinguishing between living and nonliving bacteria: Evaluation of the vital stain propidium iodide and its combined use with molecular probes in aquatic samples
journal, May 1998
- Williams, S. C.; Hong, Y.; Danavall, D. C. A.
- Journal of Microbiological Methods, Vol. 32, Issue 3
Rapid test for distinguishing membrane-active antibacterial agents
journal, October 2006
- Prakash Singh, Maya
- Journal of Microbiological Methods, Vol. 67, Issue 1
Assessing the Impact of Titanium Dioxide and Zinc Oxide Nanoparticles on Bacteria Using a Fluorescent-Based Cell Membrane Integrity Assay
journal, April 2010
- Mileyeva-Biebesheimer, Olga N.; Zaky, Amr; Gruden, Cyndee L.
- Environmental Engineering Science, Vol. 27, Issue 4
Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action
journal, July 2016
- Cushnie, T. P. Tim; O’Driscoll, Noëlle H.; Lamb, Andrew J.
- Cellular and Molecular Life Sciences, Vol. 73, Issue 23
Lateral Dimension-Dependent Antibacterial Activity of Graphene Oxide Sheets
journal, August 2012
- Liu, Shaobin; Hu, Ming; Zeng, Tingying Helen
- Langmuir, Vol. 28, Issue 33
The Gram Stain after More than a Century
journal, January 1996
- Popescu, Andreia; Doyle, R. J.
- Biotechnic & Histochemistry, Vol. 71, Issue 3
Atomic force microscopy study of the antibacterial effects of chitosans on Escherichia coli and Staphylococcus aureus
journal, September 2008
- Eaton, Peter; Fernandes, João C.; Pereira, Eulália
- Ultramicroscopy, Vol. 108, Issue 10
A comprehensive two-dimensional map of cytosolic proteins ofBacillus subtilis
journal, August 2001
- Büttner, Knut; Bernhardt, Jörg; Scharf, Christian
- ELECTROPHORESIS, Vol. 22, Issue 14
The Isoelectric Point of Bacterial Cells
journal, February 1953
- Harden, Virginia P.; Harris, John O.
- Journal of Bacteriology, Vol. 65, Issue 2
Effect of Percolation on the Capacitance of Supercapacitor Electrodes Prepared from Composites of Manganese Dioxide Nanoplatelets and Carbon Nanotubes
journal, August 2014
- Higgins, Thomas M.; McAteer, David; Coelho, João Carlos Mesquita
- ACS Nano, Vol. 8, Issue 9
Catalytic synergy effect of MoS 2 /reduced graphene oxide hybrids for a highly efficient hydrogen evolution reaction
journal, January 2017
- Lee, Jung Eun; Jung, Jaemin; Ko, Taeg Yeoung
- RSC Advances, Vol. 7, Issue 9
WSXM : A software for scanning probe microscopy and a tool for nanotechnology
journal, January 2007
- Horcas, I.; Fernández, R.; Gómez-Rodríguez, J. M.
- Review of Scientific Instruments, Vol. 78, Issue 1
Damage and Survival of Bacteria during Freeze-Drying and during Storage over a Ten-Year Period
journal, March 1963
- Harrison, A. P.; Pelczar, M. J.
- Journal of General Microbiology, Vol. 30, Issue 3
Works referencing / citing this record:
Applications of 2D MXenes in energy conversion and storage systems
journal, January 2019
- Pang, Jinbo; Mendes, Rafael G.; Bachmatiuk, Alicja
- Chemical Society Reviews, Vol. 48, Issue 1
Recent Progress in Two-Dimensional Antimicrobial Nanomaterials
journal, November 2018
- Miao, Hui; Teng, Zhenyuan; Wang, Chengyin
- Chemistry - A European Journal, Vol. 25, Issue 4
A manganese oxide (MnO x )-Based colorimetric nanosensor for indirect measurement of lipophilic and hydrophilic antioxidant capacity
journal, January 2020
- Can, Kader; Üzer, Ayşem; Apak, Reşat
- Analytical Methods, Vol. 12, Issue 4
Promoting Role of MXene Nanosheets in Biomedical Sciences: Therapeutic and Biosensing Innovations
journal, October 2018
- Soleymaniha, Mohammadreza; Shahbazi, Mohammad-Ali; Rafieerad, Ali Reza
- Advanced Healthcare Materials, Vol. 8, Issue 1
Influence of modification of Ti 3 C 2 MXene with ceramic oxide and noble metal nanoparticles on its antimicrobial properties and ecotoxicity towards selected algae and higher plants
journal, January 2019
- Rozmysłowska-Wojciechowska, A.; Karwowska, E.; Poźniak, S.
- RSC Advances, Vol. 9, Issue 8