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Title: Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs

Abstract

Aphid infestation of switchgrass (Panicum virgatum) has the potential to reduce yields and biomass quality. Although switchgrass-greenbug (Schizaphis graminum; GB) interactions have been studied at the whole plant level, little information is available on plant defense responses at the molecular level. The global transcriptomic response of switchgrass cv Summer to GB was monitored by RNA-Seq in infested and control (uninfested) plants harvested at 5, 10, and 15 days after infestation (DAI). Differentially expressed genes (DEGs) in infested plants were analyzed relative to control uninfested plants at each time point. DEGs in GB-infested plants induced by 5-DAI included an upregulation of reactive burst oxidases and several cell wall receptors. Expression changes in genes linked to redox metabolism, cell wall structure, and hormone biosynthesis were also observed by 5-DAI. At 10-DAI, network analysis indicated a massive upregulation of defense-associated genes, including NAC, WRKY, and MYB classes of transcription factors and potential ancillary signaling molecules such as leucine aminopeptidases. Molecular evidence for loss of chloroplastic functions was also detected at this time point. Supporting these molecular changes, chlorophyll content was significantly decreased, and ROS levels were elevated in infested plants 10-DAI. Total peroxidase and laccase activities were elevated in infested plants at 10-DAImore » relative to control uninfested plants. The net result appeared to be a broad scale defensive response that led to an apparent reduction in C and N assimilation and a potential redirection of nutrients away from GB and towards the production of defensive compounds, such as pipecolic acid, chlorogenic acid, and trehalose by 10-DAI. By 15-DAI, evidence of recovery in primary metabolism was noted based on transcript abundances for genes associated with carbon, nitrogen, and nutrient assimilation. Extensive remodeling of the plant transcriptome and the production of ROS and several defensive metabolites in an upland switchgrass cultivar were observed in response to GB feeding. The early loss and apparent recovery in primary metabolism by 15-DAI would suggest that these transcriptional changes in later stages of GB infestation could underlie the recovery response categorized for this switchgrass cultivar. These results can be exploited to develop switchgrass lines with more durable resistance to GB and potentially other aphids.« less

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [4];  [5];  [6];  [2]; ORCiD logo [2]
  1. West Chester Univ. of Pennsylvania, West Chester, PA (United States). Dept. of Biology
  2. USDA Agricultural Research Service (ARS), Lincoln, NE (United States). Wheat, Sorghum, and Forage Research Unit
  3. USDA Agricultural Research Service (ARS), Lincoln, NE (United States). Wheat, Sorghum, and Forage Research Unit; USDA Agricultural Research Service (ARS), Manhattan, KS (United States). Stored Product Insect and Engineering Research Unit
  4. Univ. of Nebraska, Lincoln, NE (United States). Dept. of Entomology
  5. Univ. of Nebraska, Kearney, NE (United States). Biology Dept.
  6. Univ. of Nebraska, Lincoln, NE (United States). Dept. of Agronomy and Horticulture
Publication Date:
Research Org.:
USDA Agricultural Research Service (ARS), Lincoln, NE (United States); Univ. of Nebraska, Lincoln, NE (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Inst. of Health (NIH) (United States); USDA National Inst. of Food and Agriculture (NIFA) (United States)
OSTI Identifier:
1467064
Grant/Contract Number:  
AI02-09ER64829; SC0001490
Resource Type:
Accepted Manuscript
Journal Name:
BMC Plant Biology
Additional Journal Information:
Journal Volume: 17; Journal ID: ISSN 1471-2229
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; switchgrass; aphids; GB; ROS; RNA-Seq; metabolites; network; plant defense; pipecolic acid; chlorogenic acid

Citation Formats

Donze-Reiner, Teresa, Palmer, Nathan A., Scully, Erin D., Prochaska, Travis J., Koch, Kyle G., Heng-Moss, Tiffany, Bradshaw, Jeffrey D., Twigg, Paul, Amundsen, Keenan, Sattler, Scott E., and Sarath, Gautam. Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs. United States: N. p., 2017. Web. doi:10.1186/s12870-017-0998-2.
Donze-Reiner, Teresa, Palmer, Nathan A., Scully, Erin D., Prochaska, Travis J., Koch, Kyle G., Heng-Moss, Tiffany, Bradshaw, Jeffrey D., Twigg, Paul, Amundsen, Keenan, Sattler, Scott E., & Sarath, Gautam. Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs. United States. doi:10.1186/s12870-017-0998-2.
Donze-Reiner, Teresa, Palmer, Nathan A., Scully, Erin D., Prochaska, Travis J., Koch, Kyle G., Heng-Moss, Tiffany, Bradshaw, Jeffrey D., Twigg, Paul, Amundsen, Keenan, Sattler, Scott E., and Sarath, Gautam. Thu . "Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs". United States. doi:10.1186/s12870-017-0998-2. https://www.osti.gov/servlets/purl/1467064.
@article{osti_1467064,
title = {Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs},
author = {Donze-Reiner, Teresa and Palmer, Nathan A. and Scully, Erin D. and Prochaska, Travis J. and Koch, Kyle G. and Heng-Moss, Tiffany and Bradshaw, Jeffrey D. and Twigg, Paul and Amundsen, Keenan and Sattler, Scott E. and Sarath, Gautam},
abstractNote = {Aphid infestation of switchgrass (Panicum virgatum) has the potential to reduce yields and biomass quality. Although switchgrass-greenbug (Schizaphis graminum; GB) interactions have been studied at the whole plant level, little information is available on plant defense responses at the molecular level. The global transcriptomic response of switchgrass cv Summer to GB was monitored by RNA-Seq in infested and control (uninfested) plants harvested at 5, 10, and 15 days after infestation (DAI). Differentially expressed genes (DEGs) in infested plants were analyzed relative to control uninfested plants at each time point. DEGs in GB-infested plants induced by 5-DAI included an upregulation of reactive burst oxidases and several cell wall receptors. Expression changes in genes linked to redox metabolism, cell wall structure, and hormone biosynthesis were also observed by 5-DAI. At 10-DAI, network analysis indicated a massive upregulation of defense-associated genes, including NAC, WRKY, and MYB classes of transcription factors and potential ancillary signaling molecules such as leucine aminopeptidases. Molecular evidence for loss of chloroplastic functions was also detected at this time point. Supporting these molecular changes, chlorophyll content was significantly decreased, and ROS levels were elevated in infested plants 10-DAI. Total peroxidase and laccase activities were elevated in infested plants at 10-DAI relative to control uninfested plants. The net result appeared to be a broad scale defensive response that led to an apparent reduction in C and N assimilation and a potential redirection of nutrients away from GB and towards the production of defensive compounds, such as pipecolic acid, chlorogenic acid, and trehalose by 10-DAI. By 15-DAI, evidence of recovery in primary metabolism was noted based on transcript abundances for genes associated with carbon, nitrogen, and nutrient assimilation. Extensive remodeling of the plant transcriptome and the production of ROS and several defensive metabolites in an upland switchgrass cultivar were observed in response to GB feeding. The early loss and apparent recovery in primary metabolism by 15-DAI would suggest that these transcriptional changes in later stages of GB infestation could underlie the recovery response categorized for this switchgrass cultivar. These results can be exploited to develop switchgrass lines with more durable resistance to GB and potentially other aphids.},
doi = {10.1186/s12870-017-0998-2},
journal = {BMC Plant Biology},
number = ,
volume = 17,
place = {United States},
year = {2017},
month = {2}
}

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Works referenced in this record:

AtNAP, a NAC family transcription factor, has an important role in leaf senescence
journal, May 2006


    Works referencing / citing this record:

    Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs
    dataset, February 2017