Synchrotron-based X-ray Fluorescence Microscopy as a Technique for Imaging of Elements in Plants
Abstract
Understanding the distribution of elements within plant tissues is important across a range of fields in plant science. In this review, we examine synchrotron-based X-ray fluorescence microscopy (XFM) as an elemental imaging technique in plant sciences, considering both its historical and current uses as well as discussing emerging approaches. XFM offers several unique capabilities of interest to plant scientists, including in vivo analyses at room temperature and pressure, good detection limits (ca. 1-100 mg/kg), and excellent resolution (down to 50 nm). This has permitted its use in a range of studies, including for functional characterization in molecular biology, examining the distribution of nutrients in food products, understanding movement of foliar fertilizers, investigating the behaviour of engineered nanoparticles, elucidating the toxic effects of metal(loid)s in agronomic plant species, and studying the unique properties of hyperaccumulating plants. We anticipate that continuing technological advances at XFM beamlines will also provide new opportunities moving into the future, such as for high-throughput screening in molecular biology, use of exotic metal tags for protein localization, and for enabling time-resolved, in vivo analyses of living plants. By examining current and potential future applications, we hope to encourage further XFM studies in plant sciences by highlighting the versatilitymore »
- Authors:
-
- Univ. of Queensland, St. Lucia, QLD (Australia). School of Agriculture and Food Sciences
- Dartmouth College, Hanover, NH (United States). Dept. of Biological Sciences and Life Science Center
- Australian Nuclear Science and Technology Organisation (ANSTO), Melbourne, VIC (Australia). Australian Synchrotron
- Brookhaven National Lab. (BNL), Upton, NY (United States). Photon Sciences Division
- Nanjing Agricultural Univ. (China). College of Resources and Environmental Sciences
- Univ. of Queensland, St. Lucia, QLD (Australia). School of Agriculture and Food Sciences and Centre for Soil and Environmental Research
- Univ. of Queensland, St. Lucia, QLD (Australia). Centre for Mined Land Rehabilitation and Sustainable Minerals Inst.
- Univ. of South Australia, Mawson Lakes, SA (Australia). Future Industries Inst.
- Publication Date:
- Research Org.:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); Australian Research Council (ARC)
- Contributing Org.:
- Commonwealth Scientific and Industrial Research Organisation (CSIRO), Canberra (Australia)
- OSTI Identifier:
- 1467848
- Report Number(s):
- BNL-208017-2018-JAAM
- Grant/Contract Number:
- SC0012704; 160100429
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Physiology
- Additional Journal Information:
- Journal Volume: 177; Journal Issue: 4
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 47 OTHER INSTRUMENTATION; elemental imaging; in vivo and in situ analyses; mapping; x-ray fluorescence microscopy
Citation Formats
Kopittke, Peter M., Punshon, Tracy, Paterson, David J., Tappero, Ryan V., Wang, Peng, Blamey, Pax, van der Ent, Antony, and Lombi, Enzo. Synchrotron-based X-ray Fluorescence Microscopy as a Technique for Imaging of Elements in Plants. United States: N. p., 2018.
Web. doi:10.1104/pp.18.00759.
Kopittke, Peter M., Punshon, Tracy, Paterson, David J., Tappero, Ryan V., Wang, Peng, Blamey, Pax, van der Ent, Antony, & Lombi, Enzo. Synchrotron-based X-ray Fluorescence Microscopy as a Technique for Imaging of Elements in Plants. United States. https://doi.org/10.1104/pp.18.00759
Kopittke, Peter M., Punshon, Tracy, Paterson, David J., Tappero, Ryan V., Wang, Peng, Blamey, Pax, van der Ent, Antony, and Lombi, Enzo. Tue .
"Synchrotron-based X-ray Fluorescence Microscopy as a Technique for Imaging of Elements in Plants". United States. https://doi.org/10.1104/pp.18.00759. https://www.osti.gov/servlets/purl/1467848.
@article{osti_1467848,
title = {Synchrotron-based X-ray Fluorescence Microscopy as a Technique for Imaging of Elements in Plants},
author = {Kopittke, Peter M. and Punshon, Tracy and Paterson, David J. and Tappero, Ryan V. and Wang, Peng and Blamey, Pax and van der Ent, Antony and Lombi, Enzo},
abstractNote = {Understanding the distribution of elements within plant tissues is important across a range of fields in plant science. In this review, we examine synchrotron-based X-ray fluorescence microscopy (XFM) as an elemental imaging technique in plant sciences, considering both its historical and current uses as well as discussing emerging approaches. XFM offers several unique capabilities of interest to plant scientists, including in vivo analyses at room temperature and pressure, good detection limits (ca. 1-100 mg/kg), and excellent resolution (down to 50 nm). This has permitted its use in a range of studies, including for functional characterization in molecular biology, examining the distribution of nutrients in food products, understanding movement of foliar fertilizers, investigating the behaviour of engineered nanoparticles, elucidating the toxic effects of metal(loid)s in agronomic plant species, and studying the unique properties of hyperaccumulating plants. We anticipate that continuing technological advances at XFM beamlines will also provide new opportunities moving into the future, such as for high-throughput screening in molecular biology, use of exotic metal tags for protein localization, and for enabling time-resolved, in vivo analyses of living plants. By examining current and potential future applications, we hope to encourage further XFM studies in plant sciences by highlighting the versatility of this approach.},
doi = {10.1104/pp.18.00759},
journal = {Plant Physiology},
number = 4,
volume = 177,
place = {United States},
year = {Tue Aug 14 00:00:00 EDT 2018},
month = {Tue Aug 14 00:00:00 EDT 2018}
}
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Works referencing / citing this record:
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