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Title: Integrated bioprocess for conversion of gaseous substrates to liquids

Abstract

In the quest for inexpensive feedstocks for the cost-effective production of liquid fuels, we have examined gaseous substrates that could be made available at low cost and sufficiently large scale for industrial fuel production. In this work, we introduce a new bioconversion scheme that effectively converts syngas, generated from gasification of coal, natural gas, or biomass, into lipids that can be used for biodiesel production. We present an integrated conversion method comprising a two-stage system. In the first stage, an anaerobic bioreactor converts mixtures of gases of CO2 and CO or H2 to acetic acid, using the anaerobic acetogen Moorella thermoacetica. The acetic acid product is fed as a substrate to a second bioreactor, where it is converted aerobically into lipids by an engineered oleaginous yeast, Yarrowia lipolytica. We first describe the process carried out in each reactor and then present an integrated system that produces microbial oil, using synthesis gas as input. The integrated continuous bench-scale reactor system produced 18 g/L of C16-C18 triacylglycerides directly from synthesis gas, with an overall productivity of 0.19 g•L-1•h-1 and a lipid content of 36%. Although suboptimal relative to the performance of the individual reactor components, the presented integrated system demonstrates the feasibilitymore » of substantial net fixation of carbon dioxide and conversion of gaseous feedstocks to lipids for biodiesel production. The system can be further optimized to approach the performance of its individual units so that it can be used for the economical conversion of waste gases from steel mills to valuable liquid fuels for transportation.« less

Authors:
 [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering; Tsinghua Univ., Beijing (China). Inst. of Nuclear and New Energy Technology
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division; USDOE Advanced Research Projects Agency - Energy (ARPA-E)
OSTI Identifier:
1469102
Grant/Contract Number:  
SC0008744; AR0000059; 692-6611
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 113; Journal Issue: 14; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; two-stage bioprocess; lipid production; microbial fermentation; gas-to-liquid fuel; CO2 fixation

Citation Formats

Hu, Peng, Chakraborty, Sagar, Kumar, Amit, Woolston, Benjamin, Liu, Hongjuan, Emerson, David, and Stephanopoulos, Gregory. Integrated bioprocess for conversion of gaseous substrates to liquids. United States: N. p., 2016. Web. doi:10.1073/pnas.1516867113.
Hu, Peng, Chakraborty, Sagar, Kumar, Amit, Woolston, Benjamin, Liu, Hongjuan, Emerson, David, & Stephanopoulos, Gregory. Integrated bioprocess for conversion of gaseous substrates to liquids. United States. doi:10.1073/pnas.1516867113.
Hu, Peng, Chakraborty, Sagar, Kumar, Amit, Woolston, Benjamin, Liu, Hongjuan, Emerson, David, and Stephanopoulos, Gregory. Mon . "Integrated bioprocess for conversion of gaseous substrates to liquids". United States. doi:10.1073/pnas.1516867113. https://www.osti.gov/servlets/purl/1469102.
@article{osti_1469102,
title = {Integrated bioprocess for conversion of gaseous substrates to liquids},
author = {Hu, Peng and Chakraborty, Sagar and Kumar, Amit and Woolston, Benjamin and Liu, Hongjuan and Emerson, David and Stephanopoulos, Gregory},
abstractNote = {In the quest for inexpensive feedstocks for the cost-effective production of liquid fuels, we have examined gaseous substrates that could be made available at low cost and sufficiently large scale for industrial fuel production. In this work, we introduce a new bioconversion scheme that effectively converts syngas, generated from gasification of coal, natural gas, or biomass, into lipids that can be used for biodiesel production. We present an integrated conversion method comprising a two-stage system. In the first stage, an anaerobic bioreactor converts mixtures of gases of CO2 and CO or H2 to acetic acid, using the anaerobic acetogen Moorella thermoacetica. The acetic acid product is fed as a substrate to a second bioreactor, where it is converted aerobically into lipids by an engineered oleaginous yeast, Yarrowia lipolytica. We first describe the process carried out in each reactor and then present an integrated system that produces microbial oil, using synthesis gas as input. The integrated continuous bench-scale reactor system produced 18 g/L of C16-C18 triacylglycerides directly from synthesis gas, with an overall productivity of 0.19 g•L-1•h-1 and a lipid content of 36%. Although suboptimal relative to the performance of the individual reactor components, the presented integrated system demonstrates the feasibility of substantial net fixation of carbon dioxide and conversion of gaseous feedstocks to lipids for biodiesel production. The system can be further optimized to approach the performance of its individual units so that it can be used for the economical conversion of waste gases from steel mills to valuable liquid fuels for transportation.},
doi = {10.1073/pnas.1516867113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 14,
volume = 113,
place = {United States},
year = {2016},
month = {3}
}

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