Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer
Abstract
We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of $$\mu$$deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Finally, the spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.
- Authors:
-
- Purdue Univ., West Lafayette, IN (United States). Dept. of Physics and Astronomy
- Washington Univ., St. Louis, MO (United States). Dept. of Biology and Chemistry, Photosynthetic Antenna Research Center
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1470599
- Alternate Identifier(s):
- OSTI ID: 1425540
- Grant/Contract Number:
- SC0001035; FG02-09ER16084; SC0001341
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Review of Scientific Instruments
- Additional Journal Information:
- Journal Volume: 89; Journal Issue: 3; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 0034-6748
- Publisher:
- American Institute of Physics (AIP)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; solar (fuels); photosynthesis (natural and artificial); biofuels (including algae and biomass); bio-inspired; charge transport; membrane; synthesis (novel materials); synthesis (self-assembly)
Citation Formats
Stadnytskyi, Valentyn, Orf, Gregory S., Blankenship, Robert E., and Savikhin, Sergei. Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer. United States: N. p., 2018.
Web. doi:10.1063/1.5009468.
Stadnytskyi, Valentyn, Orf, Gregory S., Blankenship, Robert E., & Savikhin, Sergei. Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer. United States. https://doi.org/10.1063/1.5009468
Stadnytskyi, Valentyn, Orf, Gregory S., Blankenship, Robert E., and Savikhin, Sergei. Mon .
"Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer". United States. https://doi.org/10.1063/1.5009468. https://www.osti.gov/servlets/purl/1470599.
@article{osti_1470599,
title = {Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer},
author = {Stadnytskyi, Valentyn and Orf, Gregory S. and Blankenship, Robert E. and Savikhin, Sergei},
abstractNote = {We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of $\mu$deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Finally, the spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.},
doi = {10.1063/1.5009468},
journal = {Review of Scientific Instruments},
number = 3,
volume = 89,
place = {United States},
year = {Mon Mar 12 00:00:00 EDT 2018},
month = {Mon Mar 12 00:00:00 EDT 2018}
}
Web of Science
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