Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women
Abstract
Urine and serum biomonitoring was used to measure internal exposure to selected dietary estrogens in a cohort of 30 pregnant women. Herein exposure was measured over a period comprising one-half day in the field (6 h) and one day in a clinic (24 h). Biomonitoring of the dietary phytoestrogens genistein (GEN), daidzein (DDZ) and equol (EQ), as well as the mycoestrogen, zearalenone (ZEN) and its congeners, was conducted using UPLC-MS/MS. Biomonitoring revealed evidence of internal exposure to naturally occurring dietary estrogens during pregnancy. Urinary concentrations of total GEN, DDZ and EQ were similar to levels reported for general adult U.S. population. Measurable concentrations of total (parent and metabolites) GEN, DDZ and EQ were present in 240, 207 and 2 of 270 serum samples, respectively. Six out of 30 subjects had measurable concentrations of unconjugated GEN and/or DDZ in serum between 0.6 and 7.1 nM. Urine to serum total isoflavone ratios for GEN, DDZ and EQ were 13, 47, and 180, respectively. ZEN and its reductive metabolite, a-zearalenol (a-ZEL), were present in pregnant women (11 out of 30 subjects) as conjugates at levels near the limit of quantification. The average total urinary concentration was 0.10 mg/L for ZEN and 0.11 mg/Lmore »
- Authors:
-
- U.S. Food and Drug Administration (FDA), Jefferson, AR (United States)
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Oregon State Univ., Corvallis, OR (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE; USDA; American Chemistry Council
- OSTI Identifier:
- 1988267
- Alternate Identifier(s):
- OSTI ID: 1467737
- Report Number(s):
- PNNL-ACT-SA-10583
Journal ID: ISSN 0278-6915
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Food and Chemical Toxicology
- Additional Journal Information:
- Journal Volume: 95; Journal ID: ISSN 0278-6915
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; soy isoflavones; zearalenone; exposure; pregnancy; biomonitoring; endocrine disruptors
Citation Formats
Fleck, Stefanie C., Churchwell, Mona I., Doerge, Daniel R., and Teeguarden, Justin G. Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women. United States: N. p., 2016.
Web. doi:10.1016/j.fct.2016.05.021.
Fleck, Stefanie C., Churchwell, Mona I., Doerge, Daniel R., & Teeguarden, Justin G. Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women. United States. https://doi.org/10.1016/j.fct.2016.05.021
Fleck, Stefanie C., Churchwell, Mona I., Doerge, Daniel R., and Teeguarden, Justin G. Mon .
"Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women". United States. https://doi.org/10.1016/j.fct.2016.05.021. https://www.osti.gov/servlets/purl/1988267.
@article{osti_1988267,
title = {Urine and serum biomonitoring of exposure to environmental estrogens II: Soy isoflavones and zearalenone in pregnant women},
author = {Fleck, Stefanie C. and Churchwell, Mona I. and Doerge, Daniel R. and Teeguarden, Justin G.},
abstractNote = {Urine and serum biomonitoring was used to measure internal exposure to selected dietary estrogens in a cohort of 30 pregnant women. Herein exposure was measured over a period comprising one-half day in the field (6 h) and one day in a clinic (24 h). Biomonitoring of the dietary phytoestrogens genistein (GEN), daidzein (DDZ) and equol (EQ), as well as the mycoestrogen, zearalenone (ZEN) and its congeners, was conducted using UPLC-MS/MS. Biomonitoring revealed evidence of internal exposure to naturally occurring dietary estrogens during pregnancy. Urinary concentrations of total GEN, DDZ and EQ were similar to levels reported for general adult U.S. population. Measurable concentrations of total (parent and metabolites) GEN, DDZ and EQ were present in 240, 207 and 2 of 270 serum samples, respectively. Six out of 30 subjects had measurable concentrations of unconjugated GEN and/or DDZ in serum between 0.6 and 7.1 nM. Urine to serum total isoflavone ratios for GEN, DDZ and EQ were 13, 47, and 180, respectively. ZEN and its reductive metabolite, a-zearalenol (a-ZEL), were present in pregnant women (11 out of 30 subjects) as conjugates at levels near the limit of quantification. The average total urinary concentration was 0.10 mg/L for ZEN and 0.11 mg/L for a-ZEL.},
doi = {10.1016/j.fct.2016.05.021},
journal = {Food and Chemical Toxicology},
number = ,
volume = 95,
place = {United States},
year = {Mon May 30 00:00:00 EDT 2016},
month = {Mon May 30 00:00:00 EDT 2016}
}
Web of Science
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