Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein
Abstract
In this paper, we report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.
- Authors:
-
- Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics
- Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics and Department of Chemistry and Biochemistry
- Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry
- Publication Date:
- Research Org.:
- Univ. of California, Los Angeles, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1466773
- Grant/Contract Number:
- FC03-02ER63421
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ChemComm
- Additional Journal Information:
- Journal Volume: 52; Journal Issue: 28; Journal ID: ISSN 1359-7345
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Jorda, J., Leibly, D. J., Thompson, M. C., and Yeates, T. O. Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein. United States: N. p., 2016.
Web. doi:10.1039/c6cc00851h.
Jorda, J., Leibly, D. J., Thompson, M. C., & Yeates, T. O. Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein. United States. doi:10.1039/c6cc00851h.
Jorda, J., Leibly, D. J., Thompson, M. C., and Yeates, T. O. Wed .
"Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein". United States. doi:10.1039/c6cc00851h. https://www.osti.gov/servlets/purl/1466773.
@article{osti_1466773,
title = {Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein},
author = {Jorda, J. and Leibly, D. J. and Thompson, M. C. and Yeates, T. O.},
abstractNote = {In this paper, we report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.},
doi = {10.1039/c6cc00851h},
journal = {ChemComm},
number = 28,
volume = 52,
place = {United States},
year = {2016},
month = {3}
}
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 15 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Accurate design of co-assembling multi-component protein nanomaterials
journal, May 2014
- King, Neil P.; Bale, Jacob B.; Sheffler, William
- Nature, Vol. 510, Issue 7503
Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments
journal, February 2001
- Padilla, J. E.; Colovos, C.; Yeates, T. O.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 5
Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy
journal, May 2012
- King, N. P.; Sheffler, W.; Sawaya, M. R.
- Science, Vol. 336, Issue 6085
On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker
journal, August 2015
- Lai, Yen-Ting; Jiang, Lin; Chen, Wuyang
- Protein Engineering Design and Selection, Vol. 28, Issue 11
Features and development of Coot
journal, March 2010
- Emsley, P.; Lohkamp, B.; Scott, W. G.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
Bacterial Microcompartment Organelles: Protein Shell Structure and Evolution
journal, April 2010
- Yeates, Todd O.; Crowley, Christopher S.; Tanaka, Shiho
- Annual Review of Biophysics, Vol. 39, Issue 1
Structure and Mechanisms of a Protein-Based Organelle in Escherichia coli
journal, December 2009
- Tanaka, S.; Sawaya, M. R.; Yeates, T. O.
- Science, Vol. 327, Issue 5961
Protein-based organelles in bacteria: carboxysomes and related microcompartments
journal, August 2008
- Yeates, Todd O.; Kerfeld, Cheryl A.; Heinhorst, Sabine
- Nature Reviews Microbiology, Vol. 6, Issue 9
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Structure of a designed protein cage that self-assembles into a highly porous cube
journal, November 2014
- Lai, Yen-Ting; Reading, Eamonn; Hura, Greg L.
- Nature Chemistry, Vol. 6, Issue 12
Directed Evolution of a Protein Container
journal, February 2011
- Worsdorfer, B.; Woycechowsky, K. J.; Hilvert, D.
- Science, Vol. 331, Issue 6017
Quasi-equivalent viruses: a paradigm for protein assemblies 1 1Edited by T. Richmond
journal, July 1997
- Johnson, John E.; Speir, Jeffrey A.
- Journal of Molecular Biology, Vol. 269, Issue 5
Re-engineering protein interfaces yields copper-inducible ferritin cage assembly
journal, January 2013
- Huard, Dustin J. E.; Kane, Kathleen M.; Tezcan, F. Akif
- Nature Chemical Biology, Vol. 9, Issue 3
Diverse Bacterial Microcompartment Organelles
journal, September 2014
- Chowdhury, C.; Sinha, S.; Chun, S.
- Microbiology and Molecular Biology Reviews, Vol. 78, Issue 3
Structural Insights into Higher Order Assembly and Function of the Bacterial Microcompartment Protein PduA
journal, May 2014
- Pang, Allan; Frank, Stefanie; Brown, Ian
- Journal of Biological Chemistry, Vol. 289, Issue 32
Structure of a 16-nm Cage Designed by Using Protein Oligomers
journal, May 2012
- Lai, Y. -T.; Cascio, D.; Yeates, T. O.
- Science, Vol. 336, Issue 6085
Design of a single-chain polypeptide tetrahedron assembled from coiled-coil segments
journal, April 2013
- Gradišar, Helena; Božič, Sabina; Doles, Tibor
- Nature Chemical Biology, Vol. 9, Issue 6
Alanine Scanning Mutagenesis Identifies an Asparagine–Arginine–Lysine Triad Essential to Assembly of the Shell of the Pdu Microcompartment
journal, June 2014
- Sinha, Sharmistha; Cheng, Shouqiang; Sung, Yea Won
- Journal of Molecular Biology, Vol. 426, Issue 12
Structural Symmetry and Protein Function
journal, June 2000
- Goodsell, David S.; Olson, Arthur J.
- Annual Review of Biophysics and Biomolecular Structure, Vol. 29, Issue 1
Minimalist Representations and the Importance of Nearest Neighbor Effects in Protein Folding Simulations
journal, November 2006
- Colubri, Andrés; Jha, Abhishek K.; Shen, Min-yi
- Journal of Molecular Biology, Vol. 363, Issue 4
XDS
journal, January 2010
- Kabsch, Wolfgang
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009
- Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
- Nature Methods, Vol. 6, Issue 5, p. 343-345
Creating regular arrays of nanoparticles with self-assembling protein building blocks
journal, January 2007
- Howorka, Stefan
- Journal of Materials Chemistry, Vol. 17, Issue 20
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Structure of the PduU Shell Protein from the Pdu Microcompartment of Salmonella
journal, September 2008
- Crowley, Christopher S.; Sawaya, Michael R.; Bobik, Thomas A.
- Structure, Vol. 16, Issue 9
Structural Insight into the Mechanisms of Transport across the Salmonella enterica Pdu Microcompartment Shell
journal, September 2010
- Crowley, Christopher S.; Cascio, Duilio; Sawaya, Michael R.
- Journal of Biological Chemistry, Vol. 285, Issue 48
Comparative Protein Structure Modeling Using MODELLER
journal, September 2014
- Webb, Benjamin; Sali, Andrej
- Current Protocols in Bioinformatics, Vol. 47, Issue 1
Works referencing / citing this record:
Functionalization of Bacterial Microcompartment Shell Proteins With Covalently Attached Heme
journal, January 2020
- Huang, Jingcheng; Ferlez, Bryan H.; Young, Eric J.
- Frontiers in Bioengineering and Biotechnology, Vol. 7
Engineering the Bacterial Microcompartment Domain for Molecular Scaffolding Applications
journal, July 2017
- Young, Eric J.; Burton, Rodney; Mahalik, Jyoti P.
- Frontiers in Microbiology, Vol. 8
De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments
journal, August 2018
- Lee, Matthew J.; Mantell, Judith; Brown, Ian R.
- Nature Communications, Vol. 9, Issue 1
Tailoring lumazine synthase assemblies for bionanotechnology
journal, January 2018
- Azuma, Yusuke; Edwardson, Thomas G. W.; Hilvert, Donald
- Chemical Society Reviews, Vol. 47, Issue 10
Construction of a Triangle-Shaped Trimer and a Tetrahedron Using an α-Helix-Inserted Circular Permutant of Cytochrome c 555
journal, March 2018
- Oda, Akiya; Nagao, Satoshi; Yamanaka, Masaru
- Chemistry - An Asian Journal, Vol. 13, Issue 8
Encapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles
journal, January 2020
- Kalnins, Gints; Cesle, Eva-Emilija; Jansons, Juris
- Nature Communications, Vol. 11, Issue 1
Self-Assembly Stability and Variability of Bacterial Microcompartment Shell Proteins in Response to the Environmental Change
journal, February 2019
- Faulkner, Matthew; Zhao, Long-Sheng; Barrett, Steve
- Nanoscale Research Letters, Vol. 14, Issue 1
Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
journal, April 2018
- Hartman, Emily C.; Jakobson, Christopher M.; Favor, Andrew H.
- Nature Communications, Vol. 9, Issue 1