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Title: Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi

Abstract

High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.

Authors:
 [1]; ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1];  [3];  [1];  [1];  [4];  [1];  [1]; ORCiD logo [5]; ORCiD logo [6]; ORCiD logo [7]; ORCiD logo [8]; ORCiD logo [1]
  1. Max Planck Inst. for Chemical Ecology, Jena (Germany). Dept. of Molecular Ecology
  2. Max Planck Inst. for Chemical Ecology, Jena (Germany). Dept. of Bioorganic Chemistry
  3. Max Planck Inst. for Chemical Ecology, Jena (Germany). Research Group Biosynthesis / NMR
  4. Max Planck Inst. for Chemical Ecology, Jena (Germany). Dept. of Molecular Ecology; Univ. of Chinese Academy of Sciences, Beijing (China). College of Life Sciences
  5. Leibniz-Inst.of Vegetable and Ornamental Crops, Grossbeeren (Germany); Humboldt Univ. zu Berlin, Berlin (Germany). Inst. of Biology
  6. Leibniz-Inst.of Vegetable and Ornamental Crops, Grossbeeren (Germany)
  7. Boyce Thompson Inst. for Plant Research, Ithaca (United States)
  8. Univ. of Cambridge, Cambridge (United Kingdom). Dept. of Plant Sciences
Publication Date:
Research Org.:
Boyce Thompson Inst., Ithaca, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1467130
Alternate Identifier(s):
OSTI ID: 1467131; OSTI ID: 1509808
Grant/Contract Number:  
SC0012460; 293926
Resource Type:
Published Article
Journal Name:
eLife
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2050-084X
Publisher:
eLife Sciences Publications, Ltd.
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wang, Ming, Schäfer, Martin, Li, Dapeng, Halitschke, Rayko, Dong, Chuanfu, McGale, Erica, Paetz, Christian, Song, Yuanyuan, Li, Suhua, Dong, Junfu, Heiling, Sven, Groten, Karin, Franken, Philipp, Bitterlich, Michael, Harrison, Maria J., Paszkowski, Uta, and Baldwin, Ian T. Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi. United States: N. p., 2018. Web. doi:10.7554/elife.37093.
Wang, Ming, Schäfer, Martin, Li, Dapeng, Halitschke, Rayko, Dong, Chuanfu, McGale, Erica, Paetz, Christian, Song, Yuanyuan, Li, Suhua, Dong, Junfu, Heiling, Sven, Groten, Karin, Franken, Philipp, Bitterlich, Michael, Harrison, Maria J., Paszkowski, Uta, & Baldwin, Ian T. Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi. United States. doi:10.7554/elife.37093.
Wang, Ming, Schäfer, Martin, Li, Dapeng, Halitschke, Rayko, Dong, Chuanfu, McGale, Erica, Paetz, Christian, Song, Yuanyuan, Li, Suhua, Dong, Junfu, Heiling, Sven, Groten, Karin, Franken, Philipp, Bitterlich, Michael, Harrison, Maria J., Paszkowski, Uta, and Baldwin, Ian T. Tue . "Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi". United States. doi:10.7554/elife.37093.
@article{osti_1467130,
title = {Blumenols as shoot markers of root symbiosis with arbuscular mycorrhizal fungi},
author = {Wang, Ming and Schäfer, Martin and Li, Dapeng and Halitschke, Rayko and Dong, Chuanfu and McGale, Erica and Paetz, Christian and Song, Yuanyuan and Li, Suhua and Dong, Junfu and Heiling, Sven and Groten, Karin and Franken, Philipp and Bitterlich, Michael and Harrison, Maria J. and Paszkowski, Uta and Baldwin, Ian T.},
abstractNote = {High-through-put (HTP) screening for functional arbuscular mycorrhizal fungi (AMF)-associations is challenging because roots must be excavated and colonization evaluated by transcript analysis or microscopy. Here we show that specific leaf-metabolites provide broadly applicable accurate proxies of these associations, suitable for HTP-screens. With a combination of untargeted and targeted metabolomics, we show that shoot accumulations of hydroxy- and carboxyblumenol C-glucosides mirror root AMF-colonization in Nicotiana attenuata plants. Genetic/pharmacologic manipulations indicate that these AMF-indicative foliar blumenols are synthesized and transported from roots to shoots. These blumenol-derived foliar markers, found in many di- and monocotyledonous crop and model plants (Solanum lycopersicum, Solanum tuberosum, Hordeum vulgare, Triticum aestivum, Medicago truncatula and Brachypodium distachyon), are not restricted to particular plant-AMF interactions, and are shown to be applicable for field-based QTL mapping of AMF-related genes.},
doi = {10.7554/elife.37093},
journal = {eLife},
number = ,
volume = 7,
place = {United States},
year = {2018},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.7554/elife.37093

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