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Title: X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam

Abstract

Here, X-ray Fluorescence (XRF) microscopy is a growing approach for imaging the trace element concentration, distribution, and speciation in biological cells at the nanoscale. Moreover, three-dimensional nanotomography provides the added advantage of imaging subcellular structure and chemical identity in three dimensions without the need for staining or sectioning of cells. To date, technical challenges in X-ray optics, sample preparation, and detection sensitivity have limited the use of XRF nanotomography in this area. Here, XRF nanotomography was used to image the elemental distribution in individual E. coli bacterial cells using a sub-15 nm beam at the Hard X-ray Nanoprobe beamline (HXN, 3-ID) at NSLS-II. These measurements were simultaneously combined with ptychography to image structural components of the cells. The cells were embedded in small (3–20 µm) sodium chloride crystals, which provided a non-aqueous matrix to retain the three-dimensional structure of the E. coli while collecting data at room temperature. Results showed a generally uniform distribution of calcium in the cells, but an inhomogeneous zinc distribution, most notably with concentrated regions of zinc at the polar ends of the cells. This work demonstrates that simultaneous two-dimensional ptychography and XRF nanotomography can be performed with a sub-15 nm beam size on unfrozen biologicalmore » cells to co-localize elemental distribution and nanostructure simultaneously.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1468367
Alternate Identifier(s):
OSTI ID: 1480977
Report Number(s):
BNL-209355-2018-JAAM
Journal ID: ISSN 2045-2322; 13415; PII: 31461
Grant/Contract Number:  
SC0012704
Resource Type:
Published Article
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Name: Scientific Reports Journal Volume: 8 Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; X-ray fluorescence; microscopy; nanotomography; ptychography; synchrotron; E. coli; cells; imaging

Citation Formats

Victor, Tiffany W., Easthon, Lindsey M., Ge, Mingyuan, O’Toole, Katherine H., Smith, Randy J., Huang, Xiaojing, Yan, Hanfei, Allen, Karen N., Chu, Yong S., and Miller, Lisa M. X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam. United Kingdom: N. p., 2018. Web. doi:10.1038/s41598-018-31461-y.
Victor, Tiffany W., Easthon, Lindsey M., Ge, Mingyuan, O’Toole, Katherine H., Smith, Randy J., Huang, Xiaojing, Yan, Hanfei, Allen, Karen N., Chu, Yong S., & Miller, Lisa M. X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam. United Kingdom. doi:10.1038/s41598-018-31461-y.
Victor, Tiffany W., Easthon, Lindsey M., Ge, Mingyuan, O’Toole, Katherine H., Smith, Randy J., Huang, Xiaojing, Yan, Hanfei, Allen, Karen N., Chu, Yong S., and Miller, Lisa M. Fri . "X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam". United Kingdom. doi:10.1038/s41598-018-31461-y.
@article{osti_1468367,
title = {X-ray Fluorescence Nanotomography of Single Bacteria with a Sub-15 nm Beam},
author = {Victor, Tiffany W. and Easthon, Lindsey M. and Ge, Mingyuan and O’Toole, Katherine H. and Smith, Randy J. and Huang, Xiaojing and Yan, Hanfei and Allen, Karen N. and Chu, Yong S. and Miller, Lisa M.},
abstractNote = {Here, X-ray Fluorescence (XRF) microscopy is a growing approach for imaging the trace element concentration, distribution, and speciation in biological cells at the nanoscale. Moreover, three-dimensional nanotomography provides the added advantage of imaging subcellular structure and chemical identity in three dimensions without the need for staining or sectioning of cells. To date, technical challenges in X-ray optics, sample preparation, and detection sensitivity have limited the use of XRF nanotomography in this area. Here, XRF nanotomography was used to image the elemental distribution in individual E. coli bacterial cells using a sub-15 nm beam at the Hard X-ray Nanoprobe beamline (HXN, 3-ID) at NSLS-II. These measurements were simultaneously combined with ptychography to image structural components of the cells. The cells were embedded in small (3–20 µm) sodium chloride crystals, which provided a non-aqueous matrix to retain the three-dimensional structure of the E. coli while collecting data at room temperature. Results showed a generally uniform distribution of calcium in the cells, but an inhomogeneous zinc distribution, most notably with concentrated regions of zinc at the polar ends of the cells. This work demonstrates that simultaneous two-dimensional ptychography and XRF nanotomography can be performed with a sub-15 nm beam size on unfrozen biological cells to co-localize elemental distribution and nanostructure simultaneously.},
doi = {10.1038/s41598-018-31461-y},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United Kingdom},
year = {2018},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1038/s41598-018-31461-y

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Cited by: 3 works
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