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Title: Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors

Abstract

The tools for optically imaging cellular potassium concentrations in real-time are currently limited to a small set of molecular indicator dyes. Quantum dot-based nanosensors are more photostable and tunable than organic indicators, but previous designs have fallen short in size, sensitivity, and selectivity. In this paper, we introduce a small, sensitive, and selective nanosensor for potassium measurements. A dynamic quencher modulates the fluorescence emitted by two different quantum dot species to produce a ratiometric signal. We characterized the potassium-modulated sensor properties and investigated the photonic interactions within the sensors. The quencher’s protonation changes in response to potassium, which modulates its Förster radiative energy transfer rate and the corresponding interaction radii with each quantum dot species. Finally, the nanosensors respond to changes in potassium concentrations typical of the cellular environment and thus provide a promising tool for imaging potassium fluxes during biological events.

Authors:
 [1];  [1];  [2];  [3];  [2];  [3];  [1]
  1. Northeastern Univ., Boston, MA (United States). Dept. of Pharmaceutical Sciences
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Electrical Engineering
  3. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Materials Science and Engineering
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Northeastern Univ., Boston, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH) (United States); National Academy of Sciences Ford Foundation (United States)
OSTI Identifier:
1467074
Grant/Contract Number:  
FG02-07ER46474; R01NS081641; F32EB015847; 2014-PGA084488
Resource Type:
Accepted Manuscript
Journal Name:
ACS Nano
Additional Journal Information:
Journal Volume: 10; Journal Issue: 4; Journal ID: ISSN 1936-0851
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; FRET; ion sensing; nanosensor; optode; quantum dot

Citation Formats

Ruckh, Timothy T., Skipwith, Christopher G., Chang, Wendi, Senko, Alexander W., Bulovic, Vladimir, Anikeeva, Polina O., and Clark, Heather A. Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors. United States: N. p., 2016. Web. doi:10.1021/acsnano.5b05396.
Ruckh, Timothy T., Skipwith, Christopher G., Chang, Wendi, Senko, Alexander W., Bulovic, Vladimir, Anikeeva, Polina O., & Clark, Heather A. Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors. United States. doi:10.1021/acsnano.5b05396.
Ruckh, Timothy T., Skipwith, Christopher G., Chang, Wendi, Senko, Alexander W., Bulovic, Vladimir, Anikeeva, Polina O., and Clark, Heather A. Mon . "Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors". United States. doi:10.1021/acsnano.5b05396. https://www.osti.gov/servlets/purl/1467074.
@article{osti_1467074,
title = {Ion-Switchable Quantum Dot Förster Resonance Energy Transfer Rates in Ratiometric Potassium Sensors},
author = {Ruckh, Timothy T. and Skipwith, Christopher G. and Chang, Wendi and Senko, Alexander W. and Bulovic, Vladimir and Anikeeva, Polina O. and Clark, Heather A.},
abstractNote = {The tools for optically imaging cellular potassium concentrations in real-time are currently limited to a small set of molecular indicator dyes. Quantum dot-based nanosensors are more photostable and tunable than organic indicators, but previous designs have fallen short in size, sensitivity, and selectivity. In this paper, we introduce a small, sensitive, and selective nanosensor for potassium measurements. A dynamic quencher modulates the fluorescence emitted by two different quantum dot species to produce a ratiometric signal. We characterized the potassium-modulated sensor properties and investigated the photonic interactions within the sensors. The quencher’s protonation changes in response to potassium, which modulates its Förster radiative energy transfer rate and the corresponding interaction radii with each quantum dot species. Finally, the nanosensors respond to changes in potassium concentrations typical of the cellular environment and thus provide a promising tool for imaging potassium fluxes during biological events.},
doi = {10.1021/acsnano.5b05396},
journal = {ACS Nano},
number = 4,
volume = 10,
place = {United States},
year = {2016},
month = {4}
}

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Cited by: 18 works
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Works referencing / citing this record:

Ratiometric optical nanoprobes enable accurate molecular detection and imaging
journal, January 2018

  • Huang, Xiaolin; Song, Jibin; Yung, Bryant C.
  • Chemical Society Reviews, Vol. 47, Issue 8
  • DOI: 10.1039/c7cs00612h

Ratiometric optical nanoprobes enable accurate molecular detection and imaging
journal, January 2018

  • Huang, Xiaolin; Song, Jibin; Yung, Bryant C.
  • Chemical Society Reviews, Vol. 47, Issue 8
  • DOI: 10.1039/c7cs00612h