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Title: Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein

Abstract

In this paper, we report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.

Authors:
 [1];  [2];  [3];  [2]
  1. Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics
  2. Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics and Department of Chemistry and Biochemistry
  3. Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry
Publication Date:
Research Org.:
Univ. of California, Los Angeles, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1466773
Grant/Contract Number:  
FC03-02ER63421
Resource Type:
Accepted Manuscript
Journal Name:
ChemComm
Additional Journal Information:
Journal Volume: 52; Journal Issue: 28; Journal ID: ISSN 1359-7345
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Jorda, J., Leibly, D. J., Thompson, M. C., and Yeates, T. O. Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein. United States: N. p., 2016. Web. doi:10.1039/c6cc00851h.
Jorda, J., Leibly, D. J., Thompson, M. C., & Yeates, T. O. Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein. United States. doi:10.1039/c6cc00851h.
Jorda, J., Leibly, D. J., Thompson, M. C., and Yeates, T. O. Wed . "Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein". United States. doi:10.1039/c6cc00851h. https://www.osti.gov/servlets/purl/1466773.
@article{osti_1466773,
title = {Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein},
author = {Jorda, J. and Leibly, D. J. and Thompson, M. C. and Yeates, T. O.},
abstractNote = {In this paper, we report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.},
doi = {10.1039/c6cc00851h},
journal = {ChemComm},
number = 28,
volume = 52,
place = {United States},
year = {2016},
month = {3}
}

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Cited by: 3 works
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Works referenced in this record:

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
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Enzymatic assembly of DNA molecules up to several hundred kilobases
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