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Title: IR-Live: fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells

Abstract

Water is a strong mid-infrared absorber, which has hindered the full exploitation of label-free and non-invasive infrared (IR) spectromicroscopy techniques for the study of living biological samples. To overcome this barrier, many researchers have built sophisticated fluidic chambers or microfluidic chips wherein the depth of the liquid medium in the sample compartment is limited to 10 μm or less. We report an innovative and simple way to fabricate plastic devices with infrared transparent view-ports enabling infrared spectromicroscopy of living biological samples; therefore the device is named “IR-Live”. Advantages of this approach include lower production costs, a minimal need to access a micro-fabrication facility, and unlimited mass or waste exchange for the living samples surrounding the view-port area. We demonstrate that the low-cost IR-Live in combination with microfluidic perfusion techniques enables long term (>60 h) cell culture, which broadens the capability of IR spectromicroscopy for studying living biological samples. To illustrate this, we first applied the device to study protein and lipid polarity in migrating REF52 fibroblasts by collecting 2-dimensional spectral chemical maps at a micrometer spatial resolution. Then, we demonstrated the suitability of our approach to study dynamic cellular events by collecting a time series of spectral maps of U937more » monocytes during the early stage of cell attachment to a bio-compatible surface.« less

Authors:
 [1];  [2];  [2];  [2];  [3]; ORCiD logo [2]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Berkeley Synchrotron Infrared Structural Biology (BSISB) Imaging Project; Elettra–Sincrotrone Trieste, Trieste (Italy)
  2. National Univ. of Singapore (Singapore). Mechanobiology Inst. (MBI)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Berkeley Synchrotron Infrared Structural Biology (BSISB) Imaging Project
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1469125
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Lab on a chip (Print)
Additional Journal Information:
Journal Name: Lab on a chip (Print); Journal Volume: 16; Journal Issue: 9; Related Information: © 2016 The Royal Society of Chemistry.; Journal ID: ISSN 1473-0197
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Birarda, G., Ravasio, A., Suryana, M., Maniam, S., Holman, H. -Y. N., and Grenci, G. IR-Live: fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells. United States: N. p., 2016. Web. doi:10.1039/c5lc01460c.
Birarda, G., Ravasio, A., Suryana, M., Maniam, S., Holman, H. -Y. N., & Grenci, G. IR-Live: fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells. United States. https://doi.org/10.1039/c5lc01460c
Birarda, G., Ravasio, A., Suryana, M., Maniam, S., Holman, H. -Y. N., and Grenci, G. Tue . "IR-Live: fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells". United States. https://doi.org/10.1039/c5lc01460c. https://www.osti.gov/servlets/purl/1469125.
@article{osti_1469125,
title = {IR-Live: fabrication of a low-cost plastic microfluidic device for infrared spectromicroscopy of living cells},
author = {Birarda, G. and Ravasio, A. and Suryana, M. and Maniam, S. and Holman, H. -Y. N. and Grenci, G.},
abstractNote = {Water is a strong mid-infrared absorber, which has hindered the full exploitation of label-free and non-invasive infrared (IR) spectromicroscopy techniques for the study of living biological samples. To overcome this barrier, many researchers have built sophisticated fluidic chambers or microfluidic chips wherein the depth of the liquid medium in the sample compartment is limited to 10 μm or less. We report an innovative and simple way to fabricate plastic devices with infrared transparent view-ports enabling infrared spectromicroscopy of living biological samples; therefore the device is named “IR-Live”. Advantages of this approach include lower production costs, a minimal need to access a micro-fabrication facility, and unlimited mass or waste exchange for the living samples surrounding the view-port area. We demonstrate that the low-cost IR-Live in combination with microfluidic perfusion techniques enables long term (>60 h) cell culture, which broadens the capability of IR spectromicroscopy for studying living biological samples. To illustrate this, we first applied the device to study protein and lipid polarity in migrating REF52 fibroblasts by collecting 2-dimensional spectral chemical maps at a micrometer spatial resolution. Then, we demonstrated the suitability of our approach to study dynamic cellular events by collecting a time series of spectral maps of U937 monocytes during the early stage of cell attachment to a bio-compatible surface.},
doi = {10.1039/c5lc01460c},
journal = {Lab on a chip (Print)},
number = 9,
volume = 16,
place = {United States},
year = {Tue Mar 15 00:00:00 EDT 2016},
month = {Tue Mar 15 00:00:00 EDT 2016}
}

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Works referenced in this record:

Infrared Spectroscopy in Aqueous Solution: Difficulties and Accuracy of Water Subtraction
journal, February 1997


SU-8 bonding protocol for the fabrication of microfluidic devices dedicated to FTIR microspectroscopy of live cells
journal, January 2014

  • Mitri, Elisa; Birarda, Giovanni; Vaccari, Lisa
  • Lab Chip, Vol. 14, Issue 1
  • DOI: 10.1039/C3LC50878A

Profiling pluripotent stem cells and organelles using synchrotron radiation infrared microspectroscopy
journal, November 2012

  • Sandt, Christophe; Frederick, Joni; Dumas, Paul
  • Journal of Biophotonics, Vol. 6, Issue 1
  • DOI: 10.1002/jbio.201200139

Tomographic phase microscopy
journal, August 2007

  • Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran
  • Nature Methods, Vol. 4, Issue 9
  • DOI: 10.1038/nmeth1078

Infrared microspectroscopy of live cells in aqueous media
journal, January 2010

  • (Swain) Marcsisin, Ellen J.; Uttero, Christina M.; Miljković, Miloš
  • The Analyst, Vol. 135, Issue 12
  • DOI: 10.1039/c0an00548g

FTIR spectroscopic imaging of protein aggregation in living cells
journal, October 2013

  • Miller, Lisa M.; Bourassa, Megan W.; Smith, Randy J.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1828, Issue 10
  • DOI: 10.1016/j.bbamem.2013.01.014

Infrared Microspectroscopy of Live Cells in Microfluidic Devices (MD-IRMS): Toward a Powerful Label-Free Cell-Based Assay
journal, June 2012

  • Vaccari, L.; Birarda, G.; Businaro, L.
  • Analytical Chemistry, Vol. 84, Issue 11
  • DOI: 10.1021/ac300313x

Synchrotron-based infrared spectroscopy brings to light the structure of protein aggregates in neurodegenerative diseases
journal, January 2014

  • Hoffner, Guylaine; André, William; Sandt, Christophe
  • Reviews in Analytical Chemistry, Vol. 33, Issue 4
  • DOI: 10.1515/revac-2014-0016

Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function
journal, February 2009

  • Pavani, S. R. P.; Thompson, M. A.; Biteen, J. S.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 9
  • DOI: 10.1073/pnas.0900245106

Infrared microspectroscopy of biochemical response of living cells in microfabricated devices
journal, May 2010


Resolution limits for infrared microspectroscopy explored with synchrotron radiation
journal, January 2001

  • Carr, G. L.
  • Review of Scientific Instruments, Vol. 72, Issue 3
  • DOI: 10.1063/1.1347965

The effect of optical substrates on micro-FTIR analysis of single mammalian cells
journal, November 2012

  • Wehbe, Katia; Filik, Jacob; Frogley, Mark D.
  • Analytical and Bioanalytical Chemistry, Vol. 405, Issue 4
  • DOI: 10.1007/s00216-012-6521-6

Synchrotron IR Spectromicroscopy: Chemistry of Living Cells
journal, November 2010

  • Holman, Hoi-Ying N.; Bechtel, Hans A.; Hao, Zhao
  • Analytical Chemistry, Vol. 82, Issue 21
  • DOI: 10.1021/ac100991d

High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams
journal, March 2011

  • Nasse, Michael J.; Walsh, Michael J.; Mattson, Eric C.
  • Nature Methods, Vol. 8, Issue 5
  • DOI: 10.1038/nmeth.1585

Rapid prototyping polymers for microfluidic devices and high pressure injections
journal, January 2011

  • Sollier, Elodie; Murray, Coleman; Maoddi, Pietro
  • Lab on a Chip, Vol. 11, Issue 22
  • DOI: 10.1039/c1lc20514e

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
journal, January 1994


Synchrotron Infrared Measurements of Protein Phosphorylation in Living Single PC12 Cells during Neuronal Differentiation
journal, April 2012

  • Chen, Liang; Holman, Hoi-Ying N.; Hao, Zhao
  • Analytical Chemistry, Vol. 84, Issue 9
  • DOI: 10.1021/ac300308x

Dynamic Full-Field Infrared Imaging with Multiple Synchrotron Beams
journal, March 2013

  • Stavitski, Eli; Smith, Randy J.; Bourassa, Megan W.
  • Analytical Chemistry, Vol. 85, Issue 7
  • DOI: 10.1021/ac3033849

Multi-wavelength mid-infrared plasmonic antennas with single nanoscale focal point
journal, January 2011

  • Blanchard, Romain; Boriskina, Svetlana V.; Genevet, Patrice
  • Optics Express, Vol. 19, Issue 22
  • DOI: 10.1364/OE.19.022113

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
journal, April 2010

  • Neumann, Beate; Walter, Thomas; Hériché, Jean-Karim
  • Nature, Vol. 464, Issue 7289
  • DOI: 10.1038/nature08869

Optimization of microfluidic systems for IRMS long term measurement of living cells
journal, October 2012


Fabrication of IR-transparent microfluidic devices by anisotropic etching of channels in CaF 2
journal, January 2015

  • Lehmkuhl, Brynson; Noblitt, Scott D.; Krummel, Amber T.
  • Lab on a Chip, Vol. 15, Issue 22
  • DOI: 10.1039/C5LC00759C

FTIR spectroscopy of single live cells in aqueous media by synchrotron IR microscopy using microfabricated sample holders
journal, May 2010


Water (H2O and D2O) Molar Absorptivity in the 1000–4000 cm−1Range and Quantitative Infrared Spectroscopy of Aqueous Solutions
journal, June 1997

  • Venyaminov, Sergei Yu.; Prendergast, Franklyn G.
  • Analytical Biochemistry, Vol. 248, Issue 2
  • DOI: 10.1006/abio.1997.2136

Three-dimensional sub–100 nm resolution fluorescence microscopy of thick samples
journal, May 2008

  • Juette, Manuel F.; Gould, Travis J.; Lessard, Mark D.
  • Nature Methods, Vol. 5, Issue 6
  • DOI: 10.1038/nmeth.1211

Subdiffraction Multicolor Imaging of the Nuclear Periphery with 3D Structured Illumination Microscopy
journal, June 2008


Determination of cell cycle phases in live B16 melanoma cells using IRMS
journal, January 2013

  • Bedolla, Diana E.; Kenig, Saša; Mitri, Elisa
  • The Analyst, Vol. 138, Issue 14
  • DOI: 10.1039/c3an00318c

Establishment and characterization of a human histiocytic lymphoma cell line (U-937)
journal, May 1976

  • Sundström, Christer; Nilsson, Kenneth
  • International Journal of Cancer, Vol. 17, Issue 5
  • DOI: 10.1002/ijc.2910170504

Fast Infrared Chemical Imaging with a Quantum Cascade Laser
journal, December 2014

  • Yeh, Kevin; Kenkel, Seth; Liu, Jui-Nung
  • Analytical Chemistry, Vol. 87, Issue 1
  • DOI: 10.1021/ac5027513

Synchrotron infrared spectromicroscopy as a novel bioanalytical microprobe for individual living cells: cytotoxicity considerations
journal, January 2002

  • Holman, Hoi-Ying N.; Bjornstad, Kathleen A.; McNamara, Morgan P.
  • Journal of Biomedical Optics, Vol. 7, Issue 3
  • DOI: 10.1117/1.1485299

Fabrication of a microfluidic platform for investigating dynamic biochemical processes in living samples by FTIR microspectroscopy
journal, May 2010

  • Birarda, Giovanni; Grenci, Gianluca; Businaro, Luca
  • Microelectronic Engineering, Vol. 87, Issue 5-8
  • DOI: 10.1016/j.mee.2009.11.081

Fourier Transform Spectroscopic Imaging Using an Infrared Focal-Plane Array Detector
journal, October 1995

  • Lewis, E. Neil.; Treado, Patrick J.; Reeder, Robert C.
  • Analytical Chemistry, Vol. 67, Issue 19
  • DOI: 10.1021/ac00115a003

Ultrahigh-resolution multicolor colocalization of single fluorescent probes
journal, August 2000

  • Lacoste, T. D.; Michalet, X.; Pinaud, F.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 17, p. 9461-9466
  • DOI: 10.1073/pnas.170286097

Demountable Liquid/Flow Cell for in vivo Infrared Microspectroscopy of Biological Specimens
journal, October 2009


Mapping of nutrient-induced biochemical changes in living algal cells using synchrotron infrared microspectroscopy
journal, August 2005


Apoptotic pathways of U937 leukemic monocytes investigated by infrared microspectroscopy and flow cytometry
journal, January 2014

  • Birarda, Giovanni; Bedolla, Diana E.; Mitri, Elisa
  • The Analyst, Vol. 139, Issue 12
  • DOI: 10.1039/C4AN00317A

Chemical imaging of biological tissue with synchrotron infrared light
journal, July 2006


Tomographic Phase Microscopy: Quantitative 3D-Mapping of Refractive Index in Live Cells
journal, March 2008


Works referencing / citing this record:

Interplay between materials and microfluidics
journal, April 2017

  • Hou, Xu; Zhang, Yu Shrike; Santiago, Grissel Trujillo-de
  • Nature Reviews Materials, Vol. 2, Issue 5
  • DOI: 10.1038/natrevmats.2017.16

Field-resolved infrared spectroscopy of biological systems
journal, January 2020


Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging
journal, January 2018

  • Azarfar, Ghazal; Aboualizadeh, Ebrahim; Walter, Nicholas M.
  • The Analyst, Vol. 143, Issue 19
  • DOI: 10.1039/c8an00093j

Synchrotron infrared nanospectroscopy on a graphene chip
journal, January 2019

  • Meireles, Leonel M.; Barcelos, Ingrid D.; Ferrari, Gustavo A.
  • Lab on a Chip, Vol. 19, Issue 21
  • DOI: 10.1039/c9lc00686a

Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples
journal, April 2017


Integrating Microfabrication into Biological Investigations: the Benefits of Interdisciplinarity
journal, April 2019

  • Grenci, Gianluca; Bertocchi, Cristina; Ravasio, Andrea
  • Micromachines, Vol. 10, Issue 4
  • DOI: 10.3390/mi10040252

Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging
text, January 2018


Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples
journal, April 2017


Integrating Microfabrication into Biological Investigations: the Benefits of Interdisciplinarity
journal, April 2019

  • Grenci, Gianluca; Bertocchi, Cristina; Ravasio, Andrea
  • Micromachines, Vol. 10, Issue 4
  • DOI: 10.3390/mi10040252