skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes

Abstract

Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBA d only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1];  [1]
  1. Univ. of Glasgow, Scotland (United Kingdom)
  2. Arizona State Univ., Tempe, AZ (United States)
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1470114
Grant/Contract Number:  
SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 135; Journal Issue: 1-3; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 0166-8595
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Photosynthesis; Purple bacteria; Puc genes; Light-harvesting; LH2

Citation Formats

Southall, June, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William, Carey, Anne-Marie, Kelly, Sharon M., de Percin Northumberland, Claire Ortmann, and Cogdell, Richard J. Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. United States: N. p., 2017. Web. doi:10.1007/s11120-017-0386-7.
Southall, June, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William, Carey, Anne-Marie, Kelly, Sharon M., de Percin Northumberland, Claire Ortmann, & Cogdell, Richard J. Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. United States. doi:10.1007/s11120-017-0386-7.
Southall, June, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William, Carey, Anne-Marie, Kelly, Sharon M., de Percin Northumberland, Claire Ortmann, and Cogdell, Richard J. Wed . "Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes". United States. doi:10.1007/s11120-017-0386-7. https://www.osti.gov/servlets/purl/1470114.
@article{osti_1470114,
title = {Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes},
author = {Southall, June and Henry, Sarah L. and Gardiner, Alastair T. and Roszak, Aleksander W. and Mullen, William and Carey, Anne-Marie and Kelly, Sharon M. and de Percin Northumberland, Claire Ortmann and Cogdell, Richard J.},
abstractNote = {Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBAd only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.},
doi = {10.1007/s11120-017-0386-7},
journal = {Photosynthesis Research},
number = 1-3,
volume = 135,
place = {United States},
year = {2017},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Figures / Tables:

Fig. 1 Fig. 1: PCR analysis of the five pucBA gene pairs from the Rps. palustris ΔpucBAabce quadruple mutant and the wild-type strain. The two outside lanes are M-Promega 1 kb ladder marker and lanes 1–10 contain PCR fragment sizes a follows from (1) wild-type pucBAa 1498 bp, (2) ΔpucBAa 1168 bp,more » (3) wild-type pucBAb 1047 bp, (4) ΔpucBAb 1661 bp, (5) wild-type pucBAc 1280 bp, (6) ΔpucBAc 930 bp, (7) wild-type pucBAd 1246 bp, (8) ΔpucBAd 1246, (9) wildtype pucBAe 1014 bp and (10) ΔpucBAe 1488. The different sizes of the respective wild-type and deleted pucBA PCR bands from all gene pairs except pucBAd, indicate that only this gene pair is present in genomic DNA. The primer sequences are listed in Supplementary Table 3. All bands were excised and confirmed by sequencing« less

Save / Share:

Works referenced in this record:

The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium
journal, October 2011

  • Brotosudarmo, Tatas H. P.; Collins, Aaron M.; Gall, Andrew
  • Biochemical Journal, Vol. 440, Issue 1
  • DOI: 10.1042/BJ20110575

Rhodobacter capsulatus puc operon: promoter location, transcript sizes and effects of deletions on photosynthetic growth
journal, January 1993


Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria
journal, April 1995

  • McDermott, G.; Prince, S. M.; Freer, A. A.
  • Nature, Vol. 374, Issue 6522
  • DOI: 10.1038/374517a0

Isolation and characterisation of the different B800–850 light-harvesting complexes from low- and high-light grown cells of Rhodopseudomonas palustris, strain 2.1.6
journal, March 1990

  • Evans, Mark B.; Hawthornthwaite, Anna M.; Cogdell, Richard J.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1016, Issue 1
  • DOI: 10.1016/0005-2728(90)90008-R

The structure and function of bacterial light-harvesting complexes (Review)
journal, January 2004


Overview of the CCP 4 suite and current developments
journal, March 2011

  • Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444910045749

xia2 : an expert system for macromolecular crystallography data reduction
journal, December 2009


The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes
journal, August 2006

  • Cogdell, Richard J.; Gall, Andrew; Köhler, Jürgen
  • Quarterly Reviews of Biophysics, Vol. 39, Issue 3
  • DOI: 10.1017/S0033583506004434

Characterisation of the LH2 spectral variants produced by the photosynthetic purple sulphur bacterium Allochromatium vinosum
journal, November 2014

  • Carey, Anne-Marie; Hacking, Kirsty; Picken, Nichola
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 11
  • DOI: 10.1016/j.bbabio.2014.07.022

The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila
journal, November 1993

  • Gardiner, Alastair T.; Cogdell, Richard J.; Takaichi, Shinichi
  • Photosynthesis Research, Vol. 38, Issue 2
  • DOI: 10.1007/BF00146415

REFMAC 5 for the refinement of macromolecular crystal structures
journal, March 2011

  • Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S0907444911001314

Chromatic Adaptation of Photosynthetic Membranes
journal, July 2005


Structure, function and organization of antenna polypeptides and antenna complexes from the three families of Rhodospirillaneae
journal, August 1992

  • Brunisholz, René A.; Zuber, Herbert
  • Journal of Photochemistry and Photobiology B: Biology, Vol. 15, Issue 1-2
  • DOI: 10.1016/1011-1344(92)87010-7

Absorption and Fluorescence Spectra of Light-Harvesting Bacteriochlorophyll-Protein Complexes from Rhodopseudomonas palustris in the Near-Infrared Region1
journal, January 1982


Light-harvesting II (B800-B850 complex) structural genes from Rhodopseudomonas capsulata
journal, January 1985

  • Youvan, D. C.; Ismail, S.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 1
  • DOI: 10.1073/pnas.82.1.58

An introduction to data reduction: space-group determination, scaling and intensity statistics
journal, March 2011

  • Evans, Philip R.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
  • DOI: 10.1107/S090744491003982X

Atomic Force Microscopy Studies of Native Photosynthetic Membranes
journal, May 2009

  • Sturgis, James N.; Tucker, Jaimey D.; Olsen, John D.
  • Biochemistry, Vol. 48, Issue 17
  • DOI: 10.1021/bi900045x

Molecular Origins and Consequences of High-800 LH2 in Roseobacter denitrificans
journal, August 2011

  • Duquesne, Katia; Blanchard, Cecile; Sturgis, James N.
  • Biochemistry, Vol. 50, Issue 31
  • DOI: 10.1021/bi200538j

Control of Synthesis of Reaction Center Bacteriochlorophyll in Photosynthetic Bacteria
journal, February 1972


DNA-directed spatial assembly of photosynthetic light-harvesting proteins
journal, January 2016

  • Henry, Sarah L.; Withers, Jamie M.; Singh, Ishwar
  • Organic & Biomolecular Chemistry, Vol. 14, Issue 4
  • DOI: 10.1039/c5ob02351c

How good are my data and what is the resolution?
journal, June 2013

  • Evans, Philip R.; Murshudov, Garib N.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
  • DOI: 10.1107/S0907444913000061

The native architecture of a photosynthetic membrane
journal, August 2004

  • Bahatyrova, Svetlana; Frese, Raoul N.; Siebert, C. Alistair
  • Nature, Vol. 430, Issue 7003
  • DOI: 10.1038/nature02823

Genetically modified photosynthetic antenna complexes with blueshifted absorbance bands
journal, February 1992

  • Fowler, G. J. S.; Visschers, R. W.; Grief, G. G.
  • Nature, Vol. 355, Issue 6363
  • DOI: 10.1038/355848a0

Cloning of a new antenna gene cluster and expression analysis of the antenna gene family of Rhodopseudomonas palustris
journal, November 1993


Three-dimensional Reconstruction of a Membrane-bending Complex
journal, March 2008

  • Qian, Pu; Bullough, Per A.; Hunter, C. Neil
  • Journal of Biological Chemistry, Vol. 283, Issue 20
  • DOI: 10.1074/jbc.M800625200

Analysis of the pigment content of an antenna pigment-protein complex from three strains of Rhodopseudomonas sphaeroides
journal, June 1978

  • Cogdell, Richard J.; Crofts, Antony R.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 502, Issue 3
  • DOI: 10.1016/0005-2728(78)90074-9

Rings, Ellipses and Horseshoes: How Purple Bacteria Harvest Solar Energy
journal, January 2004


Physiological and structural analysis of light-harvesting mutants of Rhodobacter sphaeroides.
journal, March 1988


Spectroscopic characterization of the low-light B800–850 light-harvesting complex of Rhodopseudomonas palustris, strain 2.1.6
journal, November 1992

  • van Mourik, Frank; Hawthornthwaite, Anna M.; Vonk, Carine
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1140, Issue 1
  • DOI: 10.1016/0005-2728(92)90023-U

Involvement of the C-Terminal Extension of the   Polypeptide and of the PucC Protein in LH2 Complex Biosynthesis in Rubrivivax gelatinosus
journal, April 2004


The 7.5-Å Electron Density and Spectroscopic Properties of a NovelLow-Light B800 LH2 from Rhodopseudomonas palustris
journal, February 2002


XDS
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
  • DOI: 10.1107/S0907444909047337

The crystal structure of the light-harvesting complex II (B800–850) from Rhodospirillum molischianum
journal, May 1996


Complete genome sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas palustris
journal, December 2003

  • Larimer, Frank W; Chain, Patrick; Hauser, Loren
  • Nature Biotechnology, Vol. 22, Issue 1, p. 55-61
  • DOI: 10.1038/nbt923

Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
journal, January 2010

  • Caffrey, Martin; Porter, Christopher
  • Journal of Visualized Experiments, Issue 45
  • DOI: 10.3791/1712

Spectroscopic Analysis of Bacteriochlorophylls in Vitro and in vivo
journal, August 1966