Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1
Abstract
WRINKLED1 (WRI1), the transcriptional activator of fatty acid synthesis, was recently identified as a target of KIN10, a catalytic α-subunit of the SUCROSE-NON-FERMENTING-1-RELATED PROTEIN KINASE-1 (SnRK1). For this work, we tested the hypothesis that trehalose 6-phosphate (T6P), a signal of cellular sucrose status, can regulate FA synthesis by inhibiting SnRK1. Incubation of Brassica napus suspension cells in medium containing T6P, or overexpression of the Escherichia coli T6P synthase, OtsA, in tobacco, significantly increased T6P levels, WRI1 levels and FA synthesis rates. T6P directly bound to purified recombinant KIN10 with an equilibrium dissociation constant (Kd) of 32 ± 6 μM using microscale thermophoresis. GEMINIVIRUS REP-INTERACTING KINASE1 (GRIK1) bound to KIN10 (Kd 19 ± 3 μM) and activated it by phosphorylation. In the presence of T6P the GRIK1-KIN10 association was weakened by more than threefold (Kd 67.7 ± 9.8 μM) which reduced both the phosphorylation of KIN10 and its activity. T6P-dependent inhibition of SnRK1 activity is reduced in extracts of individual Arabidopsis grik1 and grik2 mutants relative to wild type, while SnRK1 activity in grik1grik2 extracts is enhanced by T6P. These results indicate the T6P sensitivity of SnRK1 in vivo is GRIK1/GRIK2-dependent. A mechanistic model is proposed that links sugar signaling andmore »
- Authors:
-
- Department of Biology, Brookhaven National Laboratory, Upton, New York 11973
- Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
- OSTI Identifier:
- 1472222
- Alternate Identifier(s):
- OSTI ID: 1467850
- Report Number(s):
- BNL-209015-2018-JAAM
Journal ID: ISSN 1040-4651; /plantcell/30/10/2616.atom
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Published Article
- Journal Name:
- Plant Cell
- Additional Journal Information:
- Journal Name: Plant Cell Journal Volume: 30 Journal Issue: 10; Journal ID: ISSN 1040-4651
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Zhai, Zhiyang, Keereetaweep, Jantana, Liu, Hui, Feil, Regina, Lunn, John E., and Shanklin, John. Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1. United States: N. p., 2018.
Web. doi:10.1105/tpc.18.00521.
Zhai, Zhiyang, Keereetaweep, Jantana, Liu, Hui, Feil, Regina, Lunn, John E., & Shanklin, John. Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1. United States. https://doi.org/10.1105/tpc.18.00521
Zhai, Zhiyang, Keereetaweep, Jantana, Liu, Hui, Feil, Regina, Lunn, John E., and Shanklin, John. Mon .
"Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1". United States. https://doi.org/10.1105/tpc.18.00521.
@article{osti_1472222,
title = {Trehalose 6-Phosphate Positively Regulates Fatty Acid Synthesis by Stabilizing WRINKLED1},
author = {Zhai, Zhiyang and Keereetaweep, Jantana and Liu, Hui and Feil, Regina and Lunn, John E. and Shanklin, John},
abstractNote = {WRINKLED1 (WRI1), the transcriptional activator of fatty acid synthesis, was recently identified as a target of KIN10, a catalytic α-subunit of the SUCROSE-NON-FERMENTING-1-RELATED PROTEIN KINASE-1 (SnRK1). For this work, we tested the hypothesis that trehalose 6-phosphate (T6P), a signal of cellular sucrose status, can regulate FA synthesis by inhibiting SnRK1. Incubation of Brassica napus suspension cells in medium containing T6P, or overexpression of the Escherichia coli T6P synthase, OtsA, in tobacco, significantly increased T6P levels, WRI1 levels and FA synthesis rates. T6P directly bound to purified recombinant KIN10 with an equilibrium dissociation constant (Kd) of 32 ± 6 μM using microscale thermophoresis. GEMINIVIRUS REP-INTERACTING KINASE1 (GRIK1) bound to KIN10 (Kd 19 ± 3 μM) and activated it by phosphorylation. In the presence of T6P the GRIK1-KIN10 association was weakened by more than threefold (Kd 67.7 ± 9.8 μM) which reduced both the phosphorylation of KIN10 and its activity. T6P-dependent inhibition of SnRK1 activity is reduced in extracts of individual Arabidopsis grik1 and grik2 mutants relative to wild type, while SnRK1 activity in grik1grik2 extracts is enhanced by T6P. These results indicate the T6P sensitivity of SnRK1 in vivo is GRIK1/GRIK2-dependent. A mechanistic model is proposed that links sugar signaling and FA homeostasis.},
doi = {10.1105/tpc.18.00521},
journal = {Plant Cell},
number = 10,
volume = 30,
place = {United States},
year = {Mon Sep 24 00:00:00 EDT 2018},
month = {Mon Sep 24 00:00:00 EDT 2018}
}
https://doi.org/10.1105/tpc.18.00521
Web of Science
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