Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy

doi:10.1021/acs.jpcb.8b01537

Title: Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy

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Abstract

Sum frequency generation (SFG) vibrational spectroscopy can selectively detect and analyze noncentrosymmetric components interspersed in amorphous matrices; this principle has been used for studies of nanoscale structure and mesoscale assembly of cellulose in plant cell walls. However, the spectral information averaged over a large area or volume cannot provide regiospecific or tissue-specific information of different cells in plants. Here, we demonstrate spatially resolved SFG analysis and imaging by combining a broad-band SFG spectroscopy system with an optical microscope. The system was designed to irradiate both narrow-band 800 nm and broad-band tunable IR beams through a single reflective objective lens, but from opposite sides of the surface normal direction of the sample. The developed technique was used to reveal inhomogeneous distributions of cellulose microfibrils within single cell walls, such as cotton fibers and onion epidermis as well as among different tissues in Arabidopsis inflorescence stems and bamboo culms. SFG microscopy can be used for vibrational spectroscopic imaging of other biological systems in complement to conventional Fourier transform infrared spectroscopy and confocal Raman microscopy.

Authors:

Huang, Shixin ^[1]; Makarem, Mohamadamin ^[1]; Kiemle, Sarah N. ^[2]; Hamedi, Hossein ^[1]; Sau, Moujhuri ^[1]; Cosgrove, Daniel J. ^[2];

^[1]

Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering
Pennsylvania State Univ., University Park, PA (United States). Dept. of Biology

Publication Date:: 2018-04-26

Research Org.:: Energy Frontier Research Centers (EFRC), Washington D.C. (United States). Center for Lignocellulose Structure and Formation (CLSF)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

OSTI Identifier:: 1470553

Grant/Contract Number:: [SC0001090]

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry

Additional Journal Information:: [ Journal Volume: 122; Journal Issue: 19; Related Information: CLSF partners with Pennsylvania State University (lead); North Carolina State University; University of Rhode Island; Virginia Tech University]; Journal ID: ISSN 1520-6106

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; biofuels (including algae and biomass); bio-inspired; membrane; carbon sequestration; materials and chemistry by design; synthesis (self-assembly)

Citation Formats


                    Huang, Shixin, Makarem, Mohamadamin, Kiemle, Sarah N., Hamedi, Hossein, Sau, Moujhuri, Cosgrove, Daniel J., and Kim, Seong H. Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy.  United States: N. p., 2018. 
        Web.  doi:10.1021/acs.jpcb.8b01537.

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                    Huang, Shixin, Makarem, Mohamadamin, Kiemle, Sarah N., Hamedi, Hossein, Sau, Moujhuri, Cosgrove, Daniel J., & Kim, Seong H. Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy.  United States.  doi:10.1021/acs.jpcb.8b01537.

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                    Huang, Shixin, Makarem, Mohamadamin, Kiemle, Sarah N., Hamedi, Hossein, Sau, Moujhuri, Cosgrove, Daniel J., and Kim, Seong H. Thu .  
        "Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy".  United States.  doi:10.1021/acs.jpcb.8b01537.  https://www.osti.gov/servlets/purl/1470553.

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@article{osti_1470553,

  title        = {Inhomogeneity of Cellulose Microfibril Assembly in Plant Cell Walls Revealed with Sum Frequency Generation Microscopy},

  author       = {Huang, Shixin and Makarem, Mohamadamin and Kiemle, Sarah N. and Hamedi, Hossein and Sau, Moujhuri and Cosgrove, Daniel J. and Kim, Seong H.},

  abstractNote = {Sum frequency generation (SFG) vibrational spectroscopy can selectively detect and analyze noncentrosymmetric components interspersed in amorphous matrices; this principle has been used for studies of nanoscale structure and mesoscale assembly of cellulose in plant cell walls. However, the spectral information averaged over a large area or volume cannot provide regiospecific or tissue-specific information of different cells in plants. Here, we demonstrate spatially resolved SFG analysis and imaging by combining a broad-band SFG spectroscopy system with an optical microscope. The system was designed to irradiate both narrow-band 800 nm and broad-band tunable IR beams through a single reflective objective lens, but from opposite sides of the surface normal direction of the sample. The developed technique was used to reveal inhomogeneous distributions of cellulose microfibrils within single cell walls, such as cotton fibers and onion epidermis as well as among different tissues in Arabidopsis inflorescence stems and bamboo culms. SFG microscopy can be used for vibrational spectroscopic imaging of other biological systems in complement to conventional Fourier transform infrared spectroscopy and confocal Raman microscopy.},

  doi          = {10.1021/acs.jpcb.8b01537},

  journal      = {Journal of Physical Chemistry. B, Condensed Matter, Materials, Surfaces, Interfaces and Biophysical Chemistry},

  number       = [19],

  volume       = [122],

  place        = {United States},

  year         = {2018},

  month        = {4}

}

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DOI: 10.1021/acs.jpcb.8b01537

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