How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells
Abstract
In this study, the effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as “sparging.” He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Finally, monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.
- Authors:
-
- Meijo University, Nagoya (Japan). Department of Electrical and Electronic Engineering
- Univ. of South Australia, Adelaide, SA (Australia). Future Industries Institute
- Univ. of South Australia, Adelaide, SA (Australia). Future Industries Institute; Lancaster Univ. (United Kingdom). Materials Science Institute
- Kochi University of Technology, Kami (Japan). Department of Electronic and Photonic Systems Engineering
- Kochi University of Technology, Kami (Japan). Department of Electronic and Photonic Systems Engineering and Center for Nanotechnology
- Univ. of South Australia, Adelaide, SA (Australia). Future Industries Institute; Wound Management Innovation Cooperative Research Centre (Australia)
- Kochi Medical School, Nankoku (Japan). Department of Urology
- Australian National Univ., Canberra, ACT (Australia). Space Plasma, Power and Propulsion Laboratory, Research School of Physics and Engineering
- Univ. of Liverpool (United Kingdom). Department of Electrical Engineering and Electronics
- Univ. of California, Berkeley, CA (United States). Department of Chemical and Biomolecular Engineering
- Publication Date:
- Research Org.:
- Univ. of Michigan, Ann Arbor, MI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Fusion Energy Sciences (FES)
- OSTI Identifier:
- 1465946
- Grant/Contract Number:
- SC0001939
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied Physics Letters
- Additional Journal Information:
- Journal Volume: 109; Journal Issue: 20; Journal ID: ISSN 0003-6951
- Publisher:
- American Institute of Physics (AIP)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 70 PLASMA PHYSICS AND FUSION TECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Oh, Jun-Seok, Strudwick, Xanthe, Short, Robert D., Ogawa, Kotaro, Hatta, Akimitsu, Furuta, Hiroshi, Gaur, Nishtha, Hong, Sung-Ha, Cowin, Allison J., Fukuhara, Hideo, Inoue, Keiji, Ito, Masafumi, Charles, Christine, Boswell, Roderick W., Bradley, James W., Graves, David B., and Szili, Endre J. How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells. United States: N. p., 2016.
Web. doi:10.1063/1.4967880.
Oh, Jun-Seok, Strudwick, Xanthe, Short, Robert D., Ogawa, Kotaro, Hatta, Akimitsu, Furuta, Hiroshi, Gaur, Nishtha, Hong, Sung-Ha, Cowin, Allison J., Fukuhara, Hideo, Inoue, Keiji, Ito, Masafumi, Charles, Christine, Boswell, Roderick W., Bradley, James W., Graves, David B., & Szili, Endre J. How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells. United States. https://doi.org/10.1063/1.4967880
Oh, Jun-Seok, Strudwick, Xanthe, Short, Robert D., Ogawa, Kotaro, Hatta, Akimitsu, Furuta, Hiroshi, Gaur, Nishtha, Hong, Sung-Ha, Cowin, Allison J., Fukuhara, Hideo, Inoue, Keiji, Ito, Masafumi, Charles, Christine, Boswell, Roderick W., Bradley, James W., Graves, David B., and Szili, Endre J. Fri .
"How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells". United States. https://doi.org/10.1063/1.4967880. https://www.osti.gov/servlets/purl/1465946.
@article{osti_1465946,
title = {How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells},
author = {Oh, Jun-Seok and Strudwick, Xanthe and Short, Robert D. and Ogawa, Kotaro and Hatta, Akimitsu and Furuta, Hiroshi and Gaur, Nishtha and Hong, Sung-Ha and Cowin, Allison J. and Fukuhara, Hideo and Inoue, Keiji and Ito, Masafumi and Charles, Christine and Boswell, Roderick W. and Bradley, James W. and Graves, David B. and Szili, Endre J.},
abstractNote = {In this study, the effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as “sparging.” He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Finally, monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.},
doi = {10.1063/1.4967880},
journal = {Applied Physics Letters},
number = 20,
volume = 109,
place = {United States},
year = {Fri Nov 18 00:00:00 EST 2016},
month = {Fri Nov 18 00:00:00 EST 2016}
}
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