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Title: Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment

Abstract

The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. Strain UFO1 was isolated under strictly anaerobic conditions from an enrichment established with pristine sediment from the Department of Energy Oak Ridge Field Research Center (ORFRC), Tennessee. Cultures were provided with acetate and 2-line ferrihydrite. Strain UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate. Ferrihydrite was not directly reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 hours when UFO1 was provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 hours. Strain UFO1 appears to be a novel species within the recently described bacterial genus Pelosinus, having 98.16 % 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol %, and DNA-DNA hybridization ofmore » strain UFO1 against Pelosinus fermentans R7T indicated an average 16.8% DNA-DNA similarity. The reduction of AQDS, Fe(III), and Cr(VI) suggests a potentially important role for strain UFO1 and similar organisms in influencing the biogeochemistry of pristine and contaminated geologic media at the ORFRC. The unique phylogenetic and metal-transforming characteristics of strain UFO1 reveal it is a novel species of a recently-described genus; therefore, we propose a new species, Pelosinus lilae sp. nov.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [4];  [6]
+ Show Author Affiliations
  1. Idaho National Lab. (INL), Idaho Falls, ID (United States); Idaho State Univ., Pocatello, ID (United States)
  2. Idaho State Univ., Pocatello, ID (United States); California Inst. of Technology (CalTech), Pasadena, CA (United States)
  3. Univ. of Georgia, Aiken, SC (United States). Savannah River Ecology Lab.
  4. Idaho National Lab. (INL), Idaho Falls, ID (United States)
  5. Point Loma Nazarene Univ., San Diego, CA (United States)
  6. Idaho State Univ., Pocatello, ID (United States)
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE Office of Nuclear Energy (NE)
OSTI Identifier:
1469645
Report Number(s):
[INL/JOU-08-14091-Rev000]
[Journal ID: ISSN 1664-302X]
Grant/Contract Number:  
[AC07-05ID14517]
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
[ Journal Volume: 9; Journal Issue: -]; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; metals bioremediation; metal-reducing bacteria; iron-reducing bacteria

Citation Formats

Ray, Allison E., Connon, Stephanie A., Neal, Andrew L., Fujita, Yoshiko, Cummings, David E., Ingram, Jani C., and Magnuson, Timothy S. Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.01689.
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Ray, Allison E., Connon, Stephanie A., Neal, Andrew L., Fujita, Yoshiko, Cummings, David E., Ingram, Jani C., & Magnuson, Timothy S. Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment. United States. doi:10.3389/fmicb.2018.01689.
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Ray, Allison E., Connon, Stephanie A., Neal, Andrew L., Fujita, Yoshiko, Cummings, David E., Ingram, Jani C., and Magnuson, Timothy S. Fri . "Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment". United States. doi:10.3389/fmicb.2018.01689. https://www.osti.gov/servlets/purl/1469645.
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@article{osti_1469645,
title = {Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment},
author = {Ray, Allison E. and Connon, Stephanie A. and Neal, Andrew L. and Fujita, Yoshiko and Cummings, David E. and Ingram, Jani C. and Magnuson, Timothy S.},
abstractNote = {The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. Strain UFO1 was isolated under strictly anaerobic conditions from an enrichment established with pristine sediment from the Department of Energy Oak Ridge Field Research Center (ORFRC), Tennessee. Cultures were provided with acetate and 2-line ferrihydrite. Strain UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate. Ferrihydrite was not directly reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 hours when UFO1 was provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 hours. Strain UFO1 appears to be a novel species within the recently described bacterial genus Pelosinus, having 98.16 % 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol %, and DNA-DNA hybridization of strain UFO1 against Pelosinus fermentans R7T indicated an average 16.8% DNA-DNA similarity. The reduction of AQDS, Fe(III), and Cr(VI) suggests a potentially important role for strain UFO1 and similar organisms in influencing the biogeochemistry of pristine and contaminated geologic media at the ORFRC. The unique phylogenetic and metal-transforming characteristics of strain UFO1 reveal it is a novel species of a recently-described genus; therefore, we propose a new species, Pelosinus lilae sp. nov.},
doi = {10.3389/fmicb.2018.01689},
journal = {Frontiers in Microbiology},
number = [-],
volume = [9],
place = {United States},
year = {2018},
month = {8}
}
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DOI:  10.3389/fmicb.2018.01689
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Works referenced in this record:

Calculation of multiplet structure of core p -vacancy levels. II
journal, July 1975

Enumeration and Characterization of Iron(III)-Reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium(VI)
journal, December 2003

Effects of Humic Substances and Quinones at Low Concentrations on Ferrihydrite Reduction by Geobacter metallireducens
journal, August 2009

  • Wolf, Manfred; Kappler, Andreas; Jiang, Jie
  • Environmental Science & Technology, Vol. 43, Issue 15
  • DOI: 10.1021/es803647r

Phylogenetic Analysis of Aquaspirillum magnetotacticum Using Polymerase Chain Reaction-Amplified 16S rRNA-Specific DNA
journal, April 1991

  • Eden, P. A.; Schmidt, T. M.; Blakemore, R. P.
  • International Journal of Systematic Bacteriology, Vol. 41, Issue 2
  • DOI: 10.1099/00207713-41-2-324

Microbes with a mettle for bioremediation
journal, June 2000

  • Lovley, Derek R.; Lloyd, Jon R.
  • Nature Biotechnology, Vol. 18, Issue 6
  • DOI: 10.1038/76433

Anaerobic production of magnetite by a dissimilatory iron-reducing microorganism
journal, November 1987

  • Lovley, Derek R.; Stolz, John F.; Nord, Gordon L.
  • Nature, Vol. 330, Issue 6145
  • DOI: 10.1038/330252a0

Impact of Ferrihydrite and Anthraquinone-2,6-Disulfonate on the Reductive Transformation of 2,4,6-Trinitrotoluene by a Gram-Positive Fermenting Bacterium
journal, September 2005

  • Borch, Thomas; Inskeep, William P.; Harwood, Jace A.
  • Environmental Science & Technology, Vol. 39, Issue 18
  • DOI: 10.1021/es0504441

Quinone Moieties Act as Electron Acceptors in the Reduction of Humic Substances by Humics-Reducing Microorganisms
journal, October 1998

  • Scott, Durelle T.; McKnight, Diane M.; Blunt-Harris, Elizabeth L.
  • Environmental Science & Technology, Vol. 32, Issue 19
  • DOI: 10.1021/es980272q

X-ray photoelectron and Auger electron spectroscopic studies of pyrrhotite and mechanism of air oxidation
journal, January 1994

Anaerovibrio glycerini sp. nov., an anaerobic bacterium fermenting glycerol to propionate, cell matter, and hydrogen
journal, October 1989

  • Schauder, Rolf; Schink, Bernhard
  • Archives of Microbiology, Vol. 152, Issue 5
  • DOI: 10.1007/BF00446932

LPSN—list of prokaryotic names with standing in nomenclature
journal, November 2013

Clostridium quercicolum sp.n., isolated from discolored tissues in living oak trees
journal, December 1971

  • Stankewich, J. P.; Cosenza, B. J.; Shigo, A. L.
  • Antonie van Leeuwenhoek, Vol. 37, Issue 1
  • DOI: 10.1007/BF02218500

Reclassification of Clostridium quercicolum as Dendrosporobacter quercicolus gen. nov., comb. nov
journal, January 2000

  • Strompl, C.; Tindall, B. J.; Lunsdorf, H.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 50, Issue 1
  • DOI: 10.1099/00207713-50-1-101

Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses
journal, January 2007

  • Shelobolina, E. S.; Nevin, K. P.; Blakeney-Hayward, J. D.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 57, Issue 1
  • DOI: 10.1099/ijs.0.64221-0

Microbial solubilization and immobilization of toxic metals: key biogeochemical processes for treatment of contamination
journal, July 1997

Sleuthing out bacterial identities
journal, May 1989

Electron shuttling via humic acids in microbial iron(III) reduction in a freshwater sediment
journal, January 2004

Humic substances as electron acceptors for microbial respiration
journal, August 1996

  • Lovley, Derek R.; Coates, John D.; Blunt-Harris, Elizabeth L.
  • Nature, Vol. 382, Issue 6590
  • DOI: 10.1038/382445a0

Biosorption and bioaccumulation of heavy metals on dead and living biomass of Bacillus sphaericus
journal, August 2009

Evidence for multiple modes of uranium immobilization by an anaerobic bacterium
journal, May 2011

  • Ray, Allison E.; Bargar, John R.; Sivaswamy, Vaideeswaran
  • Geochimica et Cosmochimica Acta, Vol. 75, Issue 10
  • DOI: 10.1016/j.gca.2011.02.040

Potential for Nonenzymatic Reduction of Fe(III) via Electron Shuttling in Subsurface Sediments
journal, June 2000

  • Nevin, Kelly P.; Lovley, Derek R.
  • Environmental Science & Technology, Vol. 34, Issue 12
  • DOI: 10.1021/es991181b

In situ measurement of Fe(III) reduction activity of Geobacter pelophilus by simultaneous in situ RT-PCR and XPS analysis
journal, July 2004

Genomic and Physiological Characterization of the Chromate-Reducing, Aquifer-Derived Firmicute Pelosinus sp. Strain HCF1
journal, October 2012

  • Beller, Harry R.; Han, Ruyang; Karaoz, Ulas
  • Applied and Environmental Microbiology, Vol. 79, Issue 1
  • DOI: 10.1128/AEM.02496-12

A rapid method for the base ratio determination of bacterial DNA
journal, August 1977

Studies on the spectrophotometric determination of DNA hybridization from renaturation rates
journal, April 1983

  • Huss, Volker A. R.; Festl, Herbert; Schleifer, Karl Heinz
  • Systematic and Applied Microbiology, Vol. 4, Issue 2
  • DOI: 10.1016/S0723-2020(83)80048-4

Evolutionary and Diagnostic Implications of Intragenomic Heterogeneity in the 16S rRNA Gene in Aeromonas Strains
journal, September 2005

Complete Genome Sequence of Pelosinus sp. Strain UFO1 Assembled Using Single-Molecule Real-Time DNA Sequencing Technology
journal, September 2014

Acetonema longum gen.nov.sp.nov., an H2/CO2 acetogenic bacterium from the termite, Pterotermes occidentis
journal, August 1991

  • Kane, Matthew D.; Breznak, John A.
  • Archives of Microbiology, Vol. 156, Issue 2
  • DOI: 10.1007/BF00290979

Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
journal, October 1985

  • Lane, D. J.; Pace, B.; Olsen, G. J.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 20
  • DOI: 10.1073/pnas.82.20.6955

The Quantitative Measurement of DNA Hybridization from Renaturation Rates
journal, January 1970

The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012

  • Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1219

Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics
journal, October 1987

  • Moore, W. E. C.; Stackebrandt, E.; Kandler, O.
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 37, Issue 4
  • DOI: 10.1099/00207713-37-4-463

Pelosinus defluvii sp. nov., isolated from chlorinated solvent-contaminated groundwater, emended description of the genus Pelosinus and transfer of Sporotalea propionica to Pelosinus propionicus comb. nov.
journal, August 2011

  • Moe, W. M.; Stebbing, R. E.; Rao, J. U.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 6
  • DOI: 10.1099/ijs.0.033753-0

Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography
journal, April 1989

  • Mesbah, M.; Premachandran, U.; Whitman, W. B.
  • International Journal of Systematic Bacteriology, Vol. 39, Issue 2
  • DOI: 10.1099/00207713-39-2-159

BIOREMEDIATION: Anaerobes to the Rescue
journal, August 2001

Iron sulfides and sulfur species produced at hematite surfaces in the presence of sulfate-reducing bacteria
journal, January 2001

  • Neal, Andrew L.; Techkarnjanaruk, Somkiet; Dohnalkova, Alice
  • Geochimica et Cosmochimica Acta, Vol. 65, Issue 2
  • DOI: 10.1016/S0016-7037(00)00537-8

Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis.
journal, October 1996

Arsenic Mobilization by the Dissimilatory Fe(III)-Reducing Bacterium Shewanella alga BrY
journal, March 1999

  • Cummings, David E.; Caccavo,, Frank; Fendorf, Scott
  • Environmental Science & Technology, Vol. 33, Issue 5
  • DOI: 10.1021/es980541c

Comparative Distribution and Taxonomic Value of Cellular Fatty Acids in Thirty-Three Genera of Anaerobic Gram-Negative Bacilli
journal, April 1994

  • Moore, L. V. H.; Bourne, D. M.; Moore, W. E. C.
  • International Journal of Systematic Bacteriology, Vol. 44, Issue 2
  • DOI: 10.1099/00207713-44-2-338

X-ray photoelectron spectroscopic studies of iron oxides
journal, September 1977

  • McIntyre, N. S.; Zetaruk, D. G.
  • Analytical Chemistry, Vol. 49, Issue 11
  • DOI: 10.1021/ac50019a016

High-Resolution X-Ray Photoemission Spectrum of the Valence Bands of Gold
journal, June 1972

Microbial reduction of metals and radionuclides
journal, June 2003

A Highly Expressed High-Molecular-Weight S-Layer Complex of Pelosinus sp. Strain UFO1 Binds Uranium
journal, December 2016

  • Thorgersen, Michael P.; Lancaster, W. Andrew; Rajeev, Lara
  • Applied and Environmental Microbiology, Vol. 83, Issue 4
  • DOI: 10.1128/AEM.03044-16

Dating of the human-ape splitting by a molecular clock of mitochondrial DNA
journal, October 1985

  • Hasegawa, Masami; Kishino, Hirohisa; Yano, Taka-aki
  • Journal of Molecular Evolution, Vol. 22, Issue 2
  • DOI: 10.1007/BF02101694

Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui
journal, December 2000

  • Amann, Gudrun; Stetter, K. O.; Llobet-Brossa, Enric
  • Extremophiles, Vol. 4, Issue 6
  • DOI: 10.1007/s007920070007

Biostimulation by Glycerol Phosphate to Precipitate Recalcitrant Uranium(IV) Phosphate
journal, July 2015

  • Newsome, Laura; Morris, Katherine; Trivedi, Divyesh
  • Environmental Science & Technology, Vol. 49, Issue 18
  • DOI: 10.1021/acs.est.5b02042

Reduction of Fe(III), Cr(VI), U(VI), and Tc(VII) by Deinococcus radiodurans R1
journal, May 2000

Mediator compounds for the electrochemical study of biological redox systems: a compilation
journal, August 1982

Humic Substances as a Mediator for Microbially Catalyzed Metal Reduction
journal, May 1998

Surface chemistry and morphology of poorly crystalline iron sulfides precipitated in media containing sulfate-reducing bacteria
journal, January 1998

Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp
journal, April 2007

A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood
journal, October 2003

Intragenomic heterogeneity of the 16S rRNA gene in strain UFO1 caused by a 100-bp insertion in helix 6: Intragenomic heterogeneity in helix 6 of UFO1 16S rRNA gene
journal, March 2010

Adventitious carbon?the panacea for energy referencing?
journal, April 1982

Sulfate Requirement for the Growth of U(VI)-Reducing Bacteria in an Ethanol-Fed Enrichment
journal, February 2007

Dissimilatory reduction of Cr(VI), Fe(III), and U(VI) by Cellulomonas isolates
journal, October 2002

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