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Title: Combinations of Abiotic Factors Differentially Alter Production of Plant Secondary Metabolites in Five Woody Plant Species in the Boreal-Temperate Transition Zone

Abstract

Plant secondary metabolites (PSMs) are a key mechanism by which plants defend themselves against potential threats, and changes in the abiotic environment can alter the diversity and abundance of PSMs. While the number of studies investigating the effects of abiotic factors on PSM production is growing, we currently have a limited understanding of how combinations of factors may influence PSM production. The objective of this study was to determine how warming influences PSM production and how the addition of other factors may modulate this effect. We used untargeted metabolomics to evaluate how PSM production in five different woody plant species in northern Minnesota, United States are influenced by varying combinations of temperature, moisture, and light in both experimental and natural conditions. We also analyzed changes to the abundances of two compounds from two different species – two resin acids in Abies balsamea and catechin and a terpene acid in Betula papyrifera. We used permutational MANOVA to compare PSM profiles and phytochemical turnover across treatments and non-metric multidimensional scaling to visualize treatment-specific changes in PSM profiles. We used linear mixed-effects models to examine changes in phytochemical richness and changes in the abundances of our example compounds. Under closed-canopy, experimental warming ledmore » to distinct PSM profiles and induced phytochemical turnover in B. papyrifera. In open-canopy sites, warming had no influence on PSM production. In samples collected across northeastern Minnesota, regional temperature differences had no influence on PSM profiles or phytochemical richness but did induce phytochemical turnover in B. papyrifera and Populus tremuloides. However, warmer temperatures combined with open canopy resulted in distinct PSM profiles for all species and induced phytochemical turnover in all but Corylus cornuta. Although neither example compound in A . balsamea was influenced by any of the abiotic conditions, both compounds in B. papyrifera exhibited significant changes in response to warming and canopy. Our results demonstrate that the metabolic response of woody plants to combinations of abiotic factors cannot be extrapolated from that of a single factor and will differ by species. This heterogeneous phytochemical response directly affects interactions between plants and other organisms and may yield unexpected results as plant communities adapt to novel environmental conditions.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Minnesota, Minneapolis, MN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1469030
Alternate Identifier(s):
OSTI ID: 1510726
Grant/Contract Number:  
[FG02-07ER64456]
Resource Type:
Published Article
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
[Journal Name: Frontiers in Plant Science Journal Volume: 9]; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Media SA
Country of Publication:
Switzerland
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; phytochemical turnover; PSM diversity; untargeted metabolomics; balsam fir; beaked hazel; paper birch; red maple; trembling aspen

Citation Formats

Berini, John L., Brockman, Stephen A., Hegeman, Adrian D., Reich, Peter B., Muthukrishnan, Ranjan, Montgomery, Rebecca A., and Forester, James D. Combinations of Abiotic Factors Differentially Alter Production of Plant Secondary Metabolites in Five Woody Plant Species in the Boreal-Temperate Transition Zone. Switzerland: N. p., 2018. Web. doi:10.3389/fpls.2018.01257.
Berini, John L., Brockman, Stephen A., Hegeman, Adrian D., Reich, Peter B., Muthukrishnan, Ranjan, Montgomery, Rebecca A., & Forester, James D. Combinations of Abiotic Factors Differentially Alter Production of Plant Secondary Metabolites in Five Woody Plant Species in the Boreal-Temperate Transition Zone. Switzerland. doi:10.3389/fpls.2018.01257.
Berini, John L., Brockman, Stephen A., Hegeman, Adrian D., Reich, Peter B., Muthukrishnan, Ranjan, Montgomery, Rebecca A., and Forester, James D. Wed . "Combinations of Abiotic Factors Differentially Alter Production of Plant Secondary Metabolites in Five Woody Plant Species in the Boreal-Temperate Transition Zone". Switzerland. doi:10.3389/fpls.2018.01257.
@article{osti_1469030,
title = {Combinations of Abiotic Factors Differentially Alter Production of Plant Secondary Metabolites in Five Woody Plant Species in the Boreal-Temperate Transition Zone},
author = {Berini, John L. and Brockman, Stephen A. and Hegeman, Adrian D. and Reich, Peter B. and Muthukrishnan, Ranjan and Montgomery, Rebecca A. and Forester, James D.},
abstractNote = {Plant secondary metabolites (PSMs) are a key mechanism by which plants defend themselves against potential threats, and changes in the abiotic environment can alter the diversity and abundance of PSMs. While the number of studies investigating the effects of abiotic factors on PSM production is growing, we currently have a limited understanding of how combinations of factors may influence PSM production. The objective of this study was to determine how warming influences PSM production and how the addition of other factors may modulate this effect. We used untargeted metabolomics to evaluate how PSM production in five different woody plant species in northern Minnesota, United States are influenced by varying combinations of temperature, moisture, and light in both experimental and natural conditions. We also analyzed changes to the abundances of two compounds from two different species – two resin acids in Abies balsamea and catechin and a terpene acid in Betula papyrifera. We used permutational MANOVA to compare PSM profiles and phytochemical turnover across treatments and non-metric multidimensional scaling to visualize treatment-specific changes in PSM profiles. We used linear mixed-effects models to examine changes in phytochemical richness and changes in the abundances of our example compounds. Under closed-canopy, experimental warming led to distinct PSM profiles and induced phytochemical turnover in B. papyrifera. In open-canopy sites, warming had no influence on PSM production. In samples collected across northeastern Minnesota, regional temperature differences had no influence on PSM profiles or phytochemical richness but did induce phytochemical turnover in B. papyrifera and Populus tremuloides. However, warmer temperatures combined with open canopy resulted in distinct PSM profiles for all species and induced phytochemical turnover in all but Corylus cornuta. Although neither example compound in A. balsamea was influenced by any of the abiotic conditions, both compounds in B. papyrifera exhibited significant changes in response to warming and canopy. Our results demonstrate that the metabolic response of woody plants to combinations of abiotic factors cannot be extrapolated from that of a single factor and will differ by species. This heterogeneous phytochemical response directly affects interactions between plants and other organisms and may yield unexpected results as plant communities adapt to novel environmental conditions.},
doi = {10.3389/fpls.2018.01257},
journal = {Frontiers in Plant Science},
number = ,
volume = [9],
place = {Switzerland},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.3389/fpls.2018.01257

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Cited by: 3 works
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