Complete genome sequence of Pseudomonas stutzeri strain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site
Abstract
Hexavalent Chromium [Cr(VI)] is a widespread contaminant found in soil, sediment, and ground water in several DOE sites, including Hanford 100 H area. In order to stimulate microbially mediated reduction of Cr(VI) at this site, a poly-lactate hydrogen release compound was injected into the chromium contaminated aquifer. Targeted enrichment of dominant nitrate-reducing bacteria post injection resulted in the isolation of Pseudomonas stutzeri strain RCH2. P. stutzeri strain RCH2 was isolated using acetate as the electron donor and is a complete denitrifier. Experiments with anaerobic washed cell suspension of strain RCH2 revealed it could reduce Cr(VI) and Fe(III). The genome of strain RCH2 was sequenced using a combination of Illumina and 454 sequencing technologies and contained a circular chromosome of 4.6 Mb and three plasmids. Global genome comparisons of strain RCH2 with six other fully sequenced P. stutzeri strains revealed most genomic regions are conserved, however strain RCH2 has an additional 244 genes, some of which are involved in chemotaxis, Flp pilus biogenesis and pyruvate/2-oxogluturate complex formation.
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1618962
- Alternate Identifier(s):
- OSTI ID: 1354676; OSTI ID: 1379726; OSTI ID: 1468125
- Grant/Contract Number:
- AC02-05CH1123; AC05-00OR22725; AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Standards in Genomic Sciences
- Additional Journal Information:
- Journal Name: Standards in Genomic Sciences Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1944-3277
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; pseudomonas; nitrate reduction; chromium; Hanford 100H
Citation Formats
Chakraborty, Romy, Woo, Hannah, Dehal, Paramvir, Walker, Robert, Zemla, Marcin, Auer, Manfred, Goodwin, Lynne A., Kazakov, Alexey, Novichkov, Pavel, Arkin, Adam P., and Hazen, Terry C. Complete genome sequence of Pseudomonas stutzeri strain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site. United Kingdom: N. p., 2017.
Web. doi:10.1186/s40793-017-0233-7.
Chakraborty, Romy, Woo, Hannah, Dehal, Paramvir, Walker, Robert, Zemla, Marcin, Auer, Manfred, Goodwin, Lynne A., Kazakov, Alexey, Novichkov, Pavel, Arkin, Adam P., & Hazen, Terry C. Complete genome sequence of Pseudomonas stutzeri strain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site. United Kingdom. https://doi.org/10.1186/s40793-017-0233-7
Chakraborty, Romy, Woo, Hannah, Dehal, Paramvir, Walker, Robert, Zemla, Marcin, Auer, Manfred, Goodwin, Lynne A., Kazakov, Alexey, Novichkov, Pavel, Arkin, Adam P., and Hazen, Terry C. Wed .
"Complete genome sequence of Pseudomonas stutzeri strain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site". United Kingdom. https://doi.org/10.1186/s40793-017-0233-7.
@article{osti_1618962,
title = {Complete genome sequence of Pseudomonas stutzeri strain RCH2 isolated from a Hexavalent Chromium [Cr(VI)] contaminated site},
author = {Chakraborty, Romy and Woo, Hannah and Dehal, Paramvir and Walker, Robert and Zemla, Marcin and Auer, Manfred and Goodwin, Lynne A. and Kazakov, Alexey and Novichkov, Pavel and Arkin, Adam P. and Hazen, Terry C.},
abstractNote = {Hexavalent Chromium [Cr(VI)] is a widespread contaminant found in soil, sediment, and ground water in several DOE sites, including Hanford 100 H area. In order to stimulate microbially mediated reduction of Cr(VI) at this site, a poly-lactate hydrogen release compound was injected into the chromium contaminated aquifer. Targeted enrichment of dominant nitrate-reducing bacteria post injection resulted in the isolation of Pseudomonas stutzeri strain RCH2. P. stutzeri strain RCH2 was isolated using acetate as the electron donor and is a complete denitrifier. Experiments with anaerobic washed cell suspension of strain RCH2 revealed it could reduce Cr(VI) and Fe(III). The genome of strain RCH2 was sequenced using a combination of Illumina and 454 sequencing technologies and contained a circular chromosome of 4.6 Mb and three plasmids. Global genome comparisons of strain RCH2 with six other fully sequenced P. stutzeri strains revealed most genomic regions are conserved, however strain RCH2 has an additional 244 genes, some of which are involved in chemotaxis, Flp pilus biogenesis and pyruvate/2-oxogluturate complex formation.},
doi = {10.1186/s40793-017-0233-7},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 12,
place = {United Kingdom},
year = {Wed Feb 08 00:00:00 EST 2017},
month = {Wed Feb 08 00:00:00 EST 2017}
}
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https://doi.org/10.1186/s40793-017-0233-7
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