Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein

Jorda, J.; Leibly, D. J.; Thompson, M. C.; Yeates, T. O.

doi:10.1039/c6cc00851h

Title: Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein

Abstract

In this paper, we report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.

Authors:

Jorda, J. ^[1]; Leibly, D. J. ^[2]; Thompson, M. C. ^[3]; Yeates, T. O. ^[2]

Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics
Univ. of California, Los Angeles, CA (United States). UCLA-DOE Institute for Genomics and Proteomics and Department of Chemistry and Biochemistry
Univ. of California, Los Angeles, CA (United States). Department of Chemistry and Biochemistry

Publication Date:: 2016-03-09

Research Org.:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1466773

Grant/Contract Number:: FC03-02ER63421

Resource Type:: Accepted Manuscript

Journal Name:: ChemComm

Additional Journal Information:: Journal Volume: 52; Journal Issue: 28; Journal ID: ISSN 1359-7345

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Jorda, J., Leibly, D. J., Thompson, M. C., and Yeates, T. O. Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein.  United States: N. p., 2016. 
Web.  doi:10.1039/c6cc00851h.

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                    Jorda, J., Leibly, D. J., Thompson, M. C., & Yeates, T. O. Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein.  United States.  https://doi.org/10.1039/c6cc00851h

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                    Jorda, J., Leibly, D. J., Thompson, M. C., and Yeates, T. O. Wed .  
"Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein".  United States.  https://doi.org/10.1039/c6cc00851h.  https://www.osti.gov/servlets/purl/1466773.

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@article{osti_1466773,

  title        = {Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein},

  author       = {Jorda, J. and Leibly, D. J. and Thompson, M. C. and Yeates, T. O.},

  abstractNote = {In this paper, we report the crystal structure of a novel 60-subunit dodecahedral cage that results from self-assembly of a re-engineered version of a natural protein (PduA) from the Pdu microcompartment shell. Biophysical data illustrate the dependence of assembly on solution conditions, opening up new applications in microcompartment studies and nanotechnology.},

  doi          = {10.1039/c6cc00851h},

  journal      = {ChemComm},

  number       = 28,

  volume       = 52,

  place        = {United States},

  year         = {2016},

  month        = {3}

}

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Title: Structure of a novel 13 nm dodecahedral nanocage assembled from a redesigned bacterial microcompartment shell protein

Abstract

Citation Formats

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Accurate design of co-assembling multi-component protein nanomaterials
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Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments
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Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy
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On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker
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Features and development of Coot
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Protein-based organelles in bacteria: carboxysomes and related microcompartments
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PHENIX: a comprehensive Python-based system for macromolecular structure solution
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Structure of a designed protein cage that self-assembles into a highly porous cube
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Directed Evolution of a Protein Container
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Diverse Bacterial Microcompartment Organelles
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Structural Insights into Higher Order Assembly and Function of the Bacterial Microcompartment Protein PduA
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Structure of a 16-nm Cage Designed by Using Protein Oligomers
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Alanine Scanning Mutagenesis Identifies an Asparagine–Arginine–Lysine Triad Essential to Assembly of the Shell of the Pdu Microcompartment
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Structure of the PduU Shell Protein from the Pdu Microcompartment of Salmonella
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