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Title: Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment

Abstract

The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. Strain UFO1 was isolated under strictly anaerobic conditions from an enrichment established with pristine sediment from the Department of Energy Oak Ridge Field Research Center (ORFRC), Tennessee. Cultures were provided with acetate and 2-line ferrihydrite. Strain UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate. Ferrihydrite was not directly reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 hours when UFO1 was provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 hours. Strain UFO1 appears to be a novel species within the recently described bacterial genus Pelosinus, having 98.16 % 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol %, and DNA-DNA hybridization ofmore » strain UFO1 against Pelosinus fermentans R7T indicated an average 16.8% DNA-DNA similarity. The reduction of AQDS, Fe(III), and Cr(VI) suggests a potentially important role for strain UFO1 and similar organisms in influencing the biogeochemistry of pristine and contaminated geologic media at the ORFRC. The unique phylogenetic and metal-transforming characteristics of strain UFO1 reveal it is a novel species of a recently-described genus; therefore, we propose a new species, Pelosinus lilae sp. nov.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [4];  [6]
  1. Idaho National Lab. (INL), Idaho Falls, ID (United States); Idaho State Univ., Pocatello, ID (United States)
  2. Idaho State Univ., Pocatello, ID (United States); California Inst. of Technology (CalTech), Pasadena, CA (United States)
  3. Univ. of Georgia, Aiken, SC (United States). Savannah River Ecology Lab.
  4. Idaho National Lab. (INL), Idaho Falls, ID (United States)
  5. Point Loma Nazarene Univ., San Diego, CA (United States)
  6. Idaho State Univ., Pocatello, ID (United States)
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE Office of Nuclear Energy (NE)
OSTI Identifier:
1469645
Report Number(s):
[INL/JOU-08-14091-Rev000]
[Journal ID: ISSN 1664-302X]
Grant/Contract Number:  
[AC07-05ID14517]
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
[ Journal Volume: 9; Journal Issue: -]; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; metals bioremediation; metal-reducing bacteria; iron-reducing bacteria

Citation Formats

Ray, Allison E., Connon, Stephanie A., Neal, Andrew L., Fujita, Yoshiko, Cummings, David E., Ingram, Jani C., and Magnuson, Timothy S. Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment. United States: N. p., 2018. Web. doi:10.3389/fmicb.2018.01689.
Ray, Allison E., Connon, Stephanie A., Neal, Andrew L., Fujita, Yoshiko, Cummings, David E., Ingram, Jani C., & Magnuson, Timothy S. Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment. United States. doi:10.3389/fmicb.2018.01689.
Ray, Allison E., Connon, Stephanie A., Neal, Andrew L., Fujita, Yoshiko, Cummings, David E., Ingram, Jani C., and Magnuson, Timothy S. Fri . "Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment". United States. doi:10.3389/fmicb.2018.01689. https://www.osti.gov/servlets/purl/1469645.
@article{osti_1469645,
title = {Metal Transformation by a Novel Pelosinus Isolate From a Subsurface Environment},
author = {Ray, Allison E. and Connon, Stephanie A. and Neal, Andrew L. and Fujita, Yoshiko and Cummings, David E. and Ingram, Jani C. and Magnuson, Timothy S.},
abstractNote = {The capability of microorganisms to alter metal speciation offers potential for the development of new strategies for immobilization of toxic metals in the environment. Strain UFO1 was isolated under strictly anaerobic conditions from an enrichment established with pristine sediment from the Department of Energy Oak Ridge Field Research Center (ORFRC), Tennessee. Cultures were provided with acetate and 2-line ferrihydrite. Strain UFO1 is a rod-shaped, spore-forming, and Gram-variable anaerobe with a fermentative metabolism. It is capable of reducing the humic acid analog anthraquinone-2,6-disulfonate (AQDS) using a variety of fermentable substrates and H2. Reduction of Fe(III)-nitrilotriacetic acid occurred in the presence of lactate. Ferrihydrite was not directly reduced in the absence of AQDS. Nearly complete reduction of 1, 3, and 5 ppm Cr(VI) occurred within 24 hours when UFO1 was provided with 10 mM lactate; when 1 mM AQDS was added, 3 and 5 ppm Cr(VI) were reduced to 0.1 ppm within 2 hours. Strain UFO1 appears to be a novel species within the recently described bacterial genus Pelosinus, having 98.16 % 16S rRNA gene sequence similarity with the most closely related described species, Pelosinus fermentans R7T. The G+C content of the genomic DNA was 38 mol %, and DNA-DNA hybridization of strain UFO1 against Pelosinus fermentans R7T indicated an average 16.8% DNA-DNA similarity. The reduction of AQDS, Fe(III), and Cr(VI) suggests a potentially important role for strain UFO1 and similar organisms in influencing the biogeochemistry of pristine and contaminated geologic media at the ORFRC. The unique phylogenetic and metal-transforming characteristics of strain UFO1 reveal it is a novel species of a recently-described genus; therefore, we propose a new species, Pelosinus lilae sp. nov.},
doi = {10.3389/fmicb.2018.01689},
journal = {Frontiers in Microbiology},
number = [-],
volume = [9],
place = {United States},
year = {2018},
month = {8}
}

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