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Title: Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis

Abstract

Local interactions between individual plant organs and diverse microorganisms can lead to whole plant immunity via the mobilization of defense signals. One such signal is the plastid lipid-derived oxylipin azelaic acid (AZA). Arabidopsis lacking AZI1 or EARLI1, related lipid transfer family proteins, exhibit reduced AZA transport among leaves and cannot mount systemic immunity. AZA has been detected in roots as well as leaves. Thus, the present study addresses the effects on plants of AZA application to roots. AZA, but not the structurally related suberic acid, inhibits root growth when directly in contact with roots. Treatment of roots with AZA also induces resistance to Pseudomonas syringae in aerial tissues. Additionally, these effects of AZA on root growth and disease resistance depend at least partially on AZI1 and EARLI1. AZI1 in roots localizes to plastids, similar to its known location in leaves. Interestingly, kinases previously shown to modify AZI1 in vitro, MPK3/6, are also needed for AZA-induced root growth inhibition and above ground immunity. Finally, [2H]-AZA applied to the roots does not move to aerial tissues. Therefore, AZA application to roots triggers systemic immunity through an AZI1/EARLI1/MPK3/MPK6-dependent pathway and AZA’s effects may involve an additional mobile signal(s).

Authors:
 [1];  [2];  [3];  [2];  [2];  [2];  [2];  [4];  [5]; ORCiD logo [5];  [2]
  1. Ciudad Universitaria Universidad Nacional de Córdoba (Argentina). Centro de Investigaciones en Quimica Biologica de Cordoba
  2. Univ. of Chicago, IL (United States). Division of Biological Sciences
  3. Univ. of Chicago, IL (United States). Molecular Genetics and Cell Biology
  4. Dong-A University (Korea). Department of Genetic Engineering
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1468048
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Molecular Plant-Microbe Interactions
Additional Journal Information:
Journal Volume: 32; Journal Issue: 1; Journal ID: ISSN 0894-0282
Publisher:
APS Press - International Society for Molecular Plant-Microbe Interactions
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Cecchini, Nicolas M., Roychoudhry, Suruchi, Speed, Dequantarius J., Steffes, Kevin, Tambe, Arjun, Zodrow, Kristin, Konstantinoff, Katerina, JUNG, HO WON, Engle, Nancy, Tschaplinski, Timothy J., and Greenberg, Jean. Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. United States: N. p., 2018. Web. doi:10.1094/MPMI-07-18-0185-R.
Cecchini, Nicolas M., Roychoudhry, Suruchi, Speed, Dequantarius J., Steffes, Kevin, Tambe, Arjun, Zodrow, Kristin, Konstantinoff, Katerina, JUNG, HO WON, Engle, Nancy, Tschaplinski, Timothy J., & Greenberg, Jean. Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. United States. doi:10.1094/MPMI-07-18-0185-R.
Cecchini, Nicolas M., Roychoudhry, Suruchi, Speed, Dequantarius J., Steffes, Kevin, Tambe, Arjun, Zodrow, Kristin, Konstantinoff, Katerina, JUNG, HO WON, Engle, Nancy, Tschaplinski, Timothy J., and Greenberg, Jean. Wed . "Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis". United States. doi:10.1094/MPMI-07-18-0185-R. https://www.osti.gov/servlets/purl/1468048.
@article{osti_1468048,
title = {Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis},
author = {Cecchini, Nicolas M. and Roychoudhry, Suruchi and Speed, Dequantarius J. and Steffes, Kevin and Tambe, Arjun and Zodrow, Kristin and Konstantinoff, Katerina and JUNG, HO WON and Engle, Nancy and Tschaplinski, Timothy J. and Greenberg, Jean},
abstractNote = {Local interactions between individual plant organs and diverse microorganisms can lead to whole plant immunity via the mobilization of defense signals. One such signal is the plastid lipid-derived oxylipin azelaic acid (AZA). Arabidopsis lacking AZI1 or EARLI1, related lipid transfer family proteins, exhibit reduced AZA transport among leaves and cannot mount systemic immunity. AZA has been detected in roots as well as leaves. Thus, the present study addresses the effects on plants of AZA application to roots. AZA, but not the structurally related suberic acid, inhibits root growth when directly in contact with roots. Treatment of roots with AZA also induces resistance to Pseudomonas syringae in aerial tissues. Additionally, these effects of AZA on root growth and disease resistance depend at least partially on AZI1 and EARLI1. AZI1 in roots localizes to plastids, similar to its known location in leaves. Interestingly, kinases previously shown to modify AZI1 in vitro, MPK3/6, are also needed for AZA-induced root growth inhibition and above ground immunity. Finally, [2H]-AZA applied to the roots does not move to aerial tissues. Therefore, AZA application to roots triggers systemic immunity through an AZI1/EARLI1/MPK3/MPK6-dependent pathway and AZA’s effects may involve an additional mobile signal(s).},
doi = {10.1094/MPMI-07-18-0185-R},
journal = {Molecular Plant-Microbe Interactions},
number = 1,
volume = 32,
place = {United States},
year = {2018},
month = {8}
}

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Figures / Tables:

Table 1 Table 1: Root uptake and transport of [2H]azelaic acid in WT (Col-0), azi1-1 and earli1- 1 plants.

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.